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INDONESIA
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Published by Universitas Lampung
ISSN : 14117525     EISSN : 24610399     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.
Arjuna Subject : -
Articles 521 Documents
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CHARACTERIZATION OF EGGPLANT ENDOPHYTE BACTERIA AND RHIZOBACTERIA AS WELL AS THEIR ANTAGONISTIC ABILITY AGAINST Ralstonia solanacearum Saridewi, Larasati Puspita; Prihatiningsih, Nur; Djatmiko, Heru Adi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.220150-156

Abstract

Characterization of eggplant endophyte bacteria and rhizobacteria as well as their antagonistic ability against Ralstoniasolanacearum. Bacterial wilt caused by Ralsonia solanacearum is one of important diseases causing severe loses in eggplantproduction. Various strategies were used to manage bacterial wilt, including planting resistant varieties, soil amandement, andsoil solarization. However, management of R. solanacearum in eggplant by using endophytic bacteria and rhizobacteria werenot been done that much. The objective of this study was to: (1) characterization of endophytic and rhizobacteria; (2)determines the inhibition ability of endophytic and rhizobacteria isolates against R. solanacearum pathogen on eggplant. Thelaboratory experiment was arranged in completely randomized design with 5 treatments and 5 replications. The double layermethod using yeast peptone glucose agar (YPGA) medium was used in vitro test. Based on the morphological characteristicsthese isolates were suspected as a member of genus Bacillus. Among the isolates used in this study, TK isolate showed thebest capability to inhibit growth of R. solanacearum.
MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND Selangga, Dewa Gede Wiryangga; Listihani, Listihani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22197-102

Abstract

Molecular identification of Pepper yellow leaf curl Indonesia virus on chili pepper in Nusa Penida Island. Pepper yellow leaf curl Indonesia virus (PYLCV) has been reported as caused yellow leaf curl disease in Bali Island since early 2012. Dominant symptoms of PYLCV infection in chili pepper were yellowing, leaf curl, yellow mosaic, and mottle. Bemisia tabaci, has been known to vector on the case yellow leaf curl disease. Observations on the Nusa Penida Island in 2020 showed symptoms such as yellow leaf curl disease, however, identification of PYLCV in Nusa Penida Island has not been studied. Molecular identification was conducted using polymerase chain reaction and sequence analysis. Data collected in this study was disease symptoms and disease incidence. The results showed that dominant disease symptoms caused by virus from Nusa Penida were yellow mosaic, yellowing, and mottle. Universal DNA fragments of 912 bp were successfully amplified from 50 leaf samples using Begomovirus degenerate primers SPG 1 (5’-CCCCKGTGCGWRAATCCAT-3’) and SPG 2 (5’ATCCVAA YWTYCAGGGAGCT-3’). Sequence analysis showed that the isolate from Nusa Penida was a Pepper yellow leaf curl Indonesia virus with a 98–100% homology with several reference isolates.
NATURAL INFECTION OF Tobacco mosaic virus ON BUTTERNUT SQUASH IN BALI, INDONESIA Listihani, Listihani; Selangga , Dewa Gede Wiryangga; Sutrawati, Mimi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221116-122

