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Histopathological evaluation of hepatic tissue of yellow Rasbora (Rasbora lateristriata) exposed to paracetamol Septriani, Nur Indah; Saribu, Ruth Liananda Citra Dolok; Apriliyani, Tia; Karlina, Ina; Pusparini, Nur Ainun Oktavia; Zusrina, Laili Mufli; Sari, Ria Vinola Septhya; Allimi, Hayu Swari; Supraitno, Murtiadi Erlan; Saeed, Faisal; Simanungkalit, Eben Ezer; Paramita, Pradnya; Retnoaji, Bambang; Sofyantoro, Fajar; Wijayanti, Nastiti
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.595

Excessive consumption of paracetamol may lead to hepatic necrosis, nephrotoxicity, extra-hepatic lesions, and even fatality in both humans and animals. The objective of this study was to examine the histological changes in the hepatocytes of yellow rasbora (Rasbora lateristriata) exposed to paracetamol. The fish were subjected to varying concentrations of paracetamol over a period of four days. Group I served as the control group without any paracetamol exposure. Groups II, III, and IV were exposed to 2, 3, and 4 mg/L of paracetamol, respectively. A total of six individuals were allocated to each of the four experimental groups. The histological analysis of the hepatic tissue following paracetamol exposure at concentrations of 2, 3, and 4 mg/L revealed hepatic damage characterized by picnosis, necrosis, and vacuolization. In summary, higher concentrations of paracetamol were associated with an increase in the severity of histopathological changes in the hepatic tissues of yellow rasbora.

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

Bridging Pedagogy and Technology: The Impact of Pancasila Project Understanding, Digital Literacy, and Teacher Competence on Digital Learning Content Creation Susanto, Prasetyo; Dominica Niron, Maria; Saeed, Faisal
Journal of Innovation and Research in Primary Education Vol. 4 No. 4 (2025)
Publisher : Papanda Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56916/jirpe.v4i4.2378

This study aims to examine the effects of understanding the Strengthening Pancasila Student Profile Project (P5), digital literacy, and pedagogical competence on teachers’ creation of digital learning content. A quantitative explanatory design was applied at an elementary school in Yogyakarta, Indonesia. Eighty-three teachers were selected using a simple random sampling technique. Data were collected through a structured questionnaire and analyzed using simple and multiple linear regressions. The findings reveal that understanding of P5 significantly influences digital content creation (β = 0.248), digital literacy emerges as the strongest predictor (β = 0.323), and pedagogical competence also shows a significant positive effect (β = 0.237). Collectively, the three variables explain 54.5% of the variance in teachers’ digital content creation (R² = 0.545). These results highlight the importance of integrating Pancasila values, digital literacy, and pedagogical skills to strengthen teachers’ capacity in implementing P5 through digital learning content. Future studies are recommended to explore supporting factors such as school leadership, infrastructure readiness, and teacher motivation.

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

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