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WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
ISSN : 02166461     EISSN : 23546832     DOI : 10.14334
Core Subject : Health,
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences ISSN: 0216-6461 E-ISSN: 2354-6832 is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1983. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Arjuna Subject : -
Articles 785 Documents
Disease Control in Animals Using Molecular Technology by Inactivation of ASO, RNAi and ss-siRNA Genes Muhamad Ali
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 24, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.86 KB) | DOI: 10.14334/wartazoa.v24i1.1024

Abstract

Globalization causes high mobility of human and livestock, hence increase the transmission of infectious diseases, including avian influenza, severe acute respiratory syndrome (SARS), and swine influenza. Therefore, prevention of those diseases is required. Vaccines are effective to prevent infectious diseases; however, their development takes a long time and they cannot provide immediate protection in pandemic cases. This paper describes several gene silencing technologies including antisense oligonucleotide (ASO), RNA interference (RNAi) and single strand-small interfering RNA (ss-siRNA) for controlling diseases. The primary mechanism of these technologies is inhibition of gene expression, typically by causing the destruction of specific RNA molecule of the pathogen. The use of gene silencing technologies is expected to give new alternative that is more effective in eradication of infectious diseases in animals before threaten human being. Key words: Gene inactivation, antisense oligonucleotide, RNA interference, single strand-small interfering RNA
Aflatoxin in Animal Feed in Indonesia: The Regulation on The Toxic Content and The Development of Detection Technique Sri Rachmawati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1062.095 KB) | DOI: 10.14334/wartazoa.v15i1.835

Abstract

Aflatoxin contamination of agricultural commodities including feedstuff potentially occurs in Indonesia, and can cause problem to animal health and productivity . To minimize the impact of such contamination to both human and animal health, regulations regarding feed quality have been issued by the government . A continuous monitoring of the contamination using a simple, sensitive, rapid and cost-effective method is greatly needed. This paper contains some information about the current situation of aflatoxin contamination in feed and its ingredient (corn), the regulation related to aflatoxin contamination and the development of detection technique for analysis aflatoxin B, in feedstuffconducted by Research Institute for Veterinary Science (RIVS). From research results, inter laboratory studies and collaboration research conducted in Indonesia, they indicate that poultry feed collected from different areas of Indonesia is contaminated by aflatoxins . Results of inter laboratory study and collaborative research with Feed Lab, Directorate General for Livestock Services (DGLS) showed that 14 .0% of 207 feed samples from different sources contain aflatoxin above the standard determined by Standar Nasional Indonesia (SNI) . Regulations related to aflatoxin content in feed are compiled in the SNI (revised formed), the Minimum Technical Requirement of feed concentrate for ruminant and non ruminant, and the regulation from DGLS, letter No 524/TN .250/Kpts/DJP/Deptan/1997. RIVS developed an ELISA technique for analysis aflatoxin in feed and corn, which was involving some steps of activities include hapten synthesis, production and characterization of antibody, development of assay performance, designing a method as a prototype kit . For validation of this technique, RIVS has conducted a training workshop and a field trial . Rapid assay ELISA kit has been designed with incubation time of 5 minutes for conjugate and 10 minutes for substrate . Antibody response was specific to AFB, (100%) with cross reactivity of 0 .9, 3 .5 and 1 .6% for aflatoxins B,, G, and G,, respectively . The limit of detection of AFB, was 0 .3 ppb . The range of analysis is from 0 .3 ppb up to 30 ppb . An ELISA kit composed of seven bottles of AFB, standards solution of 30 . 10, 3 .3, 1 .2, 0 .4, and 0 .12 ppb and blank (0 ppb AFB,), a conjugate of AFB I-HRPO, a substrate, a stopping solution, an antibody coated plate, and one mixing plate . The kit was stable at 4 °C for two months . The field trial of ELISA kit showed an accurate result and when comparing the ELISA method with the standard method of HPLC, a consistent result was also found . With the information given above, people or farmers should be aware of the danger of aflatoxin and should take precaution to prevent aflatoxin contamination . RIVS ELISA kit is a useful technique to detect aflatoxin in feedstuff and, hence, controlling the aflatoxin contamination . Keywords: Aflatoxins, ELISA, detection, feed
Cadmium (CD) in The Environment and Its Effect on The Animal Health and Productivity Darmono .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 8, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.684 KB) | DOI: 10.14334/wartazoa.v8i1.739

