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Awalul Fatiqin
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Biota
Published by Universitas Islam Negeri Raden Fatah Palembang
ISSN : 2528262X     EISSN : 24607746     DOI : https://doi.org/10.19109/Biota
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology
Jurnal biota is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biology, with particular attention paid to the exploration and biodiversity Wetland tropical—and especially Indonesian—biodiversity. Original research articles written in English and featuring well-designed studies with clearly analyzed and logically interpreted results are accepted, with a strong preference given to research that has the potential to make significant contributions to both the field of biology and society in general
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 160 Documents
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Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck) Septarini Dian Anitasari; Ida Ayu Astarini; Made Ria Defiani; Made Pharmawati .; Dian Catur Prayantini
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3340

Abstract

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important. The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture. The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore. Pollen viability was determined by IKI staining and pollen germination method. Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development. Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture. Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’.
Daily Activity of Male Mice (Mus musculus) in Laboratory Rian Oktiansyah
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3359

Abstract

The importance of observing the behavior of mice in the laboratory is additional information to compare with their behavior in nature and an effort to increase the welfare of mice (animal welfare) through alleviating the pain and suffering associated with scientific procedures. This study aims to study the daily activities of male mice in the laboratory. The method used was ad libitum and focal animal sampling. Based on the ad libitum sampling method, the daily activities of male mice in the laboratory were divided into 9 activities, namely locomotion, grooming, eating, foraging, social, exploration, resting, making nests, and drinking. Based on the focal animal sampling method, the main activity carried out by male mice in the laboratory was locomotion.
Identification of Anticancer Active Compound from GC-MS Test Results of Zodia Leaves (Evodia suaveolens) Ethanol Extract Ghea Dionita Sanora; Elly Yana Mastura; Muhammad Ongky Muji Handoyo; Erlix Rakhmad Purnama
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3374

Abstract

Cancer is estimated to be the second leading cause of death globally in 2018. The background of the study was the prevalence of cancer in Indonesia in 2018 reaching 775.120 people. Treatments such as surgery, chemotherapy, radiotherapy, immunotherapy, and hormone therapy have negative side effects. Herbal medicine is an alternative treatment with lower side effects that can reduce the side effects of existing treatments. The aim of this study was to identify the results of GC-MS active compounds from ethanol extract of zodia leaves which have the potential as anticancer. The method of this research was observational research through scientific literature. GC-MS results showed 22 active compounds contained in ethanol extract of zodia leaves. Based on the literature searching, there are 17 active compounds that have potential to be anticancer. Menthofuran; evodone; Bicyclopentene; Delta-Selinene; 3,5,7-Octatrien-2-Ol, 2,6-Dimethyl; Aromadendrene oxide 2; Alpha-bisabolol; 2,4-Dimethyl-2,4-Heptadienal; Phytol; Squalene; Beta-Tocopherol; D-alpha-Tocopherol; Stigmasta-5,23-dien-3.beta.-ol; (23S)-ethylcholest-5-en-3.beta.ol; 24,25-Dihydrolanosterol; Lanosterol; and Obtusifoliol. The compounds are targeted in breast, lung, and ovarian cancer.
Density and nutrient content of Terebralia pallustris mangrove snails in mangrove ecosystems in Pannikiang Island, Barru Regency, South Sulawesi Sharifuddin Bin Andy Omar; Andi Nur Samsi
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.3798

Abstract

Mangrove snail, Terebralia palustris Linnnaeus 1967, was also found on Pannikiang Island, Barru Regency, South Sulawesi. This study aims to describe the fluctuation of T. palustris density in a year of observation. This research was conducted for ten months from August 2018 to July 2019. There were two research stations. Each station has a large plot measuring 10m x 10m and in it, five small plots are measuring 1m x 1m. Measurements of environmental parameters such as temperature, salinity, and pH were carried out in situ. This snail meat as much as 100gr also analyzed proximate. The proximate analysis of the snail shows that crude protein content is 18.73% so that the snail is very suitable to be used as a source of protein. The highest density is in July 2019 and the lowest density is in August 2018.
Antibacterial Activity Test of Wet and Dried Extracts of Calabash Tree (Crescentia cujete L.) against Aeromonas hydrophilla: Natural drug use extracts of calabash tree to control the growth of Aeromonas hydrophilla by In vitro Noorkomala Sari; Nengah Dwianita Kuswytasari; Awik Puji Dyah Nurhayati
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.3954

Abstract

Study to determine the antibacterial activity of wet and dry extract of the leaf, fruit, and bark of Calabash tree (Crescentia cujete L.) against the growth of Aeromonas hydrophila. The solvent extraction process was done by using 96% ethanol in the maceration method. Antibacterial test results using diffusion agar to decide clear zone and tube series of dilution test to provide MIC and MBC. Fresh leaf extract produces the highest clear zone diameter (20.06 mm), after which fresh bark extract (12.81 mm), and the last is fresh fruit extract (3.22 mm). In contrast to fresh extracts, the dried extracts are have not clear zone. MIC (Minimum Inhibitory Concentration) of Calabash Tree fresh leaf extract against Aeromonas hydrophila is 80%, and MBC (Minimum Bactericidal Concentration) is 100%.
Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus Arkan Setiaji
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.4184

