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Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae: Preparation of Coconut Shell Charcoal Gel and Inhibitory Test Against Klebsiella pneumoniae Bacteria Kamalah, Siti Rahmah; Kurniawan, Kurniawan; Mulyanto, Arif; Dhanti, Kurnia Ritma
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6082

Abstract

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.
The effectiveness of coconut water in inhibiting shigella sp. bacteria from diarrhea Mujahid, Ikhsan; Mulyanto, Arif; Khasanah, Tyas Ulfah
MEDISAINS Vol 17, No 1 (2019)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v17i1.3796

Abstract

Background: Diarrhea is still a serious problem for some people. It can be affected by the Shigella sp. bacteria. Some villagers drink tender coconut water (Co-cos nucifera L) to cure diarrhea. Some types of consumed tender or green coconut are green coconut, brown coconut, wulung green coconut, and wulung brown coconut. It is necessary to determine the contribution of coconut types to treat diarrhea caused by the Shigella sp bacterium.Objective: to determine the benefit of several types of coconut water toward diarrhea caused by bacteria such as Shigella sp.Method: The method implemented was to determine the benefit of tender coconut water toward Shigella sp bacteria was experimental study through Kirby-Bauer test (antibiotic disc). The study selected water of green coconut, brown coconut, wulung green coconut, and wulung brown coconut, while the positive control used Lodia diarrhea drug and negative control used aquadest (Purified water). Testing data of green coconut water toward Shigella sp. was analyzed through analysis of variance (ANOVA) with F test at 95% significant level. Then it was continued by Duncan test at 95% significant level.Results: The results of testing some green coconut water, positive control and negative control on Shigella sp bacteria can be seen from the clear zone formed around the antibiotic disc, green coconut showed 6.63 mm, brown coconut was 6.57 mm, wulung green coconut was 16.63 mm, wulung brown coconut was 9.23 mm, lodia diarrhea drug was 12.33 mm and purified water was 7.27 mm.Conclusion: all green coconut water is able to inhibit the growth of Shigella sp. The best inhibitor is obtained from the administration of wulung green coconut water with 16.6 mm diameter of the inhibition zone
Uji Efektivitas Antibakteri Ekstrak Etanol Kulit Pisang Kepok (Musa Acuminata Balbisiana Colla) dan Daun Salam (Syzgiyum Polyanthum) Terhadap Bakteri Escherchia Coli Hanifa, Noha; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Mulyanto, Arif
ORYZA ( JURNAL PENDIDIKAN BIOLOGI ) Vol 13 No 2 (2024): ORYZA: Jurnal Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi STKIP Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33627/oz.v13i2.1960

Abstract

This research aims to examine the effectiveness of the combination of bay leaf extract and kepok banana peel extract as an anti Escherichia coli bacteria. This type of research is experimental with a posttest only control group design with a comparison of kepok banana peel and bay leaves, F1 (25%: 75%), F2 (50%: 50%), F3 (75%: 25%). The research results showed that the most effective extract was F1 treatment (25%:75%) with an inhibitory power of 9.7mm2. The results of the statistical analysis showed that the inhibition test treatment against E. Coli bacteria using different concentrations showed a mean difference in the area of ​​the inhibition zone with a significant value of 0.000 (p<0.05). The results of the Duncan Multiple Range Test (DMRT) showed that K+ (chloramphenicol) was significantly different from the concentrations of F1, F2 and F3. Testing the effectiveness of Kepok banana peel extract and bay leaf extract against E. coli bacteria showed that F1 treatment was effective in preventing bacterial growth against the F1 combination of 25% Kepok banana peel and 75% bay leaves.
Hubungan Hygeine Sanitasi Lingkungan dan Tempat Penyimpanan Terhadap Kontaminasi Escherichia coli Pada Bakso di Pasar Purwokerto Putri, Ayuni -; Mulyanto, Arif; Mujahid, Ikhsan; Setiyabudi, Ragil
Journal of Medical Laboratory Research Vol. 2 No. 2 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v2i2.692

