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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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The Influence of Fermentation Time in the Physical and Chemical Composition of Fermented Soybean Husk by Using Aspergillus niger on the Quality of Raw Feed Materials Muhammad Ikhwan Ihtifazhuddin; Happy Nursyam; Arning Wilujeng Ekawati
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Soybean husk (Glycine max L. Merrill) a soybean processing waste as raw material for tempe obtained after the process of boiling and soaking soybeans. The main problem in the use of soybean husk (Glycine max L. Merrill) as feed material is its crude fiber content which is fairly high. This study aimed to observe the fermented soybean husk using Aspergillus niger to improve the quality of the raw feed materials. This was conducted by using completely randomized design (CRD) analysis and repeated three times; the time optimization of Aspergillus niger in 2, 4, and 6 days based on chemical analyses (moisture, protein, fat, ash, crude fiber and feed containing carbohydrates (NFE) and physical assessment fermentation (smell, texture, moisture and hyphae) were analyzed descriptive qualitatively. The results showed that 4 days fermentation of soybean husk using A. niger is successful gives the highest score based on physical characteristics texture, aroma, moisture, and the formed hyphae and the most effective treatment for decrease in crude fiber is 13% and increase in NFE contained in the largest on 4 days fermented soybean husk by Aspergillus niger with a long time 4 days. Keywords: Aspergillus niger, fermentation, soybean husk.

The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Harun Wijaya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.

Detection of VipAlbumin® Effect in CD34 and SDF-1 Mobilization, in Streptozotocin-induced Diabetes Mellitus Mice Andi Rizki Adi Pradana; Muhammad Sasmito Djati; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Diabetes mellitus is a disease in which the body loses its ability to provide tight regulation and maintain a dynamic interaction between the tissue sensitivity and insulin secretion by β cells. The impact of this dysfunctional mechanism is uncontrolled blood glucose levels that lead to hyperglycemia condition. Highly reactive free radicals have a strong involvement in the pathogenesis of diabetes mellitus, where one of its forming process may be triggered by hyperglycemia condition. Patients with diabetes mellitus itself vulnerable to endothelial dysfunction, which is caused by a decrease in circulating endothelial progenitor cells, and also a decrease in chemokines which play a role in affecting the activities of these cells. Hyperglycemia condition and free radical activity is a major cause of these endothelial progenitor cells dysfunction.The purpose of this study was to determine the role of VipAlbumin®, a supplement derived from Channa striatus albumin extracts in inhibiting the action of free radicals that are formed due to hyperglycemia condition, which can affect the increase in endothelial progenitor cells relative amount. This study used BALB/C mice that induced to undergo diabetes mellitus through streptozotocin injection intraperitoneally at 5-day old. Mice who have reached 4 week old and positive to diabetes mellitus (blood glucose levels > 200 mg.dl-1) will be administered with VipAlbumin® orally for 14 days. VipAlbumin® dosage was divided into 4 groups: positive control (without VipAlbumin®); 1st dose (0.01664 mg.gr-1 BW); 2nd dose (0.416 mg.gr-1 BW); 3rd dose (10.4 mg.gr-1 BW). The last step was flow cytometric analysis to determine the development of endothelial progenitor cells relative amount, which isolated from bone marrow. The variables measured in this study were the relative amount of CD34+ and SDF-1. Based to flow cytometric analysis, mice with VipAlbumin® administration did not show any significant improvement in CD34 relative amount when compared to the positive control. Relative amount of Chemokine SDF-1 itself, although only occur at the 3rd dose of VipAlbumin® treatment, has increased and significantly different from the positive control. Keywords: CD34, diabetes mellitus, free radicals, hyperglycemia, SDF-1, streptozotocin, VipAlbumin®

Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence Amira Baihani; Uun Yanuhar; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Koi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this research aims to understand the genetic variation and kindship of KHV isolate in the carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province based on thymidine kinase sequence. Water quality of fish pond was checked. KHV clinical symptom was checked visually in each fish sample, then sacrifices the organ (gill) for PCR analysis and DNA sequencing. The research results show that clinical symptoms that appear are pale fish gill and bleeding on the surface of the body. The temperature in the research site shows an optimum range for KHV development. The results electrophoresis of carp suspected with KHV shows five positives out of a total six samples. Genetically, KHV isolates from Lombok Island of West Nusa Tenggara Province is identical with the GenBank isolate code KHV-J/U/I and Indo_0C05WNT2.Keywords: Carp, Koi herpesvirus, Phylogenetic, Thymidine kinase.

Chaetoceros ceratosporum Diatomae in Feed Formula To Increase Growth and Post Larvae Immunity of Tiger Shrimp (Penaeus monodon Fab.) to Vibrio harveyi infection Arning Wilujeng Ekawati; Happy Nursyam; Edi Widjayanto; Marsoedi Marsoedi
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

This experiment aims to determine the effect and the best dose of Chaetoceros ceratosporum diatomae utilization in feed formula for post larvae of tiger shrimp (Penaeus monodon Fab.) growth and immunity to Vibrio harveyi infection. This research applied Completely Randomized Design (CRD) with 4 treatments and 3 replications. The treatment use Chaetoceros ceratosporum diatomae in feed formula (iso protein 39.02% and iso energy 3.58 kcal/g diet) in different doses, i.e. treatment A = 0 %; B = 3.04 %; C = 6.0 8%; D = 9.12 %. Observed parameters were Survival Rate, Growth Rate, Food Conversion Ratio (FCR) and Protein Efficiency Ratio (PER). Result showed that Chaetoceros ceratosporum diatomae utilization in feed formula affect the increase of growth and immunity of post larvae of tiger shrimp (Penaeus monodon Fab.) to Vibrio harveyi infection. The best dose in feed formula ranged from 5,75% - 5,95%. Key words: Chaetoceros ceratosporum, balance energy, feed conversion, protein efficiency, Vibrio harveyi, tiger shrimp

