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All Journal International Journal of Public Health Science (IJPHS) Biology, Medicine, & Natural Product Chemistry At-Taqaddum Pharmascience Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Bina Generasi Jurnal Analis Kesehatan Journal of Dedicators Community AL-HAYAT: Journal of Biology and Applied Biology Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Indonesian Journal of Global Health research Jurnal Sains dan Edukasi Sains Anakes: Jurnal Ilmiah Analis Kesehatan Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas MAHESA : Malahayati Health Student Journal Jurnal Abdi Masyarakat Indonesia Jurnal Farmasetis GEMBIRA (Pengabdian Kepada Masyarakat) BIOGENIC: Jurnal Ilmiah Biologi Jurnal Pengabdian dan Pemberdayaan Kesehatan Media Penelitian dan Pengembangan Kesehatan Jurnal Pengemas Kesehatan
Sayekti, Fitria Diniah Janah
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EFEK NEFROPROTEKTIF MADU HUTAN TERHADAP GINJAL Rattus norvegicus YANG DIINDUKSI ASPARTAM BERDASARKAN GAMBARAN HISTOPATOLOGI Putri, Cahaya Anindya; Sayekti, Fitria Diniah Janah
Cendekia Journal of Pharmacy Vol 10, No 1 (2026): Cendekia Journal of Pharmacy
Publisher : Institut Teknologi Kesehatan Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/cjp.v10i1.285

Abstract

Aspartam merupakan pemanis sintetis yang sering digunakan pada produk makanan dan minuman seperti minuman bersoda, minuman berenergi, kopi instan, dan pada minuman berperisa. Aspartam yang dikonsumsi secara berlebihan dapat menimbbulkan perubahan  pada gambaran mikroskopis organ ginjal. Salah satu bahan alam yang memiliki efek nefroprotektif adalah madu yang mengandung flavonoid, yang berperan sebagai antioksidan. Penelitian ini dilakukan dengan metode eksperimental dengan tujuan untuk mengetahui efek nefroprotektof pada histopatologis organ ginjal tikus putih galur wistar yang telah diberikan madu hutan dengan konsentrasi 25%, 50%, dan 75% setelah diinduksi aspartam. Perlakuan dibagi menjadi 5 kelompok yaitu kelompok kontrol normal (K1), kelompok kontrol negatif (K2), kelompok pemberian aspartam dan madu hutan dengan konsentrasi 25% (K3), kelompok pemberian aspartam dan madu hutan dengan konsentrasi 50% (K4), kelompok pemberian aspartam dan madu hutan dengan konsentrasi 75% (K5). Pengamatan secara makroskopis didapatkan hasil bahwa tidak terdapat perbedaan warna ginjal, dimana pada kelompok perlakuan K1, K2, K3, K4, K5 berwarna coklat kemerahan, tekstur kenyal, dan ukuran yang berbeda-beda. Pada hasil pengamatan mikroskopis yang dianalisis menggunakan uji statistik One Way Anova dengan nilai sig. ? 0,05 yang berarti terdapat perbedaan bermakna rerata pada semua perlakuan. Berdasarkan uji lanjut Post Hoc Tukey didapatkan hasil konsentrasi madu hutan 75% paling efektif dalam memberikan efek nefroprotektif yaitu pada kelompok (K5).
Hubungan Viral Load HIV1-RNA terhadap Jumlah Trombosit Pada Pasien yang Terdeteksi HIV di Surabaya Ardhiyani, Ardhiyani; Sayekti, Fitria Diniah Janah
MAHESA : Malahayati Health Student Journal Vol 6, No 3 (2026): Volume 6 Nomor 3 (2026)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v6i3.21004

