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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul Mahfut K; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.475

Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Isolasi Bakteri Pendegradasi Minyak Solar Dari Perairan Teluk Pare-Pare Hasyimuddin A; M Natsir Djide; M Farid Samawi
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 1 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i1.1119

Abstract

This study aims to acquire the diesel oil degrading microbia in Pare-Pare port. This study was conducted at the Laboratory of Microbiology, Department of Biology, Faculty of Science and Technology, UIN Alauddin Makassar. The method is descriptive exploratory study. The data was analyzed by tables and graphs. The results showed that three isolates of bacteria isolated from the Pare-Pare Bay that can degrade diesel oil as Bacillus sp., Psedomonas aeruginosa, and Alkaligenes feacalis. P. aeruginosa is the best bacteria in degrading diesel oil.
Induksi Umbi Mikro Kentang Secara In Vitro Pada Suhu Tinggi Dengan Beberapa Tuber Promoter Usman Kris Joko Suharjo; Bambang Gonggo Murcitro; Tunjung Pamekas; Haryuni Haryuni
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3435

Abstract

Potato microtubers are the miniature of conventional seed tubers grown in the field. Producing potato microtuber in vitro at high temperature by applying many kinds of tuber promoting substances is similar to producing potato in the field at low elevation by applying the same tuber promoting substances. The objective of this research was to evaluate the performance of seven tuber promoters in inducing potato microtubers at high temperatures. Micro cuttings (6 weeks old) were grown at the liquid media of MS for stolon induction. Six weeks afterward, the media were moved to microtuber inducing media supplemented with tuber promoters ((FD, FD+10 COU, FD+20 COU, FD+30 COU, FD+40 COU, FD+50 COU, and IPB). The media were incubated in the dark at 20 + 2oC and 29 + 2oC. The results showed that, in general, high temperature inhibited crop growth and tuber formation. Individually, tuber promoter formula significantly affected microtuber emergence, in which IPB showed the slowest treatment in producing microtubers, the smallest number of tuber produced, and the smallest size of tubers. The interaction between temperature and tuber promoters were significantly affected the tuber formation, in which the fastest tuber formation (7,0 days ) was found in FD + 40 COU and the highest  percentage of plant producing tubers was found in FD + 30 COU. Moreover, the highest number of microtuber and the heaviest weight of microtuber were found in FD (no Coumarin). It was safely concluded that all tuber promoters were best performed at low temperature. Should anyone wish to make those tuber promoters work better at high temperature, it is recommended to increase the concentration of Coumarin added to the formula of tuber promoting substances.
Kontaminasi Bakteri Pada Saus Tomat Mie Ayam di Pasar Condong Catur Sleman Yogyakarta Tahun 2013 Fitri Nadifah; Maria Yasintha Bhoga; Yuliana Prasetyaningsih
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 1 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i1.465

Abstract

The tomato sauce is a complementary food that is often served with other foods, such as chicken noodles. Various factors can be the cause of contamination of tomato sauce by microbes like bacteria. These factors include environmental conditions that are not clean or container that was left open. Bacterial contamination in tomato sauce can result not suitable for consumption. Given by the high public interest in the consumption of tomato sauce with chicken noodle, it is necessary to observe the bacterial contamination of chicken noodle tomato sauce. To determine the bacterial contamination in tomato sauce of chicken noodle in traditional market of Condong Catur, Sleman, Yogyakarta. The samples used were tomato sauces that are used by chicken noodle stalls in the Condong Catur traditional market. Sampling was also carried out by recording the environmental conditions stalls and tomato sauce serving. To determine the bacterial contamination, samples examined in the laboratory based on the total bacterial count method using plate count agar (PCA) media. Seven of the ten samples showed the total number of bacteria <104 CFU/ml, whereas the other three samples are not suitable for consumption by the total number of bacteria >104 CFU / ml. The main factors of high contamination is poor hygiene of chicken noodle stall environmental, tomato sauce containers that are less clean and are often left open.Keywords: tomato sauce, total bacteria
Pengaruh Suhu Dan Intensitas Cahaya Terhadap Pertumbuhan Jamur Tiram Di Tangerang Fenny Amelia; Jose Ferdinand; Klerenita Maria; Michel Geren Waluyan; Indah Juwita Sari
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3426

Abstract

Oyster mushroom is a mushroom that can be consumed, because it contains many nutrients such as vitamins, minerals, and amino acid. Nowadays, demand for oyster mushroom in Indonesia continues to increase, especially in the lowland like Tangerang. Knowing this, it needs effort to control the habitat that oyster mushrooms can be cultivated. This research was conducted by giving three treatments where the first treatment directly using sun light, the second treatment using a lamp with 5 watts power, and a third treatment using a lamp with 15 watts power. The data obtained are progress of baglog and temperature measurements in each treatment and then the data were analyzed using ANOVA test. With 5% significance level, research results between treatments using direct sunlight with the light from the lamp gives a significantly different effect, while between a lamp with 5 watt power and a lamp with 15 watt power do not give a significantly different effect. Best oyster mushroom growth obtained at treatment using a lamp with 5 watts power.
Pemeliharaan Ikan Lontok (Ophiocara porocephala Valenciennes, 1837) Sebagai Upaya Konservasi Dengan Pemberian Pakan Udang Kecepe (Acetes sp.) Khairul Khairul; Uswatul Hasan
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 2 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i2.4442

