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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 262 Documents
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STRUKTUR, KOMPOSISI DAN STATUS TUMBUHAN OBAT DL KAWASAN HUTAN TAMAN NASIONAL ALAS PURWO SYAMSUL HIDAYAT
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Alas Purwo National Park is one of representative tropical low rain forests at eastern Java. There are many natural attractive sites and historical legend, which lead to, famous for ecotourisme activities. There are also rich of flora and fauna that some of them endemic and endangered species. Medicinal plant is one important material on the forest, which still unexplored more. Ten sampling transects were established in some sector of Alas Purwo for inventory of wild medicinal plants. The result shows that at least 5 species have been categorized as an endangered plant. Meanwhile, the structure and composition of endangered medicinal plants is not better than common medicinal plants.
GENETIC DIVERSITY OF SKIPJACK TUNA (Katsuwonus pelamis) FROM JEMBRANA AND KARANGASEM REGENCIES, BALI Fakhrurrasi Fakhri; Inna Narayani; I G. Ngurah Kade Mahardika
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui keragaman genetik ikan cakalang (Katsuwonus pelamis) yang ada di Kabupaten Jembrana dan Karangasem, Bali. Sampel DNA diambil dari 30 ekor di kabupaten Jembrana dan 30 ekor di kabupaten Karangasem. Ekstraksi DNA dilakukan dengan menggunakan chelex 10% dan amplifikasi menggunakan metode PCR dengan konfigurasi HotStart pada control region DNA mitokondria, menggunakan primer forward CRK dan primer reverse CRE. Analisis filogenetika dilakukan dengan menggunakan metode Neighbor Joining dengan model Kimura 2-parameter. Variasi dari setiap sekuen diperoleh dengan menghitung jumlah, persentase dari tiap haplotipe, keragaman haplotipe (hd) dan keragaman nukleotida (?) menggunakan program DnaSP 5.10. Analisis Fst (Fixation Index) berdasarkan frekuensi haplotipe menggunakan Arlequin 3.1. Haplotipe pada sampel Jembrana berjumlah 28 haplotipe dan sampel Karangasem berjumlah 15 haplotipe dengannilai hd secara keseluruhan yaitu 0,997±0,006 dan untuk keragaman nukleotida (?) sebesar 0,04694. Nilai Fst yang diperoleh yaitu -0,00334 dan Fst P sebesar 0,90918±0,0100, menunjukkan bahwa populasi ikan cakalang di Kabupaten Jembrana dan Karangasem berasal dari populasi induk yang sama.
PHYSICAL, CHEMICAL AND BIOLOGICAL POTENTIAL HAZARDS IDENTIFICATION OF SLAUGHTERHOUSE AND CHICKEN NUGGETS PRODUCTION PROCESS Raynard c Sanito
Jurnal Biologi Udayana Vol 22 No 1 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.297 KB) | DOI: 10.24843/JBIOUNUD.2018.v22.i01.p04

