cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
mkb.fkunpad@gmail.com
Editorial Address
Gedung Rumah Sakit Pendidikan Unpad/Pamitran Unpad (Teaching Hospital Building) Faculty of Medicine Unpad, 3rd Floor Jl. Prof. Eyckman No. 38 Bandung, 40161, Indonesia
Location
Kota bandung,
Jawa barat
INDONESIA
Majalah Kedokteran Bandung
Published by Universitas Padjadjaran
ISSN : 0126074X     EISSN : 23386223     DOI : 10.15395/mkb
Core Subject : Health,
Medicine & Pharmacology
Majalah Kedokteran Bandung (MKB)/Bandung Medical Journal publishes peer-reviewed original articles and case reports in basic medical research, clinical research, and applied medical science. This journal is published quarterly (March, June, September, and December) by Faculty of Medicine Universitas Padjadjaran. Articles are original research that needs to be disseminated and written in English. All submitted manuscripts will go through the double-blind peer review and editorial review before being granted with acceptance for publication.
Arjuna Subject : -
Articles 651 Documents
 24   25   26   27   28 
Kloning Gen Melanoma Antigen 1 (Mage-1) dari Jaringan Testis untuk mendapatkan Plasmid Rekombinan Mage-1 Mastutik, Gondo; I’tishom, Reny; Hardjowijoto, Sunaryo; Putra, Suhartono Taat
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (952.367 KB)

Abstract

Gen Melanoma antigen-1 (Mage-1) diekspresikan oleh sel spermatogonia jaringan testis normal dan diekspresikan 60−80% oleh liver penderita karsinoma hepatoseluler (KH). Ekspresi Mage-1 merupakan penanda untuk diagnosis KH serta prediktor kanker lambung dan kolorektal. Isolasi messenger ribonucleid acid (mRNA) Mage-1 dari jaringan liver penderita KH sulit dilakukan sehingga dilakukan isolasi mRNA Mage-1 dari jaringan yang mengekspresikan Mage-1, yaitu jaringan testis normal. Penelitian ini merupakan penelitian eksploratif yang dilakukan di Lembaga Penyakit Tropis Universitas Airlangga, Agustus 2006–Agustus 2008. Tujuan untuk mengkloning seluruh area koding gen Mage-1 dari jaringan testis pada vektor dan mendapatkan plamid rekombinan Mage-1. Isolasi seluruh area koding gen Mage-1 dilakukan dengan teknik semi-nested polymerase chain reaction (PCR). Seluruh area koding gen Mage-1 diisolasi, kemudian dikloningkan ke plasmid pET101/D-TOPO dan ditransformasikan ke Escherichia coli (E. coli) Top10 untuk mendapatkan plasmid rekombinan Mage-1. Panjang pET101/D-TOPO adalah 5.753pb dan area koding gen penyandi Mage-1 927 bp sehingga total panjang plasmid rekombinan 6.680 bp (5.753+927). Hasil analisis restriksi dengan EcoRV menunjukkan pita 4.230 dan 2.450 (4.230+2.450 = 6.680). Analisis sekuens gen Mage-1 dari testis mempunyai homologi 100% dengan sekuens M77481 serta NM_004988, dan 99% dengan BC01755. Simpulan, berdasarkan hasil analisis restriksi dan sekuens maka diperoleh plasmid rekombinan pETGM/MAGE1-Testis yang mengandung seluruh area koding gen Mage-1 dan dapat digunakan untuk pengembangan kit diagnostik karsinoma.  [MKB. 2015;47(4):199–206]Kata kunci:  Jaringan testis, karsinoma hepatoseluler, kloning, melanoma antigen-1, pET101/D-TOPOCloning of Melanoma Antigen 1 (Mage-1) Gene from Testicular Tissue to Obtain the Recombinant Plasmid Mage-1Melanoma antigen-1 (Mage-1) is expressed by spermatogonia cells of normal testicular tissue and 60−80% is expressed by the liver of hepatocellular carcinoma (HC) patients. Mage-1 expression is a marker for diagnosing HC and predicting gastric and colorectal cancers. Isolation of messenger ribonucleid acid (mRNA) Mage-1 from the liver tissue of HC patients is difficult; therefore, Mage-1 mRNA isolates can be obtained from tissues that express Mage-1 such as normal testicular tissues . This is an explorative research that was conducted at the Institute of Tropical Diseases of Airlangga University during August 2006–August 2008. The aim was to clone the coding sequence of Mage-1 gene from testicular tissues into a vector and to get recombinant plasmid Mage-1. Isolation of the full-length Mage-1 was performed using semi-nested polymerase chain reaction (PCR) which was then cloned into plasmid pET101/D-TOPO and transformed into Escherichia coli (E. coli) Top10 to get recombinant plasmid Mage-1. The length of pET101/D-TOPO was 5,753 bp and Mage-1 was 927 bp. The length of recombinant plasmid was 6,680 bp (5,753+927). Restriction analysis using EcoRV showed 4,230 and 2,450 bp bands (4,230+2,450=6,680). Sequence analyses showed that Mage-1 was 100% homologous with M77481 and NM_004988, 99% homologous with BC01755. In conclusion, according to the results of the restriction and sequences analysis, the recombinant plasmid pETGM/MAGE1-Testis contains the full length coding region of Mage-1 and is useful for developing the hepatocellular carcinoma diagnostic kits. [MKB. 2015;47(4):199–206] DOI: 10.15395/mkb.v47n4.621
Pengaruh Pemberian Vitamin D3 terhadap Kadar Reactive Oxygen Species (ROS) pada Sel PHM1-41 yang Mengalami Hipoksia Aziz, Muhammad Alamsyah; Krisnadi, Sofie Rifayani; Setiabudiawan, Budi; Handono, Budi
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n3.1408

