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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Variasi Genetik Attacus atlas L. (Lepidoptera: Saturniidae) Berdasar Penanda Molekuler ISSR (Inter Simple Sequence Repeat) Auronita Puspa Pratiwi
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 1 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i1.464

Abstract

Attacus atlas is one of potent silk-producing insect and possesses a significant economic value. A. atlas is part of genus Attacus which inhabits in several quite large areas. A.atlas can be found in Sumatera, Java, Borneo, Halmahera Islands, and Papua. Mostly, species which inhabits in wide spread area, has a high level of diversity. Applied studies on Antheraea dan Samia cynthia ricini showed that geographical differences led to genetic variations occurred upon those species. The aim of this research was to study and analyze the genetic variation A. Atlas, which is obtained from multiple sampling locations, ie Berbah, Dlingo, Ngawen, and Pracimantoro, based on ISSR marker. Analysis of genetic variation performed with 4 ISSR primer, ISSR1, ISSR2, ISSR6, and ISSR7 gave forth high genetic variations among individuals of A.atlas by indicating the average polymorphic percentage of all primer of 97,78%. Clustering analysis with unweighted pair group with arithmetic average (UPGMA) method resulted dendrogram that exhibiting similarity values among individuals in range between 61% - 87% and clustered into two main clusters at 61% similarity value. The first cluster consists of samples taken from from Dlingo, Ngawen and Pracimantoro which has elevation above 200 m asl, while the second cluster consists of samples originating from Berbah which has elevation below 200 m asl. Amplification used 4 ISSR primer performed 2 monomorphic loci and 82 polymorphic loci. Monomorphic at 300 bp showed by ISSR2 and at 1800 bp showed by ISSR7 may be a candidate locus identifier species of A. atlas.Keywords: Attacus atlas, genetic variation, PCR-ISSR
Gambaran Darah Katak Fejervarya limnocharis di Lahan Pertanian yang Menggunakan Pestisida di Sumatera Barat Yelvita Sari; Djong Hon Tjong; Resti Rahayu
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 2 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i2.3245

Abstract

Fejervarya limnocharis are amphibians which generally can be found in agriculture area. The high intensity of pesticide application in agricultural area can lead to alteration of physiological aspects of organism inhabiting that area. Quantitative examination of hematology aspect can be conducted to evaluate physiological condition of species. This research was aimed to find out about hematology profile of F. limnocharis living in agriculture area using pesticide in West Sumatera. Thus, examinations of erythrocytes, leukocytes, hemoglobin concentrations, and hematocrit values collected from F. limnocharis living in three different areas have been conducted. The data gained have been compared with normal blood value of various amphibians species from previous research. The result acquired respectively for three locations show that total of erythrocytes were in normal range, which were 1,616 x 106/mm3; 1,539 x 106/mm3; and 1,712 x 106/mm3; hemoglobin concentration were lower than normal range, which constituted 4,06 g/dL; 4 g/dL; and 4,18 g/dL; hematocrit values have normal range, which made up 40,06 %; 40,14 %; and 45,7 %; total of leukocytes were higher than normal range, which were 27,450/mm3; 49,934/mm3; and 22,040/mm3. Usage of pesticide in agriculture area causes alteration of some hematological aspect.Keywords: Fejervarya limnocharis, hematology profile, pesticide, West Sumatera
Kapang Kontaminan Pada Ikan Toreh (Hyporhamphus affinis) yang dijual di Pasar Tradisional Kota Ternate Maluku Utara Nurhasanah Nurhasanah; Sundari Sundari
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 2 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i2.4340

Abstract

Dried fish from fish Julung (Hyporhamphus affinis) is one type of fish food products that are found in the traditional market of Ternate city. This product is commonly known as fish toreh. The main problem of this product is storage packaging by using an open bamboo set that can be contaminated by various microbes. The purpose of this study was to inventory and identification of contaminant molds isolated from dried fish of H. affinis on a 17-day storage period. The research was conducted in Mathematic and Natural Science laboratory by using dried fish smoke (toreh) from H. affinis obtained from traditional market in Ternate city. Isolation and identification of molds using standard microbiological procedures. Media growth of mold using Potato Dextrose Agar (PDA). Several genus and species of mold found as contaminant mold are Aspergillus spp., Eurotium spp., Torula spp., Cladosporium spp., Polypaecilum pisce and Catenaria fuliginea.
Pola Hubungan Antara Jumlah Kelahiran Dengan Jumlah Betina dan Pejantan Produktif Pada Ternak Sapi Basir Paly
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 2 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i2.455