Abstract

ABSTRAK Bakso merupakan produk makanan berasal dari olahan daging serta bahan tambahan pangan. Bakso di bedakan menjadi dua jenis yaitu bakso siap saji dan bakso kemasan. Dibeberapa pasar, bakso dalam kemasan diperjualbelikan dengan cara disimpan pada tempat penyimpanan seadanya tanpa memperhatikan hygeine tempat penyimpanan serta hygeine sanitasi lingkungan. Hal ini dapat mempengaruhi kualitas bakso sehingga menimbulkan masalah kesehatan. Salah satu penyebabnya adalah bakteri E.coli disebabkan oleh tempat penyimpanan yang tidak dibersihkan dengan baik serta sanitasi lingkungan buruk. Tujuan dari penelitian ini adalah untuk mengetahui hubungan hygeine sanitasi lingkungan dan tempat penyimpanan terhadap kontaminasi E.coli pada bakso di Pasar Purwokerto. Jenis penelitian ini yaitu observasional deskriptif dengan rancangan pendekatan Cross Sectional. Sampel berupa bakso dalam kemasan sebanyak 12 sampel yang diambil secara random sampling. Hasil penelitian yang telah dilakukan didapatkan kontaminasi bakteri E.coli pada 4 sampel yaitu A2 sebanyak 120 koloni, B2 sebanyak 110 koloni, sebanyak D1 171 koloni dan F1 sebanyak 137 koloni yang ditandai dengan koloni berwarna hijau metalik pada medium EMBA. Menurut uji kolerasi Spermans Rank tidak ada hubungan hygeine sanitasi lingkungan dan tempat penyimpanan terhadap kontaminasi Escherichia coli pada bakso di Pasar Purwokerto, kontaminasi dapat terjadi pada proses pembuatan bakso yang tidak menerapkan hygeine sanitasi penggolahan dengan baik Kata Kunci : Bakso, Escherichia coli, Hygine sanitasi Lingkungan, Hygeine sanitasi Tempat penyimpanan. THE CORRELATION BETWEEN ENVIRONMENTAL HYGIENE STORAGE CONDITIONS AND THE CONTAMINATION OF Escherichia coli IN MEATBALLS SOLD AT PURWOKERTO MARKET Abstract Meatballs, locally known as "bakso", are products made from processed meat and other foodingredients. They can be categorized into two types: ready-to-eat meatballs and packaged meatballs. In severalmarkets, packaged meatballs are sold and stored in arbitrary storage places without considering the storagearea's hygiene or the surrounding environment's sanitation. This lack of care can affect the quality of themeatballs, leading to health issues. One of the main concerns is Escherichia coli contamination, whichcan be attributed to improperly cleaned storage spaces and poor environmental sanitation. Objective: Thisresearch aims to identify the relationship between environmental sanitation hygiene and storage conditionsconcerning the contamination of Escherichia coli in meatballs sold at the Purwokerto Market. Employing a cross-sectional approach, this descriptive observational research randomlysampled twelve packaged meatball samples. Findings: Out of the research, E. coli contamination was found in 4samples. Specifically, Sample A2 had 120 colonies, B2 had 110 colonies, D1 had 171 colonies, and F1 had 137 colonies. Metallic green colonies on the EMBA medium marked this. According to Spearman's Rankcorrelation test, there was no correlation between environmental sanitation hygiene and storage conditionsregarding Escherichia coli contamination in the meatballs at the Purwokerto Market. Contamination might haveoccurred during the meatball production due to inadequate sanitation and hygiene practices. Keywords: Meatballs, Escherichia coli, Environmental Sanitation Hygiene, Hygeine Storage Conditions
PENGARUH VARIASI JUMLAH BLADE TURBIN ANGIN TERHADAP OUTPUT DAYA LISTRIK Wiratama, I Kade; Mara, Made; Mulyanto, Arif; Harianhady, Muliadi
Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin Vol 4, No 1 (2014): Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.88 KB)

Abstract

Some area in Indonesia such as southern part of Sumatra to Nusa Tenggara have big enough wind speed to produce electrical energy by using wind turbine. However, the potential resources are not yet fully developed, and only 1.4 GW electrical energy has been generating in the Indonesia region. Most of the wind energy generating equipment is designed to the European wind speed which is   10 - 30 knot. So, it is very important to modify the such equipment to be able to implement in Indonesia area which is have a lower wind speed.The purpose of this research is to know the influence of number of blades and wind speed to electricity generated by horizontal axis wind turbine with blade taper inversed linear.The result showed that the highest electrical output power was 2,02 watt produced by 5 blades and 3,5 m/s wind speed. The lowest electrical output power was 2,347 watt achieved by using 3 blades and 2,5 m/s wind speed.
Analisis pemakaian bahan bakar biogas termurnikan pada unjuk kerja motor bakar Sutanto, Rudy; Wardani, Kusuma; Mulyanto, Arif; Nurchayati, Nurchayati; Pandiatmi, Pandri; Zainuri, Achmad; Sinarep, Sinarep
Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin Vol 7, No 1 (2017): Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.312 KB) | DOI: 10.29303/dtm.v7i1.1

Abstract

The long term purpose of this research is to obtain the high quality renewable biogas that can be used as fuel for internal combustion engines to drive an electricity generator as energy diversification efforts in the area of small sustainable islands. The specific target expected to be achieved is a method/technique for absorbing impurities contained in biogas, especially CO2 component. The study was conducted to reduce the level of CO2 in biogas using NaOH solution. The mass flow rates of biogas employed were 2, 4, 6, 8 and 10 l/min. The biogas was then tested using an engine to see the effect CO2 on the engine performance at several rotations, i.e. 1500, 2500, 3500, and 4500 rpm. The results show that the torque increases by 21.3% for biogas B2 compared to the unpurified biogas. For biogas B4, the torque increases by 19.1%. While for biogas B6, B8, and B10, the torques increase by 14.9%, 12.8%, and 8.5% respectively. For biogas B2, the SFCE decreases by 33.4%, for biogas B4, the SFCE declines of about 22.7%, while for biogas B6, the SFCE declines by 17.9%. The SFCSs also decline by 13.9% and 8.5% for biogas B8 and B10.
ANALISA PENGARUH LAMA FERMENTASI DAN TEMPERATUR DISTILASI TERHADAP SIFAT FISIK (SPECIFIC GRAVITY DAN NILAI KALOR) BIOETANOL BERBAHAN BAKU NANAS (ANANAS COMOSUS) Sutanto, Rudy; Jaya, Harisman; Mulyanto, Arif
Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin Vol 3, No 2 (2013): Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin
Publisher : Universitas Mataram

