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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 19 Documents
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Search results for , issue "Vol 19 No 3 (2018)" : 19 Documents clear
Anemia and Eosinophilia in Traditional Goat Farmers: Early Markers of Strongyle Zoonoses (ANEMIA DAN EOSINOFILIA PADA PETERNAK KAMBING TRADISIONAL: PENANDA AWAL ZOONOSIS STRONGYLUS) Insy Nafisah Taufik; Henhen Heryaman; Rini Widyastuti; Mas Rizky A. A. Syamsunarno; Mohammad Ghozali
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The spread of zoonoses between humans and animals can increase with the presence of specific contacts between the two. As well as intensive contact between farmer and goat. Anemia is a common hematologic change encountered in infection and zoonoses so it can be used as an early indicator for zoonoses. Nevertheless, anemia itself cannot lead to a specific etiology thus differential leukocyte count and goat fecal examination can be a good supporting test. Understanding the etiologies of the disease is critically important in preventing the decline in human’s quality of life as well as economic losses. This cross-sectional study included 30 farmers who were recruited by purposive sampling method. They agreed to do a CBC examination with 22 parameters. From the test, 37% of them showed mild anemia. There were 45% farmers with microcytic hypochromic anemia while the rest had normocytic normochromic anemia. There was an increased number in eosinophil and segmented neutrophil from anemic farmers, 18% and 9% respectively. Besides, 13% of goats physical examination indicated as below normal conditions characterized by lean bodies, lymph node swelling, and pale mucosa. Whereas 38% goat’s fecal test showed evidence of Strongyle eggs. The research suggests a possible interrelation between animals, humans, and the environment with an increased risk of zoonoses. Anemia and eosinophilia that occur can be an early marker which will be decisive not only for the success of the disease progression reduction but also the long term suppression of the economic loss.
Potensi Toksin Binders untuk Mengurangi Efek Mikotoksin Zearalenon terhadap Panjang, Bobot, dan Jumlah Fetus Mencit (TOXIN BINDERS HAS THE POTENTIAL TO DECREASE ZEARALENONE MYCOTOXIN ON FETAL LENGTH, WEIGHT AND LITTER SIZE OF PREGNANT MICE) Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Ragil Angga Prastiya
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Corn is a source of feed with protein content to increase livestock production. Zearalenone is a mycotoxin produced by Fusarium graminearum obligate in corn. The mycotoxins allegedly can cause pregnancy disorders to fetal malformations. Using toxin binders to inhibit zearalenone mycotoxins. The purpose of this study was to determine the potential of toxin binders in reducing the effect of zearalenon on the length, weight and fetal litter size of pregnant mice (Mus musculus). This study used 20 balb/c mice divided into control group (C-) without toxin binders and zearalenone; (C+) was given only zearalenone 0.1 mg / mice / day; and treatment groups T1; T2; T3 was given zearalenone 0.1 mg / mice / day and toxin binders 0.5; 1; 2 mg / mice / day. The toxin binders and zearalenone was done with orally for 14 days. The data were analyzed using Analysis of variance test and continued by Duncan test. The results showed that C-, C+, T1 were significantly different (p<0.05) with T2 and T3 on the fetal length. The fetal weight showed C-, T1, T2, T3 was significantly different (p<0.05) with C+. The result of fetal litter size showed that were not significantly different (p>0.05) between treatment groups. It was concluded that toxin binders could decrease the effect of zearalenon thus increasing the length and weight of the fetus but not affecting the fetal litter size of pregnant mice (M. musculus).
