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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 685 Documents
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Isolation and identification of bacterial protease enzyme of leather waste Pertiwiningrum, A.; Anggraini, F. D.; Fitrianto, N. A.; Rochijan, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.1.33-41

Abstract

The objectives of this study were to isolate and identify bacteria which produced protease enzyme from liquid and solid waste of tannery, and their characterization of enzymatic activities. The bacterial isolation used a sample of liquid and solid waste from leather waste which taken from a different waste reservoirs (three liquid waste and one solid waste in unhairing phase). Data of the isolated bacteria, OD 600 nm bacterial growth, the identification of bacteria, and enzyme precipitation with 60% ammonium sulfate were analyzed descriptively. The colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature were analyzed using Completely Randomized Design, followed by Duncan’s New Multiple Range Test. The second sample from four samples of the isolated bacteria was tested further for their proteolytic activity. The morphology of the colony was circle, white, flat ledges and convex elevation, the basal cell morphology was red, gram-negative, non-motile, catalase positive and gelatin negative. The highest activity of enzyme on pH 11 with activity unit enzyme 45,18±1,77 U/ml and specific enzyme activity 43,19±1,69 U/mg and temperature of 40°C activity unit enzyme 54,02±1,89 U/ml and specific enzyme activity 51,65±1,8 U/mg. The activity of enzyme from protease were precipitated ammonium sulfate 60% showed a higher result of (75,8 U/ml) rather than rough protease.
AMMONIA INHIBITION IN THERMOPHILIC ANAEROBIC DIGESTION OF DAIRY CATTLE MANURE Sutaryo, S.; Ward, A. J.; Moller, H. B.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 2 (2014)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.2.83-90

Abstract

This study investigated the effect of five different concentrations of total ammonia nitrogen (TAN)and free ammonia (FA) on the methane yield and digester performance under thermophilic conditions(50°C). Five identical continuously fed digesters were used. The experiment was run for four timeshydraulic retention time (HRT). Data were statitically analysed using the data Analysis Tool Packavailable with the Microsoft Excel program. Different ammonia levels were obtained by pulsing urea toobtain the target level of TAN and FA, and to subsequently maintaining the concentration of ammonialevels by daily urea additions. The result showed a strong negative correlation between both TAN andFA concentrations and methane yield. The methane yield was reduced by 24, 30, 52 and 66% indigesters that had TAN levels of 2.9, 3.6, 4.4 and 5.1 g/L, respectively, corresponding to 0.7, 1.1,1.5 and1.8 g/L FA. Total volatile fatty acid and especially isovaleric and isobutyric acid concentrations wereelevated during ammonia inhibition. Concentration of TAN in the biogas digester exceeding 3 g/Lindicating that very often the full biogas potential is not achieved due to ammonia inhibition and thatlonger HRT should be used to compensate or a lower process temperature should be chosen.
THE EFFECT OF DIFFERENT MEAT SHOP ON MEAT PHYSICAL CHARACTERISTICS AND BACTERIA POPULATION Dewi, S.H.C.; Prihharsanti, A.H.T.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.59-63

Abstract

An experiment was conducted to study the effect of different meat shops on meat physicalcharacteristics and bacteria population. Sixteen PO carcasses were used in the experiment which wasarranged in a completely randomized design with 4 treatments of different meat shops (traditionalmarket, meat shop, supermarket and slaughter house). Parameters measured were meat pH, waterholding capacity, cooking loss and bacterial total count. The result showed that the average of pH was5.25- 6.03; water holding capacity was 17.07-38.87%; cooking loss was 33.15-48.20 and bacterial totalcount was 1.48x106-10.75x106 CFU/g. It was concluded that bacterial total count in slaughter house andspecial market (meat shop and supermarket) were less than those in traditional market.
Effect of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chickens Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Nurwantoro, N.; Al-Baarri, A. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.286-294

