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INDONESIA
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Core Subject : Science,
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Articles 2,170 Documents
SCN1A exon 26 variants in epilepsy and migraine patients Nova Dian Lestari; Endang Mutiawati; Ahmad Hamim Sadewa; Hasan Sjahrir; . Syahrul; Dessy Rakhmawati Emril; . Harapan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.556 KB) | DOI: 10.19106/JMedScie/005004201806

Abstract

Epilepsy and migraine are common neurological diseases in many populations. Mutation of the voltage gated natrium channel Nav1.1 (SCN1A) are important causes of different genetic epilepsies and can also cause familial hemiplegic migraine (FHM-III). This study aimed to identify SCN1A gene variation in patients with epilepsy and common migraine. Gene variation analysis of exon 26 of the SCN1A gene was carried out in 33 patients with epilepsy, 33 patients with migraine and 30 control individuals from Neurology Polyclinic at Dr. Zainoel Abidin General Hospital, Banda Aceh. The PCR and direct sequencing methods were performed in this study. SCN1A gene variations were identified in two epilepsy patients. These gene variations located at exon 26 were four silent mutations in patient E27 at position A4440T (Leu1480Leu), T4443C (Leu1481Leu), A5046G (Leu1682Leu) and C5121T (Asp1707Asp). One silent mutation in patient E30 at position G5505A (Glu1835Glu). None of these gene variations were identified in controls and patients with common migraine in this study. This study has identified 5 genetic variations of SCN1A in patients with epilepsy but not in common migraine. The mechanism and relationship between these variants and epilepsy need to be clarified.
The accuracy of fine needle aspiration biopsy to diagnose breast neoplasm Hifdza Faza Felisha; Hanggoro Tri Rinonce; Sumadi Lukman Anwar; Ery Kus Dwianingsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.264 KB) | DOI: 10.19106/JMedSci005103201907

Abstract

Breast lump is a very common complaint among women, especially during the reproductive year. Fine needle aspiration biopsy (FNAB) is a less invasive procedure. It is usually performed as an initial diagnosis prior to the operative procedure. The accuracy of the FNAB in Indonesia needs to be elaborated. The study aimed to evaluate the sensitivity and specificity of FNAB in diagnosing breast neoplasm. This is a retrospective study with cross sectional design, involving 145 patients with breast lump who underwent FNAB and histopathology examination in Dr. Sardjito General Hospital, Yogyakarta, from 2012 to 2014. Data analysis showed that female to male ratio was 23. 2:1 commonly occurred at 41-50 years old. Forty-one cases (28.28%) diagnosed as a benign lesion with fibrocystic changes as the most frequentcase (11.19%). The malignant case was 104 cases (71.72%) with ductal carcinoma as the highest case (51.49%). FNAB achieved a sensitivity of 85.58%, a specificity of 100% and a total accuracy of 89.66% in determining the benign or malignant breast lump. The accuracy, sensitivity and specificity of FNAB in diagnosing ductal carcinoma were 83.58%, 85.51% and 81.54%, respectively. The accuracy, sensitivity and specificity of FNAB to diagnose fibrocystic changes lesion were 85.82%, 26.67% and 93.28%, respectively. FNAB can be used as an alternative diagnostic tool to diagnose breast neoplasm. It provides rapid, cheaper, effective, valuable, and less invasive procedure in diagnosis of breast lump. 
The effect of L-arginine therapy on hepatocyte cells damage in mice (Mus musculus) model of preeclampsia S Soetrisno; Bambang Arinekso; Sri Sulistyowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 4 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.939 KB) | DOI: 10.19106/JMedSci005104201905

