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Harvianti, Yuniar
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Lansium domesticum metabolites interfere with digestive enzyme function in the rice weevil sitophilus oryzae Oktariansyah, Yadi; Putri, Ferta Liza; Harvianti, Yuniar; Musifa, Eva
JPBIO (Jurnal Pendidikan Biologi) Vol 11, No 1 (2026): April 2026
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v11i1.6212

Abstract

Pectin methylesterase (PME) plays a key role in polysaccharide digestion in the rice weevil Sitophilus oryzae, making it a potential molecular target for pest control. This study employed structure-based in silico analysis to evaluate selected metabolites of Lansium domesticum as PME inhibitors. Representative compounds were chosen according to predicted interference mechanisms and docked into the validated catalytic site. All complexes showed structural stability, while interaction patterns revealed six inhibition modes: competitive mimicry, catalytic disruption, orientation interference, channel blocking, polymer mimicry, and interfacial modulation, whereas polyphenols and glycosides produced the strongest interference. The findings indicate that L. domesticum metabolites can disrupt digestive enzymatic processes through multiple complementary pathways, supporting their potential development as biologically derived pest management agents.
Aktivitas Antagonistik Bakteri PGPR Citrobacter freundii dan Pseudomonas aeruginosa terhadap Patogen Tular Tanah Padi Harvianti, Yuniar; Kasiamdari, Rina Sri
Filogeni: Jurnal Mahasiswa Biologi Vol 6 No 1 (2026): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v6i1.60141

Abstract

Hawar pelepah salah satu penyakit penting tanaman padi yang disebabkan oleh cendawan patogen tular tanah yang mampu menyebabkan kehilangan hasil panen hingga 50%. Salah satu pendekatan pengendalian yang ramah lingkungan dan berkelanjutan adalah melalui pemanfaatan bakteri rhizosfer sebagai agen biokontrol, khususnya kelompok Plant Growth Promoting Rhizobacteria (PGPR). Penelitian ini bertujuan untuk mengevaluasi potensi dua spesies bakteri, Citrobacter freundii LMG 3246 dari sawah organik dan Pseudomonas aeruginosa dari sawah nonorganik tanaman padi kultivar Ciherang di Provinsi Sulawesi Selatan dalam menghambat pertumbuhan patogen hawar pelepah padi secara in vitro. Penelitian ini dilakukan dengan isolasi dan identifikasi molekuler cendawan patogen penyebab hawar pelepah padi kemudian menguji potensi bakteri rhizosfer melalui penghambatan hifa dan perkecambahan sklerotia cendawan patogen. Hasil penelitian menunjukkan bahwa cendawan patogen penyebab hawar pelepah padi memiliki kesamaan dengan spesies Rhizoctonia solani isolate VC 268 dengan nilai query cover 100% dan identity 99,43%. Aktivitas penghambatan R. solani melalui uji dual culture bersama isolat C. freundii strain LMG 3246 dan P. aeruginosa strain FC1384 berkisar 57,06% dan 85,53% setelah 7 hari inkubasi, serta penghambatan perkecambahan sklerotia sebesar 100%. Temuan ini dapat menjadi dasar untuk aplikasi pengembangan biofertilizer dengan memanfaatkan potensi bakteri rhizosfer PGPR terhadap strategi pengendalian hayati penyakit hawar pelepah serta meningkatkan produktivitas padi melalui pemanfaatan bakteri rhizosfer sebagai agen biokontrol.
Co-Authors Abdi Dharma Achyani Achyani, Achyani Apriliansyah, Gusti Atika Permata Sari Ayu Safitri Bagas Rasid Sidik Elida Mardiah Elisa Nurnawati Erie Agusta, Erie Hary Widjajanti Hasanah, Khairatul Indah Indah Iqbar, Muhammad Khoirunnisa, Khosy Laila Hanum Muharni Muharni Musifa, Eva Mustafa Kamal Nita Aminasih Pragustiandi, Guntur Putri, Cyntia Sabarani Putri, Ferta Liza Rina Sri Kasiamdari Rizki, Muhammad Ali Azis Hasan Silvian, Trissa Talib, Ramanisa Muliani Tania, Alinda Warni, Mega Yadi Oktariansyah yulianti, nurhasanah