Prevalensi dan Pola Gen Extended Spectrum B-lactamase Bakteri Usus Sapi Perah dan Penduduk Sekitar Peternakan di Surabaya Triffit Imasari; Wiwiek Tyasningsih; Eddy Bagus Wasito; Kuntaman Kuntaman
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Since identified in 1980s, the multiple drug resistant organisms such as Extended Spectrum Beta Lactamases (ESBL) producing bacteria is increasing. These bacteria Enterobacteriaceae strain are mostly resistant against third and also fouth generation cephalosporin. ESBL-producing bacteria are identified in both of human, environment and also in animal. There are three main ESBL genes that are commonly found namely SHV, TEM and CTX-M. The aims of this study were to explore the prevalence and pattern of ESBL gene among dairy cows and people around the farm. The faecal samples were collected from dairy cows and people around the farm, cultured on MacConkey agar supplemented with cefotaxim 1 mg/L, incubated at 37oC for 24 hours. Then the growing colony were tested for ESBL producer by Double Disk Synergy Test (DDST), then followed by Polymerase Chain Reaction (PCR) for ESBL gene. Total sampling technique with inclusion and exclusion criteria, Total 49 samples were collected, consisting of 25 dairy cows faeces and 24 people faeces. Among these, were identified 18 samples (72%) positive in dairy cows and 19 samples (79.1%) positive results in the people around the dairy farm. The ESBL gene, SHV, TEM, CTX-M were identified dairy cows were zero for SHV, TEM (12%), CTX-M (72%) while in people around the farm SHV (25%), TEM (16.7%), CTX-M (66.7%). There were significant different (p < 0.05) between dairy cows and people around the farm, of SHV ESBL gene and not different (p>0.05) of TEM and CTX-M ESBL gene respectively. The ESBL genes have spread among dairy cows and people around the farm.
Protein Rekombinan Outer Membrane Protein-31 dengan Superoxide Dismutase pada Pichia Pastoris Berpotensi Sebagai Kandidat Vaksin Brucellosis Arizah Kusumawati; Sri Kartika Wijaya; Ulfatul Husnaa; Yana Rubiyana; Adi Santoso
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Brucellosis is zoonotic disease caused by Brucella infections transmitted to human either direct contact with animals or consume their products. This becomes problematic in the world especially in endemic countries of Brucella and development countries. Brucella melitensis is one of pathogenic species from genus Brucella. Combining immunogenic side both the outer membrane protein 31 (OMP31) derived from B. melitensis and superoxide dismutase (SOD) derived from B. abortus has the potential to be developed as a candidate for recombinant vaccine. In this study, the expression protein of fusion OMP31-SOD inserted in pPIC9K plasmid with extra His-tag sequence, intends to be developed as a recombinant protein vaccine. Recombinant plasmids pPIC9K+OMP31-SOD were transformed into Pichia pastoris KM71 using electroporation method. Selection of transformants was as performed using geneticin® concentrations of 0.25 mg/mL – 4 mg/L Selected transformants were grown to express the OMP31-SOD recombinant protein. Protein expression was confirmed using both of dot-blot method and electrophoresis with Coomassie Brilliant Blue (CBB) staining. The OMP31-SOD recombinant protein was successfully expressed extracellular by Pichia pastoris KM71 clones 3, 4, and 6 with a molecular weight approximately 40 KDa.
The Bioactive Compounds of Avicenia sp Stem Extract Improved the Viability of Fish Challenged with Aeromonas hydrophila (SENYAWA BIOAKTIF EKSTRAK BATANG AVICENIA SP MENINGKATKAN VIABILITAS IKAN YANG DITANTANG DENGAN AEROMONAS HYDROPHILA) Alimuddin Ali; Iwan Dini; Darminto Darminto; Hartati Hartati; Herlina Rante
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Aeromonas hydrophila is one of bacteria as major diseases for the Motile Aeromonads Septicemia (MAS). The aims of this research to get a substance which potential as bioactive of stems bark of Avicennia sp. for increase fish viability which exposed of A. hydropila. The samples were extracted by using ethanol 70% and then evaluated of antibacterial activities by paper disk diffusion agar technique. The active extracts were partial purified by chromatography column. The fraction of substance was show activities against bacteria analyzed by GC-MS. The results were found four peak and predicted as (1) 2-propenoic acid, 3-[3, 4-dimethoxyphenyl]-methyl ester (2) 3, 4-dimethoxycinnamic acid, (3) 1, 1-dimethyl guanidine (4), Methyl 10-oxostearate. The active fraction was isolated from stem bark of Avicennia sp. should be further studied in animal models for in vitro efficacy and toxicity. The percentage of in vivo towards test fish viabilities showed the difference between treated and untreated. The percentage of viability ranged from 35 to 83.33% while control was 91.67%. Based on finding, we suggest that Avicenia sp. bark extract was increasing of fish viability.