Abstract

Bioactive compounds of plant cell culture extracts are currently being looked at for its potential for the development of nutraceutical products. Tomato callus is known to have cytoprotective activity. The availability for explants and its callus production are two important things. This paper reports the effect of different strengths of medium on seed germination and seedling growth of tomato cv. Permata and sucrose effects on their biomass callus. Seeds were grown on 4 kind of germination media. Hypocotyl were taken as explants for callus induction in MS medium supplemented by 2.0 mg/l BA and 0.2 mg/l NAA, 3% sucrose, and subcultured after 20 days on medium with 6 variations of sucrose. The highest values ​​for tomato hypocotyl’s height were obtained from the ½ and ¼ MS, while the highest fresh weight was obtained from the MS 0. Both the fresh weight and dry weight of the callus increased along with increasing sucrose concentration. The explant age and biomass need to be a concern regarding what kind of callus that will be produced. Application of 5% sucrose concentration is recommended for callus production in this study.
The Influence Of Kesambi Leaf Extract (Schleichera oleosa) On Proliferation Granulosa Cell Goat Ovarium (Caprus aegagrus HIRCUS) In Vitro Lil Hanifah
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.4372

Abstract

Kesambi (Schleichera oleosa) belonging to the Sapindaceae family has very important phytochemical elements including terpenoids, falvonoids, phenolic acids, betulins, betulin acids and others, so that they have enormous benefits in the process of antimicrobials, antioxidants, anticancer and can be used for the production of biodiesel. One of the potency of antioxidant ability as an antioxidant is due to its phenolic compounds. The presence of antioxidant activity in cells can affect the defense of cell protein membranes, so that the cell's ability to carry out division is optimal. In vitro this is mostly done by adding hormones to culture cell media or natural compound substances to increase cell proliferation. This study is an experimental study using a Complete Random Design (CRD) with 5 treatments and 4 replications as follows: K0: granulosa cells and culture media without treatment (0 µl), P1: granulosa cells and culture media which were given 1% porch leaf extract (30 µl), P2: granulosa cells and culture media which were given kesambi leaf extract 1.5% (45 µl), P3: granulosa cells and culture media were given 2% (60 µl) kesambi leaf extract and P4: granulosa cells and culture media were given a 2.5% (75 µl) kesambi leaf extract. In this study, there was an influence on the administration of kesambi (Schleichera oleosa) leaf extract to the proliferation of goat ovarian granulosa cells in vitro in the P3 (2%) treatment ie a dose of 60 µl. However, it did not have a significant effect on the viability of goat ovarian granulosa cells.
Variation Of Andrographolite Conten From Sambiloto (Andrographis paniculata Ness) Which Grow In Various Regions WAHYU JOKOPRIYAMBODO
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.4882

Abstract

Sambiloto is used as a hepatoprotector, enhancing the immune system, antidiabetic, anti-inflammatory, antiomalaria, antidiarrheal and many more. One of the main ingredients of the bitter plant is andrographolite. This plant is widely spread throughout Indonesia ranging from lowlands to high plains with various types of soil and altitude. The existence of this fairly wide distribution can give effect to the content of andrographolite, so that in this study will be studied about variations in andrographolite levels source from various regions. The results of this study will be used as a quality reference for treatment based on andrographolite. Samples of bitter plants are taken from various regions. Furthermore, parts of plants that are above the ground are separated between leaves, twigs and stems. All samples were dried in an oven at 60 degrees Celsius. Pollinated samples were then sieved. Samples were extracted with ethanol overnight and then analyzed their andrographolite levels with TLC-densitometry.The results of the analysis of andrographolite levels showed that bitter from various regions have different levels. Tthe organs of plants also show different levels of andrographolite as well. Part of the leaf organ is the part that contains the most andrographolite.
Inventarisasi Siput Darat di Danau Darungan, Taman Nasional Bromo Tengger Semeru 2019 Putri Afin Nurhayati; Boni Hermawan; Galuh Ayu Chantika Dwitara
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.5219

Abstract

This study aims to determine the diversity of land snails that have never been revealed before. The location of this research is in the forest in the Ranu Darungan Resort area of Bromo Tengger Semeru National Park. The method used in this study is the 1.5 km transect line method. Land snails are often found behind leaves, around tree roots, and around leaf litter. The results of this study obtained 20 species of land snails, each of which has different characteristics. Land snail identification is using a land snail identification book. The lack of information about land snails in the Ranu Darungan Resort area makes this research expected to be the basis for further research.
The Effect Of Colchicine on Phenotypes And Stomata Pakcoy (Brassica Rapa L) Hydroponically With The NFT (Nutrient Film Technique) System nurdiah hasana
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.5375

Abstract

Colchicine (C22H25O6N) is a toxic alkaloid compound that causing a polyploidy in the plants, so it produces a robust individuals. The aims of this research are to know the effect of kolchhisin against phenotypes and stomata pakcoy (Brassica rapa L) hydroponically with the NFT (Nutrient Film Technique) system. This research used experimental method with a complete random design (CRD), five treatments and five replications. The data were analyzed by using ANAVA with 5% of credibility. The sample of this research were used pakcoy red arrow F1 nauli with colchicine P0 (Control), P1 (250 mg / L), P2 (500 mg / L), P3 (750 mg / L), P4 (1000 mg / L). The result of this study showed that the influence of colchisin against phenotype and the stomata’s size of pakcoy plant (Brassica rapa L). The high concentrations of colchicine can caused the size of the phenotype (leaves’ number, leaf area, wet weight, dry weight) pakcoy smaller than the control. While the size of the stomata increased, the largest of stomata size was found in the P3 treatment (750 mg / L).

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