Garuda - Garba Rujukan Digital

Histopathological evaluation of hepatic tissue of yellow Rasbora (Rasbora lateristriata) exposed to paracetamol Septriani, Nur Indah; Saribu, Ruth Liananda Citra Dolok; Apriliyani, Tia; Karlina, Ina; Pusparini, Nur Ainun Oktavia; Zusrina, Laili Mufli; Sari, Ria Vinola Septhya; Allimi, Hayu Swari; Supraitno, Murtiadi Erlan; Saeed, Faisal; Simanungkalit, Eben Ezer; Paramita, Pradnya; Retnoaji, Bambang; Sofyantoro, Fajar; Wijayanti, Nastiti
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.595

Excessive consumption of paracetamol may lead to hepatic necrosis, nephrotoxicity, extra-hepatic lesions, and even fatality in both humans and animals. The objective of this study was to examine the histological changes in the hepatocytes of yellow rasbora (Rasbora lateristriata) exposed to paracetamol. The fish were subjected to varying concentrations of paracetamol over a period of four days. Group I served as the control group without any paracetamol exposure. Groups II, III, and IV were exposed to 2, 3, and 4 mg/L of paracetamol, respectively. A total of six individuals were allocated to each of the four experimental groups. The histological analysis of the hepatic tissue following paracetamol exposure at concentrations of 2, 3, and 4 mg/L revealed hepatic damage characterized by picnosis, necrosis, and vacuolization. In summary, higher concentrations of paracetamol were associated with an increase in the severity of histopathological changes in the hepatic tissues of yellow rasbora.

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

MORPHOMETRY OF THE GILL AND ARBORESCENT STRUCTURES OF Clarias gariepinus (BURCHELL, 1822) AT DIFFERENT DEVELOPMENTAL STAGES Nuriliani, Ardaning; Karlina, Ina; Rohmah, Zuliyati; Sari, Dini Wahyu Kartika
Jurnal Penelitian Perikanan Indonesia Vol 31, No 2 (2025): (Juni 2025)
Publisher : Politeknik Kelautan dan Perikanan Karawang, BRSDM KP.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jppi.31.2.2025.%p

Catfish is one of Indonesia's superior fishery commodities. Catfish have an adaptation mechanism to an environment with minimal oxygen in their gill structure, namely arborescent to support their successful lives. This study examines Mutiara catfish's gill and arborescent morphometry at various developmental stages. The morphometric parameters consist of several parts, namely gill structure and arborescent structure. The gill structure includes the ratio of gill weight right and left to the weight and total length of the fish. Apart from that, the gills are divided into three main parts, namely the branchial arch, branchial filaments, and branchiospinalis. The part of the branchial arch observed includes the ratio of the length of the branchial arch to the total length of the fish. The branchial filament section includes the average number of branchial filaments per branchial arch, the average density of branchial filaments (filaments/cm), and the ratio of branchial filament length to branchial arch length. The branchiospinal section includes the average number of branchiospinalis per branchial arch and branchiospinal density. Arborescent structures include ratios of relative arborescent weight (%) right and left to total fish weight and the ratio of the number of arborescent branches to total arborescent weight. Catfish were sampled from the larval stage (1–14 days), juvenile (15–21 days), and post-juvenile to pre-adult (22–90 days). Statistical analysis of data using SPPS 22 software. Mutiara catfish in the post-adolescent to preadult stage have higher relative gill weight and relative arborescent weight than the juvenile and larval stages. The post-adolescent to preadult stage has a more developed gill and arborescent shape and size than the juvenile and larval stages.

Gambaran Penyakit Neurodegeneratif: Huntington, Alzheimer, dan Parkinson: Sebuah Tinjauan Karlina, Ina; Andriyani, Eka Fitri Siti; Pratiwi, Arini Dian; Prasasti, Filosofia Florista Tesla Aulia; Tunjung, Woro Anindito Sri; Rohmah, Zuliyati; Nuriliani, Ardaning
Jurnal Biomedika dan Kesehatan Vol 7 No 1 (2024)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2024.v7.113-123

Neurodegenerative diseases occur due to dysfunction of the nervous system, which is accompanied by memory and movement disorders. Neurodegenerative diseases can be viewed from an etiological and pathological perspective (pathophysiological and histopathological). The most common neurodegenerative diseases are Huntington's, Alzheimer's, and Parkinson's. This review article will review the etiology and pathology of Huntington's, Alzheimer's, and Parkinson's diseases. The method used in this writing uses journals and books from Google Scholar, PubMed, ResearchGate, and the Web. The keywords used are etiology, pathology, pathophysiology, histopathology, neurodegenerative disease, Huntington's, Alzheimer's, and Parkinson's. The author limited the last 10 years of literature used. Based on a literature review, it is known that the etiology of Huntington's disease is caused by mutations in the huntingtin gene on chromosome four. The pathology of Huntington's disease is caused by unstable expansion of trinucleotide-encoded polyglutamine (CAG) repeats. One of the histopathological features of Huntington's disease can be identified from increased iron levels in the striata of the brain. The etiology of Alzheimer's disease involves interactions between genetic factors, lifestyle, environment, and the aging process. The pathology of Alzheimer's disease occurs due to the presence of apolipoprotein and its relationship to 3 mutated genes. The histopathology of Alzheimer's disease is identified by the presence of neuronal cell death, which is characterized by the shrinking of the nuclei of brain neuronal cells and the cytoplasm has a more eosinophilic color. The etiology of Parkinson's disease is progressive nerve damage to certain areas of the brain. The pathophysiology of Parkinson's disease is thought to involve a reduction in striatal dopamine, which causes an increase in inhibitory output from the globus pallidus pars interna/substantia nigra pars reticulata (Gpi/SNr), resulting in movement suppression. Histopathologically, Parkinson's disease is characterized by degeneration of neurons and neurophagia. This review concludes that Huntington's, Alzheimer's, and Parkinson's diseases can be caused by aging and genetic factors. The pathology of the disease is due to mutations, increased levels of iron, apolipoprotein, neuronal cell death, increased inhibitory output, and neuronal degeneration. The symptoms caused can be motoric, cognitive, and psychiatric.

Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search