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INDONESIA
Jurnal Ilmu dan Kesehatan Hewan
Published by Universitas Udayana
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions Veterinary
Jurnal Ilmu dan Kesehatan Hewan menerima naskah ilmiah original (belum pernah dipublikasikan) dalam bidang veteriner atau yang terkait dengannya. Jurnal Ilmu dan Kesehatan Hewan dikelola oleh Program Studi Magister Kedokteran Hewan Universitas Udayana dan terbit dua kali setahun.
Arjuna Subject : -
Articles 45 Documents
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Polimosfisme Lokus Mikrosatelit BM1329 dan Hubungannya dengan Calving Interval pada Sapi Bali Made Raysa Merliana; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle plays an important role in supplying beef in Indonesia because it has good quality, high fertilization and low fat percentage. The aim of this research was to characterize the Bali cattle genetic using molecular marker of microsatellite BM1329 and its relation with calving interval. The data was taken from 19 cattles that belong to UD Sari Laba, Bangli, Bali. The association between BM1329 marker and calving interval was analyzed using General Linear Models concept. The result showed that the duration of calving interval for the Bali cattle was 314 – 451 days. There were 4 alleles found in the locus of microsatellite BM1329. The alleles size varied from 144 to 150 bp, in which, the allele 146 had the highest frequency (71.05%). The observed heterozigosity (Ho) and the expected heterozigosity (He) and the PIC were 0.474, 0.478, and 0.435 respectively. The statistical analysis showed that microsatellite BM1329 has no assocition with the calving interval (P>0.05).
Evaluasi Uji ELISA dengan Serum Lapangan sebagai Crude Antigen di Bali Pratiwi Devi GM; I.M. Damriyasa; N.S. Dharmawan
Veterinary Science and Medicine Journal Vol 2 No 1 (2014)
Publisher : Udayana University

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Abstract

The purpose of this study was to evaluate the ELISA test using field serum and to determine the incidence rate of cysticercosis of T. saginata in Bali. Serum’s sample obtained from Bali cattle that slaughtered at the abattoir and Bali cattle owned by the farmers. The result of sera examination showed that 237 (87.7%) were positive of antibodies of cysticercus of T. saginata. Furthermore, as many as 90 (33.33%) of the 270 Bali cattles, their feces were also taken for examination of worm eggs. The results of stool examination showed that 80 (88.9%) were infected with trematodes and 14 (15.5%) were infected with a mixture of trematodes and nematodes. By comparing the results of ELISA and stool examination using the sensitivity and specificity approach or Table 2 x 2, the results showed that there was a cross-reaction between Cysticercus of T. saginata and trematode. Further effort to purify the antigen of cysticercus of T. saginata is still needed to improve its sensitivity and specificity.
The Effect of Soyabean Extender on Viability and DNA Integrity of Kintamani Dog Sperm on Cold Storage I Made Yoga Windu Pradana; Wayan Bebas; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

The objective of this study was to evaluate the viability and integrity of canine semen diluted in egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. Semen was collected by manual manipulation from an apparently healthy kintamani dog stud. The semen was subjected to gross and microscopic examination to determine its viability following dilution in different extenders. Only semen with motility rate of 60% or higher was used in this study. Semen samples were diluted in Tris-citrate-glucose-soya bean and egg yolk glucose-citrate extenders at two levels of dilution; sperm to extenders ratio of 1:2 and 1:3. Following semen dilution, evaluations were made on sperm motility, percentage of live sperm, and DNA integrity at 0 hour, 3 hours, and 6 hours under 5oC of storage. Data was analyzed by multivariate analysis of variance (MANOVA).The present results showed a high significant different (p<0.01) in sperm motility, percentage of live and DNA integrity between treatment with egg yolk glucose-citrate and Tris-citrate-glucose-soya bean.  The result also showed that percentage of live sperm and DNA integrity of semen at 1:2 dilution difference significantly (p<0.01) to that of 1:3 dilution. The sperm viability and integrity were significantly difference (p<0,01) between the duration of storage at 5°C. Thus, the current study indicated that Tris-citrate-glucose- soya bean extenders  was sufficient to maintain motility, viability, and DNA integrity of kintamani dog  spermatozoa during storage at 5 oC. Further research should be conducted to evaluate the fertility of spermatozoa following dilution with these extenders.
Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus) I Ketut Suardita; I Ketut Puja; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals
Profil Sel Darah Merah Monyet Ekor Panjang (Macaca fascicularis) Obes yang Hidup Liar di Pura Uluwatu, Bali Agus Wawan Darmawan; I Gede Soma; I Nengah Wandia
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