Abstract

Preeclampsia is one of the main contributors on morbidity and mortality of mother and fetus. Imbalance of body antioxidants and oxidants has a role on endothelial destruction causing endothelial dysfunction which leads to preeclampsia. L-arginine administration is indicated to be able to fix the remodelling process of hepatocytes which was damaged on preeclampsia. The aim of this study was to investigate the effect of L-arginine on hepatocyte cells damage on mouse (Mus musculus) model of preeclampsia. Experimental study was conducted in the Experimental Animal Warehouse and Biomedical Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya. A total of 30 pregnant mice were divided into three groups with 10 mice in each group i.e. normal, preeclampsia model and preeclampsia model with L-arginine therapy pregnant mice. Preparates were taken from dissected mice liver, with prior paraffin block and were then stained with hematoxylin-eosin. Results were analyzed semi quantitatively with Manja Roenigk hepar histology scoring method. Data were analyzed using Kruskal-Wallis and Man-Whitney test. Mean of hepar histopathology score of preeclampsia model with L-arginine therapy (40.80±1.17/µm2) was significantly lower compared with preeclampsia model pregnant mice (58.80±4.23/µm2) (p<0.05), however it was significantly higher compared with normal pregnant mice (18.40±2.41/µm2) (p<0.05). It was indicated that the L-arginine therapy on preeclampsia mouse model can reduce the score of hepar histopathology. In conclusion, L-arginine administration can reduce the hepatocyte cells damage of mouse due to preeclampsia.
Effect of tagitinin C isolated from Tithonia diversifoli (Hemsley) A Gray on migration activity and TGF-β1 levels on keloid fibroblast Elvira Santi; Mae Sri Hartati Wahyuningsih; Arief Budiyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.706 KB) | DOI: 10.19106/JMedSci005103201901

Abstract

Keloid is the formation of excessive scar tissue characterized by fibroblast hiperproliferations and collagen deposits that are similar with cancer cells. Tagitinin C is proven can inhibit proliferation and deposition of keloids collagen fibroblast. However, the mechanism of action of tagitinin C in migration activities and TGF-β1 levels of keloid fibroblasts has not been proved, yet. This study aimed to investigate the effects of tagitinin C isolated from Tithonia diversifoli (Hemsley) on migration activity and TGF-β1 expression of keloid fibroblast. This was quasi experimental study with post test only controlled group design using keloid fibroblasts isolated from keloid patients. The migration activity were performed by scratch assay and TGF-β1 levels were measured using an ELISA kits. Isolate tagitinin C was more active inhibit fibroblast keloid migration compare to the control groups (p<0.05) after 48 h incubation. TGF-β1 levels after incubation with isolate tagitinin C was lower then control group (p<0.05). In conclusion, isolate tagitinin C can inhibit migration and reduce TGF-β1 levels on keloid fibroblast
Genetic polymorphism in DNA base excision repair gene XRCC1among medical radiation workers Harry Nugroho Eko Surniyantoro; Yanti Lusiyanti; Wiwin Mailana; Devita Tetriana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 4 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.437 KB) | DOI: 10.19106/JMedSci005104201903

Abstract

X-rays repair cross-complementing group 1 (XRCC1) gene is one of the gene that plays an important role in base excision repair system (BER) and DNA repair both single and double strand breaks. Individuals with XRCC1 exon 10 (Arg399Gln) gene polymorphisms and carrying 399Gln allele variants (A allele) have a greater risk of DNA damage than their wildtype, 399Arg. The aim of this study was to examine the genotype frequencies of single nucleotide polymorphisms (SNPs) of XRCC1 exon 10 among medical radiation workers. This study involved 77 samples from several hospitals in Indonesia. Genotyping of XRCC1 exon 10 gene polymorphism was performed using PCR-RFLP. Individuals carryingA allele had lower frequency than that is carrying their wildtype of 399Arg (0.39 vs. 0.61). The results indicated that 39% of medical radiation workers had a risk of repair efficiency of DNA damage and might influence an individual's risk of cancer. Ionizing radiation induces many types of damage to DNA, requiring multiple repair pathways to restore genomics integrity. Other important genes/pathways, especially those for DNA double-strand break repair, might also play a role and should be further investigated. Furthermore, polymorphisms leading to inefficient DNA repair might also be associated with late reactions to radiotherapy.
Expression of circulating miR-200c and vascular endothelial growth factor-A (VEGF-A) mRNA as potential biomarker in human hepatocellular carcinoma Nanda Qoriansas; Dede Renovaldi; Juwita Raditya; Puji Lestari; Nur Signa Gumilas; . Suharno; Didik Setyo Heriyanto; Neneng Ratnasari; Sofia Mubarika H
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.108 KB) | DOI: 10.19106/JMedScie/005004201805