Abstract

This research aims to know the pattern of the relationship between the number of births and the number of males and females productive. Implemented in the Gowa in June-October 2014. Using method of survey through observation and interview. The population of the research was a wholegroup of cattle there. As many as 5 group of cattle has been observed and interviewed. Variables are observed and analyzed are: (1) the number of cattle owned by males and females, (2) the number of births, (3) the number of males and females productive, (4) sales, cut withholding, and cases of death,and (5) number of females productive female rescue. Data analysis was carried out in two categories, descriptive and inferensial. Descriptive analysis using a matrix, tabulate and a chart, while inferential analysis using SPSS and Amos 16 software, then validated with data and information from othersources. The results showed that there are four variables that are directly related to the number of births. Namely: (1) the number of female productive, (2) number of females sold, cut withholding, and death, (3) number of productive female rescue, and (4) the number of male productive. To four variables are also interrelated with one another, but not directly related to the number of births. This powerful relationship patterns indicate that the number of births is very closely related to the number of productive female rescue, and the number of males. While the weak relationship patterns indicate that the number of females sold, cut withholding, and the dead, is not related to the number of births.Keywords: birth, females, males, relationships
Epidemiologi, Stadium, dan Derajat Diferensiasi Kanker Kepala dan Leher Nelsiani To'Bungan; Siti Hamidatul A'liyah; Nastiti Wijayanti; Jajah Fachiroh
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 1 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.566

Abstract

Head and neck cancer is one of the deadly types of cancer in Indonesia. The main cause of this cancer is the consumption of alcohol and cigarettes. Head and neck cancer attacks the lips, mouth, palate, pharynx and larynx. Studies about head and neck cancer have been carried out in westerncountries, while in Indonesia is still limited. Earlier studies in western countries expressed that head and neck cancer is more common in men than women. Purpose of research were to determine the epidemiology of head and neck cancer in Indonesia related to patient ratio of men and women andthe correlation of stage and differentiation level of head and neck cancer. Data were obtained from the Rumah Sakit Kanker Dharmais-Pusat Kanker Nasional Jakarta and then analyzed descriptively. The data were analyzed came from 36 patients with head and neck cancer. The results showed, menwith head and neck cancer as much as 52,77% and females 47,22%. There were 16 cases with stage IV cancer, 9 with stage III, 8 with stage II and 2 with stage I. There were 6 cases of stage IV cancer with better differentiation, and there were 2 cases of stage II cancer with a poor differentiation. Head and neck cancer is more common in men than women. There was no correlation between the degree of differentiation-stage head and neck cancer. It was influenced by immunity of each person.Keywords: differentiation level, epidemiology, head and neck cancer, stage
Potensi Sari Pati Gadung (Dioscorea hispida L.) Sebagai Bioinsektisida Hama Walang Sangit Pada Tanaman Padi (Oryza sativa L.) Zico Fakhrur Rozi; Yuli Febrianti; Yardiani Telaumbanua
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 1 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i1.4185

Abstract

Walang sangit (Leptocorisa acuta L.) as a rice bug is a pest that can cause a decrease in rice production (Oryza sativa L.). Excessive use of chemical pesticides will cause resistance or immunity to the pest itself. Gadung (Dioscorea hispida L.) has a compound HCN (cyanide acid) that potentially cause metabolic disturbance in insects. This study aims to determine the extract of gadung tuber effect as bioinsecticide of rice bug against rice. This research was conducted in Biology Laboratory of STKIP PGRI Lubuklinggau South Sumatera Province, with 5 treatment groups and 5 replications. The research design used was complete randomized design (RAL) with P0 control (-) without giving of starch bulb, P1 20 gr / l, P2 40 gr / l, P3 60 gr / l, P4 80 gr / l. Based on the results of analysis with Anava One Path test obtained results Fcount = 7.72 with Ftable = 4.43. It shows there is a significant influence of the extract of gadung tuber as bioinsecticide of rice bug against rice plant.
Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA Ikhsan Nur Huda; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 1 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i1.446

Abstract

Melon (Cucumis melo L.) cultivars Gama Melon Basket (GMB) is the result of plant breeding that has the potential to be a product of local melon seed in Indonesia. This cultivar phenotype characters was known, but its genetic variation is not further studied. This study aimed to analyze the genetic variation of melon cultivars GMB and compared with 13 commercial melons and four members of the family Cucurbitaceae such as; cucumber, cucumber queen, barteh, and cantaloupe (blewah). RAPD-PCR method with 11 random primer was used to evaluate genetic variation in melon. Level of genetic similarity was determined by the formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis unweighted pair-group using arithmeticaverages (UPGMA). Results of RAPD-PCR with 11 primer produced 165 loci and it contains 84.85 % polymorphic DNA and 15.15 % monomorphic DNA. Results of genetic variation analysis suggests that 15 commercial melons differ from groups of cucumber, cucumber queen, barteh and cantaloupe(blewah). Cultivars GMB has genetic marker that can be distinguished from other commercial melons. GMB cultivar have specific polymorphic DNA on the size of 285 bp generated by PMAR primer.Keywords: Cucumis melo L., Gama Melon Basket, genetic variation, RAPD
Struktur Histologis Hepar, Intestinum, dan Ren Burung Cerek Jawa (Charadrius javanicus Chasen 1938) Dengan Kontaminasi DDT di Delta Sungai Progo Yogyakarta Dewi Puspita Sari; Suwarno Hadisusanto; Istriyati I
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.479