Abstract

The spangled gudgeon (Ophiocara porocephala Valenciennes, 1837) is one of the fish that live in the waters of Belawan and has important economic value to the community. However, lately the wild population continues to decline due to factors that supposedly over fishing, waters pollution and habitat degradation. Cultivation effort is one of the solutions to keep the preserved and decrease arrests in nature. The study of associated cultivation efforts need to be made through research. The purpose of this research is to know the frequency of feeding the shrimp kecepe (Acetes sp.) the right against the growth of absolute spangled gudgeon fish. The benefits of research as the basis for the management and preservation of fish decreased as well as the increasingly Spangled Gudgeon fish efforts do local fish conservation in Belawan Rivers. Results of the study showed the average length of the highest absolute growth was at the treatment D (1.10 cm), followed the treatment C (0.90 cm), treatment B (0.80 cm), and the lowest at the treatment A (0.73 cm). Analysis of variance results indicate that the Fh (8 **) > 0.05 Ft (4.07) means the treatment of natural feeding shrimp kecepe very real influence (highly significant) against the growth of the absolute length of the juvenile spangled gudgeon.
Pertumbuhan Tiga Kultivar Padi (Oryza sativa L.) Terhadap Aplikasi Pupuk Bio Cair dan Kondisi Tanah Pertanian Pasca Erupsi Merapi 2010 Dwi Umi Siswanti; Diah Rachmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 2 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i2.456

Abstract

The eruption of Mount Merapi at the end of 2010, which resulted in the destruction of land and crops are changing wetland into dry land. Sleman which is located south of Mount Merapi became one of the areas that most affected by the eruption of Merapi in 2010. It is necessary to improve the processing of soil physical properties of degraded areas and efforts to restore agricultural soil nutrient. Returning soil nutrients can be cultivated with the addition of organic material or the addition of microbes. The research aims to analyze the growth of three cultivars of rice (Oryza sativa L.) againstthe application of biofertilizer in paddy fields were affected by the eruption of Merapi in 2010. These three rice cultivars are Situbagendit, Inpari 20 and Ciherang. This research was conducted by applying the biofertilizer as much as 10 liters / ha were given three times during the vegetative phase, ie: 7, 21and 35 days after planting. The results of this research indicate that the three rice varieties ie: Situbagendit, Ciherang and Inpari 20 have an optimal growth and an increase in soil nutient content in biofertilizer application.Keywords: biofertilizer, degraded land, Merapi eruption, rice
Status Padang Lamun Pantai-Pantai Wisata di Pacitan Nurul Kusuma Dewi; Sigit Ari Prabowo
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 1 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.567

Abstract

The purpose of this research was to study the condition of seagrass communities at Pacitan coast. The study was conducted at Klayar, Srau, Teleng Ria, Pancer, Tawang, and Pidakan at Pacitan coast. The presence of seagrass communities was determined by the physical and chemical factors. Data were collected using quadrate plots with five transects. At each transect, data was collected using the quadrate plot, 0.5 m x 0.5 m, with 11 replications. On each plot, number of species, density, and their coverage were measured. Water quality such as light penetration, water level, current velocity, salinity, temperature, wave height, DO, pH, sediment texture, and nutrients of NH4, NO3, PO4, C-organic were also measured. The results showed, from 6 beaches studied, seagrass is only found in Tawang and Pidakan. There were 3 species of seagrass in Tawang beach, i. e Cymodocea serrulata, Thalassia hemprichii, and Halophila ovalis which were distributed unevenly, with 37,66 % coverage. In Pidakan beach, there is 1 species of seagrass, Thalassia hemprichii, with 30,89 % coverage. These variations were due to the physical and chemical factors in each location. T. hemprichii was the seagrass species which was tolerant to changes in environmental conditions. Light and substrate were as important factors that determined the presence and abundance of seagrass communities in Pacitan coastal waters.Keywords: coverage, light, seagrass, substrate, tolerant species
Pemanfaatan Sari Pati Umbi Gadung (Dioscorea hispida Dennst) Untuk Koagulasi Lateks Karet Alam (Hevea brasiliensis) Fitria Lestari; Yuli Febrianti; Joko Wiyono
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 1 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i1.4181

Abstract

This research aims to know the utilization of gadung (Dioscorea hispida Dennst) tuber concentrate on natural latex (Hevea brasiliensis) rubber coagulation. The design used in this study was completely randomized design (RAL) with 6 treatments and 4 replications consisting of control and treatment group with concentration of 20 ml, 40 ml, 60 ml, 80 ml and 100 ml. The parameters observed were latex coagulation by administering some concentrations of gadung tuber. One way Anava test with 0.05% significance level showed significant result that is Fcount = 53 and Ftable = 2,77. This shows that gadung tuber concentrate gives a very real effect on latex coagulation. Thus it can be concluded that there is the effect of gadung tuber concentrate to natural rubber latex coagulation.
Analisis Multidrug Resistensi Terhadap Antibiotik Pada Salmonella typhi Dengan Teknik Multiplex PCR Andi Evi Erviani
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 1 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i1.447

Abstract

This research is about the analysis of resistance multidrug for antibiotic of Salmonella typhi with multiplex technique of PCR. This research purpose conducted to determine the presence of resistance multidrug for kloramfenikol and ampicillin of Salmonella typhi using multiplex technique of PCR. Testing of resistance multidrug performed using disc diffusion test and detection of resistance gene for kloramfenikol (cat P) and ampicillin (tem) with molecular technique using multiplex of PCR. Result of research indicate the suitability between resistance test using disc diffusion and detection resistance gene with multiplex of PCR, which is based on result of disc diffusion test and multiplex of PCR have occurred of resistance multidrug for kloramfenikol and ampicillin antibiotics, amount 10 patients (33.3%), resistance of one antibiotics amount 10 patient (33.3%) and non-resistance (sensitive) for both antibiotic amount 10 patient (33.3%).Keywords: Antibiotics, Multidrug, Resistance, Salmonella typhi

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