cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Knowledge Management Department
Contact Email
kmd@biotrop.org
Phone
+62251-8323848
Journal Mail Official
biotropia@biotrop.org
Editorial Address
SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA
Location
Kota bogor,
Jawa barat
INDONESIA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 520 Documents
 42   43   44   45   46 
TEA SAPONIN MODULATES IN VITRO RUMEN FERMENTATION PROFILE AND REDUCES METHANE PRODUCTION: A META-ANALYSIS Yulianri Rizki Yanza; Nurhayatun Mahmudah; Cecep Hidayat; Anuraga Jayanegara; Ainissya Fitri; Fitri Ramadhani; Hindun Syarifah; Hasan Basri Jumin; Dedek Andrian; Melissa; Nurul Fauziah; Leo Adhar Effendi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.1.1805

Abstract

Saponins are secondary plant metabolites binding hydrophobic non-sugar components that can reduce ruminant methane (CH4) production. Previous studies revealed that tea saponin is one of the common sources of saponins used to reduce enteric CH4 production. However, the effect of different levels of dietary tea saponin on rumen fermentation remains inconclusive. This study aimed to investigate the effect of tea saponin on in vitro rumen fermentation and methane production through a meta-analysis approach. A total of 6 articles were selected and included in the meta-analysis database, continued by the statistical analysis using SAS software through mixed model formula. The results showed that tea saponin levels lowered in vitro organic matter degradability (IVOMD) and in vitro dried matter degradability (IVDMD; P=0.01) by a linear response. Furthermore, increased tea saponin levels in the diet reduced enteric CH4 emission expressed as CH4/DM substrate and CH4/IVOMD by a quadratic (P=0.01) and a linear response (P=0.04), respectively. Increased tea saponin levels also tended to decrease gas production linearly (P=0.08). Tea saponins inclusion also decreased pH levels linearly (P=0.04) but increased NH3 quadratically (P=0.01). Moreover, total VFA concentration in the rumen also raised quadratically, followed by the increased acetate (C2) and propionate (C3) proportion by a linear response (P<0.05). As a result, the C2/C3 ratio was decreased in a linear response (P=0.01) by the increased tea saponin levels, where the protozoa population was also diminished by a quadratic response (P=0.01). In conclusion, tea saponin levels directly reduced enteric CH4 production by diminishing microbial populations.
Identificaton and Diversity of Endophytic Fungi Associated with The Seagrasses of Cebu, Central Philippines Venus Kinamot; Alvin Monotilla
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.1.1861

Abstract

Endophytic fungi were poorly documented in the marine environment, especially in seagrasses regardless of their importance as sources of novel metabolites. In the Philippines, studies are dearth despite having large areas of seagrass meadows. Thus, this study was conducted to isolate and identify endophytic fungi associated with common seagrasses: Enhalus acoroides, Cymodocea serrulata, and Thalassia hemprichii from the Philippines by morphologic and molecular methods. Seven species were identified in this study: Aspergillus tamarii, A. ochraceopetaliformis, A. sydowii, Penicillium citrinum, Xylaria sp., Beauveria bassiana, and Eutypella sp. Morphologically, Aspergillus spp. had septate hyphae, biseriate conidiophore, and smooth to rough globose conidia. Penicillium had a green colony, biverticillate conidiophore, and smooth, globose conidia. Beauveria bassiana had white to cream colonies with irregular edges and a powdery appearance. The conidiogenous cells had zigzag rachis in which a chain of conidia emerged. Eutypella sp. had white, filariform, and plumose colony. Xylaria sp. had a white colony with conspicuous radial lines and a plumose margin.  Phylogenetic analysis using 80 ITS rDNA sequences by neighbor-joining revealed the clustering of our isolates with the closest match taxa in the same clade with 100% bootstrap value. The estimate of evolutionary divergence between our isolates and their closest match taxa by pairwise distance showed no nucleotide base substitution suggesting high sequence identity between sequences. The most diverse endophyte is Aspergillus as it is ubiquitously adapted to the marine environment. To our knowledge, this is the first report of these species colonizing the seagrasses from the Philippines.
DIVERSITY, ECOLOGY AND CONSERVATION STATUS OF NEPENTHES IN WEST SUMATRA PROVINCE, INDONESIA Muhammad Mansur; Andi Salamah; Edi Mirmanto; Francis Q Brearley
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1896

