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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 544 Documents
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The Effect Nitrogen Fertilizer Application on the Resistance of Tobacco (Nicotiana tabacum L. ‘Bligon’) against Drought Stress Rima Vegi Santika; Siti Nurbaiti; Putra, Sidiq Permana; Rachmawati, Diah
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.09

Abstract

Tobacco (Nicotiana tabacum L.) is a plant that has high economic value. Water availability is a challenge in cultivating tobacco plants. Drought can affect plant physiology, reducing the metabolic rate and productivity of tobacco plants. Urea fertilizer contains nitrogen as a macronutrient, which plays a role in vegetative growth and water absorption in drought stress. This research aims to study the resistance response of tobacco to varying conditions of water availability and nitrogen fertilizer application. The water availability treatment provided includes 100%, 75% and 50% of the field capacity. Variations in the dose of nitrogen fertilizer were 0.6 g; 1.2 g; and 1.8 g/polybag. This research was conducted using a Randomized Complete Block Design (RCBD) with three replications. The parameters measured were resistance responses including H?O? content and antioxidant enzyme activity such as SOD, CAT, and APX. The results showed that the more severe the drought, the higher the H?O?  levels. The plant resistance mechanism is indicated by the activity of the antioxidant enzymes SOD, CAT, and APX, despite the fluctuating values observed during the experimental period. The application of nitrogen fertilizer at doses of 0.6 g, 1.2 g, and 1.8 g/polybag showed no significant differences in the physiological responses of the plants. As a result, a dose of 0.6 g/polybag can be recommended as an agronomically and economically efficient.
Assessing the Vulnerability of Mangrove Habitats in the Coastal Area of Biak Timur District, Papua, Indonesia Nifaan, Welmincie Milana; Pattiasina, Thomas Frans; Purba, Gandi Yantri Sevantina; Toha, Abdul Hamid A.; Manuhutu, Jafry Ferdinan; Boli, Paulus; Pattiasina, Selfinus
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.10

Abstract

The vulnerability of mangrove habitats to environmental changes driven by anthropogenic activities and climate change is a critical concern for sustainable ecosystem management. This study assesses the vulnerability of mangrove habitats along the coastal area of Biak Timur District, Papua, using the Coastal Vulnerability Index (CVI) method. The CVI integrates five key variables: coastline profile, duration of tidal inundation, maximum tidal inundation height, salinity, and substrate type. Coastline profile data were derived from a decadal analysis (2013–2023) of shoreline changes. Tidal inundation duration and height were obtained through tidal data analysis, while salinity and substrate data were collected through field surveys and laboratory analyses. Each variable was scored and weighted within the CVI framework. The results indicate that the mangrove habitat in Biak Timur is in the "high vulnerability" category, with a CVI score of 5.69. This suggests a significant susceptibility to degradation from environmental changes, particularly those caused by human activities and climate change. Effective management should focus on minimising anthropogenic impacts and promoting sustainable use of mangrove resources.
Molecular Analysis and Morphological Characterization of Tor sp. using DNA Barcode COI from Telaga Rambut Monte, Blitar, East Java, Indonesia Damayanti, Ayu Elysia; Rabbani, Calista Naya; Sulung, Cahya Ajeng Valenta Tresna; Rusdianto, Rusdianto; Nugroho, Endik Deni; Mamat, Noorhidayah Binti; Rahayu, Dwi Anggorowati
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.011

Abstract

The genus Tor (Cyprinidae) comprises highly diverse freshwater fishes widely distributed in Southeast Asia, including Indonesia. However, information on Tor species in several regions remains limited. This study aimed to identify Tor species from Telaga Rambut Monte, Blitar, using morphological analysis and molecular approaches based on COI DNA barcoding. Fish were collected through purposive sampling at depths of 1–2 m, and fin tissues were preserved in ethanol for DNA extraction. PCR amplification employed LCO1490 and HCO2198 primers, with sequence analysis conducted using BLAST and the BOLD System. Phylogenetic reconstruction applied Neighbour-Joining and Maximum Likelihood methods under the Kimura 2-Parameter model, supported by p-distance analysis with MEGA, Clustal X, BioEdit, and ABGD. Morphological characterisation confirmed the specimens as Tor tambra. COI gene amplification produced a 477 bp sequence, which showed 92% genetic similarity to the reference T. tambra. The phylogenetic tree revealed a distinct clade with a high bootstrap value (96%), clearly separating T. tambra from other congeners. Integrating morphological and molecular data provides robust evidence confirming the presence of T. tambra in Telaga Rambut Monte. This research represents the first study combining genetic identification, morphological analysis, and phylogenetic reconstruction of T. tambra from this locality. The findings contribute to understanding freshwater fish biodiversity in Indonesia and provide important baseline information on the distribution of local fish resources.
A Comparative Profile of Free Radicals, Endogenous Antioxidants, and Cytokines in Mouse Model of Type 1 Diabetes Mellitus Fikriya Novita Sari; Rizky Senna Samoedra; Setyaki Kevin Pratama; Sri Rahayu; Aris Soewondo; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.04

