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Struktur Komunitas Collembola Pada Habitat Gua Lowo dan Kebun Warga di Desa Melirang Kabupaten Gresik, Jawa Timur Ria Safitri; Siti Zulaikha; Saiful Bahri; Saiku Rokhim; Ita Ainun Jariyah; Nirmala Fitria Firdhausi
Jurnal Pro-Life Vol. 9 No. 3 (2022): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Collembola is one of the soil fauna that acts as the smallest constituent component for the balance of the ecosystem. This study aims to compare the structure of the Collembola community in the habitat of Lowo Cave and Citizen's Garden in Melirang Village, Gresik Regency. This research uses descriptive exploratory research with soil drill method for soil sampling and pitfall trap for ground surface insect traps. Based on Collembola data obtained in Lowo Cave, there are 3 species with a total of 87 individuals, while in Citizen's Garden there are 6 Collembola species with a total of 145 individuals The results of the analysis of this study indicate that the Diversity Index value in the Lowo Cave habitat is H'= 0.859735 and in the Citizen's Garden a habitat with the value H' = 1.475675, the Evenness Index value in the Lowo Cave is E = 0.782565 and in the Citizen's Garden with a value of E = 0.82359, the value of the Dominance Index in Gua Lowo is 0.48104 and in the Citizen's Garden is 0.282949, the Relative Frequency of Ascocyrtus sp. and Hypogastrura consanguinea have a relative frequency of 100%.

Diversity and Community Structure of Dragonflies (Odonata) in Various Types of Habitat at Lakarsantri District, Surabaya, Indonesia Muhamad Azmi Dwi Susanto; Nirmala Fitria Firdhausi; Saiful Bahri
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76690

Dragonflies are insects that are very dependent on the existence of freshwater ecosystems, However, the population of dragonflies in urban freshwater ecosystems is at risk due to a number of issues. Consequently, it is essential to carry out research and efforts to preserve dragonflies in urban areas. This study aims to provide information about the diversity and structure of dragonfly communities in various habitat types in the Lakarsantri, Surabaya. Data collection in this study was carried out in the habitat types of ponds, reservoir, river, and rice field in July to September 2021. The study results show there are 22 species from 4 families with a total of 827 individuals. Analysis of the Shannon-Wiener diversity index showed that the highest value of dragonfly diversity was found in a pond, with a value of H' = 2.40, and the location with the lowest value was a river, with a value of H' = 1.77. At four research locations that have different aquatic ecosystems, the community structure of dragonfly is also different. The composition of the dragonfly community structure at the reservoir location has similarities to a pond, and at a river location, it has similarities to a paddy field. Differences in abiotic factors consisting of light intensity, humidity, and temperature at each study location have a correlation with differences in dragonfly community structure. In addition, the composition of the vegetation at each location is also one of the factors causing differences in the structure of the dragonfly community.

PENGARUH KONSENTRASI DAN LAMA PERENDAMAN KOLKISIN TERHADAP KARAKTER POLIPLOIDI PADA TANAMAN LILI HUJAN (Zephyranthes rosea Lindl.) Putri, Vivin Diana; Faizah, Hanik; Firdhausi, Nirmala Fitria
Jurnal Pro-Life Vol. 10 No. 3 (2023): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Zephyranthes rosea Lindl. is one of Indonesia's export commodities. Research on the induction of polyploid Z. rosea to increase sales value by increasing its phenotype is still limited and limited to seed organs. The aim of this research was to determine the effect of colchicine concentration, soaking time, and the interaction of the two on polyploidy characters. Z. rosea root organs were treated with colchicine (0%, 0.025%, 0.05%, 0.075%, and 0.1%) and different soaking time (6 hours, 12 hours, 18 hours, and 24 hours) in this study. The result showed that there was a significant effect of colchicine concentration, soaking time, and the interaction of of the two on leaf length, stomata density, and dry weight while flowering time, leaf width, stomata length, stomata width, fresh weight, and flower color were not significantly affected. The lowest leaf length was produced by 0.1% colchicine (21.392 cm), 6 hours of soaking time (22.693 cm), and the interaction of 0.1% colchicine with 6 hours of soaking time (20.633 cm). The highest density of stomata resulted from 0.025% colchicine (787.247/mm2), 6 hours of soaking time (762.626/mm2), and the interaction of 0.025% colchicine with 24 hours of soaking time (906.566/mm2). The highest dry weight resulted from colchicine 0.075% (16.433 gram), soaking time of 12 hours (16.527 gram), and interaction of colchicine 0.075% with soaking time of 12 hours (19.467 gram). Because there were more polyploid characters which were not significantly affected, the polyploid Z. rosea had not been formed in this study.