Abstract

Cadmium (Cd) is a nonesential element which often cause of environmental pollution and toxic effect to the animal and man. Cadmium also has an acummulative effect in the tissue of biological life, as a consequence it cause contamination in feed and food such as grass, grain and animal tissue. Because ofthat Cd enter into food chain which consumed by animal or human. In the animal Cd can cause of tissue damage and produced acute or chronic toxicity, as consequence cause of disease and decrease of animal production. Key words : Cadmium, environment, animals
Brucellosis: An Unrecognized Zoonotic Disease in Indonesia Susan Maphilindawati Noor
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.624 KB) | DOI: 10.14334/wartazoa.v16i1.867

Abstract

Brucellosis, a bacterial zoonosis, is a disease caused by members of the genus Brucella . In Indonesia, brucellosis has known as a contagious reproductive disease in animals, however, only certain people know that brucellosis can be transmitted to human . Bovine brucellosis is characterised by one or more of the following signs : abortion, retained placenta . orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk . The Brucella organism is transmitted to human most commonly by ingestion of untreated milk or milk products or through the mucous membranes and wound of the skin . The severity of human disease varies, depending largely upon the infecting strain . Brucella abortus, B . melitensis. B . suis and B. canis are highly pathogenic for humans . Clinical symptoms of human brucellosis may include an intermittent fever, headaches, weakness, arthalgia, myalgia and weight loss . Occasional complications include arthritis, endocarditis, hepatitis granuloma, meningitis, orchitis dan osteomyelitis have also been reported . Brucellosis can also produce spontaneous abortion in pregnant woman . Diagnosis is based on the isolation of the organism and serology . Antibiotics are usually the mainstay of treatment and long-term treatment may be required . Brucellosis can be controlled by comprehensive campaigns to eradicate the disease by vaccination programme followed by test and slaughter of domestic animals which exhibit positive serologic reactions to brucellae.   Key words : Brucellosis, zoonosis, diagnose, control
Crop-Livestock Integration in a Competitive, Sustainable and Democratize Agribusiness Development Kusuma Diwyanto; Bambang R Prawiradiputra; Darwinsyah Lubis
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 1 (2002)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.267 KB) | DOI: 10.14334/wartazoa.v12i1.771

Abstract

The concept of integrated crop and livestock in Indonesian farming systems has actually been practiced by farmers since they familiar with farming, but they practiced  the systems by traditional way, without any consideration of cost-benefit as well as environmental impact. The research on crops-livestock systems has been conducted systematically since early 1980’s. The study considered many aspects in sustainability such as environmentally tolerable, socially acceptable, economically feasible and politically desirable. In late 1990’s the crops-livestock systems came to a very important step with intensifying the integration between rice and cattle, in this case through optimization of the manure. The added value of organic fertilizer could reach till 40%. In dairy cattle such a technology was able to benefit Rp 11.000 per cattle per day. In this case, with LEISA (low external input sustainable agriculture) approach, any 8 − 10 liter of milk produced by  a head of cattle needed feed equal to as much as 3 −4 liter of milk only. With LEISA approach the crops-livestock systems has empirically proved the ability of providing job by using the local input efficiently. In improving efficiency, the use of local resources such as local feed and local cattle need to be optimized. To develop the technology that support the concept of crop-livestock systems, the Government could help the farmers through extension so that the farmers willing to use organic fertilizer, especially manure of the cattle. The rice field in the northern part of West Java is a huge potency to produce rice straw as cattle feed. Based on the fact, the beef cattle can be developed in this area so that the manure available to fertilize the rice fields. If the crop-livestock systems is successfully developed in the area, the improve of rice production and productivity is happened, and in turn, the farmers welfare will also increase.   Key words: Farming systems, crops, livestock, organic fertilizer, local resources, crop by product
Environmental Awareness on Beef Cattle Technology Abdullah M Bamualim; A Thalib; Y N Anggraeni; Mariyono .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.268 KB) | DOI: 10.14334/wartazoa.v18i3.899