Abstract

Diabetes mellitus is a metabolic disorder characterized by high blood glucose (hyperglycemia). Hyperglycemia will cause the body to undergo physiological changes such as free radical, antioxidant and inflammation alteration. This research aims to compare the profile of free radicals, endogenous antioxidants, and cytokines in mouse models of type 1 diabetes mellitus (T1DM). Mice were separated into two different groups, normal and diabetic mice groups. The normal group was a group of mice that were not induced to have diabetic conditions, while the diabetic mice group was induced to be diabetic using streptozotocin injection. Blood glucose levels were checked every three days for 14 days, while the immune response was evaluated after 14 days using flow cytometry. Data analysis was done using SPSS software with t-test analysis. This research showed that the increasing ROS represented by MDA would trigger inflammation in T1DM represented by the increasing TNF-alpha along with IFN-gamma and reducing anti-inflammatory cytokines represented by IL-10. Interestingly, SOD expression, which is an endogenous antioxidant, is also increased in the diabetic mice group, and we conclude that it is some sort of adaptive response of the diabetic mice group against the increasing ROS.
Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.) Setiani, Nur Asni; Rafika Zahraeni; Siti Uswatun Hasanah
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.01

Abstract

Endophytic bacteria are in symbiosis with plants and have the potential to produce secondary metabolites similar to their host. Kumis kucing (Orthosiphon aristatus) is a traditional medicinal plant that has been proven to have many pharmacological activities, including antiviral and antibacterial. This study aims to isolate and characterize endophytic bacteria from kumis kucing leaves. Endophytic bacteria were isolated from kumis kucing leaf by spreading method on Trypticase Soy Agar (TSA) media and incubated for 24 hours at room temperature. Colonies that grew with bacterial morphology were inoculated on TSA media to obtain pure cultures. Pure cultures of isolates were identified through Gram staining, 16S rRNA gene sequencing, and bacterial growth curves. There were eight isolates with bacterial morphology, which were identified by Gram staining. The staining results showed that all were classified as Gram-negative with rod and coccus shapes. The 16S rRNA gene sequencing identified three bacterial isolates: Acinetobacter schindleri, Pantoea agglomerans, and Pseudomonas lurida. The three bacteria have different time to reach stationary phases in order to produce their secondary metabolites.
Computational Design of a Truncated Javan Spitting Cobra (Naja sputatrix) Venom Cardiotoxin Analogue as a Promising Insulinotropic Agent Dinana, Ichda Arini; Widodo, Widodo; Kurniawan, Nia
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.03

Abstract

Treatment options for diabetes may vary widely, however, around 50% of all diabetic patients do not reach the minimum glycemic target. Therefore, drugs that are able to lower glucose levels through unconventional targets are still needed for the treatment of type 2 diabetes. This study aimed to investigate the activity of cardiotoxins from Naja sputatrix towards Kv channels as potential targets for glucose level reduction. Molecular docking analysis was conducted using the ClusPro web server, and the resulting protein-ligand interactions were visualized using BioVia Discovery Studio and LigPlus v.4.5.3. The stability of the docking structures was further examined through molecular dynamics simulations. The CTX-1, CTX-3, CTX-4, CTX-5, and CTX-KJC3 sequences from N. sputatrix were aligned and modeled, focusing on the 62-81 amino acid residues. The CTX-3, CTX-4, and CTX-5 models demonstrated interactions with the binding site of the KcsA receptor. Additionally, substituting the Val73 residue in the CTX62-81 fragment with Lys73 resulted in reduced binding energy and mitigated the cytotoxic effects of CTX while maintaining its insulinotropic activity.
Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin Nafika, Wirentyas; Munawarti, Aminatun
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.02

