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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Media Ilmu Kesehatan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Analis Medika Biosains (JAMBS) JPP Jurnal Kesehatan Poltekkes Palembang Borneo Journal of Medical Laboratory Technology Indonesian Journal of Global Health research Bioscientist : Jurnal Ilmiah Biologi Oryza : Jurnal Pendidikan Biologi Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Prosiding University Research Colloquium JMLS: Journal of Medical Laboratory and Science
Dhanti, Kurnia Ritma
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Borneo Journal of Medical Laboratory Technology

 1 
Optimasi Konsentrasi Primer Dan Suhu Annealing Dalam Mendeteksi Gen Blaz Pada Bakteri Methicillin Resistant Staphylococcus Aureus Di Udara: Optimization of Primary Concentration and Annealing Temperature in Detecting Blaz Gene in Airborne Methicillin-resistant Staphylococcus aureus Bacteria Mardiana, Anisah; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Sulistyowati, Retno
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6076

Abstract

Staphylococcus aureus is a species of bacteria that is often identified as a source of infection and has increased the number of cases of resistance. Resistance to Penicillin-class antibiotics in S. aureus is associated with plasmids containing the blah-Z gene encoding ß-Lactamase. Some factors that can be modified to obtain optimal test results in PCR are annealing temperature and primary concentration. This study aims to identify the primary concentration and annealing temperature that produce PCR products with the best band in detecting the blaZ gene in Metichilin Resistant Staphylococcus aureus (MRSA) bacteria. The research design used used experimental methods. The results of the study obtained were PCR products at primary concentrations of 10 μM were clearly visible, thick and clean, compared to primary concentrations of 5 μM. Annealing temperature with the best band results at temperatures of 61.8 °C and 58.7°C. This study concluded the primary concentration to obtain a clearly visible band result of 10 μM. The annealing temperature of 61.8°C produced the best band in the sample code R.PG. U4, R.V.U2, and R.151.U2 temperatures. While the sample code R.IGD. U5 band results best at annealing temperature 58.7°C.
Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae: Preparation of Coconut Shell Charcoal Gel and Inhibitory Test Against Klebsiella pneumoniae Bacteria Kamalah, Siti Rahmah; Kurniawan, Kurniawan; Mulyanto, Arif; Dhanti, Kurnia Ritma
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6082

Abstract

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.
Optimasi Suhu Annealing Gen Blaz Dari Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Pada Peralatan Medis: Optimization Annealing Temperature Gene blaZ of Bacterial Methicillin-resistant Staphylococcus aureus (MRSA) in Medical Equipment Listiani, Lia; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Widodo, Oei Stefani Yuanita
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6083

Abstract

Methicillin-resistant Staphylococcus aureus is a strain of S. aureus bacteria that is resistant to methicillin-type penicillins. MRSA bacteria can cause nosocomial infections, which can be spread through medical equipment. One of the genes that encodes the resistance trait of S. aureus is the blaZ gene. The detection of the blaZ gene requires optimizing the annealing temperature of the primer used to obtain the optimal PCR band. This study aims to optimize the annealing temperature for the amplification of the blaZ gene DNA from Methicillin-resistant Staphylococcus aureus (MRSA) bacteria on medical devices. The method used in this study used a kit for bacterial extraction and Polymerase Chain Reaction(PCR). The results of the blaZ gene DNA amplification in all samples showed clear bands at temperatures of 52.0°C, 54.2°C, 58.7°C, 61.8°C, and 64.0°C; no smears were formed, and the electrophoretic visualization results on the PCR product showed the results of bands that were according to the target, namely 173 bp. The most optimal annealing temperature for amplification of the blaZ gene in MRSA bacteria from hospital medical equipment is 61.8°C.
Uji Efektifitas Ekstrak Kulit Pisang Kepok (Musa acuminata balbisian colla) dan Daun Salam (Syzygium polyanthum) Sebagai Antibakteri Terhadap Pseudomonas aeruginosa: Test the Effectiveness of Banana Peel Extract Kepok (Musa acuminata balbisian colla) and Bay Leaves (Syzygium polyanthum) as an Antibacterial Pseudomonas aeruginosa Putranti, Respatiningtyas Amadea Danfi; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Mulyanto, Arif
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.8576