Abstract

Nepenthes is the largest carnivorous plant genus present in Indonesia. There are 39 species of Nepenthes pitcher plants recorded in Sumatra from lowland to montane forests, and 34 of them are endemic; this represents the greatest species diversity of Nepenthes after Borneo. Field studies were conducted in 2021 and 2022 to increase our knowledge of the diversity, habitats and distributions of Nepenthes in West Sumatra province. Twenty-three species of Nepenthes were recorded from the province, consisting of 15 highland species, 4 mid-elevation species and 4 lowland species. Ecophysiological studies conducted at Bukit Malalak showed clumped distributions of N. bongso, N. dubia, N. eustachya and N. rhombicaulis. Foliar and pitcher fluid nutrient concentrations were found to be similar to those cited in other recent studies although growth rates were slightly more rapid than at Gunung Talang. Bukit Malalak is a new locality for two threatened species, namely N. dubia (CR) and N. rhombicaulis (VU), enlarging their extents of occurrence. In total, nine species from West Sumatra are threatened and conservation actions are urgently needed for these and other Nepenthes species remaining on the island.
USE OF BIOLOGICAL ORGANIC FERTILIZERS AND PESTICIDES TO IMPROVE POTATO CULTIVATION IN SLOPE ANDISOLS Tamad; Loekas Soetanto; Akhmad Rizqul Karim
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1902

Abstract

In the 1990s, potato yield in the Andisols of Dieng, Central Java, Indonesia, was approximately 30 t ha–1, but this value decreased rapidly to 12–15 t ha–1 in recent years. This rapid decline could be attributed to the use of unbalanced organic and chemical fertilizers, without the application of conservation techniques. Therefore, this study aimed to sustainably improve the local potato cultivation pattern of farmers on Andisols using biological organic fertilizers and pesticides (BOFP). A randomized block design was used with two factors, namely: 1) 20 t BOFP, 300 kg Urea, 500 kg SP 36, 300 kg KCl, and 200 kg lime ha–1, and 2) comparison with the pattern of farmers, consisting of 20 t of chicken manure, 1–t NPK, and 250 kg ZA ha–1. The potato plant mounds were tilted 10% to the contour direction, and each treatment was carried out with 16 replications. Granola seeds were used to plant potato during the rainy season from March-June 2022. The results showed that the plants cultivated using the local pattern of farmers were affected by wilt from Fusarium spp, while the use of BOFP decreased the incidence of the disease by 80%. Furthermore, the BOFP pattern significantly increased Andisols organic-C from 1.78% to 3.83% and total soil P from 5.20% to 11.34%, compared to the pattern of farmers. It also increased potato yields from 12.31 t ha–1 to 22.93 t ha–1 and the R/C from 0.85 to 1.23, compared to the pattern of farmers. Based on the results, the use of BOFP pattern decreased wilt attacks by Fusarium spp, improved the productivity of Andisols, as well as increased potato production and profits of farmers.
MARINE TURTLE CONSERVATION PROGRAM AT PUERTO PRINCESA SUBTERRANEAN RIVER NATIONAL PARK: CHALLENGES AND OPPORTUNITIES DURING COVID-19 PANDEMIC Liwayway Acero
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.1.1915

Abstract

The Puerto Princesa Subterranean River National Park (PPSRNP)-Marine Turtle Conservation Program (MTCP) can be found in West Coast of Palawan Philippines. Geographical location is located in the global center of marine biodiversity, the Coral Triangle. This study described the profile of PPSNRP-MTCP in terms of location, species of marine turtles, number of hatchlings released per year, management organizational structure, conservation activities and, determined the perceived management challenges and opportunities encountered by employees during COVID 19 pandemic. Documentary analysis, net-notgraphy and focal group virtual discussion and structured questionnaires were used in data gathering. There are 3 species that were recorded in the conservation site; Chelonia mydas, Eretmochelys imbricata and Lepidochelys olivacea. Hatchling success from 2015 to 2021 is at an average of 82%, and the mean hatchlings per year is 1659.4 (+/- sd.699.31). Lowest was 697 in 2014-2015, highest in 2019-2020 with 2,476 hatchlings. MTCP is under the supervision of Protected Area Superintendent (PASu). The management activities are; regular monitoring of egg nesting and hatching of the eggs, implementation of easement zone, community coastal clean-up, releasing of hatchlings and conservation awareness campaign. Debris left by typhoon Odette is the perceived very high challenge while the absence of noise during nesting season is perceived at a very high opportunity.
ECOLOGICAL EFFECTS OF BELIK RIVER HYDRAULIC CHARACTERISTICS ON PERIPHYTON COMMUNITY AS WATER QUALITY BIOINDICATOR Wahyu Sartika, Heni; Hadisusanto, Suwarno; Sulaiman, Muhammad
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.31.2.1644