Abstract

The acceleration program to meet beef self sufficient in 2010 is expected to increase animal protein consumption of Indonesian people in order to be equal with other countries as well as to improve the livestock farmer’s income. The main objective of the program is to increase cattle population. Since the availability of forage and grassland is limited, beef cattle development is driven to the crop and plantation integration approach by using their by-product as cattle feed. Crop and plantation by-products, generally are considered to be fiber source with high lignocellulose’s and low nutritive value. Feeding high fiber would increase methane gas production, and faeces and grass cultivation also contributed on greenhouse emission. Methane is one of the main greenhouse gases contributed by agriculture sector; increasing beef cattle population using high fiber feed is predicted to increase methane production. Good management is expected to improve productivity and to reduce methane production on livestock. Some efforts could be done such as good feeding management and nutrition manipulation, environment friendly  cattle  waste  management,  improving  management on  roughage  cultivation,  and  improving  management  on  cattle production.   Key words: Beef cattle, agricultural by product, methane, global warming
Cauda Epididymis Spermatozoa: Cryopreservation and Utilization for Artificial Insemination and In Vitro Fertilization Fitra Aji Pamungkas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 22, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2565.871 KB) | DOI: 10.14334/wartazoa.v22i4.967

Abstract

Genetic material either from animals of economical interest or from wildlife conservation can be lost anytime by unexpected death of the animal, low libido, or disorder at reproduction. In this case, an effort can be made occur to avoid the total lost of that genetic material by using an epididymis spermatozoa. Cauda epididymis spermatozoa generally motile, mature and can be used to fertilize oocytes as well as ejaculated spermatozoa. Some research indicated that cryopreservation of cauda epididymis spermatozoa for the purpose of artificial insemination and in vitro fertilization showed the ability to fertilize oocytes and produce offspring. Key words: Spermatozoa, cauda epididymis, artificial insemination, in vitro fertilization
Impacts of Verotoxigenic and Enterohaemorrhagic Escherichia Coli (Vtec and Ehec) on Animal, Human and Food Widodo Suwito
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.554 KB) | DOI: 10.14334/wartazoa.v19i2.922

Abstract

Verotoxigenic and enterohaemorrhagic Escherichia coli, VTEC and EHEC are strains that produce a toxin on the Vero cells in vitro, therefore the toxin called verotoxin. Strain VTEC and EHEC have been isolated from human and various animal species, mainly ruminants and pigs. The prevalence of VTEC in cattle is 35%, milk 10%, cheese 1.5%. In beef meat, pork, poultry, goat and sheep is 3.7, 1.5, 1.5, 2 and 2.5%, respectively. In contrast, the human prevalence in United States, Spain and France 5 – 10, 2.5 and 3.4%, respectively. In Indonesia, nine cases of VTEC were reported by Ciptomangunkusumo Hospital and four out of nine cases were died. The verotoxin also named Shiga toxins (Stx), is active in vivo as a pathogen on the endothelial cells of the blood vessels of the gastro intestinal mucosa, kidneys, brain and other tissues of human and piglets. Verotoxin in human, ruminants and piglets causes bloody diarrhea so with ruminants. In the adult ruminants, verotoxin results in clinical signs and they act as asymptomatic carriers of VTEC and EHEC strains but in human, it produced haemorrhagic colitis (HC), haemolytic uremic syndrome (HUS) and thrombocytopenia purpura (TPP). Infection in human by the most famous EHEC strain belongs to the O157:H7 serotype, through faecal contamination or environment of either food of animal origin, or other foodstuffs (fruits and vegetables). Infection from EHEC strains could be prevented by avoiding food of animal origin and unpasteurized milk, and by taking care of food hygiene for comsumption.   Key words: verotoxin, enterohaemorrhagic, VTEC, EHEC, Shiga toxin (stx), ruminant
Organic Zinc as Feed Additive for Ruminants Suprijati .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.753 KB) | DOI: 10.14334/wartazoa.v23i3.1005

Abstract

Zinc is an essential micro mineral required by ruminants and is a component of over 300 enzymes which play important role in the metabolisms of carbohydrates, proteins and fats. Recently, the chemical and biotechnology processes have been developed for synthesizing organic Zn. Organic Zn is the product of a chelating process of dissolved Zn anorganic salts with amino acids or hydrolyzed protein. The utilization of organic Zn as feed additive in ruminants diets tends to increase, due to the absorption  of Zn  in  the  organic  form is  more  effective  than  the  inorganic  form.  This  paper  reviewes  the  research  from comparative studies of inorganic and organic Zn on the growth, production and reproduction in ruminants. The studies found that the supplementation of organic Zn improved nutrient digestibility, growth, feed efficiency, milk production, Zn retention and absorption, production and reproductive performances of ruminants. However, a little variation response was found due to different degrees of chelating on Zn incorporation of amino acids or hydrolyzed protein.   Key words: Organic Zn, characteristic, supplementation, performance, ruminants
Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control Sutiastuti Wahyuwardani; Tatty Syafriati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (956.851 KB) | DOI: 10.14334/wartazoa.v15i3.826

Abstract

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, control

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