Abstract

Kelahiran preterm (kurang bulan) merupakan salah satu penyebab kematian bayi yang hingga kini menjadi permasalahan di seluruh dunia. Salah satu mekanisme patofisiologis yang menyebabkan kelahiran kurang bulan adalah aktivitas sumbu hipotalamus-pituitari-adrenal (HPA) pada ibu dan janin. Stres maternal biologis berupa hipoksia merupakan salah satu penyebab terjadi mekanisme kelahiran kurang bulan melalui jalur aktivasi sumbu HPA ibu dan sebagai respons terhadap reactive oxygen species (ROS).  Vitamin D3 sebagai salah satu sumber ion Ca2+ dibutuhkan untuk mekanisme kontraksi dan relaksasi otot halus miometrium. Selain itu, vitamin D diduga berpengaruh terhadap kerja sumbu HPA. Tujuan penelitian ini adalah mengetahui pengaruh penambahan vitamin D3 pada sel lini PHM1-41 yang menjadi model in vitro dari kontraksi miometrium pada ibu hamil yang mengalami stres hipoksia terhadap kadar ROS intraseluler sel PHM1-41. Penelitian dilakukan di Laboratorium penelitian Aretha Medika Utama, Biomolecular and Biomedical Research Centre dengan kurun waktu penelitian dari bulan Desember 2017 hingga Februari 2018. Sel PHM1-41 yang telah dikultur dengan keadaan hipoksia selama 24 jam diberi penambahan vitamin D3, kemudian diukur kadar ROS intraselulernya. Hasil menunjukkan bahwa kadar ROS menurun signifikan pada kelompok sel yang diberi penambahan vitamin D3 dengan konsentrasi 150 nM dibanding dengan kelompok sel kontrol hipoksia. Hal ini menunjukkan  bahwa penambahan vitamin D3 150 nM memiliki potensi mencegah kelahiran kurang  bulanEffects of Vitamin D3 Treatment on Reactive Oxygen Species (ROS) Level in PHM1-41 Cell Line Experiencing HypoxiaPreterm birth is one of the major global cause of perinatal mortality. One of the pathophysiologic mechanisms leading to preterm birth is the Hypothalamic-Pituitary-Adrenal (HPA) axis activity of mother and fetus.. Maternal biological stress, such as hypoxia condition, is one of the trigger  of preterm birth through the activation of HPA axis as a response to the reactive oxygen species (ROS). Vitamin D3 as a source of Ca2+ ion is needed for myometrium smooth muscle’s contraction and relaxation mechanism. Vitamin D is also thought to strongly influence the HPA axis’s work. The purpose of this study was to determine the effect of  vitamin D3 provisionon PHM1-41 cell line induced by hypoxia as an  of pregnant women’s myometrium contraction through assessment of intracellular ROS level in PHM1-41 cell lines. This study was conducted in Aretha Medika Utama Biomolecular and Biomedical Research Centre from December 2017 to February 2018. PHM1-41 cells were cultured for 24 hours in hypoxia condition,Vitamin D3 was then added and the level of intracellular ROS was measured. Results showed that the ROS level decreased in cell clusters receiving 150nM vitamin D3 when compared to control hypoxia cell cluster. This indicates that the provision of 150nM vitamin D3 potentially prevents preterm  labor incidents.  
Karakteristik Atom Mineral pada Osteoporosis dengan Arsitektur Porosis dan Nonporosis Noor, Zairin; Sumitro, Sutiman B.; Hidayat, Mohammad; Rahim, Agus Hadian
Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.505 KB)