Abstract

ARTICLE HIGLIGHTS- Differences in river hydraulic characteristics affect ecological river health- The influence of flooding on the dynamics of physical and chemical characteristics of river water- Life strategy, adaptation of periphyton to environmental changes- Development of green infrastructure for river conservationABSTRACTBelik River is one of the urban rivers in the Special Region of Yogyakarta which has hydrological problems both in quantity and quality. This triggered the construction of various types of channel modifications. This study aimed to identify water quality and the ecological effects of channel modifications based on the presence of periphyton communities as bioindicators. Sampling was carried out in January 2021 and March 2021. Samples were taken at 4 sampling sites with different channel characteristics with 3 repetitions at each location. The physico-chemical parameters measured included pH, detergent, nitrate, sulfate, total phosphate, TSS, TDS, DO, CO2, water temperature, depth, transparency, discharge, and current velocity. The results showed that the abundance of periphyton in January 2021 was higher than in March 2021. In January 2021 there were found 91 periphyton species dominated by Nitzschia improvisa, Nitzschia terricola, and Nitzschia philippinarum. Meanwhile, in March 2021 there were found 61 species of periphyton dominated by Nitzschia improvisa. Based on the results of Canonical Correlation Analysis (CCA), it was shown that in general the distribution and abundance of periphyton in the Belik River were influenced by CO2, total phosphate, discharge, DO, nitrate, TSS, and depth. The Shannon Wiener Diversity Index showed that water quality in January 2021 has decreased in March 2021. From the cluster analysis, it is known that there are differences in water quality and abundance of periphyton in the four types of river channels. It can be concluded that differences in hydraulic characteristics affected water quality and the ecological health of Belik River.
REMOVAL EFFICIENCY OF NITROGEN, PHOSPHORUS AND HEAVY METALS ASSOCIATED WITH SWINE WASTEWATER USING AQUATIC MACROPHYTES Chung Tan Nguyen; Ha Van Nguyen
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1038

Abstract

Wastes from breeding farms have globally increased greenhouse gases and caused a serious pollution to aquatic environments. Biogas treatment polymer bags could significantly reduce organic compounds; however, they could not effectively treat other pollutants in animal wastewater. The objective of this study was to assess removal efficiency of salinity and pollutants associated with pig wastewater using aquatic macrophytes. Four macrophytes namely Acrotichum aureum, Eleocharis dulcis, Typha domengensis, and Limnophyton obtusifolium and a soil control without vegetation were randomly assigned into fifteen mesocosms (1.2 x 0.7 x 0.6m) with 3 replicates for each treatment. Pig wastewater was filled continuously into input chambers of mesocosms in every three day with 5 liters. Water samples were collected from output chambers with 60 and 120 days after treatment while soil and vegetation samples were collected at the beginning and the end of the experiment. The results showed that E. dulcis, T. domengensis, and L. obtusifolium were dominant in removal of N, P, Cu and Zn and suspended solids as well; E. dulcis and T. domengensis significantly increased the dissolved oxygen; whereas the treatment ofL. obtusifolium species showed the best efficiency in salt-ion removal. Pollutants of N, P, Cu and Zn tend to accumulate more in the macrophyte roots than in their leaves. Accumulation of N, P, Cu and Zn in theL. obtusifolium’s biomass is the highest compared with other treatments. From findings, it is suggested that a combination of three aquatic macrophytes including E. dulcis, T. domengensis, and L. obtusifolium could establish a constructed wetland system to directly treat pollutants of livestock wastewaters
ELASTICITY ANALYSIS OF THE GRAZING AND DETRITAL PATHWAYS IN A SHALLOW PHILIPPINE SEAGRASS MEADOW Michael Clores
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1654

Abstract

Ecotrophic efficiency (EE) is an estimate of the proportion of production that is utilized by the next trophic level through direct predation or fishing or exported out of the ecosystem. In seagrass systems, analysis of EE provides crucial information on how biomass, when used or lost in biological functioning, affects the higher trophic levels via death or grazing relative to the energy lost via decomposition (i.e., Flow to the detritus, FTD) and exports to another ecosystem (i.e., Sum of all exports, SAE). In this study, projections on the effect of change in the EE of functional groups in seagrass systems due to the alteration of biomass were established heuristically using Elasticity Analysis. Using a previously constructed Ecopath model for a shallow Philippine seagrass meadow, the simulations of altering the biomass of seagrasses and their grazers were done to determine the change in EE, FTD, and SAE, thereby generating information on the dynamics of the grazing and detrital pathways in the seagrass ecosystem. Results showed the effects of biomass increase and decrease of grazers (herbivorous gastropods, Tripneustes gratilla, and polychaetes). If the grazers’ biomass increases, their EE tends to decrease, and biomass accumulation tends to increase. This implies that a fraction of their production used in the system is reduced even if their predators' density and feeding rate are still constant. In addition, the EE of seagrasses tends to increase, leading to a decrease in biomass accumulation at the primary producers’ trophic level. Lastly, the EE of detritus decreased because the FTD and SAE of its major contributors (the seagrasses) had also decreased. The findings contribute to the ongoing analysis of the role of herbivores versus detritivores in the energetics of seagrass habitats.
OPTIMIZATION OF Talinum paniculatum Gaertn. ROOT INDUCTION AND THE EFFECT OF PHOSPHATE CONCENTRATIONS AND AMMONIUM:NITRATE RATIO ON BIOMASS OF ADVENTITIOUS ROOTS IN IN VITRO CULTURE Yosephine Sri Wulan Manuhara; Palupi Dasawulan Lestari; Syifa Fajrisani; Putri Gehasti; Sugiharto
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1678