Abstract

Hepatocellular carcinoma (HCC) is a common liver disease that causes significant publichealth problems throughout the world, including in Indonesia. The HCC is the six mostcommon cancers and second cancer-related deaths among men in the world. Recentlyit was reported that the microRNA is an important player in hepatocarcinogenesis. Theexpression of MiRNA-200c is often regulated in primary HCC and HCC cell lines. Vascularendothelial growth factor-A (VEGF-A) is a regulator of angiogenesis that has been reportedas miR-200c target gene. This study was conducted to measure expression levels in miR-200c and mRNAVEGF-A and their potential role as biomarkers at HCC. A total of 36HCC patients and 36 healthy subjects were included in this study. The relative expressionof miRNA-200c and mRNA VEGF-A was quantified using reverse transcription real timequantitative PCR (qRT PCR). Relative expression was calculated using . Unpaired t-testwas used to compare the expression levels of circulating miRNA-200c and mRNA VEGF-Ain HCC patients and healthy subjects. Pearson test was used to determine correlationbetween circulating miR-200c expression and mRNA VEGF-A expression levels. Theexpression levels of circulating miR-200c in HCC patients were lower compared to healthysubjects although it was not significant (p = 0.258). Conversely, the expression levelsof circulating mRNA VEGF-A in HCC patients were significantly higher compared tohealthy subjects (p = 0.001). The relative expression levels of circulating miR-200c werenegatively correlated with mRNA VEGF-A in HCC patients. In conclusion, the expressionlevels of mRNA VEGF-A in HCC patients are significantly deregulated in compared tothat in healthy subjects. Negative correlation between circulating miRN-200c and mRNAVEGF-A expression levels are reported in HCC patients.
The analysis of cell damage of liver and kidney among alcoholics in Yogyakarta, Indonesia S Suhartini; Hendro Widagdo; Yudha Nurhantari; Idha Arfianti Wiraagni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.818 KB) | DOI: 10.19106/JMedSci005103201908

Abstract

Alcohol tends to disrupt the organs function of human body, even can cause serious and chronic damage. In Indonesia, the data on cell damage taken from organs including the livers and kidneys among alcoholics are still practically unknown. The aim of this study was to compare the differences of organs’ cell disturbance between alcoholics and non-alcoholics in Yogyakarta, Indonesia. A crosssectional study was conducted among 197 people in Yogyakarta, including 96 alcoholics and 101 non-alcoholics. The material of the study was taken from venous blood samples. A kinetic photometric test was conducted to obtain data on blood-chemical markers’ value of livers (SGOT, SGPT, and GGT), and kidneys (BUN and serum creatinine). The data werethen analyzed by Chi square test.From a total of 96 alcoholics, 83.6% are males and 16.4% are females who consumed alcohol for an average of 16 years, with 51.6% routinely consuming it daily. The kind of alcohol they consumed was single-brand (59.8%). Based on 25th percentile (GGT), on 50th percentile (SGOT, GGT), and on 75th percentile (SGPT, GGT), the alcoholics had higher proportion value of liver marker disturbance than non-alcoholics which was statistically significant (p<0.05). Based on the 50th and 75th percentiles, the alcoholics also had higher proportion value on kidney marker (BUN) damage than non-alcoholics which was statistically significant (p<0.05). There were significant differences in GFR values between males and females (p<0.05), males had kidney cell damage 7.9 times more than females. There was no significant difference in the value of GFR between alcoholics and non-alcoholics. In conclusion, the alcoholics hassignificantly higher proportion value of blood-chemical markers than that non-alcoholics. The GFR values between males and females are also significantly different, and males had kidney cell damage 7.9 times more than that of females.
Longevity and development of Aedes aegypti larvae to imago in domestic sewage water M Martini; Yuniar Triasputri; Retno Hestiningsih; Sri Yuliawati; Susiana Purwantisasi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 4 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.034 KB) | DOI: 10.19106/JMedSci005104201906