Abstract

Terdapat hasil yang bervariasi dalam peningkatan bone mineral density (BMD) akibat asupan kalsium mengindikasikan keterlibatan atom mineral lain dalam perkembangan struktur tulang. Kuantitas atom mineral tulang yaitu kadar atom mineral dalam struktur tulang. Penelitian ini bertujuan untuk membandingkan karakteristik atom mineral pada gambaran arsitektur (scanning electron microscope/SEM) porosis dibandingkan dengan nonporosis. Sepuluh penderita osteoporosis dengan gambaran SEM porosis dan tujuh penderita gambaran SEM nonporosis terlibat dalam penelitian ini. Tulang yang diambil ketika operasi fraktur dianalisis mikrostruktur dengan SEM dan karakter atom mineral tulang dengan X-ray fluorescence (XRF). Pemeriksaan SEM dan XRF dilaksanakan di Laboratorium Fisika Universitas Negeri Malang. Penelitian dilakukan mulai Agustus 2010 sampai Januari 2011. Uji t dilakukan untuk menentukan perbedaan kadar mineral tulang. Hasil SEM menunjukkan perbedaan porositas dan integritas trabekular antara SEM porosis dan SEM nonporosis. Analisis XRF didapatkan komposisi atom yang lebih tinggi pada tulang SEM nonporosis yaitu P, S, Fe, Ni, Cr, Pb, dan Si. Sebaliknya, komposisi atom yang lebih tinggi pada tulang SEM porosis adalah Cu, Zn, Ca, dan Mo. Perbedaan komposisi atom tersebut tidak berbeda secara bermakna (p>0,05). Simpulan, perkembangan porositas pada osteoporosis tidak didasari oleh kuantitas atom mineral tulang. [MKB. 2013;45(1):23–7]Kata kunci: Arsitektur, atom mineral, komposit, osteoporosisMineral Atomic Characteristic on Porotic and Non Porotic Architecture  of OsteoporosisThere was variance result in increased bone mineral density (BMD) due to calcium intake which indicates involvement of another mineral atomic in bone structure development. Bone mineral atomic quantity is the level or concentration of mineral atomic in bone structure. Aim of this study was to compare mineral atomic characteristic in architecture (scanning electron microscope/SEM) porotic and non porotic figure. Ten osteoporosis patients with porotic SEM and seven non porotic SEM patients were involved in this study. Bone which was obtained from fracture surgery was analyzed for microstructure by SEM and for bone atomic mineral characteristic by X-ray fluorescence (XRF). Analysis of SEM and XRF were done in Physics Laboratory State University of Malang. This study was done from August 2010 to January 2011. t-test was done to analyse the difference of atomic mineral level. Scanning electron microscope result showed the difference in porosity and trabecular integrity between porotic and non porotic SEM. Analysis of XRF showed atomic composition was higher in porotic SEM such as P, S, Fe, Ni, Cr, Pb and Si. Conversely atomic composition such as Cu, Zn, Ca, and Mo, was higher in non porotic SEM. There was no significant difference between each groups (p>0.05). In conclusion, the development of porocity in osteoporosis is not depend on bone mineral atomic quantity. [MKB. 2013;45(1):23–7]Key words: Architecture, composite, mineral atomic, osteoporosis DOI: http://dx.doi.org/10.15395/mkb.v45n1.96
Comparison of Knowledge, Attitude, Motivation-to-Breastfeed Exclusively between Allied Health Students and Students of Other Fields at Universitas Padjadjaran Astuti, Sri; Judistiani, Tina Dewi; Rahmiati, Lina; Susanti, Ari Indra
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.468 KB) | DOI: 10.15395/mkb.v48n3.848

Abstract

Comparison of Knowledge, Attitude, and Motivation-to-Breastfeed Exclusively between Allied Health Students and Students of Other Fields at Universitas PadjadjaranUniversity students are among future intellectuals who will play an important role in community education and empowerment, especially on the topic of exclusive breastfeeding, in which the coverage is low in Indonesia. This study aimed to compare students’ knowledge, attitude, and motivation-to-breastfeed exclusively between allied health studies (AHS), i.e medicine and midwifery,  and other fields studies (OFS), i.e. psychology, communication, literacy, and farming. Further information will be gained from exploring the correlation of knowledge-attitude, as well as attitude - motivation in both groups. A study was conducted among Indonesian female students in their final year of bachelor degree in October to November 2013. The subjects recruited were 196/340 female AHS and 300/633 female OFS. The remaining students were absent at data collection time. Validity and reliability of the questionnaires were satisfactory. Ethical clearance was obtained from the Committee on Health Research Ethics, Faculty of Medicine, Universitas Padjadjaran. The mean scores must be higher than the cut off points to pass. Student’s T test result showed a highly significant difference (AHS vs OFS group: 83.1 vs 71.1 for  knowledge, 91.3 vs 86.4 for attitude, and 88.3 vs 83.6 for  motivation-to-breastfeed exclusively,  p<0.001). It is revealed also that the correlation index between knowledge-attitude as well as attitude -  motivation-to- breastfeed exclusively were higher in AHS group, but some AHS students scored lower than the OFS students. Discussion on this topic is presented in this article. Corrective interventions are needed for students who scored low.  This study shows a satisfactory students’ scores of knowledge, attitude, motivation-to-breastfeed exclusively. The AHS group has a better performance and correlation index between  knowledge and attitude, attitude and  motivation-to-breastfeed exclusively. [MKB. 2016;48(3):176–80]Key words: Allied health students, attitude, knowledge, motivation-to-breastfeed exclusively, students of other fields Hubungan antara Pengetahuan dan Perilaku, serta Motivasi Menyusui pada Mahasiswi Fakultas Medis dan Non-medis di Universitas PadjadjaranMahasiswi universitas yang keberadaanya di antara para intelektual masa depan mempunyai peran penting dalam pendidikan dan pemberdayaan masyarakat, khususnya mengenai air susu ibu (ASI) eksklusif yang mempunyai cakupan rendah di Indonesia. Penelitian ini bertujuan membandingkan pengetahuan, sikap, dan motivasi untuk menyusui secara eksklusif antara beberapa studi kesehatan (AHS), yaitu kedokteran dan kebidanan, serta studi bidang lain (OFS), yaitu psikologi, komunikasi, sastra, dan pertanian. Informasi lebih lanjut akan mengeksplorasi hubungan pengetahuan, sikap, serta sikap-motivasi pada kedua kelompok. Penelitian ini dilakukan dari Oktober–November 2013. Sampel terdiri atas 196/340 perempuan AHS dan 300/633 OFS perempuan di Universitas Padjadjaran. Sampel yang tidak diambil adalah mahasiswi yang tidak hadir pada saat pengumpulan data. Validitas dan reliabilitas kuesioner yang memuaskan. Izin etik diperoleh dari Komite Etik Penelitian Kesehatan Fakultas Kedokteran Universitas Padjadjaran. Hasil nilai yang lebih tinggi mempunyai poin untuk lulus. Hasil uji-t mahasiswi menunjukkan perbedaan yang sangat signifikan (AHS vs kelompok OFS adalah 83,1 vs 71,1 untuk pengetahuan, 91,3 vs 86,4 sikap, dan 88,3 vs 83,6 motivasi untuk menyusui secara eksklusif, p<0,001). Indeks korelasi antara pengetahuan dan sikap serta sikap dengan motivasi untuk menyusui secara eksklusif lebih tinggi pada kelompok AHS, namun beberapa mahasiswi AHS mempunyai nilai lebih rendah daripada mahasiswi OFS. Hal tersebut dibahas dalam artikel, intervensi korektif yang diperlukan antara mahasiswi yang mendapat nilai rendah. Penelitian ini menunjukkan hasil yang memuaskan pada nilai mahasiswi tentang pengetahuan, sikap, dan motivasi untuk menyusui secara eksklusif. Kelompok AHS menunjukkan kinerja dan indeks korelasi yang lebih baik antara pengetahuan dan sikap, sikap dengan motivasi untuk menyusui secara eksklusif. [MKB. 2016;48(3):176–80]Kata kunci: Mahasiswi kesehatan, motivasi untuk menyusui secara eksklusif, mahasiswi non-kesehatan, sikap dan pengetahuan
Analisis Keberhasilan Terapi Bermain terhadap Perkembangan Potensi Kecerdasan Anak Retardasi Mental Sedang Usia 7–12 Tahun Lisnawati, Lilis; Shahib, M. Nurhalim; Wijayanegara, Hidayat
Majalah Kedokteran Bandung Vol 46, No 2 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1433.017 KB)

Abstract

Retardasi mental (RM) merupakan gangguan heterogen yang terdiri atas fungsi intelektual di bawah rata-rata disertai gangguan keterampilan adaptif. Terapi bermain merupakan pendekatan yang efektif untuk melatih anak RM taraf sedang dalam mempelajari suatu konsep pembelajaran. Terapi bermain dilakukan dalam ruang khusus yang didesain sebagai tempat bermain yang dilengkapi dengan perangkat mainan khusus untuk menstimulus perkembangan potensi anak RM taraf sedang. Tujuan dilakukannya penelitian ini untuk meningkatkan keberhasilan pengembangan potensi kecerdasan anak RM sedang dengan menggunakan instrumen The Wechsler-Intelligence Scale for Children (WISC) melalui penerapan terapi bermain.  Rancangan penelitian ini menggunakan metode eksperimen semu (quasi experiment) dan analisis kualitatif. Subjek dalam penelitian ini adalah anak RM sedang di SDLB Aisiyah usia 7–12 tahun sejumlah 13 anak. Pendekatan analisis yang digunakan adalah analisis statistik dengan pendekatan Wilcoxon dan Kruskal Wallis yang selanjutnya dilakukan analisis deskriptif untuk memberikan gambaran kondisi RM yang menyertai anak meliputi: faktor internal yaitu fase yang dialami anak pada masa kehamilan, persalinan, menyusui dan tahap tumbuh kembang, serta faktor eksternal yaitu kondisi sosial ekonomi keluarga dan pola asuh pada anak. Hasil penelitian 7 dari 13 anak RM sedang berhasil mengalami peningkatan dalam pengembangan potensi kecerdasannya. Bila dilihat dari hubungan frekuensi diberikannya terapi dengan tingkat keberhasilan anak, dari 7 anak RM sedang yang berhasil, 5 di antaranya termasuk kategori sering diberikan terapi bermain. Simpulan, terapi bermain mampu meningkatkan keberhasilan pengembangan potensi kecerdasan anak RM sedang. Keberhasilan tersebut berhubungan dengan frekuensi diberikannya terapi bermain dan didukung oleh kondisi penyerta (faktor internal dan eksternal) pada diri anak. [MKB. 2014;46(2):73–82]Kata kunci: Terapi bermain, kecerdasan, retardasi mental sedang Analysis of the Effectiveness of Play Therapy in Developing the Intelligence of 7–12 Years Old Children with Moderate Mental RetardationMental Retardation (MR) is a heterogeneous disorder that consists of lower than average intellectual function along with the disruption of adaptive skills. Play therapy is an effective approach to train children with moderate MR in studying the concept of learning. Play therapy is conducted in a special room designed as a playground, equipped with special toys to stimulate potential development of children with moderate MR. This research aimed to improve the success of the potential development of intelligence in children with moderate MR using WISC instrument through play therapy. The study design used quasi-experimental method (quasi-experiment) and qualitative analysis. The subjects of this study were thirteen 7–12 years old children with moderate MR in extraordinary primary school Aisiyah. The analysis approach used was statistical analysis with Wilcoxon and Kruskal Wallis approaches. A descriptive analysis was subsequently carried out to provide a snapshot of MR conditions that accompany the child including: internal factors, i.e. the phase experienced by the child during pregnancy, childbirth, breastfeeding and the stage of growth and development, and external factors i.e. the family's socioeconomic condition and children upbringing. The results showed that 7 out of 13 children with moderate MR had experienced an increase in the potential development of intelligence. In terms of the relation between the therapy frequency and the children success rate, 5 of 7 moderate MR children who were successful were in the category of frequent treatment of play therapy. In conclusion, play therapy can increase the potential for successful intelligence development of children with moderate MR. This success is associated with treatment frequency and is supported by the presence of concomitant conditions (internal and external factors) in children. [MKB. 2014;46(2):73–82]Key words: Play therapy, intelligence, moderate mental retardation DOI:  10.15395/mkb.v46n2.277
Gambaran Partisipasi Masyarakat terhadap Pengendalian Vektor Melalui Kajian Tempat Perkembangbiakan Aedes aegypti di Kota Bandung Faridah, Lia; Respati, Titik; Sudigdoadi, Sunarjati; Sukandar, Hadyana
Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.081 KB) | DOI: 10.15395/mkb.v49n1.986