Abstract

Residues of DDT (Dichloro Diphenyl Trichloroethane) in the river environment can get into the Bird's body through an intermediary Javan Plover natural feed. Residues of DDT will experience biomagnification and biotransformation in the body organism. The purpose of this research was to determine residue of DDT in the Progo’s mudflat region, several organs of birds, and their impact on liver, intestine, and kidney Javan Plover. This research was located in Progo’s downstream. The method of environment and feed sampling done directly on site. The bird sampling using mist nets. Detection of residues DDT in environment samples by gas chromatography. Measurement of DDT residues in feed and organs of birds with spectrophotometer. Preparation of histological slide from liver, intestine, and kidney using paraffin method (with Hematoxilin & Eosin and Mallory Acid Fuchsin staining). Quantitative Data obtained from the measurement results of environment, feed,and organs of Javan Plover samples. Qualitative data analize by descriptively. The analysis showed DDT residues detected in area’s Progo downstream, natural feed and Javan plover’s organ. Analysis shows chronic effects posed by DDT residue on the histological structure of liver, intestine, and kidney of Javan Plover.Keywords: DDT (Dichloro Diphenyl trichloroethane), histological structure, Javan Plover(Charadrius Javanicus Chasen 1938), Progo’s River
Keragaman Nukleotida Gen Lcy-b (Lycopene beta cyclase) Kultivar Tomat Betavila F1, Fortuna F1 dan Tymoti F1 Muhammad Thoifur Ibnu Fajar; Purnomo P; Niken Satuti Nur Handayani
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 2 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i2.2508

Abstract

Tomato has three lycopene gene, namely lycopene beta cyclase (Lcy-b), lycopene beta-cyclase kromoplas (Cyc-b) and lycopene epsilon cyclase (Lcy-e). Lcy-b gene can be used as determinant of phylogenetic relationship between tomato and chili. This research aims to know phylogenetic relationship among tomatoes cultivar, namely Betavila F1, Fortuna F1 and Tymoti F1, then phylogenetic relationship between these tomatoes cultivar and tomatoes sequences Kristin KC140137.1, Darsirius KC140135.1, Pennellii XM 015217853.1, Villosum KP313876.1 and outgroup Capsicum annuum GU085266.1. Besides that, this research also aims to know the difference of nucleotide among tomatoes cultivar and the others tomato sequences and the outgroup Capsicum annuum based of alignment result. PCR (Polymerase Chain Reaction) method and NCBI primer design used to isolate Lcy-b gene. Acquired sequences were analyzed by Mega 6 and Clustal X softwares. The result of phylogenetic relationship among tomatoes cultivar showed Betavila F1 cultivar closely related to Tymoti F1 cultivar and Fortuna F1 cultivar closely related to Tymoti F1 cultivar. Phylogenetic relationship between tomatoes cultivar and the other tomatoes sequences showed Fortuna F1 cultivar closely related to Darsirius cultivar and varieties Pennellii, Betavila F1 cultivar distantly related to the other tomatoes sequences, and Tymoti F1 cultivar closely related to Kristin cultivar. The difference of nucleotide were found at Pennellii tomato that is 1 nucleotide with transition mutation, found at Villosum tomato that are 12 nucleotides with transition and transversion mutation, and found at Capsicum annuum that are 16 nucleotides with transition and transversion mutation. Phylogenetic relationship using Lcy-b gene expected to be used as a transgenic strategy to modify carotenoid content that improves tomatoes nutrition
Pengaruh Intensitas Cahaya dan Nutrisi Terhadap Pertumbuhan Miselium Pleurotus ostreatus di Tangerang Gervasius Harwin Wangrimen; Ferdian Ferdian; Meyta Valentine; Yashinta Budiyanti; Indah Juwita Sari
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 2 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i2.3878

Abstract

White oyster mushroom (Pleurotus ostreatus) is a type of beneficial mushroom, widely used for consumption, medicinal ingredients, as well as business-oriented cultivation. Nutrition and light intensity are two of the most influential factors for the growth of white oyster mushroom mycelium. Nutrients such as water used for rice cooking (traditionally called air leri) and glucose solution are thought to be potential sources of nutrients, but it is not known which nutrients are more suitable for the growth of white oyster mushroom mycelium. This research was conducted to find out the growth rate difference of white oyster mushroom mycelium produced by vary LED (Light-Emitting Diode) light intensity (sequentially 0 lux, 103 lux, 2x103 lux, 3x103 lux, and 4x103 lux ), air leri nutrition with a 1:1 dilution ratio, glucose solution with 1:3 ratio. Processed data showed that the growth rate of white oyster mushroom mycelium with air leri nutrition for 18 days is better than glucose solution, while low light intensity will affect the color to the growth rate of mycelium on the bag log medium.

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