Abstract

Pseudomonas aeruginosa merupakan bakteri Gram negatif dari keluarga Pseudomonasadaceae dan terkenal dengan ketahananya yang mampu beradaptasi diberbagai lingkungan, suhu, bersifat aerob dengan mengoksidasi substrat untuk memperoleh energi, dapat bergerak menggunakan flagel dan tahan terhadap berbagai jenis antibiotik. Salah satu tindakan yang dapat dilakukan untuk mengurangi resistensi antibiotik yaitu memanfaatkan tanaman yang berkhasiat sebagai obat. Tujuan dari penelitian ini adalah untuk menguji efektifitas antibakteri ekstrak kulit pisang kepok (Musa acuminata balbisian colla) dan ekstrak daun salam (Syzygium polyanthum) terhadap pertumbuhan bakteri Pseudomonas aeruginosa. Jenis penelitian yang digunakan yaitu true eksperimental post test only with control group design. Metode yang digunakan difusi cakram menggunakan media Nutrient Agar (NA), sampel dibagi menjadi tiga kelompok dengan perbandingan konsentrasi ekstrak kulit pisang kapok dan daun salam (F1 25%:75%) , (F2 50%:50%) , (F3 75%:25%), kontrol positif (kloramfenikol 3µ), kontrol negatif (DMSO 5%), pengulangan dilakukan sebanyak 3 kali. Hasil pengujian yang telah dilakukan menunjukan pembentukan zona hambat pada ketiga perlakuan dengan hasil rata-rata F1 5,2 mm , F2 4,1 mm, F3 3,2 mm. Zona hambat terbesar terbentuk pada K+ , pada kelompok perlakuan zona hambat terbesar yaitu F1 kategori zona hambat lemah sampai sedang yang artinya kelompok perlakuan tidak efektif dalam menghentikan penyebaran bakteri Pseudomonas aeruginosa.
Co-Authors Aida, Seni Nur Aman Suyadi Arief Mulyono Arif Mulyanto, Arif Asmiyenti Djaliasrin Djalil Athala, Syifani Aulia Ahnaf Azizah, Jumrotul Azzahra, Ratu Barkah, Ahmad Cahyaningrum, Winda Rahma Destiawan, Anugroho Farkhan Dita Pratiwi Kusuma Wardani Farida Dwi Handayani Febriani, Siti Amanah Fitriana, Diah Ayu Hanifa, Noha Hayati, Noviani Nur Heny, Dewi Kristiya Nugra Hidayah, Galih Wahyu Junaedi, Arief Juwita, Dina Ratna Kamalah, Siti Rahmah Kurniawan Kurniawan Lestyaninrum, Indah Denissa Listiani, Lia Mardiana, Anisah Mika'il, Hikam Muniroh, Nur An-Nuha Noviana, Ika Putranti, Respatiningtyas Amadea Danfi Putri, Intan Nuraza Rahma, Fitri Iffa Nur Resmi, Herni Nursheta Retno Sulistiyowati RETNO SULISTYOWATI Ristiyanto Ristiyanto Rynando, Nabil Gusapo Santoso, Fasa Bihamdi Lamegri Saputra, Eqwar Sarinastiti, Deliza Dhiakhalda Septiyani, Rani Dwi Sudarsono, Tantri Analisawati Sukowati, Caecilia Hapsari Ceriapuri Sulistio, Ahmad Rizik Sulistiowati, Retno Supriyadi Widodo, Oei Stefani Yuanita Wijayanti, Linda Zahrah, Yasinta Al