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IDENTIFICATION, ISOLATION, PURIFICATION, GRAM STAINING, AND BIOCHEMICHAL TESTING PROCESSES OF AQUATIC BACTERIA FROM DIFERENT SOURCES Pardede, Maria Agustina; Fasya, Arif Habib; Kumalaningrum, Dwi Retna; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Ambarwati, Dewi; Maulana, Muhammad Hilmy
Jurnal Segara Vol 19, No 3 (2024): December
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/segara.v19i3.15409

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Penerapan Budidaya Ikan Lele Intensif Sistem Aquaponik sebagai Upaya Peningkatan Produksi Ikan pada Kelompok Pembudidaya Ikan di Kabupaten Banyuwangi Prayogo; Fasya, Arif Habib; Maulana, Muhammad Hilmy; Prayogo, Andhika Mufid
Jurnal Pengabdian Kepada Masyarakat Patikala Vol. 5 No. 2 (2025): Jurnal PkM PATIKALA
Publisher : Pusat Pengembangan Pendidikan dan Bakat Indonesia/Education and Talent Development Center of Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51574/patikala.v5i2.4120

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The fisheries potential of Banyuwangi Regency is substantial due to its abundant natural resources. The Fish Farmers Group (Pokdakan) Sumber Bening serves as a productive partner located in Rejopuro Hamlet, Kampunganyar Village, Glagah District, Banyuwangi Regency, an area situated within a watershed (DAS) that supports aquaculture activities. This Community Service Program (Pengmas) aims to empower the Sumber Bening fish farmers group as a productive partner; however, they face challenges related to low production output. Initial identification revealed two main problems: limited human resource capacity in intensive catfish cultivation and weak technical management. The interventions implemented included the establishment of an aquaponic cultivation demonstration plot, technical training sessions, as well as continuous mentoring and evaluation. The results showed a significant improvement in skills and competencies—from 8% before training to 75% after training—along with an increase in motivation to develop from 10% to 80%. The final capacity improvement reached 100% in both indicators, indicating successful technology transfer and adoption of intensive aquaculture practices. The partner group successfully implemented Standard Operating Procedures (SOPs) for cultivation, conducted daily record-keeping, and achieved improved feed efficiency, reduced mortality rates, and produced additional outputs in the form of organic vegetables. The growth performance indicated a Feeding Rate (FR) of 1:1 and a Survival Rate (SR) of 99%. This program resulted in the development of a model fish farming group that is adaptive and has strong potential for replication in other regions.
Economic improvement and fish farming based on Smart Aquaculture Automatized System in Segobang Village, Banyuwangi Arif Habib Fasya; Hapsari Kenconojati; Darmawan Setia Budi; Suciyono Suciyono; Maria Agustina Pardede; Dwi Retna Kumalaningrum; Prayogo Prayogo; Eka Saputra; Aji Akbar Firdaus; Muhammad Hilmy Maulana; Dewi Ambarwati
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 10 No. 1 (2025): February 2025
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v10i1.14811

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The fisheries and marine sector in East Java has abundant potential, one of which is Banyuwangi district. Banyuwangi Regency has potential in freshwater aquaculture because it has 324 rivers with an area of 735 km2 and swamps and reservoirs of 60 hectares. One of the villages in Banyuwangi Regency is Segobang Village, which has the potential to have abundant water sources and continuous flow. This potential can be utilized by training and assisting fish nurseries in Segobang Village by utilizing continuously flowing water and Smart Aquaculture Automatized System (SAAS) technology by utilizing biofilters and running water and aeration as a source of oxygen.  The stages carried out in this community service are as follows: initial assessment of the location, infrastructure and technology development, periodic training and mentoring, implementation and monitoring of the cultivation process, and continued marketing and sales. After the training, the Segobang Village community showed a significant increase in knowledge. The average pre-test scores ranged from 30 to 51, while the post-test scores increased to 79 to 87. This improvement shows that participants better understand the SAAS and its use in fish farming. Continued training and practice can help strengthen the adoption of this technology in the community.
Comparison of the number and distribution of chromosomes of three varieties of Nile tilapia (Oreochromis niloticus) Darmawan Setia Budi; Lailatul Lutfiyah; Arif Habib Fasya; Prayogo Prayogo
Journal of Aquaculture Science Vol 4 No 1 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i1.66

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The aims of this study is to determine the number and distribution of the chromosomes of three Nile tilapia (Oreochromis niloticus) varieties as basic information in the process of identifying the characteristics of varieties in tilapia. This research was conducted in August-October 2016, at the Unair Banyuwangi PSDKU laboratory. The research procedures included rearing of test fish, immersion of test fish with colchicine solution and tissue preservation, preparation of preparations and staining, and observation. Based on the results of the study, it was found that each variety of tilapia (black, red, and white) showed a difference in the spread of chromosomes, while the number of chromosomes was the same, namely 44 pieces.
Relation between age and fecundity of giant gouramy (Osphronemus gouramy) Arif Habib Fasya; Hilda Nabila
Journal of Aquaculture Science Vol 5 No 1 (2020): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v5i1.85

