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Review Journals : Molecular From Anonaceae Syafruddin Ilyas
International Journal of Ecophysiology Vol. 4 No. 1 (2022): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v4i1.11152

There are roughly 120 species of the Anona genus, which is found in tropical and subtropical areas. The diversity at the species level and similarity of the leaves, fruits, and stems that are produced as a result of plant cultivation and hybridization create new plants that are difficult to macroscopically identify. The diversity of the genus Anona can be accurately determined using molecular identification. For example, genetic analysis utilizing PCR and the RAPD and AFLP procedures is one way to gather molecular data. In this study, we inform the development of species-specific primers by differentiating the most agronomically interesting Annona species that, until recently, a new formal classification based on molecular phylogenetics has been developed in order to have a quick and precise method of distinguishing Annona between species agronomically.

Human Papillomavirus: Detection Method and Infection Syafruddin Ilyas
International Journal of Ecophysiology Vol. 4 No. 1 (2022): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v4i1.11153

The Human Papilloma Virus (HPV) is a small non-enveloped DNA virus, around 8000 bp in size and only humans become its host by infecting skin epithelial tissue, human oral mucosa and anogenital epithelium. Human Papillomavirus is often found in patients and is ranked as the second most malignant disease in women, belonging to the Alphapapillomavirus genus. HPV infection can be identified through the structure of the HPV virus itself and the particles contained therein which initiate the carcinogenic process of its host. The research methods used in this study are literature studies. The literature study method is a series of activities related to the method of collecting library data, reading, recording and managing research materials. The method often used to detect HPV is the polymerase chain reaction (PCR). The viral cell reproduction process is aided by activated proteins E1, E2, E4, E5, E6, and E7, which also delay apoptosis and alter the host cell cycle in various ways to cause cell alterations that result in cancer cells.

Does Chitala Lopis Really Extinct? An Overview And DNA Barcode (COI) Comparison Syafruddin Ilyas
International Journal of Ecophysiology Vol. 4 No. 2 (2022): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v4i2.11388

The Asian Featherback Fish Or Ikan Belida Is One Of The Most Important Species Of Fish In Indonesia That Is Utilised By Locals As A Food Ingredient. Unfortunately, One Of The Species (Chitala Lopis) Has Already Been Reported Extinct By The IUCN. Nevertheless, Many Publications After That Still Claimed That Chitala Lopis Is Extant. We Study 28 COI Partial Sequences Of Chitala Lopis From NCBI. The Result Showed That Most Of These Sequences Were Not The Actual Chitala Lopis. It Is Probably Either Chitala Hypselonotus Or Chitala Borneensis. This Prediction Is Based On The Unmatch Between The Distribution Of Chitala Lopis COI Sample Origin And The Actual Habitat Of Chitala Lopis, Which Is Endemic To Java Island. However, There Is Also An Article That Reports If Chitala Lopis Is Tried To Be Cultivated. The Misinterpretation Is Perhaps Because Chitala Spp. Is A Complex Species, Which Has Very Similar Morphology. More Study On Molecular Chitala Sp. In Indonesia Is Still Needed To Complete The Unclear Status Of Extinct Chitala Lopis

Oral Squamous Cell Carcinoma: The Molecular Characteristics Syafruddin Ilyas
International Journal of Ecophysiology Vol. 4 No. 2 (2022): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v4i2.11389

Oral squamous cell carcinoma (OSCC) is a malignancy that occurs on keratinocyte cells that have the potential to spread through lymphatic tissue or blood vessels. These carcinomas develop as a result of a series of molecular factors, which are influenced by individual genetic factors as well as environmental exposure to carcinogens. The evaluation of the prognosis and treatment plan for OSCC are mainly based on the clinical picture of the tumor or the TNM classification (T, tumor; N, lymph nodes or glands; M, metastases). The disadvantage of this diagnostic technique is that it does not consider the biological properties of the tumor, molecular characteristics, or tumor location, so it is deemed incapable of predicting patient safety. The purpose of this paper is to provide an overview of the etiology, pathogenesis, clinical picture, radiographic findings, and histopathological features of OSCC to help those concerned make the correct diagnosis. We use references from the last ten years article to obtain an up-to-date data. Based on our literacy study, we discovered that the presence of pseudoepitheliomatous hyperplasia (PEH) and keratoacanthoma distinguishes OSCC from many other oral cavity carcinomas (CA). The amount of P53 expression in tissues experiencing PEH and CA, as well as the integration of several other pro-cancer protein expressions, can be used to detect severity early. We can conclude that early detection of OSCC should be done by assessing the expression of proteins that cause the severity of cancerous lesions so that suppression of these cancer-inducing proteins can be treated while the levels are still low

Effectiveness of Snakehead Fish Extract on Tooth Extraction Wound Healing HG, Afifah Ayuni; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Archives of The Medicine and Case Reports Vol. 5 No. 1 (2024): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v5i1.479