Abstract

Apple rootstock is a plant type used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. This study aims to examine the influence of auxin and cytokinin ratios on morphogenesis in leaf and petiole explants of rootstock apples, seeking optimal response modifications. A 2-factorial, completely randomized design (CRD) was utilized in the investigation, with variations in the types of explants and combinations of plant growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin, BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation, respectively. Most calluses have a compact texture with green, yellowish-white, and brownish-green variations. The PGR combination with the highest percentage of callus production in leaf explants was BAP 1 ppm + IBA 0.4 ppm treatment, whereas the petiole was BAP 1 ppm + IBA 0.3 ppm treatment. Therefore, the combination of PGR with the best callus response in this study can be used as a reference in the development of apple tissue culture.
Antioxidant Activity in Melon (Cucumis melo L. 'Gama Melon Parfum') as Antiaging Cream Formulation Wahyuni, Satria; Wibowo, Wiko Arif; Sulaiman, Teuku Nanda Saifullah; Daryono, Budi Setiadi
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.05

Abstract

Antiaging cosmetics are becoming increasingly popular among the general public. Melon (Cucumis melo L.) Gama Melon Parfum cultivars ('GMP') are one of the plants that can be utilized as a basic ingredient in the production of antiaging cosmetics. 'GMP' melon has cucurbitacin compounds that can be utilized as antiaging and contains flavonoid, saponin, and terpenoid compounds that have antioxidant properties. This research aims to analyze the difference in antioxidant activity of 'GMP' melon extract and 'GMP' melon cosmetic cream preparation cultivated in Yogyakarta with 'GMP' melon cultivated in Bandung, analyze extract standardization parameters, and determine the phenolic and flavonoid contents of each melon extract contains. The activity of antioxidants was determined by the DPPH method, the Folin-Ciocelteu method to measure phenolics, and the AlCl3 solution-based colorimetric approach to measuring flavonoid content. It was revealed that the highest % RSA of antioxidant activity is in ‘GMP’ melon extract cultivated in Bandung. The results of the standardization of 'GMP' melon extract met the requirements of standard parameters. The greatest total phenolic content is derived from the extract of ‘GMP’ melon in Bandung, while the highest total flavonoid content in 'GMP' melon extracts is cultivated in Yogyakarta. The highest to lowest antioxidant activity of ‘GMP’ melon cream was formula IV (‘GMP’ melon extract 30% cultivated in Bandung), formula III (‘GMP’ melon extract 20% grown in Bandung), formula II (‘GMP’ melon extract 30% cultivated in Yogyakarta), formula I (‘GMP’ melon extract 20% cultivated in Yogyakarta), and the lowest is in K- with formula without ‘GMP’ melon extract.
The Effect of Storage Time on The Quality of Common Carp Sperm (Cyprinus carpio) Strain Punten in NaCL Solution and Coconut Water (Cocos nucifera L.) Extender Triandari, Ade; Isna Hapsari, Ajeng Hanum; Iskandar, Rossdannisa; Warih Marhendra, Agung Pramana; Soewondo, Aris; Sri Rahayu
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.05

Abstract

Cyprinus carpio is a freshwater species with high reproduction and adaptability. These factors make C. carpio being the freshwater fish that majority distributed and has high commercial value in some countries, including Indonesia. The maturation phase of male and female fish gonads does not occur simultaneously. Thus, sperm preservation could be an alternative technique to maintain the breeding process throughout the year. This research aims to determine the effect of storage time on C. carpio sperm quality strain Punten in NaCl solution-coconut water (Cocos nucifera L.). This study used common carp (C. carpio) 8 months of age with 500 g.bw-1. The C. carpio semen sample was divided into four treatment groups (0, 3, 6, and 9-hour storage time). The extender used in this research was composed of coconut water (C. nucifera L.) and NaCl solution with a ratio of 70% : 30%. The samples were stored in a refrigerator at 5oC. Fresh sperm sample was analyzed macroscopically using various parameters, such as pH, volume, color, and consistency. Meanwhile, the post-preserved sperm quality sample was analyzed microscopically (motility, viability, concentration, and abnormality). Furthermore, we also obtained the fertilization rate and hatching rate. The result showed that storage time affects the reduction of C. carpio sperm quality significantly (p<0.05). The highest sperm viability was obtained in NaCl solution-Cocos nucifera with a 3-hour storage time (78.33%±5.49). Our study found that the storage time significantly affected the sperm quality of C. carpio that was given with C. nucifera extender. Adding C. nucifera with 3,6,9 hour storage time also increases the fertilization and hatching rate compared with the group without C. nucifera. It can be shown that the addition of C. nucifera as a natural extender has the essential role of maintaining C. carpio sperm quality after a short storage period.
Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot Nur Aini; Suharjono
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.03

Abstract

Shallot basal rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation, and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that two bacterial isolates, B7 and B2, had the highest importance value index of 94.21% and 90.59%, respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.

Page 54 of 55 | Total Record : 544

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