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All Journal Indonesian Journal of Tropical and Infectious Disease Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Kesehatan Manarang GUIDENA: Jurnal Ilmu Pendidikan, Psikologi, Bimbingan dan Konseling Faletehan Health Journal Care : Jurnal Ilmiah Ilmu Kesehatan JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Clinical Microbiology and Infectious Diseases

Wardhani, Puspa

Unknown Affiliation

Author-ID : 1082155

Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Pengaruh Koherensi dan Fungsi Keluarga pada Kualitas Hidup saat Pandemi COVID-19 Azza, Irine Irchamillah; Asiyah, Siti Nur; Wardhani, Puspa
GUIDENA: Jurnal Ilmu Pendidikan, Psikologi, Bimbingan dan Konseling Vol 13, No 1 (2023)
Publisher : Universitas Muhammadiyah Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/gdn.v13i1.6392

Everyone, especially frontline workers, feels the impact of COVID-19. In dealing with COVID-19, the family has a role that can impact the quality of life of health workers and non-health workers during the COVID-19 pandemic; a good role for the family will result in better health services and increased work engagement. Research on workers in the non-health sector in pandemic conditions is still rare, making researchers choose to take two groups of subjects. It is hypothesized that there is an influence between family coherence and family functioning on the quality of life of the subjects, and there are differences between the two groups of subjects. This study's results indicate an influence between the family sense of coherence and family functioning on the quality of life of health workers and non-health workers during the COVID-19 pandemic (F= 38,978; p>0,05; adjusted R2=0,276). There is no difference in the level of quality of life between health workers and non-health workers (p=0,307; mean differences = 1,63; 95%CI[-1,52; 4,77]), but there were differences in other variables. The implication of this research is the need to strengthen the family base so it can become a source of coping for family members.

INHIBITORY ACTIVITY OF COBALT(II)–MORIN COMPLEX AGAINST THE REPLICATION OF DENGUE VIRUS TYPE 2 Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi Setyawati; Mulyatno, Kris Cahyo; Amarullah, Ilham Harlan; Ueda, Shuhai; Kotaki, Tomohiro; Sumarsih, Sri; Wardhani, Puspa; Bendryman, Sri Subekti; Aryati, Aryati; Soegijanto, Soegeng; Kameoka, Masanori
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin complex. This compound was further investigated for its inhibitory effect on the replication of DENV-2 in Vero cells. The replication of DENV was measured by enzyme-linked immunosorbent assay and the value of selectivity index (SI). SI was determined as the ratio of the 50% cytotoxic concentration (CC50) to the 50% inhibitory concentration (IC50). The IC50 value of the Cobalt(II)–Morin complex for DENV-2 was 3.08 µg/ml, and the CC50 value of the complex for Vero cells was 3.36 µg/ml; thus, the SI value was 1.09. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of Cobalt(II)–Morin complex and its high toxicity in Vero cells. Further studies are not required before Co(II)–Morin can be applied in the treatment of DENV-2 infections.

RNA ISOLATION OF DENGUE VIRUS TYPE 1 WITH DIFFERENT PRECIPITATION SOLVENTS: DIMETHYL SULFOXIDE, ACETONE, AND ETHANOL 70% Maharani, Anisa; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Ueda, Shuhai; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 3 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue Hemorrhagic Fever (DHF) is caused by dengue viruses that belong to Flaviviridae. The disease is known to be caused by 4 types of dengue viruses, namely DENV-1, DENV-2, DENV-3, and DENV-4 associated with antigenic. Dengue virus is a virus RNA that causes illness with clinical manifestations of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome. The aim of research was to determine the effectiveness of dimethyl sulfoxide, acetone, and ethanol 70% as precipitation solvent in the process of RNA isolation. The method used was Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) with specific primers for dengue virus type 1 (DENV-1). RNA isolation can be done easily using an RNA Isolation Kit. Use of RNA Isolation Kit results in a purer RNA isolate from contaminants and from RNA degradation. In generally the isolation is using cold ethanol / alcohol with concentration 90-95%. Ethanol / Alcohol does not dissolve RNA and light density of alcohol lighter than water makes RNA rise and hover on the surface. In RNA isolation solvent precipitation that used are acetone, ethanol 70%, and DMSO. In qualitative RNA measurements using agarose gel electrophoresis and was examined under the UV light-illuminator and quantitative RNA measurements using Nanodrop spectrophotometry with absorbance ratio at 260/280 and 260/230 showed a good result indicated by the appearance of the band on electrophoresis results in PCR. While the measurement quantitatively is showed that there was still protein contamination but the results are quite good because it does not much different from the ratio set in the reference. Acetone, ethanol 70%, and DMSO can be used as a substitute of 96% ethanol in the process of RNA isolation in DENV-1 virus and can also be applied to other dengue virus because the structure of the 4th antigen serotype is very similar one with the other and no effect.

ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Sumarsih, Sri; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 Î¼g/ml, 2.75 Î¼g/ml, respectively and 2.00 Î¼g/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 Î¼g/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 Î¼g/ml.

Eï¬€ect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell Sucipto, Teguh Hari; Wibrianto, Aswandi; Martak, Fahimah; Churrotin, Siti; Amarullah, Ilham Harlan; Setyawati, Harsasi; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 3 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i3.11776

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many eï¬€orts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory eï¬€ect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 Î¼g/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 Î¼g/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections

Effect of low voltage electric currents on the decrease of Klebsiella pneumoniae ESBL and non-ESBL colonies Setya Wijoyo, Dhia Lintang; Koendhori, Eko Budi; Susilo, Imam; Wardhani, Puspa
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 1 (2023): Available online : June 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i1.17

Introduction: Klebsiella pneumoniae is a gram-negative bacteria and one of the most common causes of nosocomial infections, especially in the intensive care units. The use of liberal and irrational antibiotics is shown the emergence of antibiotic-resistant Klebsiella pneumoniae. This research was conducted to evaluate if low voltage electric current on three kinds of solvent media could have an eradication effect. Methods: This was an experimental study, which was done at the Microbiology Laboratory of Harapan Kita Women and Children Hospital-Jakarta. This study used to isolate bacteria non-ESBL and ESBL Klebsiella pneumoniae, thereupon will be dissolved in saline, Aqua destillata, and Ringer Lactate, each consisting of 8 samples. Each sample received a 0.5V and 10mA DC electric current; reduction of colonies was observed at 30, 60, 120 and 240 minutes using DensiCHEK. Result: There was a decrease in the colony number of 2 bacterial groups in the first 30 minutes in all three media (p <0.01). The reduction was higher in the non-ESBL K. pneumoniae group. The decrease of bacterial colonies was higher in the Klebsiella pneumoniae group non ESBL in ringer lactate medium during 240 minutes observation compared to saline and Aqua destillata (p <0.001; p <0.001, respectively). The saline solution showed no different effect compared to aquadestillata. Conclusion: A direct current of 10 mA and 0.5 V intervention, on Ringer lactate media, could have a bacterial killing effect to Klebsiella pneumoniae non ESBL started from 30 minutes. Klebsiella pneumoniae ESBL needed a longer duration than non ESBL.

Urinalysis Profile of Dengue Viral Infection Patients in Dr. Soetomo General Academic Hospital, Surabaya, from 2019-2021 Rahardjo, Albertus Reiner; Wardhani, Puspa
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 14 No. 2 (2023): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V14I22023.94-99

Highlights:1. Dengue viral infection patients were mostly male and aged 17-45 years old.2. Dengue viral infection patients mostly have normal urinalysis results.3. Some dengue viral infection patients can have elevated protein, blood, bilirubin, urobilinogen, leukocytes esterase, and ketones in the urine. AbstractIntroduction: Dengue viral infection is still a burden in Indonesia. It often causes some kidney complications such as acute renal failure, proteinuria, hematuria, and glomerulonephritis. Urinalysis is an easy and useful examination of kidney complications. This study aimed to discover the most common profile of urinalysis in dengue viral infection. Methods: This study used a cross-sectional method with medical records data of patients with dengue viral infection in Dr. Soetomo General Academic Hospital, Surabaya, in the 2019-2021 period. Purposive sampling techniques were used with inclusion and exclusion criteria. Data such as age, gender, dengue viral infection severity level, and dipstick urinalysis results were taken and analyzed using descriptive analysis in SPSS Statistics 25.Results: A total of 276 patients were included. The sample was dominated by male patients (52.9%) and patients aged 17-45 years old (40.2%). Most patients suffered from dengue hemorrhagic fever/DHF (54.3%) followed by dengue fever (45.7%). DHF was categorized into grade I (28.3%), grade II (13.4%), grade III (8.3%), and grade IV (4.3%). Most urinalysis results were normal and followed by increased results of protein, blood/hemoglobin, bilirubin, urobilinogen, leukocytes esterase, and ketones in some patients.Conclusion: Dengue viral infection was more common in male patients and 17-45 years old patients. The most common diagnosis was DHF. Urinalysis results in dengue viral infection were mostly normal. Follow-up research is needed to find the relation between the elevated urinalysis results and the severity of dengue viral infection.

