Article Per Year (5 Year)
p-Index From 2021 - 2026
2.737
P-Index
This Author published in this journals
All Journal Jurnal Riset Kesehatan Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Manuju : Malahayati Nursing Journal JURNAL PENELITIAN KESEHATAN Medicra (Journal of Medical Laboratory Science/Technology) Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal) Jurnal Pengabdian Kesehatan Beguai Jejama
Endah Astuti, Sri Sulami
Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 22 Documents
Search

 1   2   3 
Pemanfaatan Teri Jengki (Stolephorus indicus) Dan Layang Deles (Decapterus macrosoma) Sebagai Media Alternatif Pertumbuhan Escherichia coli Nurhasanah, Armelia Gitasari; Pestariati, Pestariati; Anggraini, Anita Dwi; Astuti, Sri Sulami Endah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14452

Abstract

ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar
Detection of The Blaz Gene as a Penicillin Resistance Marker in Staphylococcus Aureus From Diabetic Ulcer Swabs Indrayani, Salsabila Anggia; Pestariati, Pestariati; Astuti, Sri Sulami Endah
Jurnal Riset Kesehatan Vol 14 No 2 (2025): NOVEMBER 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v14i2.13592

Abstract

Diabetic ulcer is the most common complication in patients with diabetes mellitus, and Staphylococcus aureus is the predominant bacterial cause of infection. The aim of this study was to detect the presence of the blaZ gene as a penicillin-resistance marker in S. aureus isolated from diabetic ulcer swabs. This research was a descriptive quantitative study. The study population consisted of patients with diabetic ulcers treated at the Diabetes Wound Care Center Branch in Surabaya. Respondents (samples) were selected using purposive sampling, with inclusion criteria being patients diagnosed with diabetic ulcers and eligible for swab collection. A total of 30 ulcer swab samples were obtained between April and May 2025. Isolation and identification were conducted at the Bacteriology Laboratory of Poltekkes Surabaya, resulting in 16 isolates (54%) identified as S. aureus. Among these, 10 isolates (62.5%) were resistant to penicillin G. Detection of the blaZ gene using Real-Time PCR at the Molecular Biology Laboratory of Poltekkes Surabaya showed that 8 isolates (80%) were positive for the gene. These findings indicate that most penicillin-resistant S. aureus isolates carry the blaZ gene, suggesting high potential for β-lactamase production. Future studies are recommended to include larger sample sizes, analyze additional resistance genes, and investigate clinical factors influencing the emergence of antibiotic resistance in diabetic ulcers.
Co-Authors ., Pestariati Anggraini, Anita Dwi Anita Dwi Anggraini Anita Dwi Anggraini Armelia Gitasari Nurhasanah Atsania Putri , Puji Lestari Candra Triana Hikma Choirudin, Nadia Pramesthy Choirudin Diah Titik Mutiarawati Edy Haryanto Edy Haryanto Edy Haryanto Endarini, Lully Hanni Evy Diah Woelansari Fitria Yulfirda Arini Handayati, Anik Hikma , Candra Triana Ida Lestari Indrayani, Salsabila Anggia Jihan Azzahra Arsyi Juliana Christyaningsih Kartika Rahayuningsih, Christ Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nurhasanah, Armelia Gitasari Puji Lestari Atsania Putri Putri, Ervina Wahyu Putri, Tiara Dewanti Retno Sasongkowati Sasongkowati, Retno Sasongkowati, Retno Sri Utami Suhariyadi Suhariyadi Suliati Suliati Syamsul Arifin Syamsul Arifin Wisnu Istanto