Abstract

ABSTRACTChicken nuggets, the one of protein rich foods, is produced from the chicken meat through several stages ofthe process. This research aimed to identify the potential hazards of chicken nuggets process in final productsbased on physical, chemical and biological hazards perspectives. The hazardous potentials were performedspecifically using observations, interviews and investigations in a slaughterhouse unit and production unit.Furthermore, the finding of data was described qualitatively. The results of this study indicated that there wereseveral potential hazards in physical, chemical and biological in a chicken nugget process. The ear problems,exhausted of workers, watered by heat frying oil were the examples of potential hazards in physical. In addition,contamination of heavy metal in product and the expired of food grade oil were an illustration of chemicalpotential hazards. Then, microorganisms in end products, the source of the pathogen were the examples ofbiological hazards potential. Finally, it could be concluded that chicken nugget factory possessed the physical,chemical, and biological potential hazards.Key words: observations, interviews, findings, microorganisms.
KADAR KOLAGEN KULIT DAN TULANG PADA TIKUS BETINA USIA ENAM DAN 12 BULAN YANG DISUPLEMENTASI DENGAN SOMATOTROPIN Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The objective of this study is to investigate the effects of somatotropin supplementation on skin and bone collagen concentration of six-month and one-year old female mice. Forty eight female rats were assigned into a randomized block design with 4 factors. The first factor was age with 2 levels (6 months and 12 months). The second factor was somatotropin dosage with 2 levels (0 and 9 mg/kg body weight). The third factor was duration of injection with 2 levels (3 weeks and 6 weeks), and the fourth factor was sampling period with 2 levels (0 and 14 days after somatotropin termination). The results showed that somatotropin supplementation had significant effects on bone (P
VARIASI GENETIK MASYARAKAT SOROH PANDE DI KABUPATEN GIANYAR BERDASARKAN TIGA LOKUS DNA MIKROSATELIT AUTOSOM Gusti Ayu Putri Arnila; I Ketut Junitha; Made Pharmawati
Jurnal Biologi Udayana Vol 20 No 1 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Perkembangan ilmu forensik berkaitan erat dengan penggunaan analisa DNA mikrosatelit sebagai salah satu sarana yang paling akurat untuk mengungkap identitas seseorang. Penelitian ini dilaksanakan untuk mengetahuiragam alel yang tersebar pada masyarakat soroh Pande di Kabupaten Gianyar dengan menggunakan tiga lokus DNA mikrosatelit autosom yaitu D2S1338, D13S317 dan D16S539.  Berdasarkan hasil analisa ditemukan 23 ragam alel dengan rata-rata 7,3 per lokus. Rata-rata nilai heterozigositas dari tiga lokus yang digunakan adalah 0,817 ± 0,029 menunjukkan bahwa secara genetik masyarakat soroh Pande sangat bervariasi. Rata-rata nilai Power of Discrimination adalah 0,893. Tingginya nilai Power of Discrimination menunjukkan bahwa ketiga lokus ini baik digunakan untuk membedakan antar individu pada kepentingan forensik.
ISOLASI DAN IDENTIFIKASI JAMUR PENYEBAB PENYAKIT LAYU DAN ANTAGONISNYA PADA TANAMAN KENTANG YANG DIBUDIDAYAKAN DI BEDUGUL, BALI Ida Ayu Putu Suryanti; Yan Ramona; Meitini W. Proborini
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui jamur penyebab penyakit layu pada tanaman kentang dan mengisolasi antagonisnya dari daerah rhizosphere tanaman tersebut yang dibudidayakan di Desa Candikuning, Bedugul, Bali. Untuk mendeteksi patogen penyebab penyakit tersebut, pada penelitian diterapkan Postulat Koch. Hasil penelitian menunjukkan bahwa Fusarium oxysporum isolat A dan B merupakan dua isolat yang terindikasi sebagai penyebab penyakit layu pada tanaman kentang. Pada penelitian ini tiga isolat jamur antagonis (Trichoderma spp. isolat A, Trichoderma spp. isolat B, dan Aspergillus niger) berhasil diisolasi dari daerah rhizosphere tanaman kentang. Uji antagonis (in vitro) dengan menggunakan dual culture assay menunjukkan semua antagonis menghambat pertumbuhan jamur patogen Fusarium dengan persentase hambatan yang bervariasi antara 36,57 - 75,76%. Trichoderma spp. isolat A menunjukkan hasil terbaik dalam menghambat pertumbuhan jamur patogen, dengan persentase hambatan sebesar 75,76%. Sementara itu, persentase hambatan terkecil (36,57%) pada patogen teramati pada Aspergillus niger.
PANJANG SIKLUS ESTRUS MENCIT (Mus musculus L.) YANG DIBERI PEMANIS BUATAN ASPARTAM SECARA ORAL Sri Sulastri; Ngurah Intan Wiratmini; Ni Luh Suriani
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian aspartam terhadap panjang siklus estrus mencit betina dewasa. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 6 ulangan. Perlakuan P0 sebagai kontrol diberi aquades dan perlakuan P1, P2 dan P3 diberi aspartam dosis10 mg/kg bb; 15 mg/kg bb dan20 mg/kg bb. Aspartam diberikan setiap hari secara oral (gavage) selama 14 hari sebanyak 0,3 ml. Setelah 14 hari, apusan vagina dibuat setiap 8 jam dalam sehari selama dua kali siklus estrus. Variabel yang diamati adalah panjang waktu tiap fase dalam siklus estrus. Hasil analisis menggunakan Uji One Way Anova dan Uji Kruskal Wallis menunjukkan bahwa aspartam secara nyata (P<0,05) memperpanjang siklus estrus dengan peningkatan dosis yang diberikan.
MORPHOLOGICAL CHARACTERS OF CHILLI PEPPER (Capsicum frutescens L.) AS INFLUENCED BY SODIUM AZIDE AT GENERATIVE STAGE OF M1 GENERATION I Gusti Agung Eka Saraswati; Made Pharmawati; I Ketut Junitha
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Chili pepper (Capsicum frutescens L.) is one vegetable crop that has high economic value.  There are several problems in cultivation of chili pepper, for examples limited land, bad weather, and pests and diseases, which result in low production. These problems can be solved by developing new cultivar which has superior quality.  New cultivars of chili pepper plants can be obtained through induced mutation using chemical mutagen. In this research sodium azide (NaN3) was used as mutagen in chili pepper in order to obtain genetic variations that are useful in  increasing its  production.  The research was designed in  randomized  block  design. The observations were made on the response of plant morphology at generative stage, including plant height, number of branch, number of leaf, leaf length and leaf width.  The results showed that sodium azide increased plant height, branch number, leaf number and leaf length.  Sodium azide at concentrations of 2 mM and 5 mM were most effective in increasing plant height, number of leaves and number of branches. Keywords: Capsicum frutescens L., morphology, mutation, sodium azide
SUGAR BIOSYNTHESIS IN VANILLA PLANTS AND PHALAENOPSIS SEEDLINGS AFTER APPLICATION OF FERTILIZERS I Gede Ketut Adiputra
Jurnal Biologi Udayana Vol 21 No 2 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.428 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i02.p01

