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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 20 Documents
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Search results for , issue "Vol 16 No 3 (2015)" : 20 Documents clear
Seroprevalensi Penyakit Tetelo pada Peternakan Itik dan Pasar Galiran di Kabupaten Klungkung, Bali (NEWCASTLE DISEASE SEROPREVALENCE IN LIVESTOCK DUCK AND MARKETS GALIRAN OF KLUNGKUNG RECIDENCE, BALI) I Komang Wahyu Yuliana; Gusti Ayu Yuniati Kencana; I Nyoman Suartha
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ducks are one of the birds that play a role in the spread and transmission of Newcastle disease virus.Newcastle disease infection in ducks are rarely accompanied by clinical symptoms, so the ducks couldpotentially spread the disease to other birds. In general, the distribution of ducks is going from farm toMarket Poultry and vice versa. The aim of this study was to determine the seroprevalence Newcastledisease infection in ducks reared on farms and sold at market Galiran Klungkung regency. The sampleused in this study was serum unvaccinated ducks ND. The samples used were 420 samples taken from thefarm and from Klungkung Galiran Market from March to August 2012.Serum samples were tested for thepresence of NDV antibody by using hemagglutinationtest(Haemaglutination Inhibition Test / HI test) atthe Biomedical Laboratory of the Faculty of Veterinary Medicine Udayana University. The results showedthat the seroprevalence Newcastle disease virus in ducks in Galiran Market were 33.3% and in farmsamounted to 46.2%. There are differences in seroprevalence Newcastle disease (p <0.05) in March, June,and August. Overall seroprevalence in Galiran Market and Newcastle disease in livestock in Klungkungregency equal to 39.8%. It can be concluded that Newcastle disease seroprevalence in Klungkung regency isquite high and might be potentially transmit the virus to other poultry. Therefore, periodic monitoring isnecessary as an effort to early prevention.
Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
Pemberian Pregnant Mare Serum Gonadotropin Sebelum Perkawinan dan Jamu Selama Kebuntingan untuk Memperbaiki Performa Anak Domba (ADMINISTRATION OF PREGNANT MARE SERUM GONADOTROPIN AND JAMU DURING GESTATION TO IMPROVE FOR LAMB PERFOMANCE) Andriyanto .; Ridi Arif; Adi Winarto; Leo Sapelani Soinbala; Bondan Achmadi; Aulia Andi Mustika; Diah Nugrahani Pristihadi; Amrozi .; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Administration of pregnant mare serum gonadotropin (PMSG) hormone prior to mating increasesquality of lamb born on one and two litter sizes, but in three litter size the motality is higher. Administrationof traditional herbal medicine “jamu” consisting of ginger,green chiretta, cinnamon, Zingiberzerumbet, andpepper during gestation increases performance of lambs. This research was conducted to explore theeffectivity of PMSG injection prior to mating and jamu administration during gestation to increase lambsperformance. Eighteen priangan fat-tail ewes with weight around 20-25 kg were injected with PGF2á at adose of 10 mg/ewe twice with 11 days interval to synchronize estrous cycle. Injection of PMSG at a dose of200 IU/ewe was conducted at the second PGF2á injection. The experimental ewes were mated naturally anddivided into a randomized design with a 2 x 3 factorial arrangement with three replications. The firstfactor was dose of PMSG with two levels i.e., 0 and 200 IU/ewe. The second factor was dose of jamu withthree levels i.e., 0, 15, and 30 mL/ewe. Jamu was administered orally every week during gestation. Injectionof PMSG and administration of jamu decreased prenatal mortality, increased ratio of lamb per ewe, increased total lamb born with average birth weight and total birth weight were higher in jamu at a doseof 15 and 30 mL/ewe by 30.02 and 31.76%, repectively. During the first month postnatal, lambs born toewes injected by PMSG and administered jamu had higher number of lambs survive, average weight, totalweight around 3 times as compared to control. It was concluded that injection of PMSG prior to mating andadministration of jamu during pregnancy increase lamb birth weight and improve the quality of lamb.
Perlindungan Protein Menggunakan Tanin dan Saponin Terhadap Daya Fermentasi Rumen dan Sintesis Protein Mikrob (PROTECTION OFPROTEINUSINGTANNINS AND SAPONINS OF RUMEN DIGESTIBILITYAND MICROBESSYNTHESISPROTEIN) Ana Shofi Ani; Retno Iswarin Pujaningsih; Widiyanto .