Abstract

Natural infection of Tobacco mosaic virus on butternut squash in Bali, Indonesia. Tobacco mosaic virus (TMV) was a newly emerging virus infecting cucumbers in Indonesia since 2017. The mosaic disease caused by TMV potentially caused yield loss cucumber in Java. In 2019, mosaic symptoms were observed in butternut squash plants in Bali and molecular detection using universal primer of Tobamovirus indicated the presence of TMV infection. Further research was conducted to determine molecular characteristics of TMV on butternut squash plants in Bali. Specific DNA bands of Tobamovirus were amplified using reverse transcription polymerase chain reaction method, followed by DNA sequencing. The DNA were successfully amplified from CP Tobamovirus using universal primers from several butternut squash samples, i.e. Denpasar, Gianyar, Buleleng, and Karangasem Districts. The homology analysis of nucleotide and amino acid sequences of TMV among isolates from Denpasar, Gianyar, Buleleng, and Karangasem Districts was ranged between 95.6 – 97.7% and 98.1 – 99.4%, respectively. This indicated that low genetic diversity of TMV among Bali isolates. The highest homology of corresponding sequences of TMV isolates from Denpasar, Gianyar, Buleleng, and Karangasem Districts was closely related to TMV Kediri-Indonesia isolate on cucumber plant. Correspondingly, the phylogenetic analysis showed that TMV Bali isolates were categorized into same cluster with Kediri-Indonesia isolates. This was the first report of TMV on butternut squash in Indonesia.
COGONGRASS ROOT EXTRACT FROM FIVE DIFFERENT SOILS TYPES FOR SUPPRESSING PURPLE BLOTCH AND INCREASING GROWTH AND YIELD OF SHALLOTS Rokhlani, Rokhlani; Soesanto, Loekas; Nur, Subandi; Prihatiningsih, Nur
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221103-115

Abstract

Cogongrass root extract from five different soils types for suppressing purple blotch and increasing growth and yield of shallots. The aim of this study was to examine the efficacy of cogongrass (Imperata cylindrica (L.) Beauv.) root extracts from five different soil types (Typic Udipsamments, Aeric Endoaqualfs (=Gleisal Eutrik), Typic Quartzipsamments (=Regosol Eutrik), Aquertic Chromic Hapludalfs, and Pachic Hapludolls) in suppressing purple blotch and increasing growth and yield of shallots. Split plot design was used with 13 treatments repeated three times, and 18 plants plot-1. The treatments consisted of control, fungicide propineb applied before and after inoculation, and five types of cogongrass root extract 50, 60, and 70% concentration applied before and after inoculation. Results showed that cogongrass root extract collected from Pachic Hapludolls which was applied before inoculation had significant effect on the highest pathosystem component indicated by delaying the incubation period, suppressing the intensity of the disease, slowing down the infection rate, and decreasing values of AUDPC as 41.85, 69.87, 75.13, and 67.63%, respectively, compared to control. The cogongrass root extract from Pachic Hapludolls applied before inoculation could increase plant fresh and dry weight plant-1, tuber weight plant-1, plant fresh and dry weight plot-1, and tuber dry weight plot-1 as 42.7, 49.6, 51.92, 66.75, 72.29, and 73.53%, respectively, compared to control.
RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS Mokrani, Slimane; Nabti, El-hafid
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221123-133

Abstract

Rapid screening of phytopathogenic Erwinia sp. of two potato varieties (Spunta and Desiree) from Algerian agricultural fileds. Isolation, phenotypic identification and in vitro phytopathogenicity screening of Erwinia sp. from agricultural field of two potato varieties (Spunta and Desiree) in Algeria. The current study aims to isolate, identify and screen phytopathogenic isolates of Erwinia sp. causing potato diseases. The techniques presented in this study for isolation and characterization of phytopathogenic Erwinia sp. are conventional methods that are used in this field of research. Seven phytopathogenic bacteria were recovered from potato tubers of two varieties (Spunta and Desiree). The phenotypic identification allowed characterizing typical colonies of Erwinia sp. on two semi-selective media: King’s B and TCC media. Erwinia sp. formed characteristic colonies on King’s B medium that were round, convex and representing creamy color. While, Erwinia sp. also developed specific colonies on TCC medium which were pale purple, circular, convex, even bulging; smooth and mucous. In vitro phytopathogenicity test on potato slices lead to screen the phytopathogenic isolate E5 characterized by highest rotten tissue zone of (2.33 ± 0.29 cm) and (2.33 ± 0.58 cm) toward Spunta and Desiree varieties, respectively. Followed, by isolate E4 characterized by rotten tissue zone of (1.83 ± 0.58 cm) and (2.17 ± 0.29 cm) toward Spunta and Desiree varieties, respectively; compared to their corresponding uninfected controls. The RTZ (Rotten tissue zone) evidently is proportional to the specific pathogenicity of Erwinia sp. isolates and the characteristic sensitivity of various varieties (Spunta and Desiree). Thus, make determining RTZ a rapid screening technique for the selection of the highest phytopathogenic isolates. This investigation provides valuable information for rapid screening (infected potato tuber) and characterization (isolation using semi-selective media) of pathogenic Erwinia sp. engendering potato disease compared to existing methods like infection of leaves or plants; and phytopathogenic Erwinia sp. identification through PCR amplification or in situ hybridation.
DIVERSITY AND ABUNDANCE OF NEMATODES IN GUAVA (Psidium guajava L.) CULTIVATION IN LAMPUNG Nabilah, Nabilah; Swibawa, I Gede; Suharjo, Radix; Fitriana, Yuyun
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221134-143