EFEKTIVAS BEAUVERIA BASSIANA TERHADAP PERTUMBUHAN TANAMAN TOMAT (Solanum lycopersicum L.) YANG TERINFEKSI CENDAWAN Fusarium oxysporum: EFFECTIVENESS OF BEAUVERIA BASSIANA ON THE GROWTH OF TOMATO PLANTS (Solanum Lycopersicum L.) INFECTED WITH THE FUNGUS Fusarium Oxysporum Khodijah, Siti; Firdhausi, Nirmala Fitria; Faizah, Hanik
AgriPeat Vol. 25 No. 02 (2024): JURNAL AGRIPEAT VULUME 25 NOMOR 02 SEPTEMBER 2024
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v25i02.15185

Tanaman tomat (Solanum lycopersicum L.) banyak dibudidayakan hampir diseluruh dunia. Tiap tahun tanaman tomat memiliki penurunan produksi yang diakibatkan oleh banyak hal, salah satunya adalah serangan Fusarium oxysporum. Salah satu cara untuk mengendalikan penyakit F. oxysporum adalah dengan penggunaan agen pengendali hayati, yaitu Beauveria bassiana. Selain dapat meningkatkan ketahanan tanaman dari serangan organisme pengganggu, B. bassiana juga memacu pertumbuhan dan perkembangan tanaman. Penelitian ini bertujuan untuk menentukan waktu pemberian B. bassiana yang efektif dalam mengatasi serangan layu tanaman tomat. Desain penelitian ini menggunakan RAL. Pengujian B. bassiana dilakukan pada 3 dan 7 hari sebelum tanam, 3 dan 7 hari sesudah tanam, dan saat tanam. Data diuji menggunakan ANOVA dan Duncan 5%. Hasil menunjukkan B. bassiana telah memenuhi standar mutu sebagai agen pengendali hayati sesuai Keputusan Menteri Pertanian No. 70/Permentan/SR.140/19/2011 serta telah sesuai SNI 8027.1: 2014. Seluruh perlakuan pemberian B. bassiana mampu menekan kelayuan patogen hingga 0,4 %, B. bassiana juga berpengaruh pada tinggi tanaman, jumlah cabang, jumlah daun, panjang akar, panjang daun, bobot kering dan basah tanaman. Pada penelitian ini perlakuan B. bassiana terbaik untuk pertumbuhan dan hasil adalah T4, yaitu pemberian B. bassiana 3 hari setelah tanam.

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata) Choiriyah, S.Si, Chumaidatul; Firdhausi, Nirmala Fitria; Tyastirin, Esti; Rachmawati, Yuanita; Hadi, Moch. Irfan
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p80-87

Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.

PEMANTAUAN KUALITAS MIKROBIOLOGIS PADA PERMUKAAN MEJA KERJA PENGUJIAN LABORATORIUM MIKROBIOLOGI SEBELUM DI DESINFEKSI BERDASARKAN TOTAL PLATE COUNT DENGAN METODE SWAB Farah Syifa Zayyanna, Salma; Fitria Firdhausi, Nirmala
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5635

Microbiology laboratory environmental monitoring is an activity that must be considered to ensure that there is no contamination either directly or cross-contamination. Environmental monitoring can be carried out using the swab method in accordance with SNI 2332.3: 2015. This study shows that the sample preparation room has a high contamination rate exceeding the limits specified in SNI 2332.3: 2015. Contamination in the sample preparation room has a figure of 1.4 x 104 cfu / 100 cm2. Inoculation room 2 (LAF) has the lowest contamination rate, this is influenced by the pressure around the room which is useful for reducing contamination. This monitoring must be carried out periodically to ensure that the environmental conditions are clean and sterile, thereby reducing the possibility of contamination of products and test results.

PEMANTAUAN KUALITAS MIKROBIOLOGIS PADA PERMUKAAN MEJA KERJA PENGUJIAN LABORATORIUM MIKROBIOLOGI SEBELUM DI DESINFEKSI BERDASARKAN TOTAL PLATE COUNT DENGAN METODE SWAB Farah Syifa Zayyanna, Salma; Fitria Firdhausi, Nirmala
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5635

Microbiology laboratory environmental monitoring is an activity that must be considered to ensure that there is no contamination either directly or cross-contamination. Environmental monitoring can be carried out using the swab method in accordance with SNI 2332.3: 2015. This study shows that the sample preparation room has a high contamination rate exceeding the limits specified in SNI 2332.3: 2015. Contamination in the sample preparation room has a figure of 1.4 x 104 cfu / 100 cm2. Inoculation room 2 (LAF) has the lowest contamination rate, this is influenced by the pressure around the room which is useful for reducing contamination. This monitoring must be carried out periodically to ensure that the environmental conditions are clean and sterile, thereby reducing the possibility of contamination of products and test results.