The aim of this study was to determine the profile of red blood cells in obese long-tailed macaques. A totalof 16 long-tailed macaques was arrested, and then blood samples were collected aseptically and stored inEDTA vacutainer. Twelve out of 16 were classified obese which is determined by body mass index (BMI).The results showed that the average number of erythrocytes 6.08±0.87 (x1061,2*/mm), the levels ofhemoglobin 11.98±1.59 (g/dl), hematocrit value 37.5±3.55 (%), MCV 62.12±5.35 (fl), MCH 19.91±2.06(pg), and MCHC 32.15±4.22 (g/dl) in average. It can be concluded that the red blood cell profile of theobese long-tailed macaques is still in normal range.
Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang pada Sapi Bali Yesy Febnica Dewi; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle has an important role to fulfill the meat demands in Indonesia. The improvement of molecular technology will facilitated bali cattle genomic selection. The aim of this research was to verify association between BMS1282 microsatellite locus and repeat breeding in bali cattle. Totalling 52 bali cattle blood samples was taken from UD Sari Laba, Bangli, Bali as DNA resources. DNA was extracted using blood mini Kit qiagen and microsatellite locus was amplified by Polymerase Chain Reaction (PCR). Alleles were separated with 6% PAGE and visualized using silver stain. There were 5 alleles in BMS1282 locus which the alleles size varied from 148 to 156 bp. Allele 152 was the higest frequency (54.81%) and allele 156 was the lowest frequency (1.92%). The value of heterozygosity (Ho), He, and PIC was 0.346, 0.626, and 0.569. consecutively. Statistical analysis revealed that BMS1282 locus has no assosiation with the repeat breeding (P>0.05). This result indicates that BMS 1282 locus has no important role in regulating the repeat breeding.
Seroprevalensi Toxoplasma gondii pada Kambing dan Bioassay Patogenitasnya pada Kucing Ni Made Yunik Novita Dewi Dewi; I Made Damriyasa Damriyasa; Nyoman Adi Suratma
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The study aimed to determine seroprevalence of Toxoplasmosis in goats sloughtered at Kampung Jawa, Denpasar, Bali and to evaluate their pathogenicities through bioassay in cats.One hundred serums and meats of goats were collected. Anti-Toxoplasma gondii antibody was determined using Indirect Haemaglutination (IHA) test. The pathogenicity bioassay of Toxoplasma gondii was carried out through inoculating the meats of goats which had seropositive of Toxoplasma gondii to the cats. The pathogenicity was evaluated using the intensity of oocyte sheding from the cats. The result showed that the seroprevalence of Toxoplasmosis was 46%. There was not significant difference between pathogenicity of Toxoplasma gondii in cat inoculated with meat of goat which had a high and low titer of antibody against Toxoplasma gondii.
Seroprevalensi Infeksi Virus Newcastle Disease dan Deteksi Paramyxovirus Pada Itik di Peternakan dan Pasar Unggas di Bali I GBA Purwanda; I Gusti Ngurah Kade Mahardika; Gusti Ayu Yuniati Kencana
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

Seroprevalence of Newcastle Disease Virus (NDV) infection and the presence of paramyxovirus on ducksat the farmland and the poultry market in Bali have not been known. The purpose of this study was todetermine the comparison of seroprevalence of NDV infection and the presence of paramyxovirus on duckat farmlands and poultry markets in Bali. Locations sampled were Gelgel, Tojan, Akah, Takmung, Tusanvillages in Klungkung Regency, as well as Mengwi, Mengwitani, Lukluk, Sangeh, and Blahkiuh villages ofBadung Regency. The poultry market samples were Galiran of Klungkung Regency, and Bringkit ofBadung Regency. Serum samples and cloacal-tracheal swabs were taken using stratified-random samplingfrom adult ducks of both markets and farmland that had  more than 500 individuals in a flock. Samplingwas carried out every month for 6 months. Antibody against NDV was detected with InhibitionHaemagglutination test (HI). Tracheal and cloacal swabs were propagated in fertile chicken eggs of 9-11days old. Paramyxovirus was detected by the haemagglutination (HA) test and Reverse TranscriptasePolymeraseChain Reaction (RT-PCR). The correlation between NDV seroprevalences at farmland andpoultry markets was analyzed using non-parametric test of Chi-square. The results showed that theseroprevalence of NDV on March until August 2012  reached 45% on farmlands in both regencies, while inthe markets were up to 32.6%. There was no correlation between NDV seroprevalence at farmlands andpoultry markets in the two regencies (r = 0.522, P> 0.05). The paramyxoviruses detected were APMV-5and APMV-8, while NDV was not found.
Deteksi Antibodi Mycobacterium tuberculosa bovis pada Sapi di Wilayah Kabupaten Buleleng, Bangli, dan Karangasem Provinsi Bali Putu Gede Widiarsa Putra; Nengah Kerta Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

Bovine tuberculosis (BTB) is one the most widespread zoonotic diseases and has recently reemerged as a major health concern. The disease is caused by the Mycobacterium tuberculosis complex (MTBC) that primarily affects humans as its main host, and that is able to attack more hosts such as domestic animals, pet animals, and wild animals. This Cross-Sectional Study was carried out to determine the seroprevalence of BTB in Districts of Buleleng, Bangli, and Karangasem Province of Bali. One hundred and eighty cow blood samples were collected, and the serological status of BTB was detected using Elisa method. The results showed that the seroprevalence of BTB in District of Bangli was 2.22%, while, those was negative in Districts of Buleleng and Karangasem. The positive seroprevalence of BTB in Bangli indicates that the same research must be conducted throughout the districts in the Province of Bali.
Prevalence of Ear and Eye Disorders in Kintamani Dog Ni Made Devityasih Perayadhista; I Ketut Suatha; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

This observational study was undertaken to evaluate the prevalence of ear and eye disorders in Kintamanidog. All data were gathered from registers of veterinary clinic located at Denpasar, Badung, and Gianyar.Various aspects concerning ear and eye disorders were collected by questionnaires. The total of Kintamanidog recorded from 2009 until 2013was 477 dogs. The result of this study showed that the prevalence ofexophthalmos (protrusion of the eye ball) and glaucoma were rare (0%), the cataract was occurred in onedog (0.2%), otitis was occurred in two dogs (0.4%), and conjunctivitis was occurred in three dogs (0.6%).All Kintamani dogs with ear and eye disorders were the male and thier ages were more than 12 months. Inconclusion, this study indicates  that the Kintamani dog has a lower problem of ear and eye disorders. In thefuture, this low prevalence of the problem hopefully can be maintained or even decreased by giving goodbreeding management.

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