Abstract

Dengue Hemorrhagic Fever (DHF) caused by dengue virus transmitted by Aedes aegypti (Ae. Aegypti) that lives and breeds in clean water. The aim of the study was to analyze the difference of the longevity and development of Ae. aegypti larva in the difference pH of domestic sewage water. This experimental used post only control group design to analyze population of Ae. aegypti instar III larvae which was bred in the Entomology Laboratory of the Faculty of Public Health, Diponegoro University, Semarang, Central Java. The number of tested larvae was 25 per media with six replications. Thus, the total number of larvae was 750. Data were analyzed using Kruskal Wallis and followed by Mann Whitney test. The result showed that Ae. aegypti larva could survive and breed to be mosquitos in the domestic sewage water with various pH levels. There was significant difference between the number of larvae transforming to pupa (p=0.002), pupa to imago (p=0.001), and the number of survival imago until second week (p<0.001) in the domestic waste water with various pH levels. Other findings revealed that people tended to wash away larvae they found in the water, but still used the water for daily live. As a result, the larvae bred in the domestic sewage water. Therefore, elimination breeding place (EBP) program needs to be socialized to make people aware of either domestic waste water or domestic clean water.
mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1 and AKR1C2 in metastatic and non-metastatic prostate cancer patients Indrawarman Soerohardjo; Muhammad Puteh Mauny; Alharsya Franklyn Ruckle; Ahmad Zulfan; Raden Danarto; Didik Setyo Heriyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.129 KB) | DOI: 10.19106/JMedScie/005004201808

Abstract

The progression of prostate cancer (PCa) mainly occurs caused by androgens. There is a linkbetween intratumoral steroidogenesis and castration-resistant prostate cancer. This studyaimed to determine the mRNA expression of various steroidogenic enzymes (CYP17A1,CYP11A1, CYP19A1, HSD3B1, and AKR1C2) in metastatic and non-metastatic prostatecancer patients. This study was conducted at the Anatomical Pathology Laboratory andUrologi Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing,Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta from September-November 2017. Samples were taken from 30 paraffin blocks with adenocarcinoma ofprostate, stained with hematoxylin-eosin (HE) and then classified into metastatic and nonmetastaticgroups. Samples then underwent deparaffinization procedure and examinationof mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1, AKR1C2 genes usingReal-Time PCR. The mean mRNA expressions of CYP11A1, CYP17A1, CYP19A1,HSD3B1, and AKR1C2 genes in the metastatic adenocarcinoma prostate group were 7.08,10.11, 3.94, 4.84 and 3.58, respectively. In the non-metastatic group, the mean mRNAexpressions of CYP11A1, CYP17A1, CYP19A1, HSD3B1, and AKR1C2 genes were 4.62,9.45, 3.46, 2.68 and 4.92, respectively. The mean of mRNA expression of CYP11A1,CYP17A1, CYP19A1, and HSD3B1 genes were higher in the metastatic group than nonmetastaticadenocarcinoma prostate group. However, it was not statistically significant(p>0.05). The highest mRNA expression of steroidogenic enzymes was the CYP17A1gene. In conclusion, the mRNA expressions of CYP17A1, CYP11A1, CYP19A1, HSD3B1were higher in the metastatic prostate cancer patients compared to that in non-metastaticprostate cancer patients but statistically not significant.
Association between sexual orientation and sexual contact with the incidence of human immunodeficiency virus (HIV) infection in Dr. Sardjito General Hospital, Yogyakarta Imtihani, Hajar; Pudjiati, Angela Satiti Retno; Luthfiandi, Mochammad Rifky; Susetiati, Devi Artami
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.33 KB)

Abstract

Anal intercourse has been known to have a high risk of human immunodeficiency virus (HIV) transmission. The objective of this study was to evaluate the relationship between sexual orientation and the mode of sexual contact with the incidence of HIV infection. This was observational analytic study with cross sectional design. Subjects were new male patient who visited sexually transmitted infection (IMS) clinics at Dr. Sardjito General Hospital, Yogyakarta during 2016. Data were obtained from medical records. The HIV status was established from HIV rapid test and enzyme-linked fluorescent assay (ELFA). Data were analyzed using descriptive test and Pearson’s chi-squared test with significance level of p< 0.05. Among 167 subjects, 47.91% were in the age group 17 - 25 years old. Forty subjects (24%) had HIV positive status. The majority of subjects were heterosexuals (111 subjects (66.47%)), 43 subjects (25.75%) were homosexuals, and 13 subjects (7.8%) were bisexuals. HIV positive subjects were more common in homosexual groups than were heterosexual and bisexual (p

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