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All Journal Jurnal Kesehatan Masyarakat Indonesia E-Jurnal Medika Udayana Jurnal Kesehatan Lingkungan indonesia E-Dimas: Jurnal Pengabdian kepada Masyarakat Acta Biochimica Indonesiana PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Abdi Insani Science Midwifery Community Development Journal: Jurnal Pengabdian Masyarakat Mattawang: Jurnal Pengabdian Masyarakat Narra J AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Warmadewa Minesterium Medical Journal INCOME: Indonesian Journal of Community Service and Engagement Community Services Journal (CSJ) Jurnal Widya Medika
Lestarini, Asri
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Development of decellularized mouse auricular scaffolds using sodium dodecyl sulfate immersion-agitation for microtia tissue engineering Jaya, Putu KD.; Dewi, Anak AAAP.; Lestarini, Asri; Witari, Ni PD.; Evayanti, Luh G.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.1610

Abstract

Effective treatment strategies for microtia remain limited due to the side effects and shortcomings associated with current therapeutic approaches. Tissue engineering, particularly the development of biological scaffolds, has emerged as a promising alternative. However, research on auricular scaffold fabrication in murine models using sodium dodecyl sulfate (SDS) and the immersion–agitation decellularization technique remains scarce. The aim of this study was to evaluate the effects of varying SDS concentrations on the decellularization efficiency and extracellular matrix (ECM) preservation of murine auricular tissue for scaffold development. Auricular tissues from mice (n=4) were immersed in Erlenmeyer flasks containing 0.1%, 0.5%, or 1% SDS and subjected to continuous agitation until the tissues became macroscopically translucent. Qualitative assessments included macroscopic appearance and microscopic evaluation using hematoxylin–eosin and Masson's trichrome staining. Quantitative analysis involved counting residual nuclei, while semiquantitative analysis of ECM area fractions was performed using ImageJ software. Statistical comparisons were conducted using one-way analysis of variance (ANOVA), with significance defined as p<0.05. The results demonstrated that the decellularized scaffolds exhibited macroscopic translucency, significantly reduced nuclear content (p=0.001), and preserved ECM integrity (p=0.012). Among the tested concentrations, 0.5% SDS provided the optimal balance between effective decellularization and ECM preservation. These findings support the potential application of murine auricular scaffolds decellularized with 0.5% SDS via the immersion–agitation method for future microtia tissue engineering.
Co-Authors Anak Agung Gede Raka Gunawarman Anak Agung Sri Agung Aryastuti Aryastuti , Anak Agung Sri Agung Cahyawati , Putu Nita Cahyawati, Putu Nita Danti, Ida Ayu Mahari Datya , Aulia Iefan Dewi, AA Ayu Asri Prima Dewi, Anak AAAP. Dewi, Anak Agung Istri Agung Putri Septiani DEWI, SARASWATI LAKSMI Dwiyami, Made Indriyani Evayanti, Luh G. I Gde Suranaya Pandit I Nengah Muliarta, I Nengah I Wayan Sudiarta Jaya, I Gede Putra Angga Jaya, Putu KD. Masyeni, Sri Ni Luh Putu Putri, Setianingsih Ni Made Rustini, Ni Made Ningsih, Ni Luh Anik Puspa Pande Ayu Naya Kasih Permatananda Paramitha, I Gusti Ayu Mas Diah Prabandewi, Ni Luh Putu Ratih Purnomo, Amelia Putri Putra, I Putu Rustama Putri, Anak Agung Ayu Radismarani Riandra, Ni Putu Indah Kusumadewi Ruspita Sari, Ni Ketut Adelia Sari, Ni Luh Putu Eka Kartika Sintya, Erly Sumadewi, Komang Trisna Tanjung Subrata Tiba, Andika Umbu Udiyani , Desak Putu Citra Witari, Ni PD. Yogiswara, Gde Candra Yoni, Putu Diah Paramitha Dwi