Abstract

Java ginseng (Talinum paniculatum Gaertn.) is a medicinal plant, the roots of which are commonly used in traditional medicine. In its natural habitat, the roots grow very slowly, requiring two to three years to produce 100 g of roots per plant. Plant tissue culture could therefore provide an alternative means of accelerating root growth. This research aimed to optimize root induction and determine the effect of phosphate (KH2PO4) concentration and the ratio between ammonium and nitrate (ammonium:nitrate) on the biomass of Java ginseng adventitious roots in in vitro culture. Stem and leaf were used as explants and various combinations and concentrations of IBA and BAP, kinetin, and TDZ were used as growth regulators. Leaf explants were grown in Murashige and Skoog (MS) media supplemented with IBA 2 mg/L and various concentrations of phosphate (170; 212.5; 255; 297.5; 340; 382.5; 425; 467.5; 510 mg/L) and various ammonium:nitrate ratios (21:19 mM as the control, 0:30 mM, 10:20 mM, 15:15 mM, 20:10 mM, 30:0 mM). Cultures were maintained for 6 weeks. The observed parameters were fresh weight, dry weight, the duration of root formation, and the number and length of adventitious roots. The data were analyzed using Analysis of Variance. The results showed that the concentration of phosphate and the ammonium:nitrate ratio significantly influenced the amount, length, fresh weight, and dry weight of Java ginseng adventitious root. The highest fresh weight (37.47 mg) and dry weight (5.53 mg) were achieved in the treatment of double phosphate concentration (KH2PO4 340 mg/L), while an ammonium:nitrate ratio of 10:20 mM was the optimum treatment to produce the highest biomass (fresh weight 73.6 mg and dry weight 8.2 mg).
PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani Ainutajriani; Sri Darmawati; Dewi Seswita Zilda; Muhammad Ardi Afriansyah; Ragil Saptaningtyas; Stalis Norma Ethica
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Abstract

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.

Page 44 of 52 | Total Record : 520

 42   43   44   45   46 

Filter by Year

1987 2025


Reset
Filter By Issues
All Issue Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025 Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025 Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024 Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024 Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024 Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023 Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023 Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023 Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022 Vol. 29 No. 2 (2022): BIOTROPIA Vol. 29 No. 2 Agustus 2022 Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022 Vol. 28 No. 3 (2021): BIOTROPIA Vol. 28 No. 3 December 2021 Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021 Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021 Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020 Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020 Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020 Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019 Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019 Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019 Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018 Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018 Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018 Vol. 24 No. 3 (2017): BIOTROPIA Vol. 24 No. 3 December 2017 Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017 Vol. 24 No. 1 (2017): BIOTROPIA Vol. 24 No. 1 April 2017 Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016 Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016 Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015 Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015 Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014 Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014 Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013 Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013 Vol. 19 No. 2 (2012): BIOTROPIA Vol. 19 No. 2 December 2012 Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012 Vol. 18 No. 2 (2011): BIOTROPIA Vol. 18 No. 2 December 2011 Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011 Vol. 17 No. 2 (2010): BIOTROPIA Vol. 17 No. 2 December 2010 Vol. 17 No. 1 (2010): BIOTROPIA Vol. 17 No. 1 June 2010 Vol. 16 No. 2 (2009): BIOTROPIA Vol. 16 No. 2 December 2009 Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009 Vol. 15 No. 2 (2008): BIOTROPIA Vol. 15 No. 2 December 2008 Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008 Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007 Vol. 14 No. 1 (2007): BIOTROPIA Vol. 14 No. 1 June 2007 Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006 Vol. 13 No. 1 (2006): BIOTROPIA Vol. 13 No. 1 June 2006 No. 25 (2005) No. 24 (2005) No. 23 (2004) No. 22 (2004) No. 21 (2003) No. 20 (2003) No. 19 (2002) No. 18 (2002) No. 17 (2001) No. 16 (2001) No. 15 (2000) No. 14 (1999) No. 13 (1999) No. 12 (1999) No. 11 (1998) No. 10 (1997) No. 9 (1996) No. 8 (1995) No. 7 (1994) No. 6 (1992) No. 5 (1992) No. 4 (1991) No. 3 (1989) No. 2 (1989) Vol. 1 No. 1 (1987) More Issue