Phytochemical Screening and Antimicrobial Activity of Combination of Piper betle and Moringa oleifera Extracts Arifin, Siti Hakimah Aprilia Garini; Firdhausi, Nirmala Fitria; Hidayati, Irul; Faizah, Hanik
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 2 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i2.45288

The inappropriate use of antibiotics can lead to pathogenic bacteria developing multiple drug resistance, making infectious disease treatment crucial. Thus, the discovery of novel antibiotics from natural materials, such as plant extracts or their combinations, is critically needed. Extracts of green betel (Piper betle) and Moringa (Moringa oleifera) leaves are known to have antimicrobial activity. This study aimed to determine the chemical content and antimicrobial activity of ethanol extracts of the combination of green betel leaves and Moringa leaves against Staphylococcus aureus, Escherichia coli, and Candida albicans. Green betel and Moringa leaves were macerated in 96% ethanol to extract secondary metabolites. Secondary metabolites were analyzed using phytochemical screening. Antimicrobial activity was tested using disk diffusion using various concentrations: EF1 (25% betel leaves + 75% moringa leaves), EF2 (50% betel leaves + 50% moringa leaves), and EF3 (75% betel leaves + 25% moringa leaves). Phytochemical tests showed that the ethanol extracts of betel leaves contained flavonoids, alkaloids, tannins, and saponins, and no terpenoids, while moringa leaves contain flavonoids, tannins, saponins, and terpenoids, but no alkaloids. S. aureus and E. coli had the highest inhibition zones at EF3 (75% betel leaves + 25% moringa leaves) concentrations, with average inhibition zones of 21.72 mm and 20.98 mm, respectively, and C. albicans at EF2 (50% betel leaves + 50% moringa leaves) concentration, with an average inhibition zone of 26.52 mm, with a very strong inhibition category.

Pengaruh Penambahan Kulit Jeruk Manis Dalam Berbagai Konsentrasi Dan Lama Fermentasi Terhadap Sifat Fisik Dan Kimia Nata De Citrus Dari Sari Buah Jeruk Manis (Citrus Sinensis) Matlubah, sheva; Nirmala Fitria Firdhausi; Hanik Faizah
Jurnal Agritechno Jurnal Agritechno Vol. 18, Nomor 2, Oktober 2025
Publisher : Depertemen Teknologi Pertanian Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar

Nata is a gel-shaped polysaccharide fermented by Acetobacter xylinum bacteria, with nata de coco as the most well-known product. The naming of nata is now adjusted to the media or fruit juice used, but the use of sweet orange juice and peel is still rarely done. This study aims to determine the effect of sweet orange peel concentration and fermentation time on the characteristics of nata from sweet orange juice. The method used is a Completely Randomized Design (CRD) and analyzed by ANOVA test. Treatment variations include sweet orange peel concentration (0.5%, 1%, and 1.5%) and fermentation time (10, 14, and 18 days). The parameters observed include physical and chemical properties. The results showed that the physical characteristics, namely the highest thickness and yield in sample E (1% sweet orange peel, 14 days fermentation) with values of 2.17 mm and 18.72%, respectively. Meanwhile, the highest water content was found in sample G (0.5% sweet orange peel, 18 days fermentation) at 76.8%, and the highest cellulose content was recorded in sample J (positive control) at 3.066%.

Potensi Tumbuhan Melati Air (Echinodorus radicans) Terhadap Penurunan Kadar Timbal (Pb) dan Tembaga (Cu) Pada Limbah Cair Home Industry Batik di Desa Sendang Kabupaten Lamongan Fatiha, Irssa Intan; Firdhausi, Nirmala Fitria; Zummah, Atiqoh
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14892

This study aims to determine the potential of water jasmine plants for optimal absorption time, reduction of heavy metal levels of Pb and Cu in Sendang batik liquid waste, and to observe plant morphology. This study uses an experimental method with a one-factor Completely Randomized Design (CRD) and four repetitions, resulting in 16 experimental plots. Heavy metal testing uses an Atomic Absorption Spectrophotometer (AAS). The results showed that (1) on days 9, 12, 15, and 18 of exposure, the decrease in Pb heavy metal levels was 3.71, 2.65, 0.80, and 0. 14 ppm, heavy metal Cu levels decreased consecutively by 4.09, 2.85, 0.31 and 0.26 ppm. (2) The average absorption rate of heavy metal Pb at exposure times of 9, 12, 15, and 18 consecutive days was 0.8, 0.14, 0.24 and 0.23 ppm, respectively, and the heavy metal Cu was 0.23, 0.28, 0.40 and 0. 33 ppm. (3) Morphological changes, namely the appearance of 2-3 new shoots for each treatment, the stems drooping slightly, the leaves changing color to yellow to brownish and brown spots throughout the treatment and on the 18th day one of the leaves died. (4) The initial results of the statistical test for Pb and Cu metals have a sig value of >0.05, where the data is normally distributed and homogeneous. (5) In the One Way Anova test results, sig = 0.000Ë‚0.05 or there is an effect of exposure time and the post hoc Pb test results are Ë‚0.05 while some Cu metals are Ëƒ0.05.

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