Abstract

Pada tahun 2014, Bandung memiliki angka kasus demam dengue (DD) tertinggi dari 27 kabupaten/kota di Jawa Barat. Upaya pengendalian DD telah dilaksanakan sejak beberapa dekade yang lalu. Salah satu upaya adalah dengan mengeliminasi tempat perkembangbiakan nyamuk melalui peran serta masyarakat. Penelitian ini bertujuan menilai partisipasi masyarakat Kota Bandung dengan mengetahui tempat-tempat perkembangbiakan Aedes aegypti baik di dalam maupun di luar rumah. Sampling dilakukan di 16 kelurahan di Kota Bandung yang telah dipilih berdasar atas angka kejadian DD, kepadatan penduduk, ketinggian permukaan, dan status sosial-ekonomi periode 2015. Populasi penelitian adalah rumah-rumah yang terdapat di 16 kelurahan tersebut. Jumlah sampel penelitian ini adalah 1.983 rumah yang merupakan perwakilan dari tiap kelurahan. Sampling jentik dilakukan pada berbagai tempat penampungan air, baik penampungan alami maupun buatan di sekitar pemukiman penduduk. Jentik yang ditemukan dimasukkan ke dalam wadah dan dibawa ke laboratorium untuk diidentifikasi dan dihitung jumlahnya. Hasil menunjukkan tempat perkembangbiakan nyamuk yang paling dominan adalah bak mandi (50%), talang air (24%), dan dispenser (15%). Data entomologi diperoleh hasil House index (HI) 24%, Container index (CI) 12%, dan Breteau index (BI) 36%. Hasil tersebut menunjukkan masih kurangnya peran serta masyarakat untuk mencegah DD dengan membasmi tempat perkembangbiakannya dan Kota Bandung masih berpotensi untuk terjadi penyebaran penyakit DD. [MKB. 2016;49(1):42–7]Kata kunci: Aedes aegypti, Bandung, demam dengue, tempat perkembangbiakan, partisipasi masyarakat Community Participation on Vector Control Based on Aedes aegypti’s Breeding Sites in BandungIn 2014, Bandung has the highest number of Dengue Fever cases of 27 districts and cities in West Java. Dengue Fever control efforts have been implemented for several decades. One of the efforts is the eradication of the vector breeding site with community participation. The aim of this study was to assess community participation by identifying Aedes aegypti’s breeding sites, both indoor and outdoor, in Bandung area. Sampling was conducted on houses in 16 villages throughout Bandung area. The sampling points were selected according to the Dengue Fever event number, population density, height, and socio-economic status. The total sample points were 1983 houses. Larvae from sample points were collected from various water containments, both natural and manmade, around the settlement. The larvae samples were then brought to the laboratory to be identified and counted. Results indicated the dominant breeding sites were bathtub (50%), gutter (24%), and dispenser (15%). Entomological survey resulted in 24% HI, 12% CI, and 36% BI. This indicates the lack of community participation in preventing DF by eradicating vector’s breeding sites and Bandung is still potential for DF outbreak. [MKB. 2016;49(1):42–7]Key words: Aedes aegypti, Bandung, breeding site, dengue fever, community pasticipation
Kinetics of CD4+ T-Lymphocyte in Dengue Virus Infection Laksono, Ida Safitri; Intansari, Umi S.
Majalah Kedokteran Bandung Vol 46, No 4 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.467 KB)

Abstract

Cellular immunity plays an important role in viral infection. The activation of effector T-lymphocytes and the release of cytokines define the course of dengue viral infection. CD4⁺ T-lymphocyte induces the immunopathology via various mechanisms; however,  its kinetics in different onset of fever and disease severity are not fully understood. This study was conducted to observe the kinetics of CD4+ T-lymphocyte level among dengue infected patients and to obtain the absolute cell count and relative percentage of CD4+ T-lymphocyte. This was a descriptive study on thirty six patients who met the WHO-1997 criteria of dengue infection, hospitalized in Dr. Sardjito Hospital in the period of March to May 2009. The CD4+ T-lymphocytes were examined using the flow cytometer. The significance of median CD4+ T-lymphocytes among days were assessed using Kruskal-Wallis and Mann-Whitney SPSS 11 for Window. The absolute CD4+ T-lymphocyte was significantly different among days (p<0.05) between DF and DHF patients, while the relative CD4+ percentage count was not  different (p>0.05).  The Absolute CD4+ T-lymphocytes count was low in the beginning of the disease course and increased from the 2nd day of fever to the normal level on the 7th day. In conclusion, DF and DHF do not differ in the absolute CD4+ T-lymphocytes count as well as in the relative CD4+ T-lymphocytes percentage.Key words: CD4+ T-lymphocyte, dengue fever, dengue hemorrhagic fever, dengue virusKinetik Limfosit T CD4+ pada infeksi Virus DengueAbstrakImunitas seluler berperan penting dalam infeksi virus. Aktivasi efektor limfosit T dan terlepasnya sitokin merupakan gambaran utama perjalanan klinis infeksi virus dengue. Sel limfosit T CD4+ menginduksi imunopatologi melalui berbagai mekanisme, namun bagaimana kinetikanya pada hari demam yang berbeda dan derajat berat penyakit masih belum sepenuhnya dipahami. Penelitian ini bertujuan untuk mengamati kinetika sel limfosit T CD4+ pada pasien infeksi dengue dan mendapatkan kadar absolut serta persentase relatif limfosit T CD4+. Penelitian deskriptif ini mengikutsertakan tiga puluh enam pasien yang memenuhi kriteria WHO 1997 untuk infeksi dengue, dirawat di RSUP Dr. Sardjito antara Maret sampai Mei 2009. Kadar limfosit T CD4+ diperiksa menggunakan flow cytometer. Perbedaan median limfosit T CD4+ pada berbagai hari demam dianalisis menggunakan Uji Kruskal-Wallis dan Mann-Whitney SPSS 11 untuk Windows. Kadar absolut limfosit T CD4+ berbeda secara bermakna antara hari demam (p<0,05). Persentase relatif limfosit T CD4+ tidak berbeda bermakna antara demam dengue dan demam berdarah dengue (p>0,05). Kadar absolut limfosit T CD4+ rendah pada awal perjalanan penyakit, meningkat pada hari ke-2 demam dan mencapai nilai normal pada hari ke-7 demam. Demam dengue dan demam berdarah dengue tidak menunjukkan kadar absolut maupun persentase relatif limfosit T CD4+ yang berbeda bermakna. Kata kunci: Demam berdarah dengue, demam dengue, limfosit T CD4+, virus dengue DOI: 10.15395/mkb.v46n4.341
Perbedaan Kadar Vitamin E dan Tumor Necrosis Factor Alpha (TNF-α) berdasar atas Status Massa Lemak Pasien dalam Hemodialisis Kronik Supriadi, Rudi; Christina, Christina; Soelaeman, M. Rachmat
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.829 KB) | DOI: 10.15395/mkb.v49n3.1121

Abstract

Sindrom malnutrisi-inflamasi merupakan masalah pada pasien dalam hemodialisis. Peningkatan inflamasi menyebabkan risiko malnutrisi meningkat. Massa lemak menggambarkan perubahan status nutrisi yang lebih baik pada pasien hemodialisis (HD). Peningkatan TNF-α menyebabkan produksi ROS lebih tinggi dan akan ditangkap oleh vitamin E. Vitamin E dan TNF-α keduanya disimpan di massa lemak dan bersikulasi dalam darah. Penelitian ini bertujuan mengetahui perbedaan kadar vitamin E dan TNF-α pada dua kelompok massa lemak berbeda pasien dalam HD kronik. Penelitian ini merupakan penelitian observasional analitik dengan metode potong lintang pada 42 pasien pria yang menjalani HD kronik di RS Dr. Hasan Sadikin Bandung periode September–Oktober 2016. Massa lemak diperiksa dengan alat BIA (bioelectric impedance analyzer) dan dibagi ke dalam dua kelompok, yaitu lebih dan normal/kurang. Vitamin E dan TNF-α diperiksa dengan kromatografi dan ELISA. Analisis statistik menggunakan uji beda dua kelompok tidak berpasangan. Hasil penelitian menunjukkan kadar vitamin E lebih tinggi pada massa lemak lebih daripada normal/kurang (p=0,042). Kadar TNF-α lebih rendah pada massa lemak lebih daripada normal/kurang namun tidak bermakna (p=0,443). Subjek usia>55 tahun, kadar vitamin E lebih tinggi pada massa lemak lebih daripada normal/kurang (p=0,029). Massa lemak lebih dengan lama HD >24 bulan, kadar vitamin E lebih rendah daripada HD ≤24 bulan (p=0,005). Massa lemak normal/kurang dengan lama HD >24 bulan, kadar TNF-α lebih tinggi daripada HD ≤24 bulan (p=0,031). Penelitian ini menyimpulkan, kadar vitamin E lebih tinggi pada kelompok subjek dengan massa lemak lebih daripada normal/kurang. Kadar vitamin E pada massa lemak lebih dengan lama HD >24 bulan, lebih rendah daripada ≤24 bulan. Tidak terdapat perbedaan kadar TNF-α pada kedua kelompok massa lemak, namun kadar TNF-α lebih tinggi pada massa lemak normal/kurang dengan lama HD >24 bulan daripada ≤24 bulan. [MKB. 2017;49(3):192–8]Kata kunci: HD kronik, massa lemak, sindrom malnutrisi-inflamasi, TNF-α, vitamin E Difference between Vitamin E and Tumor Necrosis Factor Alpha (TNF-α) Levels based on Fat Mass Status in Chronic Hemodialysis PatientsMalnutrition-inflammation syndrome is a problem frequently found in patients who undergo chronic hemodialysis (HD). Increased level of inflammation has a higher risk for malnutrition. Fat mass shows better nutritional status changes in HD patients. TNF-α will cause increased ROS production and will be scavenged by vitamin E that are both saved in fat mass. This study aimed to determine the difference between vitamin E  and TNF-α levels in two fat mass groups of chronic HD patients. This study was a cross-sectional observational-analytic study on 42 male chronic HD patients at Dr. Hasan Sadikin General Hospital Bandung in the period of September–October 2016. Fat mass was measured by BIA and divided in two fat mass groups, higher and normal/lower mass groups. Vit E and TNF-α levels were measured by chromatography and ELISA. Non-paired groups difference test was used as the statistical analysis. The results showed a higher level of vitamin E in higher fat mass group than normal/lower group (p=0.042). TNF-α level tended to be lower in higher fat mass group than in the normal/lower group;  however, the difference was not significant statistically (p=0.443). Subjects who were  >55 years showed a higher level of vitamin E in hifher fat mass group than in normal/lower (p=0.029). The higher fat mass group with HD duration  >24 months showed lower vitamin E level than those with the HD duration ≤24 months (p=0.005). Normal/lower fat mass group with a HD duration of  >24 months showed a higher TNF-α level than≤24 months(p=0.031). In conclusion, in chronic HD patients the vitamin E level is higher in the higher fat mass group than in the normal/lower group. The vitamin E level in the higher fat mass with HD duration of  >24 months is lower than in the HD duration of  ≤24 months. There is no significant difference in TNF-α level in the two fat mass groups; however, the TNF-α level is higher in normal/lower fat mass group with a duration of HD of >24 months than those with a duration of ≤24 months. [MKB. 2017;49(3):192–8]Key words: Chronic HD, fat mass, malnutrition inflammation syndrome, TNF-α, vitamin E
Faktor yang Memengaruhi Kandungan E. coli Makanan Jajanan SD di Wilayah Cimahi Selatan Riyanto, Agus; Abdillah, Asep Dian
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1491.522 KB)