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Abstract

 Indonesia is one of the countries which has a largest pineaple plantation in Asia, that is more than 165.690 hektare besides Thailand, Filipina, and Malaysia. Pineaple is one of the plants which contains karbohidrat and sugar as well as corn, cassava, sugar palm, etc. Thus in this occasion the pineaple will be used as material for producing bioetanol cause besides its quantity is large, it’s potential to make bioetanol.            This research head for identify the efect of fermentation periode and distilation  temperature as independent variable to the specific gravity and heating value as dependent variable. In this research the fermentation periode used are 5, 7, and 9 days while distilation temperature are 60, 75, and 800C. Producing of this pineaple bioetanol is doing by fermentation proses. In producing this pineaple bioetanol each sample which mass is 1750 gr added with 75 gr crushed yeast or equal with 4,3 % mass of sample.              From the research it’s found that the alcohol content is increase with fermentation periode and decresing of distilation temperature. Specific gravity decrease with increasing of fermentation periode and decreasing of distilation temperature. Heating value is increase with increasing of fermentation periode and deacreasing of distilation temperature. 
PERBANDINGAN KONDUKTIVITASTEMBAGA, BAJA DAN ALUMINIUM Mulyanto, Arif
Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin Vol 1, No 1 (2011): Dinamika Teknik Mesin: Jurnal Keilmuan dan Terapan Teknik Mesin
Publisher : Universitas Mataram

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Abstract

The heat transfer is important in engineering field and aspects of the life. Many equipment was made by using principles of the heat transfer, like: the oven, iron, the car machine, the muffler, the room cooler et cetera. The principle of the work in the research, there was the heat source at the upper side and the sink (heat remover) at the bottom side so that the temperature different ( was significant) along metal that was tested. The heat source from the electric heater,the object the test togethered with his heater all was isolated. The test object consisted aluminum with 20 mm2 cross section area, the steel and copper have 10 mm2 cross section area. The results that that the conductivity of copper was biggest were followed with aluminum and finally the steel.
Correlation Between HbA1C Levels And C-Reactive Protein Levels In Diabetes Mellitus Patients MUJIONO, TIMBUL; WARDANI, DITA PRATIWI KUSUMA; MULYANTO, ARIF; RAHAJU, MINTO
Jurnal Ilmiah Kesehatan Vol 18 No 01 (2025): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v18i01.5922

Abstract

Diabetes mellitus (DM) is a chronic disease characterised by elevated blood glucose levels ≥ 200 mg/dl due to metabolic disorders. Insulin resistance triggers a blood glucose imbalance, resulting in no energy supply needed by the body's cells. The condition of DM is destructive to the body and, therefore, needs to be measured with stable parameters for the long term. HbA1c is a standard clinical examination used as a determinant of glycaemic status in DM disease. C-reactive protein (CRP) level is an effective parameter in determining the index of damage or inflammation that occurs in the body due to DM. This study aims to determine the relationship between HbA1c levels and CRP levels in patients with diabetes mellitus at Dr. Sardjito Hospital. Twenty patients with type 2 diabetes mellitus were included in this study using the purposive sampling technique. HbA1c data and CRP levels were analysed with the Spearman correlation test. The Pearson correlation test results showed a p-value = 0.001 and an r-value = 0.705. There is a relationship between HbA1c levels and CRP levels in DM patients at RSUD Dr. Soedirman Kebumen.
Comparison of TSH Levels Pulmonary Tuberculosis Patients at The Phase 0 and 6 Months Treatment Qo'imah, Alamanda Nurul; Wardani, Dita Pratiwi Kusuma; Sudarsono, Tantri Analisawati; Mulyanto, Arif
Jurnal Ilmu dan Teknologi Kesehatan Vol 10 No 1 (2022): September 2022
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v10i1.910

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Pulmonary TB has become a health problem worldwide, including in Indonesia, because the sufferers' prevalence is increasing every year. The increase in TB drug resistance will pose a severe health threat. The continuous consumption of drugs in large quantities and high doses can affect the function of the thyroid gland. This study aimed to determine the levels of thyroid stimulating hormone (TSH) in pulmonary tuberculosis patients with treatment phases of 0 months and six months. This research is analytical observational research with a cross-sectional research design. The sampling method uses accidental sampling. A total of 42 respondents participated in this study to determine the levels of TSH in pulmonary TB. The results of the Mann-Whitney U test showed no difference in TSH levels in pulmonary TB patients with different treatment phases of 0 months and six months (p = 0.3). There was no significant difference between TSH levels in pulmonary TB patients in the 0 and 6-month treatment phases.