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this experiment was to examine protection of protein using tannins and saponins toimprove rumen digestibility and microb-mediated protein synthesis in vitro. Rumen fluids used as inoculumwas collected from a composite of two female adult fistulatedongole cattle weighed of ±300 kg with theage of five years old. The experimental design used in this study was a completely randomized design withsix treatments and three replication of each treatment. The six treatments consisted of T0: Proteinconcentrates without protection, T1: protein concentrates protected with 1.2% saponin, T2: proteinconcentrates protected with 0.5% tannin and 0.9% saponin , T3: protein concentrates protected with 1.0%tannin and 0.6% saponin, T4: protein concentrates protected with 1.5 % tannin and 0.3% saponin and T5:protein concentrates protected with 2.0% tannins. The result showed that treatment with tannin, saponinand their combination had a significantly affect (P<0,05) on the level of ammonia (NH3), the total volatilefatty acids (VFA), and total protein. Protection of proteins with combination of 1,0% tannin and 0.6%saponin resulted in best effect on feed protein as shown by its NH3 concentration, total VFA and totalprotein. This indicates the level of protection of feed protein can improve rumen digestibility and microbesmediatedprotein synthesis, as showed in the concentration of N-NH3, total VFA and total protein.
Behavior of Bali Starling at Bali Barat National Park and Nusa Penida Island (PERILAKU JALAK BALI DI TAMAN NASIONAL BALI BARAT DAN PULAU NUSA PENIDA) Sudaryanto .; Tjut Sugandawaty Djohan; Satyawan Pudyatmoko; Jusup Subagja
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Jalak Bali (Leucopsar rothschildi) sejak tahun 1966 dimasukan oleh International Union forConservation of Nature (IUCN) Red List of Threatened Species dan Convention on International Trade inEndangered Species of Wild Fauna and Flora (CITES). Jalak Bali dikelompokan sebagai satwa terancampunah dengan kategori kritis (Critically Endangered) dan di Indonesia telah dilindungi sejak tahun 1970.Tujuan penelitian ini adalah untuk mengetahui perilaku Jalak Bali di Taman Nasional Bali Barat danPulau Nusa Penida untuk konservasi burung tersebut. Metode yang digunakan adalah scan samplingdengan instataneous sampling. Perilaku Jalak Bali di Taman Nasional Bali Barat terdiri dari terbang17%, makan buah 3%, makan serangga 1%, menelisik bulu 15%, menegakkan jambul 6%, bobbing 7%,berkicau 40%, minum 0,5%, dan berjalan 10%. Sementara di Nusa Penida pada musim hujan terdiriterbang 13%, makan buah 19%, makan serangga 4%, menelisik bulu 7%, menegakkan jambul 7%, bobbing8%, berkicau 11%, minum 1%, 1% mandi, berjalan 16% , bersarang 2%, mengeram 9% dan mengasuh anak3%, dan di Nusa Penida pada musim kemarau terdiri terbang 11%, makan buah 9%, makan serangga21%, menelisik bulu 7%, menegakkan jambul 6%, bobbing 7%, berkicau 9%, minum 2%, mandi 1%, berjalan18% , dan bersarang 8%. Jalak Bali berkembang biak di Nusa Penida pada musim hujan dan musimkemarau, tetapi di Taman Nasional Bali Barat pada musim hujan.
Keragaman Spesies dan Genetik Bakteri Staphylococcus pada Ikan Tuna dengan Analisis Sekuen 16s rRNA (SPECIES DIVERSITY AND GENETIC OF STAPHYLOCOCCUS BACTERIA IN TUNA FISH BY USING 16S rRNA SEQUENCE ANALYSIS) Putu Mei Purnama Dewi; I Nengah Kerta Besung; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tuna industry belongs to high economic value fish of marine fisheries commodity. Bacteria found intuna might have impact on consumer health. The purpose of this research was to determine the geneticvariation of Staphylococcus in Kedonganan fish market, Kuta, Badung, Bali by sequence analysis of 16SrRNA. Staphylococcus bacteria was isolated from feces samples of 30 tuna. After identification with Gramstaining, Staphylococcus colonies were transferred to the medium chelex 10% for DNA extraction, 16SrRNA gene amplification by polymerase chain reaction (PCR), and electrophoresis. PCR results weresequenced, and the sequence obtained was edited using MEGA 5 and then BLAST was applied to confirmthe species. Genetic variation was determined by analysis of polymorphic sites with MEGA 5. The identifiedbacteria were Staphylococcus sciuri and Staphylococcus haemolyticus. Genetic distance of two isolates S.sciuri are close. The Other species that were identified were Enterococcus faecalis and Macrococcus caseolyticus.Conclusion on this research is Staphylococcus bacteria and two species S.sciuri have many variation.