Abstract

Data dari Badan Pengawasan Obat dan Makanan (BPOM) menyatakan bahwa 45% produk pangan olahan di lingkungan Sekolah Dasar (SD) tercemar oleh bahan berbahaya mulai dari fisik, kimiawi, maupun mikrobiologi. Dari studi pendahuluan didapatkan bahwa masih banyak makanan jajanan di SD wilayah Cimahi Selatan yang tidak memenuhi syarat kebersihan makanan. Penelitian ini bertujuan untuk mengetahui faktor yang memengaruhi kandungan E. coli makanan jajanan SD. Desain penelitian yang digunakan potong lintang. Uji chi-kuadrat digunakan untuk menganalisis hubungan antar variabel dan analisis multivariat untuk melihat faktor dominan yang memengaruhi kandungan E. coli makanan jajanan dengan uji regresi logistik ganda. Sampel dalam penelitian ini sebanyak 96 pedagang makanan jajanan di SD, teknik pengambilan sampel dengan cara acak sederhana. Penelitian dilakukan di SD wilayah Cimahi Selatan November 2010−Januari 1011. Hasil penelitian didapatkan bahwa faktor yang memengaruhi kandungan E. coli makanan jajanan SD yaitu kebersihan orang yang mengolah makanan (p=0,013), peralatan (p=0,033), bahan makanan (p=0,050), dan sarana penjualan (p=0,050). Variabel yang tidak memengaruhi yaitu penyajian makanan (p=0,381), pada analisis multivariat sarana penjualan makanan mempunyai Prevalency Odd Ratio (POR) paling tinggi (16,8). Simpulan, faktor yang dominan memengaruhi kandungan E. coli makanan jajanan SD yaitu sarana penjualan makanan. [MKB. 2012;44(2):77–82].Factors Affecting E. coli Content of Street Food at Elementary Schoolin South CimahiBased on Food and Drug Control Agency (FDA) data stated that 45% processed food product at the elementary school environment were contaminated with physical, chemical and microbiological dangerous substances. From previous study it was stated that many street food at elementary school in South Cimahi area did not qualify for food hygiene. This research was aim to know the factors affecting E. coli content of street food at elementary schools. The research design used was cross sectional. Chi-square test was used for statistical analysis of correlation between variables and multivariate analysis to see the dominant factors affecting E. coli content of street food by using multiple logistic regression. The samples were 96 street food traders at elementary schools, chosen by simple random sampling. Study was conducted at elementary school in South Cimahi in period November 2010−January 2011.The results showed that factors which correlate with E. coli content of street food at elementary school were the food processor persons hygiene (p=0.013), instruments (p=0.033), food materials (p=0.050) and the trade facilities (p=0.050). The variable that had no correlation was the food service (p=0.381), in the multivariate analysis food trader facilities had the highest Prevalency Odd Ratio (16.8). In conclusion, dominant factor that correlated to E. coli content of street food is food trader facilities. [MKB. 2012;44(2):77–82]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.127
Pengaruh Heat Treatment untuk Mengembalikan Sifat Mekanik Kawat T-loop Segmental Stainless Steel terhadap Besaran Gaya yang Dihasilkan Lavina, Avi; Hambali, Tono S.; Thahar, Bergman; Mardiati, Endah
Majalah Kedokteran Bandung Vol 47, No 3 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (963.251 KB)