Tooth extraction can cause open wound in the oral cavity, if not handled properly it can cause complications such as bleeding, infection and delayed wound healing. Wound healing is a biological process associated with tissue growth and regeneration. The wound healing process can be accelerated by administering chemicals that can affect the inflammatory, proliferative and remodeling phases. One of them is by giving snakehead fish extract. The extract contains albumin, copper and zinc which play an important role in the wound healing phase. The purpose of writing this review article is to explain the mechanism of snakehead fish extract in accelerating the healing of tooth extraction wounds. Several studies have revealed that snakehead fish extract affects pro-inflammatory cytokines in helping the wound healing process and significantly helps increase the number of fibroblast cells in tooth socket wounds, so that the healing process can take place more quickly. Based on this, it can be concluded that snakehead fish extract has the potential to accelerate the healing of tooth extraction wounds.

The Potential of Binahong Leaves Extract Nanogel for Alveolar Bone Healing Bernard, Bernard; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Archives of The Medicine and Case Reports Vol. 5 No. 1 (2024): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v5i1.480

Alveolar bone defects pose a significant challenge in dental and oral health, requiring the exploration of novel therapeutic approaches for effective bone healing. The binahong (Anredera cordifolia) leaves are known for their rich bioactive compounds with diverse therapeutic properties, while nanogels, a drug delivery system, offer advantages such as controlled release and enhanced bioavailability. This review explores the potential of binahong leaf extract nanogel as a promising candidate for alveolar bone healing by discussing its preparation, characterization, and properties. Furthermore, it delves into the cellular and molecular mechanisms underlying alveolar bone healing. Based on our review, the effects of binahong leaves extract on bone cells and animal models indicate its therapeutic potential in alveolar bone healing by increasing the expression of growth factors and cells that are essential in bone healing. This review suggests that binahong leaves extract nanogel holds promise as a novel therapeutic intervention for alveolar bone healing. Further research and clinical studies are warranted to fully explore its potential.

Investigation of 3% Binahong (Anredera cordifolia) Leaf Extract Nanogel on the Alveolar Bone Healing: BMP-2 Modulation in Rat Models Bernard, Bernard; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1020

Background: Alveolar bone healing relies on osteoblasts activity and differentiation, with bone morphogenetic protein-2 (BMP-2) playing a crucial regulatory role. Binahong leaf extract (BLE) has demonstrated efficacy in bone healing due to its rich phytochemical composition. Nanogel offers enhanced bioavailability and targeted release to effectively deliver therapeutic agents. This study aims to assess the impact of 3% BLE nanogel on BMP-2 expression in tooth extraction socket healing. Methods: Thirty male Wistar rat underwent anaesthesia for the extraction of their lower incisors to induce alveolar bone healing. Subjects were randomly assigned into treatment (BLE nanogel) and control (base nanogel) group. Five rats from each group were respectively sacrificed at 7, 14, and 28 days after procedures. BMP-2 expression was assessed by performing immunohistochemistry analysis using BMP antibody reagent. Data were analysed using chi-square. Results: The analysis of BMP-2 expression showed no significant difference between treatment and control groups on days 7, 14, and 28 (p>0,05). However, a subtle increase was observed in the treatment group throughout observation period. Conclusion: Application of 3% BLE nanogel may enhance the expression of BMP-2, even though it was not significantly increase. The findings underscore the complex interplay between Binahong leaf extract, nanogel and BMP-2 expression.

Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Riza, Ahyar; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

The Effect of Snakehead Fish (Channa striata) Extract on Dry Socket Wound Healing: TGF-β1 Expression in Rats Model HG, Afifah Ayuni; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1035

Background: Dry socket or alveolar osteitis is a condition characterized by the breakdown of blood clots due to excessive fibrinolysis activity, which disrupts the wound healing process. Consumption of nutrition-rich foods such as snakehead fish (Channa striata) rich in albumin, omega-3 fatty acids, vitamins, minerals, and striatin may accelerate delayed wound healing. This study aimed to analyze the role of snakehead fish extract on the expression of TGF-β1 in the healing of dry socket wounds after tooth extraction in Wistar rats. Methods: This study was an in vivo study with a posttest-only control group design. Forty-five Wistar rats were divided into five groups: snakehead fish extract groups with doses of 1/2, 1, 2, 4 times human dose, and a negative control. The lower left incisor teeth of the rats were extracted, then dry socket was induced with adrenaline using a paper point. On days 3, 7, and 14, TGF-β1 expression was assessed using immunohistochemical staining. Results: This study showed significant difference in TGF-β1 expression on days 3, 7, and 14. TGF-β1 expression was higher in the treatment groups than the control (p<0.05). Conclusion: Administration of snakehead fish extract increased TGF-β1 expression. These findings underline the complex interaction between snakehead fish extract and TGF-β1 expression.

Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Riza, Ahyar; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

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