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The need for gouramy (Osphronemus gouramy) continues to increase, this can be seen from the gouramy trade which has begun at the egg stage, so research is needed that affects the number of eggs (fecundity) of the gouramy parent. In this study aims to determine the relationship between age and fecundity of gouramy. The research method used is comparative descriptive. Main gouramy fish is divided into 3 based on their age, pond A is 2.5 years old, pond B is 4 years old and pond C is 8 years old. Observations were made by taking eggs from the nest in each pond then counted manually. The observations showed that the parent who was 4 years old had the highest level of fecundity. Keyword : Age, Fecundity, Osphoronemus gourami
Effect of Different Culture Containers to Cell Densities of Nitzschia in Laboratory Scale Mohammad Faizal Ulkhaq; Yeni Dwi Kharismawati; Arif Habib Fasya
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.77

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The study aimed to determine the type of culture container for increasing the cell density of Nitzschia and producing the hightly cell biomass. The stages of this study were sterilization of containers and media, making diatom fertilizers and agar media, culture of Nitzschia in agar medium, culture of Nitzschia in test tube, and culture of Nitzschia in glass and plastic containers. The result showed that the cell density of Nitzschia cultured in the glass container was higher than plastic containers. Further study was needed to determine the nutrient composition of Nitzschia that culture in glass containers.Keyword : Nitzschia culture, Laboratory scale, Glass, Plastic container.
Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Aulia Azizah; Darmawan Setia Budi; Arif Habib Fasya; Muhammad Hanif Azhar; Hapsari Kenconojati
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.71

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Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions.   Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus. 
The Separating Technique of Abalone (Haliotis squamata) at Marine Aquaculture Development Centre (MADC) Lombok, West Nusa Tenggara Province arif habib fasya; Mohammad Faizal Ulkhaq; Ade Prasetyo Abdi
Journal of Aquaculture Science Vol 6 No 1IS (1): Vol 6 Issue Spesial 2021 Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i1IS.158

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The purpose of this research is to find out the abalone separating technique (H. squamata) and know the problems during separating at the Marine Aquaculture Development Centre, West Nusa Tenggara Province. Research are carried out on December 23, 2019 to January 21, 2020. The method used is descriptive method with data collection including primary data and secondary data. Data is taken by observing, active participation, interviewing, and literature review. Abalone separating techniques (H. squamata) begin with container preparation, seed preparation, seed stocking, seeding, feeding, water quality management, and growth sampling. Problems encountered during the separating stage are the uncertain availability of seaweed feed. Keyword :  Abalone, H. squamata, separating technique.
Hubungan Panjang dan Berat terhadap Fekunditas Ikan Cupang (Betta sp.) Arif Habib Fasya; Mufidah F
Journal of Aquaculture Science Vol 7 No 2 (2022): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v7i2.257

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Betta fish is a fish that has a high economic value, Market demand always increasing. However, to get high Quality betta fish, efforts are still needed by importing from Singapore and Thailand so that a sustainable betta fish hatchery business is needed. To develop this betta fish hatchery business, the first thing that must be considered is the value of fecundity. Fecundity plays an important role in supporting the success of fish growth. This study aims to determine the relationship between the length and weight of betta fish broodstock to the value of fecundity as an effort to develop in the field of aquaculture, especially at the hatchery stage. The method used in this research is Descriptive method which is then analyzed using linear regression, namely the relationship between length and weight on the fecundity of betta fish. The data is presented in the form of tables and graphs which are then analyzed descriptively to draw conclusions from the resulting data. In this study, 30 brood betta fish were used with body weights ranging from 1.39 to 1.73gr and lengths of 4.5 to 5.2cm and the resulting fecundity ranged from 166 to 366 eggs. The results showed that fecundity had a close relationship with fish body weight where the correlation coefficient (r) was 0.9653 and the coefficient of determination (R²) was 0.9319. While the long relationship with fecundity has a moderate relationship where the correlation coefficient (r) obtained is 0.4079 and the coefficient of determination (R²) is 0.1664. This indicates that fish weight is better used to estimate the value of fish fecundity than body length. Keyword: Betta fish, Fecundity, Body weight, Body length
The Characterization of Gold Nanoparticle (NP-AU) Biosynthesis with Bioreductor Chaetoceros calcitrans as a Disease Inhibitory Power of AHPND in Vannamei Shrimp (Litopenaeus vannamei) : Bahasa Indonesia Diva Desmieta; Sofiya Amalina; Dhea Puspita Hakim; Isac Muhamad Kahlil Gibran; Azizah Raihani Purnama; Prayogo; Arif Habib Fasya
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.61961

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Indonesia is the third largest global shrimp exporter after Ecuador and India. Shrimp production increased by 21% from 1.21 million tons to 1.48 million tons in 2021-2022, but decreased to 1.097 million tons in 2023. This is thought to be due to AHPND (Acute Hepatopancreatic Necrosis Disease). Our research aims to determine the biofabrication, characterization and effectiveness of gold nanoparticle biofabrication mediated by Chaetoceros calcitrans as an antibacterial against AHPND in white shrimp. The method used is experimental. The findings were the antibacterial potential of gold nanoparticle biofabrication mediated by C. calcitrans as an AHPND drug in vaname shrimp. Based on the research results, biofabrication was successfully formed with UV-vis characterization at a wavelength of 533 nm, in the FTIR test the AU3+ group was successfully reduced by the bioactive compound C. calcitrans. The results of the SEM and TEM tests showed that nanoparticles were dominated by a spherical shape, the particle size according to the PSA test was 8.25 nm and the XRD test answered that Np-Au crystallization had formed with the appearance of a high intensity peak.