Abstract

The objective of the present study was to evaluate the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks. A number of 288 day-old-chicks (Lohmann MB-202 meat broilers) were distributed to four groups, including CONT (basal diet with no additive as a control), FORM (basal diet containing 0.1% formic acid), BUTR (basal diet with 0.03% butyric acid) and FORM+BUTR (basal diet with 0.1% formic and 0.03% butyric acid). At day 35 the birds were slaughtered and eviscerated, and from which the breast meat was obtained. Carcass and cut yields of broilers were not different (P>0.05) among CONT, FORM and BUTR birds. The combination of formic and butyric acids increased (P<0.05) the relative weight of back of broilers. Meat pH value was higher (P<0.05) in organic acids treated than in non-treated birds. Organic acids lowered (P<0.05) drip loss of broiler meat. Butyric acid increased (P<0.05) moisture and decreased (P<0.05) the content of meat protein. Formic acid increased (P<0.05), whereas butyric acid and combination of formic and butyric acids decreased (P<0.05) lightness values of breast meat. Yellowness values of meat increased (P<0.05) with organic acid administration. Intense bands of 25 kDa (phosphoglycerate mutase) was observed in most of meat samples from the treated birds, but not in meat from the control birds. In conclusion, dietary supplementation of organic acids was capable of improving the meat quality of broiler meats. The treatments may prevent the pale, soft and exudative (PSE)-like condition in broiler meats. 
Comparative performance of Landrace, Yorkshire and Duroc breeds of swine Zebua, C. K. N.; Muladno, M.; Siagian, P. H.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.147-152

Abstract

The objectives of this study was to analyze the comparative ability of animal genetic, using evaluation of production that has important economic traits including Landrace, Yorkshire, and Duroc breeds of swine. A total of 49 swine consist 15 boars and 34 gilts, with average body weight of 18.50-21.50kg were used in this research. All of samples were raised in the same pen and feeding management. This research used the experimental method of Completely Randomized Design involving three treatments of animal breeds including Landrace, Yorkshire, and Duroc breeds. The data were analyzed by using analysis of covariance procedur General Linier Model and Least Square Means test. Boars and gilts were analyzed in separated data. The result showed that the effect of breed was significantly associated for all traits, but not for boars feed intake. The overall, Yorkshire and Landrace performed better than Duroc for average daily gain, feed conversion ratio, selection index, and age at 90 kg body weight. However, Duroc boars and gilts had the thinnest backfat followed by Yorkshire and Landrace.
GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON MITOCHONDRIAL DNA D-LOOP SEQUENCE M. F. Harlistyo; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.204-209

Abstract

This study was aimed to find out the diversity of mtDNA D-loop at Kejobong goat. The completemtDNA D-loop sequence of 12 goat blood samples were analyzed from 4 different location inPurbalingga Regency, Central Java province, sub-districts Kejobong, Pangadegan, Bukateja, andKaligondang. The mtDNA D-loop was extracted from blood sample. DNA obtained were amplified byPCR (Polymerase Chain Reaction) method using primers (5’-tcactatcagcacccaaagc-3’) as forward and(5’-ggcattttcagtgccttgct-3’) as reverse and subsequently sequenced. After nucleotide sequencing analysisconducted, 548 bp along was obtained. Nucleotides were then aligned with Capra hircus (GenBankAccess No.: KF952601.1) and apparently there were 11 different sites on the segment of mtDNA Dloop.Five sites could be used as a specific marker to distinguish between the Capra hircus andKejobong goat, namely at the site of 317 (A-G), 403 (T-C), 434 (T-C), 537 (C-T), and 553 (A-G).Nucleotide sequence analysis also contained seven different haplotypes. It was concluded that thedistribution of the different sites showed different haplotype patterns in Kejobong goat.
ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG) Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.; Fathul, F.; Adiati, U.; Praira, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.159-165

Abstract

The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detectPAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT),DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as aplacebo. The 0.5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume withcomplete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected atmutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginalear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measuredusing Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immuneresponse among others and also could differenciate ovPAG in the urine of pregnant ewes It could beconcluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG atmolecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as amajor protein for producing antibodi.
Implication of feed restriction during growth period on the growth hormone profiles and morphology ovary of quail hen (Coturnix coturnix japonica) Hertamawati, R.T.; Soedjarwo, E.; Sjofjan, O.; Suyadi, S
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.415-422