Abstract

Diversity and abundance of nematodes in guava (Psidium guajava L.) cultivation in Lampung. Crystal guava agroecosystem is inhabited by many species of plant parasitic nematodes. However, information regarding this topic was still limited. This study aimed to understand the species dominancy of nematodes in crystal guava cultivation in Lampung. Sampling was carried out in three locations of guava crystal plantations: Lampung Timur, Lampung Tengah, and Tanggamus. The laboratory analysis was done at the Plant Pest Science Laboratory and Agricultural Biotechnology Laboratory, Universitas Lampung. The study was conducted in December 2019 – July 2020. Nematodes were identified to the level of the genus. The Prominance value (PV) was used to assess the nematodes genus dominancy. The results showed that the nematodes inhabiting the crystal guava agro-ecosystem in Lampung was both plant parasitic and free-living nematodes. The plant parasitic nematodes were identified as Meloidogyne, Aphelenchus, Hemicriconemoides, Tylenchus, Aphelenchoides, and Xiphinema, while free-living nematodes was Rhabditis, Dorylaimine, Dorylaimus, and Mononchus. The dominant plant parasitic nematode was Meloidogyne and the dominant free-living nematode was Rhabditis. The abundance of Meloidogyne /300 mL of soil was 351.47 individuals in Lampung Timur, 124.27 individuals in Lampung Tengah, and 82.18 individuals in Tanggamus. The dominant free-living nematode in the three locations was Rhabditis.
RESPONSE OF SIX CHILI VARIETIES TO ANTHRACNOSE DISEASE CAUSED BY Colletotrichum acutatum AND C. gloeosporioides Perdani, Ambar Yuswi; Paradisa, Yashanti Berlinda; Wahyuni, Wahyuni; Indrayani, Sri; Sulistyowati, Yuli; Cahyani, Yani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221144-150

Abstract

Response of six chili varieties to anthracnose disease caused by Colletotrichum acutatum and C. gloeosporioides. Chili is one of the horticultural commodities with high economic value. Chili production is constrained by anthracnose diseases. Losses due to anthracnose can reduce the fruits quality and yields lose. This study aims to determine the resistance of several chili varieties to anthracnose. Genetic material was used six varieties of chili. Ripe chilies were inoculated with two types of Colletotrichum isolates, i.e. C. acutatum and C. gleosporides. The experiment was arranged in a factorial randomized block design with three replications. The first factor were chili varieties: Laris, SSP, Habanero, Cibinong, Ekasari, and Kopay. The second factor was two types of fungal isolates. Observations were made on the incidence and severity of disease due to anthracnose. The results showed that both fungal isolates were effective in causing anthracnose disease in chilies. Habanero was very susceptible to anthracnose. Laris and Ekasari were moderately resistant to anthracnose diseases. These findings are important to develop new Capsicum cultivars that are more adaptive to anthracnose disease.
DIVERSITY OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) ATTRACTED BY ME LURE IN CSC-BG GERMPLASM CARAMBOLA PLANTATION Riastiwi, Indira; Paradisa, Yashanti Berlinda; Mambrasar, Yasper Michael; Raunsai, Marlin Megalestin; Perwitasari, Urip; Volkandari, Slamet Diah; Sari, Nurul Fitri; Sulistiyani, Tri Ratna; Ibo, Leberina Kristina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221151-157