The Use of Full-Grained, Fragmented, and Reduced Marine Yeast Powder in Fodder Formulation to Improve the Growth of Eel Fish (Anguilla bicolor) Ria Retno Dewi Sartika Manik; Arning Wilujeng Ekawati; Hardoko Hardoko
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Ikan Sidat or eel fish (Anguilla bicolor) is a popular fish commodity in domestic trade as well as for export commodity. The use of marine yeast as fodder suffers from some weaknesses since it contains nucleic acids and rigid thick cell walls that obstruct the absorbance of nutrition. Optimizing the use of marine yeast as fodder ingredients can be done by fragmenting and reducing the yeast. This research attempts at optimizing the use of marine yeast regarding its rate of growth, fodder efficiency and the survival rate of eel fish. This research employs the complete random design using 4 different treatments and 3 repetition. In the four treatments, the fodder contained protein (45%) and iso energy (3.600 kkal.kg-1 fodder), which were applied to the treatment A 0% (control), B 5% (complete), C 5% (fragmented) and D 5% (reduced). The parameters used in this study were survival rate, specific growth rate, ratio of fodder conversion, ratio of protein efficiency, energy retention, protein retention and rate of metabolism. The data of this study showed the best result in the treatment D, 5% (reduced), showing survival rate value of 95.56%, specific growth at 1.12, food conversion ratio of 2.27, protein efficiency ratio of 0.98, protein retention at 18.26%, energy retention of 16.57, and protein metabolism rate at 81.96%. Keywords: eel fish, growth, marine yeast.

Protein Structure and Function Analysis Method of Aminoacyl-tRNA Synthetase Cofactor and Biotinylation Effect: Journal Review Restu Nugraha; Chien Chia Wang; Widodo Widodo
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Protein has been known as an important macromolecule which has a vital role among the living organism. One of the most interesting protein is Arc1p, which is a yeast-specific tRNA-binding protein. Arc1p is a unique protein that has the ability to form a ternary complex with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm. This complex can significantly enhance the aminoacylation efficiency of these two aaRSs to their respective cognate tRNAs. Recently, it was found that Arc1p can be biotinylated via post-translational modification at Lys86 (K86) in the N-domain. Here, we try to figure it out what kind of method that will help to create some clear information both in structure and function of this protein, when mutations occur inside of the K86 site within SSKD motifs of Arc1p. Several methods to better understanding obviously about protein characteristics comprises protein structural analysis; such as gel mobility shift assay, CD Spectroscopy, and limited proteolysis; protein functional analysis, and in silico modeling.Keywords: Arc1p, biotinylation, function, in silico, structure.

Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like Silvi Ikawati; Yung Fu Yen
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

The promoter plays an important role in the regulation of gene expression. The problem is some of binary vector that absence from promoter at cloning site. The cauliflower mosaic virus (CaMV) 35S promoter is a strong and constitutive promoter that widely used to produce transgenic organisms. In this experiment the cauliflower mosaic virus (CaMV) 35S promoter was spliced at upstream of DhPEX11-like for driving downstream transgenes DhPEX11-like expression used the technique of Overlap Extension by The Polymerase Chain Reaction. In gene splicing, internal primers are used to amplify some overlapping regions of both genes and then these internal primers are combined with the external primers in PCR process which allows amplification of the entire region. In the experiment, the recombinant PCR successfully spliced the 35S-DhPEX11 gene. This method is simple, rapid and reduced reagents used because it does not need many vector constructions.Keywords: DhPEX11-Like, Gene splicing, Oligonucleotide, PCR, Promoter

Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) Andri Maulidi; Fatchiyah Fatchiyah; Amir Hamidy; Nia Kurniawan
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python.Keywords: microsatellite, Python, Sundaland.

Diatomae Chaetoceros ceratosporum dalam Formula Pakan Meningkatkan Respon Imun Seluler Udang Windu (Penaeus monodon Fab.) Arning Wilujeng Ekawati; Happy Nursyam; Edi Widjayanto; Marsoedi Marsoedi
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis yang terbaik pada pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan terhadap peningkatan respon imun seluler udang windu (Penaeus monodon Fab.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Sebagai perlakuan adalah pemanfaatan Chaetoceros ceratosporum dalam formula pakan iso protein 39,02% dan iso energy 3,58 kkal g pakan dengan dosis yang berbeda yaitu: A (0%), B (3,04%), C (6,08%) dan D (9,12%). Parameter yang diamati adalah total hemosit, total diferensial hemosit (hyaline, semi granular dan granular), dan aktivitas vibriocidal. Berdasarkan hasil penelitian menunjukkan bahwa pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan dapat meningkatkan respon imun seluler, dan dosis terbaik berkisar 5,15% - 6,51%. Kata kunci: Chaetoceros ceratosporum, respon imun seluler, udang windu (Penaeus monodon Fab.)

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Home Page

OAI Link

Editorial Team

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Reviewer

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA