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UNEJ e-Proceeding
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Articles 1,005 Documents
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RESULT OF SEVERAL CLONE OF SWEET POTATO (Ipomoea batatas L.) IN MIDDLE EAST ON THE INFLUENCE OF PHOSFOR FERTILIZER DOSAGE AND CLONE TYPES Mardhiah Hayati; Rita Hayati
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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Sweet potatoes include commodities as the main source of carbohydrates that occupy thefifth position after rice, cassava, wheat and corn. These plants can be cultivated at variousaltitude places in the lowlands, middle and highlands. Judging from the nutritional value ofsweet potatoes have the privilege as an important source of carbohydrates so that thiscommodity becomes an alternative for food diversification program. The purpose of thisstudy is to determine the effect of phosphorus fertilizer dosage and clone type andinteraction between the two treatments on sweet potato yield in the middle plains. Thisresearch was conducted at Suka Makmur Village Farmer Kecamatan Lembah Selawah Districtof Aceh Besar Saree, at an altitude of 560 m above sea level from February to June 2016. Thisstudy used a Randomized Block 3 factorial factorial design with 3 replications consisting of 2Factor, ie treatment of phosphorus fertilizer dosage and clone type. The phosphorusphosphorus treatment (SP 36) consisted of 3 levels, 100, 200 and 300 kg / ha and clone typefactor ie CIP BDG, Local Saree Krem, and Antin 1. The variables observed in this study werewet bulb weight, Dry tuber weight, number of tubers, general evaluation of tubers and tuberproduction per hectare. The results showed that treatment of SP 36 fertilizer dosage was notsignificant on wet bulb weight, tuber weight, tuber amount, general evaluation of tubers andtuber production per hectare. The dose of SP 36 fertilizer tends to be better at a dose of 200kg / ha. The clone type had a significant effect on the number of tubers and significantlyaffected the wet bulb weight, the weight of tubers and tuber yield per hectare and did notsignificantly affect the general evaluation of tubers. The best clone type is Local Creme Saree.There is no significant interaction between the treatment of SP 36 fertilizer dosage and theclone type on sweet potato crops.
RESPONCE OF SOYBEAN PLANT (Glycine max (L.) Mer) ON EFFICIENCY USE OF NPK FERTILIZER WITH ADDITION OF ORGANIC FERTILIZER Moch Arifin; Juli Santoso; Indriani Prasetyaningsih Ardie
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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Soybean is one of multipurpose plant because soybean can be used as food, as feedingredients, as well as for raw materials of various manufacturing and processed industries.In Indonesia the average requirement of soybean every year reaches 2-3 million tons, whilethe national production of Indonesia only reached 870,068 tons (37.85% of domestic needs).According to the Central Bureau of Statistics (BPS) (2015) recorded imports of soybeansreached 1,525,748 tons. To achieve the target soybean production can be done with nonorganic fertilization. But this agricultural technology is not environmentally friendly becauseof the deposition of heavy metal elements that can pollute the environment. To avoiddeposition of heavy metal elements can be done addition of organic fertilizer. The purpose ofthis research is to know the responce of soybean plant to the efficiency of NPK fertilizer usewith the addition of organic fertilizer. The research method is a simple experiment using randomized block design with theaddition of organic fertilizer that is P0: NPK 100% (Control), P1: NPK 75% + 30 ton/haorganic fertilizer, P2: NPK 50% + 30 ton/ha organic fertilizer, P3: NPK 25% + 30 ton/haorganic fertilizer, P4: organic fertilizer 30 ton/ha and repeated 3 times. The result of observation of plant pod number, number of planting seeds, number ofroot nodule, dry weight of soybean tends, with high clay C-organic content to be moreresponce to the use of NPK 25% fertilizer showed higher tendency with production perhectare (= 2.17 ton/ha) higher than other treatment. It can be said also that with the use ofNPK fertilizer 25% more efficiency.
TESTING OF BLACK SOYBEAN SEED RESPONSE TO SALINITY STRESS IN GERMINATION STAGE Halimursyadah Halimursyadah; hasanuddin hasanuddin; munanzar noorman
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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The objective of this research is to know the response of black soybean seed to salinity stress in germination stage and also to identify the interaction between the two factors. This research has been conducted from April to August 2016 at the Laboratory of Seed Science and Technology of the Faculty of Agriculture, Syiah Kuala University. The experiment was designed using a Factorial Random Block Design. The first factor was the four national varieties of black soybeans (Detam 1, Detam 2, Mutiara 2 and Mutiara 3) and the second factor was the concentration of NaCl (0; 2; 4; 6; 8; and 10 g L-1). There are 24 treatment combinations each repeated three times. The parameters observed were viability (maximum growth potential, germination and time to reach 50% of total germination) and vigor of growing strength (vigor index, relative growth rate, synchrony growth, primary root length of normal seedling germination and dry weight of normal seedling germination. The study showed that Detam 2 were more tolerant than other varieties based on all viability and vigor parameters of growing strength observed. The increase in NaCl concentration at level 4 g L-1 had a very significant effect on the observed parameters indicated by the decrease of viability value and vigor of growing strength. There was a very significant interaction between varieties and concentration of NaCl to viability and vigor of soybean seedling growth that is at the maximum growth potential, primary root length of normal seedling germination and dry weight of normal seedling germination.
Review: Phylogenetic Similarity based on amino acid sequence and Molecular characterization of 3-Phytase from Klebsiella pneumonia ASR1 Sajidan .; Hailu Weldekiros Hailu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Phytase is an enzyme that catalyzes the release of phosphomonester group in phytate, thereby producing lower forms of myo-inositol phosphates and inorganic phosphate.  Phytase has an important role in animal nutrition which improves the bioavailability of nutrients.  Bacteria are one of the potential sources of phytase. For this reason, more screening of phytase producing bacteria strains from environment is needed. The other sources of phytase are fungi, plants, and some animal tissues. The PhyK protein is a phytase enzyme from Klebsiella pneumonia ASR1 and expressed in Escherichia coli, which was sequenced and characterized as a 42 kDa [1], and Crystallized [2]. The objectives are to perform phylogenetic similarity and molecular evolutionary analysis of phytase and to determine active site protein motif of phytase from different sources. The sequence from PhyK was analyzed with Basic Local Alignment Search Tools (BLAST) at http://www.ncbi.nlm.nih.gov  and ClustalW Multiple Sequence analysis program (EMBL-EBI) at http://www.ebi.ac.uk/Tools/msa/clustalw2/ to get similarity of amino acid sequence and phylogenetic trees for protein.  The result shows that PhyK protein from Klebsiella pneumonia ASR1 is 91% similar with PhyK from Raoultella terrigena (emb|AJ575300.1|), 94% similar with histidine acid phosphatase family protein from Klebsiella pneumoniae 342, and 69% similar with glucose-1-phosphatase precursor Agp from Pantoea ananatis AJ13355 (BAK10365.1).The deduced amino acid sequence of the phyK gene from Klebsiella pneumonia ASR1, although containing the functional residues of histidine acid phosphatases, displayed only 31% overall homology with appA from Escherichia coli O104:H4 str. C227-11 (EGT68141.1), 30% similar with glucose-1-phosphatase from Pantoea agglomerans (gb| ABD85282.1|) and 26% to phytase from Aspergillus fumigatus (AAU93517.1). This suggests that PhyK represents a novel subfamily histidine acid phytate-degrading enzyme and is clearly distinct from other previously characterized members of this family. Keywords: Bioinformatics, BLAST, 3-Phytase, PhyK protein, Phylogenetic Similarity
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis Masfufatun .; Noer Kumala; Afaf Baktir
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Dye-Sensitized Solar Cells (Dssc) Using Natural Dyes Extracted From Red Cabbage And Counter Electrode Based Tio2-Graphite Composites Tanti Haryati; Tri Mulyono; Ika Oktavianawati; Wawan Badrianto
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Dye sensitized solar cells (DSSC) has been a popular research topic which benefits in high energy conversion efficiency and less cost in fabrication process. The components of DSSC consist of photoanode, electrolyte, and counter electrode that are arranged as sandwich. The photoanode contains ITO (indium tin oxide) glass covered by TiO2 particles, while the counter electrode contains ITO glass covered by composite of TiO2-graphite as counter electrode and red cabbage extract, as a dye agent. The cell was fabricated in various amount of graphite, i.e 10%; 30%; and 50 %, and time of soaking i.e 2; 13; 24; and 48 hours. The performance of the solar cell per active area of ​​1 cm2 was tested on irradiation of 75 Watt halogen lamp (distance 15 cm) and measured by multimeter and rheostat. The optimum synthesis condition of DSSC was obtained with composition of TiO2:graphite on the counter electrode is 70%:30 %, and soaking time in dye agent is for 24 hours. This cell results in an optimum performance, Isc = 0.028 mA ;Voc = 0.344 mV, FF = 0.29 and = 0.0055 % efficiency. Keywords: DSSC, Red Cabbage, Counter Electrode
Susceptibility of Two Varieties Rice (Oryza sativa L.) on Saline Environment Yuda C Hariadi; Arry Y Nurhayati; A. Endraningrum
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Serealia such as rice, wheat, and maize are very important world food demands, and rice was highest on the nutrional protein status with respect to amino acid composition. Rice (Oryza sativa) has been an important primary cereal for generations that supply around 90% of carbohydrate needs in Indonesia, that every effort to be made in acquiring the fullfillment demand. The research was aimed to investigate the tolerance of two rice varieties Towuti and Ciherang grown on fertile and arid soil on different salinity. Healthy and homogenous three weeks old seedlings were grown in a glasshouse on 0, 50mM, 100mM, 200mM, and 300mM of NaCl on plastic pots with two different soil, under a randomized design and five replicated five times. The growth in terms of leaf area and surface electrical potential difference were observed on a weekly basis for four weeks after the plants had been exposed. The result showed that leaf area and the electrical PD of both rice varieties were significantly reduced under fertile and arid soil under 200mM of NaCl and 300mmM of salinity. It is implied that the photosynthetic process was significantly affected by salinity and was concluded that both varieties were sensitive to salinity at 200mM of NaCl. There was possibility that the protein content was involved on the osmotic change on the salinity process, element supplements were needed, but there is a need for less expensive methods. Keywords: salinity, rice; protein, electrical PD, growth
BIOREDUCTION ADSORBENT (BIOSORBENT): RECOVERY TECHNOLOGY OF HEAVY METAL POLLUTION (CADMIUM/CD) IN POLLUTED LAPINDO WATER SOURCES USING BACTERIA AND DURIAN LEATHER Sueb Sueb; Eka Imbia Agus Diartika; Khasanah Sripalupi; Achib Irmawati
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Sources of water in three villages in Sidoarjo can no longer be consumed because it has been contaminated with heavy metals, one of them is Cadmium (Cd). Cadmium is a heavy metal that is dangerous after Mercury (Hg). Cadmium is dangerous because this element has high risk for blood vessel. Cadmium effects on humans in the long term and can accumulate in the body, especially the liver and kidneys. Handling heavy metal pollution can use biological organism, such as microbes and agricultural wastes. Previous research has identified indigenous bacteria that can reduce heavy metals Cd. From research Wildana et al. (2015) note that the efficiency of Cadmium biosorption by Lactobacillus acidophillus that has been immobilized can be seen from the percentage of removal of metal ions (% R) and the value biosorption capacity (qe). At the inlet concentrations low of 0.5557 ppm, the percentage of removal of metal ions (% R) obtained is the highest at 49.763%. Other researches have also demonstrated the ability of activated charcoal durian leather which can reduce Cd. Therefore, the authors took the initiative to combinate both the adsorbent to the hopes will be more effective at absorbing heavy metals Cd. Bacteria must be isolated prior to the media Na. Durian leather used to be carbonized and activated in advance with KOH.
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
HEMATOLOGICAL CHARACTERISTIC OF THE FEMALE ASIAN VINE SNAKE (AHAETULLA PRASINA BOIE, 1827) I Gusti A. Ayu Ratna Puspita Sari; Endah Sri Palupi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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There is less information about the hematological characteristic of snake in Indonesia. Morphology and morphotic elements size of erythrocyte and leukocyte of the female Asian Vine Snake (Ahaetulla prasina) in North Purwokerto, Indonesia, were studied. Blood was sampled by cardiac puncture and blood cells morphology and size were observed and measured from prepared blood smears. Erythrocyte was nucleated and oval in shape. Leukocyte were lymphocytes, heterophils, eosinophils, basophils and monocytes. The erythrocyte was slightly differ from those of other squamates. Morphology and morphotic size of blood cells provided information about the physiological character of Asian Snake Vine. More comprehensive study is necessary to understand the correlation of blood cells characteristic of snakes with other characteristic.

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