Abstract

Prosedur heat treatment dengan suhu dan teknik yang tepat pada pegas T-loop segmental stainless steel dapat mengembalikan sifak-sifat mekanik kawat yang menurun akibat prosedur pembengkokan kawat. Penelitian ini bertujuan membandingkan gaya yang dihasilkan oleh pegas T-loop segmental stainless steel tanpa dan dengan heat treatment pada suhu 4500C selama 15 menit,  dengan berbagai jarak aktivasi dan gable. Desain penelitian adalah eksperimental laboratoris murni secara in vitro. Penelitian dilaksanakan di Laboratorium Kimia Murni FMIPA ITB pada tahun 2006. Sampel adalah 50 buah pegas T-loop segmental dengan gable: 0°–0°, 7,5°–7,5°; 15°–15°; 22,5°–22,5°; 30–30°, terdiri atas 25 pegas tanpa  heat treated  dan 25 pegas dengan heat treated. Gaya diukur menggunakan autograph pada jarak tarik 1, 2, dan 3 mm, serta hasilnya dilakukan analisis statistik ANOVA dengan desain faktorial 2 x 3 x 5 dan 5 replikasi tiap sel serta uji posthoc Student Newman Keuls dan Tukey. Hasil uji statistik menunjukkan bahwa perlakuan heat treated dibanding dengan tanpa heat treated serta interaksi antara perlakuan heat treatment dan gable memberikan efek yang signifikan terhadap gaya yang dihasilkan (p<0,05). Simpulan, heat treatment pada T-loop stainless steel menghasilkan efek berupa penurunan besar gaya yang dihasilkan. [MKB. 2015;47(3):167–73]Kata kunci: Gaya, heat treatment, T-loopThe Effect of Heat Treatment on Mechanical Properties in Restoring Stainless Steel Segmental T-Loop towards the Force CreatedAbstractHeat-treatment procedure with  precise temperature and technique on the stainless-steel segmental T-loop retraction spring can restore the mechanical properties that decreases after a wire-bending process. The goal of this study was to compare the force produced by a stainless-steel segmental T-loop spring without and with heat-treatment on 4500C for 15 minutes, with various activation distance and gable. The design of the study was in-vitro laboratory experimental design. This study was performed at the Chemistry Laboratory of the Faculty of Science, ITB in 2006. Sample included 50 segmental T-loop springs with α and β angle of 0°–0°, 7.5°–7.5°, 15°–15°, and 22.5°–22.5°, and  30°–30° which consisted of 25 springs without heat-treatment and 25 springs with heat-treatment. Force was measured using autograph with a retraction distance of 1, 2, and 3 mm and the results were gained from ANOVA statistics analysis with a factorial design of 2x3x5 and 5 replications for each cell and posthoc Student Newman Keuls and Tukey test. The result of the statistic test showed that heat-treatment compared to no heat-treatment and interaction between heat-treatment and gable gives significant effect to the force created (p-value <0.05). In conclusion, the heat-treatment on the stainless steel T-loop produces an effect  to decrease the created force. [MKB. 2015;47(3):167–73]Key words: Force, heat-treatment, T-loop DOI: 10.15395/mkb.v47n3.597

Page 26 of 66 | Total Record : 651

 24   25   26   27   28 

Filter by Year

2012 2025


Reset
Filter By Issues
All Issue Vol 57, No 4 (2025) Vol 57, No 3 (2025) Vol 57, No 2 (2025) Vol 57, No 1 (2025) Vol 56, No 4 (2024) Vol 56, No 3 (2024) Vol 56, No 2 (2024) Vol 56, No 1 (2024) Vol 55, No 4 (2023) Vol 55, No 3 (2023) Vol 55, No 2 (2023) Vol 55, No 1 (2023) Vol 54, No 4 (2022) Vol 54, No 3 (2022) Vol 54, No 2 (2022) Vol 54, No 1 (2022) Vol 53, No 4 (2021) Vol 53, No 3 (2021) Vol 53, No 2 (2021) Vol 53, No 1 (2021) Vol 52, No 4 (2020) Vol 52, No 3 (2020) Vol 52, No 2 (2020) Vol 52, No 1 (2020) Vol 51, No 4 (2019) Vol 51, No 3 (2019) Vol 51, No 2 (2019) Vol 51, No 1 (2019) Vol 50, No 4 (2018) Vol 50, No 3 (2018) Vol 50, No 2 (2018) Vol 50, No 1 (2018) Vol 49, No 4 (2017) Vol 49, No 3 (2017) Vol 49, No 2 (2017) Vol 49, No 1 (2017) Vol 48, No 4 (2016) Vol 48, No 3 (2016) Vol 48, No 2 (2016) Vol 48, No 1 (2016) Vol 47, No 4 (2015) Vol 47, No 3 (2015) Vol 47, No 2 (2015) Vol 47, No 1 (2015) Vol 46, No 4 (2014) Vol 46, No 3 (2014) Vol 46, No 2 (2014) Vol 46, No 1 (2014) Vol 45, No 4 (2013) Vol 45, No 3 (2013) Vol 45, No 2 (2013) Vol 45, No 1 (2013) Vol 42, No 3 Vol 44, No 4 (2012) Vol 44, No 3 (2012) Vol 44, No 2 (2012) Vol 44, No 1 (2012) Vol 43, No 4 Vol 43, No 3 Vol 43, No 2 Vol 43, No 1 Vol 42, No 4 Vol 42, No 2 Vol 42, No 1 Vol 41, No 4 Vol 41, No 3 Vol 41, No 2 Vol 41, No 1 More Issue