Radiographic Evaluation of Rabbit Femur Implanted Bali Cattle Bone Graft I Wayan Wirata; Steven Dwi Purbantoro; Luh Made Sudimartini; IWN Fajar Gunawan; AA Oka Dharmayudha; IGAG Putra Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bone xenograft from cattle bone is commonly used to treat a comminuted fracture case. This study aims to know the process of fractured-femur bone healing in rabbit post-implantation powder bone graft from cortical femur bone of Bali cattle evaluated by radiographs. Ten male local rabbits were used in this study, which were divided into 2 groups randomly. Group I (KI) as control, the diaphysis of femur bone was drilled with a diameter of 5 mm without implanting the bone graft, while Group II (KII), the diaphysis of femur bone was drilled 2 holes with a diameter of 5 mm each and with distance 20 mm, substituted with mineralized powder bone graft for the proximal hole (KIIa) and demineralized powder bone graft for the distal hole (KIIb). Fracture healing evaluation was done at week 0 (24 hours), 2, 4, and 6 postoperative by monitoring the growth of callus, fracture line, and union process with radiograph based classification according to Hammer et al., tabulated statistically, and presented descriptively. The results showed that KI and KII were in the sequel of fracture healing but had not reached remodeling phase perfectly. In conclusion, mineralized and demineralized powder bone graft used in this study was as osteoconductive and the use of bone graft shows no different significance and time shows different significance to fracture healing.
Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT) Rini Widyastuti; Jaqueline Sudiman; Tyagita Tyagita; Mas Rizky Adipurna Anggun Syamsunarno; Sony Heru Sumarsono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes secondary sperm abnormalitties on mouse sperm.
Deteksi Antibodi dan Isolasi Toxoplasma gondii pada Itik lokal di Bali (DETECTION ANTIBODIES AND ISOLATION OF TOXOPLASMA GONDII IN DOMESTIC DUCK IN BALI ) Made Dwinata; Ida Bagus Made Oka; I Made Damriyasa
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to determine detection antibodies and isolation T. gondii infection in domestic duck in Bali. A total, 188 domestic ducks sera were examined using indirect haemaglutination test kit (IHA). Heart, brain and muscle of seropositive IHA test were used for isolation with pepsin-HCL digestion and bioassay in mice and cat. The result of these research showed that 47 (25%) ducks were found to be positive for T. gondii antibodies at the cut-off e” 1:64. The seroprevalence in male and female duck were 27,8% and 22,4% respectively, however, statistical analysis showed that the difference was not significant (P>0,05). The seroprevalence in cage and free-range duck were 18,7% and 29,2% respectively, but the difference was not statistically significant (P>0,05). The antibodies titer ranging from 1:64 to 1:2048. Also, viable T. gondii was isolated from seropositive duck by bioassay mice and cat. Most of the isolated strains were avirulent to mice. This study showed that domestic duck could have a potensial role in transmitting toxoplasmosis to human in Bali.
Identifikasi Gangguan Reproduksi pada Ovarium Sapi Potong yang Mengalami Anestrus Postpartum Panjang (IDENTIFICATION OF REPRODUCTIVE DISRUPTION ON PROLONGED POSTPARTUM ANESTRUS BEEF COW OVARIES) Bayu Rosadi; Teguh Sumarsono; Fachroerrozi Hoesni
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Postpartum anestrus is the major factor causing elongation of the calving interval and in consequense, lowering beef cow productivity. Anestrus condition is closey related to inactive ovary condition. The research was conducted to find out reproductive disorders in ovarian of beef cattle cow that underwent prolonged postpartum anestrus (more than 3 months). One hundred and fifty heads post partum anestrus cow were selected. The general body condition were evaluated based on Body Condition Score (BCS). The types of reproductive disruption in ovary were determined by rectal palpation method. The cows observed had 2.0 to 3.2 (2.6 ± 0.7) in BCS. The result showed that 37.68% of prolonged postparum anestrus cow had ovarian disorders. The types of ovarian disoreders detected were hypofunction (19.32%), cystic follicle (8.21%), persistent corpus luteum (4.5%), atrophy (1.93%) and partial agenesis (0.97%).

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