Preservasi Semen Kambing Peranakan Etawa dalam Pengencer Tris dan Sitrat Kuning Telur dengan Penambahan Sodium Dodecyl Sulphate (THE PRESERVATION OF ETTAWA GRADE BUCK SEMEN IN TRIS AND CITRATE EGG YOLK DILUENTS SUPPLEMENTED WITH SODIUM DODECYL SULPHATE) Nur Hidayati; Raden Iis Arifiantini; Dondin Sajuthi
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was conducted to determineSDS concentration also to compare Tris egg yolk and citrateegg yolk on the quality of ettawa grade chilled semen. The study consist of two experiments. The firstexperiment was to determine the best SDS concentration in Tris egg yolk diluents and the second experimentwas to compare the SDS suplementation in tris and citrate egg yolk in the quality of ettawa grade chilledsemen. The semen were collected from three bucks, immediately after collection the semen evalutedmacro and microscopycally. Semen demonstrate more than 70% motility and less than 10% spermatozoaabnormality, individually divided into four tube, each tube was diluted in egg yolk supplemented with 0;0,025; 0,05; and 0.075% SDS then stored at 5°C for 72 hours. The quality of chilled semen was observefor motility and viability every 12 hours. Result demonstrated that 0.05% SDS was the best concentrationcompared to others. In the second experiment,the semen were diluted in four different diluents, it wereTris egg yolk (TEY), Tris egg yolk with 0.05% SDS (TEYS), citrate egg yolk (CEY) and citrate egg yolk with0.05% SDS (CEYS). Result demonstrated that the mean value of motility and viability of spermatozoa inTEYSdiluents (67.08 ± 6.43% and77.07 ± 6.78%) was higher (p<0.05) than CEYS (60.42 ± 9.05% and72.31± 7.45%), TEY (59.23 ± 9.41% and71.21 ± 8.56%) and CEY (53.45 ± 11.33% and67.74 ± 8.90%). In conclusion,supplementation of 0.05% SDS in TEY was maintained best sperm quality compared to other diluentsduring preservation.
Konsentrasi Asam Lemak Terbang dan Glukosa Darah Domba Ekor Tipis yang Diberi Bungkil Kedelai Terproteksi Tanin (VOLATILE FATTY ACID CONCENTRATION AND BLOOD GLUCOSE ON THIN-TAILED SHEEP GIVEN WITH TANINE-PROTECTED SOYBEAN MEAL) Siti Nuraliah; Agung Purnomoadi; Limbang Kustiawan Nuswantara
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims to analyze the influence of tannin-protected protein source feed to livestock productivitybased on its influence on methane production, Volatile fatty acids (VFA) production, and blood glucose inthe use of tannin-protected protein on complete feed in thin-tailed ram. The study uses thin-tailed ramaged about 8 months, as many as 16 rams with body weight of 11.81±1.65 kg. The researchusesa completerandomized design(CRD) withfour treatmentsandfourreplications. The treatments areP0:15% soybeanmeals without tannin protection in complete feed, P1:15% soybean meals with 0.5% tannin protection incomplete feed, P2:15% soybean meals with 1% tannin protection in complete feed and P3:15% soybeanmeals with 1.5% tannin protection in complete feed. The results indicates that administration of tanninsin soybean meal in complete feed showed significant effect (P <0.05) on blood glucose, the production ofpropionic acid in the 3rd hour, but the VFA production at hour 0, hour 6, as well as methane productionshowed no significant effect (P> 0.05). The conclusion is that 15% protected soybean meal with 1% oftannin in the complete feed is able to contribute to the proportion of propionate and contribute to increasedblood glucose on a thin-tailed ram but can not to reduce methane production.
Identifikasi Secara Serologi Galur Virus Flu Burung Subtipe H5N1 Clade 2.1.3 dan Clade 2.3.2 pada Ayam Petelur (SEROLOGICAL IDENTIFICATION OF AVIAN INFLUENZA STRAIN VIRUS SUBTYPE H5N1 CLADE 2.1.3 AND CLADE 2.3.2 FROM LAYER) Aprilia Kusumastuti; Syamsidar .; Agustin Zaharia Paderi; Arini Nurhandayani; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to know avian influenza (AI) infection in field by using serology test in threemarketing area of AI vaccines. Haemagglutination inhibition methode was used in this test. There werefour antigen strains of AI subtype H5N1 clade 2.1.3 (AIstrainA/Chicken/West Java/PWT-WIJ/2006, AIstrain A/Chicken/Garut/BBVW-223/2007, AI strain A/Chicken/West Java-Nagrak/30/2007, and AI strainA/Chicken/Pekalongan/BBVW-208/2007) and 2 antigen strains of AI subtype H5N1 clade 2.3.2 (AI strainA/duck/Sukoharjo/BBVW-1428-9/2012 and AI strain A/duck/Sleman/BBVW-1463-10/2012) was used inthis study for HI test. The result presents that 93,33% chicken farms in three marketing area of PT. SanbioLaboratories have positive antibody titre to AI subtype H5N1 clade 2.1.3. This titre may be obtained fromAI clade 2.1.3 vaccination. From 15 samples, 92,86% are positive to AI subtype H5N1 clade 2.3.2A/duck/Sukoharjo/BBVW-1428-9/2012 and 92,31% are positive to A/duck/Sleman/BBVW-1463-10/2012 evenwithout AI clade 2.3.2 vaccination. This antibody titre may be obtained from AI clade 2.1.3 vaccine crossprotection or field infection.
Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

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