Abstract

In tropical country, vanilla encounters two main environmental conditions, wet and dry seasons. In the wet seasons when the amount of water available favours high growth rate, plants acquire more nutrients. However during dry seasons when water uptake via the root is limited and water loss from the shoot is increasing, vanilla will experience severe desiccations. In other cases, under condition of culture in bottle, phalaenopsis seedlings develop a very thin cuticle layers. These seedlings will experience a high rate of water loss when it is then transferred into ex vitro environmental conditions. Since mineral nutrient could decrease water potential in plants and enhanced by water loss, application of proper fertilizer into these vanilla plants and phalaenopsis seedling should not enhance the decreasing of water potential which could impair physiological mechanism. So, in order to ascertain nutrient requirement for these plants, two separate experiments were conducted. In the first experiment, vanilla plants grown in plantation were added 9.25, 1.1 and 4.6 g/plant Urea, TSP and KCL respectively. This experiment was repeated using different plants with application of fertilizer in higher doses; 18.5, 2.2 and 9.2 g/plant for Urea, TSPand KCl, respectively. In order to examine the capacity of these plants to synthesize sugar, samples were then harvested in the period after fertilizer application, i.e. at day 0, 11 and 24. In the second experiments, phalaenopsis seedlings were sprayed every 1-2 days with liquinox and rosasol-N fertilizer using factorial experimental design.. In order to examine the effect of the fertilizer on sugar biosynthesis, leaves of these plants were harvested at day 132. These experiments showed that addition of fertilizer into vanilla plants increased sugar content but addition of fertilizer into phalaenopsis seedling decreased sugar content. It is concluded that fertilizer applied into plants undergoing desiccation impair sugar biosynthesis.
VIABILITAS SPERMATOZOA PETAURUS BREVICEPS PAPUANUS T. NI MADE RAI SUARNI; I GUSTI AYU MANIK ERMAYANTI
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Experiment to determine the spermatozoa viability of Petaurus berviceps papuanus T. has been performed. Observation was done under microscope and trypan blue was used as stain. Viable spermatozoa were counted from a total of 100 spermatozoa. It was found that 4,75 % of the spermazoa could be maintained for 7.5 hours in the DMEM (Dulbecco's modified Eagle's medium) at 35oC.

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