Penguatan Manajemen Laboratorium dalam Menurunkan Morbiditas dan Mortalitas Hepatitis B Putri, Aditea Etawati; Hernaningsih, Yetti; Wardhani, Puspa; Rini, Diah Puspita; Indrasari, Yulia Nadar; Ramadhan, Nanda Aulya
Faletehan Health Journal Vol 12 No 01 (2025): Faletehan Health Journal, Maret 2025
Publisher : Universitas Faletehan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Hepatitis B virus (HBV) infection is one of the threats in the medical world and requires guaranteed and valid laboratory analysis procedures, one of which is through the quality control (QC) mechanism. As part of laboratory management, QC has an impact on the validity of laboratory analysis results, especially related to HBV infection. The purpose of this study was to determine the level of knowledge of medical analysts before and after laboratory management training. The research method used comparative paired test analysis to obtain the significance and binary logistic regression to determine the most influential questions on the test. The results showed a significant increase in medical analyst knowledge after being given laboratory management material. Supported by a p value = 1.000 from the Hosmer and Lemeshow test and an overall prediction of 95.5% (positive: 92.0% and negative 97.6%), this training was very good in terms of goodness-of-fit and accurate. Material related to immunochromatography (ICT) method is an influential factor based on the analysis of test results. Medical analyst training, especially related to laboratory management in the context of HBV management, has a significant impact and can be continued and developed.

Correlation between Neutrophil-to-Lymphocyte Ratio and Severity of Community-Acquired Pneumonia in Patients Almira, Naufalia Ghina; Hasan, Helmia; Wardhani, Puspa; Hernaningsih, Yetti; Bintoro, Siprianus Ugroseno Yudho; Romadhon, Pradana Zaky
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 9 No 02 (2025): Qanun Medika Vol 09 No 02 July 2025
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v9i02.25024

The high morbidity and mortality rates due to pneumonia in developing countries indicate that a more affordable marker is needed to determine the degree of inflammation in cases of pneumonia infection. The neutrophil-to-lymphocyte ratio (NLR) is a simple indicator used extensively in evaluating the systemic inflammatory response. An observational cross-sectional study of 297 pneumonia patients was conducted using medical records as the source document. The patients’ blood was taken during the hospital admission to obtain neutrophil and lymphocyte values. Meanwhile, the PSI score was used during the hospital admission to assess the severity of the pneumonia. Bivariate analysis using Spearman correlation was used to determine the correlation between NLR and pneumonia severity. The ROC curve was used to determine the cut-off value of NLR to distinguish mild from severe patients. The results of the Spearman correlation test analysis showed that the correlation between the NLR and the pneumonia severity was significantly positive (r = 0.130; p = 0.025). Furthermore, the median NLR value was higher in patients with severe pneumonia than in those with milder pneumonia severity (13.01 vs. 11.79 vs. 6.75). The NLR cut-off value of 9.07 was obtained to differentiate patients with mild severity and those with more severe disease. In conclusion, there is a correlation between the neutrophil-to-lymphocyte ratio and the severity of patients’ pneumonia.

SOCIALIZATION OF CORRECT HANDWASHING STEPS TO CREATE A HEALTHY STUDENTS AT DENANYAR, JOMBANG, INDONESIA Hernaningsih, Yetti; Aulia, Fauqa Arinil; Rini, Rini; Wardhani, Puspa
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 7 No. 4 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i4.2023.574-580

Elementary school students in Indonesia generally do not know the proper and correct steps for washing hands according to the World Health Organization (WHO), which is indicated by the high rate of spread of hand-borne diseases, including diarrhea. Diarrhea is an endemic disease in Jombang Regency with the incidence rate increasing in 2019 compared to 2018. Teaching Staff of the Department of Clinical Pathology, Faculty of Medicine, UNAIR, carry out community service activities with the aim of providing education on how to wash hands properly and correctly for Madrasah Ibtidaiyah students (MI) Mambaul Maarif Denanyar Jombang. The method of socializing the correct hand washing steps is carried out in two stages, namely education and evaluation. The education carried out is an explanation of the correct steps for washing hands according to WHO, what germs are found on human hands, what diseases can be transmitted through hands, and when we should wash our hands. Evaluation is carried out by culturing germs from the surface of the hands at two times, namely before and after washing hands. The data obtained is the difference in bacterial culture results in specimens before and after washing hands. The growth of bacteria before hand washing showed more colonies compared to after hand washing.

Co-Authors Almira, Naufalia Ghina Aryati Aryati Aulia, Fauqa Arinil Azza, Irine Irchamillah Bintoro, Siprianus Ugroseno Yudho Diah Puspitarini Eko Budi Koendhori, Eko Budi Fahimah Martak Harsasi Setyawati Hasan, Helmia Hermina Novida, Hermina Ilham Harlan Amarullah Imam Susilo Indrasari, Yulia Nadar Kris Cahyo Mulyatno, Kris Cahyo Maharani, Anisa Masanori Kameoka, Masanori Nanda Aulya Ramadhan Putri, Aditea Etawati Putri, Anandia Nafisah Rahardjo, Albertus Reiner Ratri, Maya Masita RINI RINI Romadhon, Pradana Zaky Setya Wijoyo, Dhia Lintang Siti Churrotin, Siti Siti Nur Asiyah Soegeng Soegijanto Sri Subekti Sri Sumarsih Teguh Hari Sucipto, Teguh Hari Tomohiro Kotaki, Tomohiro Triyani, Irma Ueda, Shuhai Wibrianto, Aswandi Yetti Hernaningsih

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