Abstract

Diversity of fruit flies (Diptera: tephritidae) attracted by me lure in CSG-BG germplasm carambolae plantation. Germplasm Garden (KPN) Cibinong Science Center-Botanical Garden (CSC-BG) is one of the gardens that conserve and utilizes Indonesian local germplasm, one of which is carambolae. The current problem in KPN is the attack of fruit flies (Bactrocera spp.). This study was conducted to determine the diversity of fruit flies in the KPN carambola plantation area. A total of eight traps were placed randomly in the plantation area. Each trap contained 1 mL of methyl eugenol pheromone solution. Identification of fruit flies was carried out based on three morphological differences, they were wings, abdomen, and hind legs. There were 317 fruit flies collected and separated into three species, namely Bactrocera carambolae, B. papayae, dan B. umbrosa, of which B. carambolae had a dominance index of 0.74 and abundance index of 85%. Data on the types of fruit flies can be used as primary data to determine the preparation of pest prevention efforts.
EFFECT OF HOST-LARVAL DIET ON THE HOST ACCEPTANCE AND HOST SUITABILITY OF THE EGG PARASITOID Telenomus remus NIXON (Hymenoptera: Scelionidae) ON Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) Sari, Adha; Buchori, Damayanti; Nurkomar, Ihsan
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221158-165

Abstract

Effect of host-larval diet on the host acceptance and host suitability of the egg parasitoid Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). The life history of parasitoids is an important factor that can determine their ability to attack a host. The type of food consumed by the host can affect the ability of parasitoids such as host searching behavior, host suitability and host acceptance. In this research, we evaluate the effect of the S. frugiperda larvae diet on its suitability of the eggs produced by the adults for the egg parasitoid Telenomus remus. The research was studied on two types of egg masses of S. frugiperda that obtained from the moths that fed with natural or artificial diet during their larval stages. Parasitoid was reared from both types of hosts. An egg mass consisting of 50 S. frugiperda eggs from both types of hosts was exposed to one egg parasitoid female for 24 hours. S. frugiperda eggs then were reared until the parasitoid adult emerged. Each experiment was repeated 20 times. Host acceptance was observed through the host parasitism rate and its parasitization. Meanwhile, the host suitability was observed through the sex ratio of the emerging parasitoids. The results showed that S. frugiperda eggs reared using artificial diet had a higher parasitism rate (99.33%) than those of natural diet (82.53%). In contrast, the level of parasitization of S. frugiperda eggs reared using natural diet was higher (78.30%) than those of artificial diet (48.34%) because the number of emerging T. remus from S. frugiperda eggs reared using natural diet was also higher than those of artificial diet. However, the sex ratio (F:M) of emerging T. remus from S. frugiperda eggs reared using both of diet was female biased.
The effect of methyl eugenol from Ocimum minimum on the sticky trap to the direction and daily activity of fruit flies (Bactrocera spp.) Agus Kardinan; Paramita Maris
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12216-22

Abstract

The fruit fly Bactrocera spp. is a major pest in the horticulture. It caused several important commodities to rot and fall which lead into large loss, or even crop failure. The research objective was to obtain information regarding the activity of fruit fly during night and day and the most attractive direction of sticky trap to trap the fruit flies. The research consisted of 3 activities, namely (1) analysis of active ingredient of Ocimum plant, (2) study on the effect of ocimum sticky traps on the direction of fruit flies, and (3) study on the effect ocimum sticky traps on the daily activity of fruit flies. The study on the effect of time and direction of trap consisted of 4 treatments (North, South, East and West) and 6 replications. A sticky trap contained methyl eugenol as active ingredient, smeared on a yellow plastic surface measuring 10 × 20 cm and attached to 2 m high wooden poles, each facing East and West, as well as North and South (as opposed to one wood). Observations were made every hour starting from 03:00 am to 07:00 pm (until there was no fruit flies get trapped in each treatment). The results showed that sticky trap used contain Ocimum oil containing 76% of methyl eugenol used as an attractant for fruit flies. Fruit flies active during the day where the highest/peak activity of the fruit flies population occured at around 07:00 am. The direction of trap to East and West catched more fruit flies number than to North and South.

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