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Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata) Choiriyah, S.Si, Chumaidatul; Firdhausi, Nirmala Fitria; Tyastirin, Esti; Rachmawati, Yuanita; Hadi, Moch. Irfan
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p80-87

Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.

PEMANTAUAN KUALITAS MIKROBIOLOGIS PADA PERMUKAAN MEJA KERJA PENGUJIAN LABORATORIUM MIKROBIOLOGI SEBELUM DI DESINFEKSI BERDASARKAN TOTAL PLATE COUNT DENGAN METODE SWAB Farah Syifa Zayyanna, Salma; Fitria Firdhausi, Nirmala
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5635

Microbiology laboratory environmental monitoring is an activity that must be considered to ensure that there is no contamination either directly or cross-contamination. Environmental monitoring can be carried out using the swab method in accordance with SNI 2332.3: 2015. This study shows that the sample preparation room has a high contamination rate exceeding the limits specified in SNI 2332.3: 2015. Contamination in the sample preparation room has a figure of 1.4 x 104 cfu / 100 cm2. Inoculation room 2 (LAF) has the lowest contamination rate, this is influenced by the pressure around the room which is useful for reducing contamination. This monitoring must be carried out periodically to ensure that the environmental conditions are clean and sterile, thereby reducing the possibility of contamination of products and test results.

PEMANTAUAN KUALITAS MIKROBIOLOGIS PADA PERMUKAAN MEJA KERJA PENGUJIAN LABORATORIUM MIKROBIOLOGI SEBELUM DI DESINFEKSI BERDASARKAN TOTAL PLATE COUNT DENGAN METODE SWAB Farah Syifa Zayyanna, Salma; Fitria Firdhausi, Nirmala
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5635

Microbiology laboratory environmental monitoring is an activity that must be considered to ensure that there is no contamination either directly or cross-contamination. Environmental monitoring can be carried out using the swab method in accordance with SNI 2332.3: 2015. This study shows that the sample preparation room has a high contamination rate exceeding the limits specified in SNI 2332.3: 2015. Contamination in the sample preparation room has a figure of 1.4 x 104 cfu / 100 cm2. Inoculation room 2 (LAF) has the lowest contamination rate, this is influenced by the pressure around the room which is useful for reducing contamination. This monitoring must be carried out periodically to ensure that the environmental conditions are clean and sterile, thereby reducing the possibility of contamination of products and test results.

Phytochemical Screening and Antimicrobial Activity of Combination of Piper betle and Moringa oleifera Extracts Arifin, Siti Hakimah Aprilia Garini; Firdhausi, Nirmala Fitria; Hidayati, Irul; Faizah, Hanik
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 2 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i2.45288

The inappropriate use of antibiotics can lead to pathogenic bacteria developing multiple drug resistance, making infectious disease treatment crucial. Thus, the discovery of novel antibiotics from natural materials, such as plant extracts or their combinations, is critically needed. Extracts of green betel (Piper betle) and Moringa (Moringa oleifera) leaves are known to have antimicrobial activity. This study aimed to determine the chemical content and antimicrobial activity of ethanol extracts of the combination of green betel leaves and Moringa leaves against Staphylococcus aureus, Escherichia coli, and Candida albicans. Green betel and Moringa leaves were macerated in 96% ethanol to extract secondary metabolites. Secondary metabolites were analyzed using phytochemical screening. Antimicrobial activity was tested using disk diffusion using various concentrations: EF1 (25% betel leaves + 75% moringa leaves), EF2 (50% betel leaves + 50% moringa leaves), and EF3 (75% betel leaves + 25% moringa leaves). Phytochemical tests showed that the ethanol extracts of betel leaves contained flavonoids, alkaloids, tannins, and saponins, and no terpenoids, while moringa leaves contain flavonoids, tannins, saponins, and terpenoids, but no alkaloids. S. aureus and E. coli had the highest inhibition zones at EF3 (75% betel leaves + 25% moringa leaves) concentrations, with average inhibition zones of 21.72 mm and 20.98 mm, respectively, and C. albicans at EF2 (50% betel leaves + 50% moringa leaves) concentration, with an average inhibition zone of 26.52 mm, with a very strong inhibition category.

Pengaruh Penambahan Kulit Jeruk Manis Dalam Berbagai Konsentrasi Dan Lama Fermentasi Terhadap Sifat Fisik Dan Kimia Nata De Citrus Dari Sari Buah Jeruk Manis (Citrus Sinensis) Matlubah, sheva; Nirmala Fitria Firdhausi; Hanik Faizah
Jurnal Agritechno Jurnal Agritechno Vol. 18, Nomor 2, Oktober 2025
Publisher : Depertemen Teknologi Pertanian Universitas Hasanuddin

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Nata is a gel-shaped polysaccharide fermented by Acetobacter xylinum bacteria, with nata de coco as the most well-known product. The naming of nata is now adjusted to the media or fruit juice used, but the use of sweet orange juice and peel is still rarely done. This study aims to determine the effect of sweet orange peel concentration and fermentation time on the characteristics of nata from sweet orange juice. The method used is a Completely Randomized Design (CRD) and analyzed by ANOVA test. Treatment variations include sweet orange peel concentration (0.5%, 1%, and 1.5%) and fermentation time (10, 14, and 18 days). The parameters observed include physical and chemical properties. The results showed that the physical characteristics, namely the highest thickness and yield in sample E (1% sweet orange peel, 14 days fermentation) with values of 2.17 mm and 18.72%, respectively. Meanwhile, the highest water content was found in sample G (0.5% sweet orange peel, 18 days fermentation) at 76.8%, and the highest cellulose content was recorded in sample J (positive control) at 3.066%.

Potensi Tumbuhan Melati Air (Echinodorus radicans) Terhadap Penurunan Kadar Timbal (Pb) dan Tembaga (Cu) Pada Limbah Cair Home Industry Batik di Desa Sendang Kabupaten Lamongan Fatiha, Irssa Intan; Firdhausi, Nirmala Fitria; Zummah, Atiqoh
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14892

This study aims to determine the potential of water jasmine plants for optimal absorption time, reduction of heavy metal levels of Pb and Cu in Sendang batik liquid waste, and to observe plant morphology. This study uses an experimental method with a one-factor Completely Randomized Design (CRD) and four repetitions, resulting in 16 experimental plots. Heavy metal testing uses an Atomic Absorption Spectrophotometer (AAS). The results showed that (1) on days 9, 12, 15, and 18 of exposure, the decrease in Pb heavy metal levels was 3.71, 2.65, 0.80, and 0. 14 ppm, heavy metal Cu levels decreased consecutively by 4.09, 2.85, 0.31 and 0.26 ppm. (2) The average absorption rate of heavy metal Pb at exposure times of 9, 12, 15, and 18 consecutive days was 0.8, 0.14, 0.24 and 0.23 ppm, respectively, and the heavy metal Cu was 0.23, 0.28, 0.40 and 0. 33 ppm. (3) Morphological changes, namely the appearance of 2-3 new shoots for each treatment, the stems drooping slightly, the leaves changing color to yellow to brownish and brown spots throughout the treatment and on the 18th day one of the leaves died. (4) The initial results of the statistical test for Pb and Cu metals have a sig value of >0.05, where the data is normally distributed and homogeneous. (5) In the One Way Anova test results, sig = 0.000Ë‚0.05 or there is an effect of exposure time and the post hoc Pb test results are Ë‚0.05 while some Cu metals are Ëƒ0.05.

Composition and Zonation of Mangrove Vegetation in Ujung Pangkah, Gresik, Indonesia Mabruroh, Arofatul Syabina; Firdhausi, Nirmala Fitria; Bahri, Saiful; Rachmawati, Yuanita; Munir, Misbakhul
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 2: September 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i2.23819

Ujung Pangkah Gresik is a significant mangrove area with both ecological and economic functions. Ecologically, it prevents seawater intrusion and abrasion, while economically, it supports sectors like tourism, forestry, fisheries, and ecotourism. This research aims to identify mangrove species diversity and zoning patterns in Ujung Pangkah using survey methods to assess diversity, density, dominance, importance value, and zoning. Four species were found (Avicennia marina, Avicennia officinalis, Rhizophora stylosa, Ceriops tagal) with 454 individuals. The diversity value is moderate at 1.86. The highest and lowest densities were 0.52 and 0.01, respectively. Avicennia marina is the dominant species with an importance index of 273.69. The area has a mixed zoning pattern.