Abstract

The aim of this research was to evaluate the effect of feed restriction on growth hormone profiles and ovarian morphology during the growth period. Three hundred 14-day-old quails were used. The quails were maintained on two feeding restriction programs: two dietary regimes based on metabolizable energy (ME), R1 = 2900 kcal/kg and R2 = 2800 kcal/kg, and 3 quantitative feed restriction diets, P0 = 100% ad libitum; P1= 90% ad libitum and P2 = 80%ad libitum (n=300). Each group (n=50) was processed with five replications, 10 birds in each replicate. The change in growth hormone was determined at 28, 35, 42, and 49 days of age, while ovarian morphology was determined at sexual maturity. The results indicated that feed restriction induced a significant increase in growth hormone and insulin-like growth factor-1 after re-feeding. There was no significant effect caused by the rationing of metabolizable energy. The number of large yellow follicles was not different between quails fed with 100% ad libitum and 90% ad libitum. However, feed restriction significantly increased the number of small yellow follicles. In conclusion, the feed could be restricted to 90% ad libitum with a 2900 kcal/kg ration of ME and fed during the starter period (14 to 42 days of age) without influencing ovarian morphology in quails.
The optimation of farmers families' revenue the integration of Pasundan cattle and paddy farming in West Java Tawaf, R.; Paturochman, M.; Herlina, L.; Sulistyati, M.; Fitriani, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.270-278

Abstract

This research aimed to analyze the revenue optimization of farmer family with ratio of the most ideal farm scale in the integration of Pasundan cattle and paddy farm, and to analyze the most leveraging production factor towards the family revenue. The respondents were 94 farmers who had integrated farming between cattle and paddy. This research used survey method in four regions purposively based on Pasundan cattle centre. Data were analyzed with linear programming and production function of Cobb Douglass. The results were: (1) max Z = -(4,584,841x1+1574260 (x2+x3)) + (6,000,000jx1+4000000 (j x2 +j x3 )); this function consist of constraints: labour, transfer products between cattle and paddy farm, transfer fertilizer, capital of paddy farming in planting season-I and season-II. (2) Yintegration = 15,721,319.75 – 72.541 land – 1.317 fertilizer + 4.667 seeds + 487765.94 farminglabour + 6339170.199 cattlevalue – 935.559 feed + 162618.999 cattlelabour. There were some conclusions: First, the integration of Pasundan cattle and paddy farming produced the optimum family revenue/year, with ratio of 6.02 animal unit and 0.5 ha of paddy farming; Second, the production factor of agricultural land, labour, seeds, feed and capital (cattle) were explained (R2=87.66%) toward the integration revenue; Third, the variable which has the contribution to leverage the revenue was the capital of cattle (81.52%).
LEVEL OF ESTROGEN HORMONE AND ESTRUS PERFORMANCE OF DIFFERENT POSTPARTUM ESTRUS OF JAWA RANDU GOAT Tanjung, A. D.; Setiatin, E. T.; Samsudewa, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.87-92

Abstract

The purpose of this research was to determine the reproductive quality of normal compared withabnormal postpartum estrus (PPE) of Jawa Randu Goats (n = 16 goats) on estrogen hormon level andestrus profile. Total samples were 16 Jawa Randu goats. The data were analyzed descriptively and nonparametric data were analyzed using Mann Whitney U-test of Statistical Package for the Social science(SPSS) 16. Descriptive statistics was used on estrogen hormone and resulting that normal postpartumestrus (PPE) Jawa Randu Goats had a better level (133.8; 141.5; 155.6; 162.25; 167.75 pg/mL)compared with abnormal postpartum estrus of Jawa Randu Goat (109.9; 111.35; 101.2; 132.2; 142.45pg/mL). Mann Whitney U-test used for the abundance of cervical mucus and ferning showed nonsignificantly different of postpartum estrus between both groups. Jawa Randu goats with normal PPEhad a higher estrogen level than abnormal PPE goats. However, for the abundance and ferning ofcervical mucus, between the two groups of goats was not different.

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