Analisis Keanekaragaman dan Inventarisasi Jenis Burung di Kawasan Wisata Coban Talun, Kota Batu, Jawa Timur Febiyanti, Salma Fakhirah; Wati, Diana Rahma; Wibisana, Oki Rahmatirta; Firdausi, Nirmala Fitria
Jurnal Hutan Tropis Vol 13, No 4 (2025): Jurnal Hutan Tropis Volume 13 Nomer 4 Edisi Desember 2025
Publisher : Lambung Mangkurat University-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jht.v13i4.24105

Penelitian ini bertujuan untuk melakukan inventarisasi jenis burung di kawasan wisata Coban Talun, Kota Batu, Jawa Timur. Burung berperan penting dalam ekosistem sebagai penyebar biji, penyerbuk, dan pengendali serangga sehingga keberadaannya menjadi indikator kualitas lingkungan. Metode pengamatan yang digunakan adalah point count dan line transect pada dua stasiun, yaitu hutan pinus dan ladang terbuka, yang dilakukan pada pagi hari. Data yang dikumpulkan mencakup jenis, famili, nama lokal, serta jumlah individu burung yang teramati, kemudian dianalisis menggunakan indeks keanekaragaman Shannon-Wiener. Hasil penelitian menunjukkan terdapat 24 spesies burung dari 16 famili. Stasiun hutan pinus mencatat 16 spesies dari 13 famili dengan spesies dominan Cucak Kutilang (Pycnonotus aurigaster) dan nilai indeks keanekaragaman sebesar 2,40 (kategori sedang). Stasiun ladang mencatat 15 spesies dari 10 famili dengan spesies dominan Walet Linci (Collocalia linchi) dan nilai indeks keanekaragaman sebesar 2,37 (kategori sedang). Perbedaan komposisi spesies dan nilai indeks yang hampir sama menunjukkan bahwa kondisi vegetasi pada masing-masing habitat berpengaruh terhadap keberadaan burung, di mana hutan pinus dengan tegakan rapat memberikan keragaman yang lebih merata sedangkan ladang terbuka lebih dipengaruhi oleh dominansi spesies tertentu. Hasil penelitian ini dapat menjadi dasar dalam upaya konservasi serta pengelolaan kawasan wisata Coban Talun secara berkelanjutan.

UJI KUALITAS MIKROBIOLOGI PADA DAGING AYAM BROILER YANG DIJUAL DI PASAR TRADISIONAL KECAMATAN BANGIL Muhtar, Nurul Maulidiya; Faizah, Hanik; Firdhausi, Nirmala Fitria
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 2 (2025): BIOTIKA DESEMBER 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i2.64903

Keamanan pangan merupakan aspek penting yang menentukan kualitas kesehatan masyarakat, terutama pada produk hewani seperti daging ayam. Penanganan dan penyimpanan yang tidak higienis dapat menyebabkan terjadinya kontaminasi mikroba yang berpotensi menurunkan kualitas serta membahayakan konsumen. Penelitian ini bertujuan untuk mengetahui kualitas mikrobiologi daging ayam broiler yang dijual di pasar tradisional Kecamatan Bangil, Kabupaten Pasuruan, dengan fokus pada parameter Total Plate Count (TPC), keberadaan Escherichia coli, dan Salmonella sp. Metode yang digunakan adalah deskriptif kualitatif dengan teknik sampling jenuh. Sebanyak 10 sampel daging diuji menggunakan metode TPC, media EMBA untuk E. coli, dan media SSA untuk Salmonella sp. Hasil uji menunjukkan bahwa satu sampel (10%) melebihi ambang batas TPC berdasarkan SNI 7388:2009 yaitu 1×10⁶ CFU/g, sedangkan sembilan lainnya masih dalam batas aman. Pengujian E. coli menunjukkan bahwa 3 dari 10 sampel (30%) positif terkontaminasi, dan uji Salmonella sp. juga menunjukkan 3 sampel (30%) positif. Temuan ini menunjukkan bahwa meskipun sebagian besar sampel memenuhi standar mutu mikrobiologis, masih terdapat risiko kontaminasi patogen yang dapat membahayakan kesehatan konsumen jika daging tidak dimasak dengan benar. Oleh karena itu, diperlukan pengawasan sanitasi dan edukasi terkait penanganan daging ayam di pasar tradisional

Keanekaragaman Tumbuhan Berkhasiat Obat Pada Suku Noaulu Di Pulau Seram, Maluku Sarmawaty Kotala; Nirmala Fitria Firdhausi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.1.49-55

Noaulu an indigenous tribe of Seram Island who live in the Sepa Village of Amahai District, Maluku. This tribe still use forest products to fulfill their needs, including medication. Therefore, this study intends to know the kinds of medicinal plants used by the Noaulu Tribe and the types of diseases that can be treated. Collecting data in this study using survey method and an interview method with the public interest. Determination of the respondents was using purposive sampling method. Inventory of medicinal plants was done by a survey method survey based respondent information. The result of the research shows that there are 31 medicinal plants from 22 families. They are able to cure 20 diseases. The plants which are predominantly used are from Zingiberaceae family. Leaves of the plant are mostly used as the drugs.

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