Abstract

ABSTRACT HIV (Human Immunodeficiency Virus) is a virus that attacks the immune system and can lead to AIDS. Thrombocytopenia, which is a reduction in platelet count, is frequently found in HIV patients and may impact their prognosis. This study aims to analyze the relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya. This study aims to determine the relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya Clinical Laboratory. This study uses an analytical observational design with a cross-sectional approach. The sample consists of 31 HIV patients who underwent HIV-1 RNA testing at Prodia Surabaya Laboratory from December 2024 to February 2025. Data on HIV-1 RNA viral load and platelet count were analyzed using Spearman’s statistical test. The results show that the majority of patients had an HIV-1 RNA viral load of 4.0 x 10¹ (51.61%) and normal platelet counts (83.88%). Normality tests indicated that both variables (viral load and platelet count) were not normally distributed (p 0.001). Spearman’s test yielded a correlation coefficient of -0.157 with a significance value of p = 0.400, indicating that there is no significant relationship between platelet count and HIV-1 RNA viral load. There is no significant relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya. Other factors such as nutritional status, antiretroviral therapy, and co-morbid infections may contribute to influencing platelet count. Keywords: HIV, Platelet, Thrombocytopenia, Viral Load.  ABSTRAK HIV (Human Immunodeficiency Virus) adalah virus yang menyerang sistem kekebalan tubuh dan dapat menyebabkan AIDS. Trombositopenia, yaitu penurunan jumlah trombosit dalam darah, sering kali ditemukan pada pasien HIV dan dapat mempengaruhi prognosis mereka. Penelitian ini bertujuan untuk menganalisis hubungan antara kadar trombosit dengan viral load HIV-1 RNA pada pasien HIV yang terdeteksi di Prodia Surabaya. Penelitian ini bertujuan untuk mengetahui hubungan antara kadar trombosit dengan viral load HIV-1 RNA pada pasien yang terdeteksi HIV di Laboratorium Klinik Prodia Surabaya. Penelitian ini menggunakan desain analitik observasional dengan pendekatan cross-sectional. Sampel penelitian terdiri dari 31 pasien HIV yang menjalani pemeriksaan HIV-1 RNA di Laboratorium Prodia Surabaya pada periode Desember 2024 hingga Februari 2025. Data viral load HIV-1 RNA dan kadar trombosit dianalisis dengan uji statistik Spearman. Hasil penelitian menunjukkan bahwa mayoritas pasien memiliki viral load HIV-1 RNA 4,0 x 10¹ (51,61%) dan kadar trombosit dalam batas normal (83,88%). Uji normalitas menunjukkan bahwa kedua variabel (viral load dan trombosit) tidak terdistribusi normal (p 0,001). Uji Spearman menghasilkan koefisien korelasi -0,157 dengan nilai signifikansi p = 0,400, yang mengindikasikan bahwa tidak terdapat hubungan yang signifikan antara kadar trombosit dan viral load HIV-1 RNA. Tidak terdapat hubungan yang signifikan antara kadar trombosit dan viral load HIV-1 RNA pada pasien yang terdeteksi HIV di Prodia Surabaya. Faktor-faktor lain seperti status gizi, terapi antiretroviral, dan infeksi ko-morbid mungkin berperan dalam mempengaruhi kadar trombosit. Kata Kunci: HIV, Trombosit, Trombositopenia, Viral Load.
Hepatotoxicity effect of alcoholic beverages on histology and IL-6 gene detection in Rattus norvegicus using polymerase chain reaction Sayekti, Fitria Diniah Janah; Dewangga, Vector Stephen; Qurrohman, Muhammad Taufiq
International Journal of Public Health Science (IJPHS) Vol 15, No 1: March 2026
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v15i1.27001

Abstract

The liver is an organ that has an important role in metabolic and detoxification processes. Alcohol consumption will cause metabolic disorders in the liver and can cause changes in histological structures. Interleukin-6 has a profound role in liver pathology and plays an important role in the body's defense mechanism, and contributes to the manifestation of tissue damage. This study was an experimental study using 20 Rattus norvegicus treated with 25% alcohol, and a normal treatment only given distilled water. Histological observations were carried out on each preparation in 5 fields of view. The hepatotoxicity effect of alcohol was observed based on the presence of cell degeneration and necrosis in histological preparations stained with hematoxylin eosin. IL-6 detection with polymerase chain reaction was analyzed qualitatively using electrophoresis. Based on the statistical test results, the sig. (2 tailed) of 0.00 < 0.05 so that it can be seen that there is a significant difference in histological results between the normal control group without treatment and the group induced by alcohol. The administration of alcoholic beverages with 25% ethanol content in vivo for 21 days causes necrosis and bleeding around the hepatocyte cells. IL-6 overview appeared to be stronger in the group with alcohol-induced drinking, it could indicate that giving alcohol causes a higher inflammatory response. This study provides scientific evidence that alcohol consumption significantly damages the liver. Further research can be conducted to increase the sample size and quantitatively assess IL-6 expression.
Detection of the Murine Double Minute2 (MDM2) gen in formalin-fixed tissue samples using PCR Sanita, Hanifah Velda; Sayekti, Fitria Diniah Janah
Media Penelitian dan Pengembangan Kesehatan Vol. 36 No. 1 (2026): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v36i1.3605

Abstract

Background: Tissue preservation is a crucial procedure in pathological studies to maintain cellular and structural integrity, commonly performed through formalin fixation. However, formalin fixation may cause DNA degradation, posing challenges for molecular analysis. Detection of cancer biomarker genes, such as Murine Double Minute 2 (MDM2), can be performed using Polymerase Chain Reaction (PCR), a technique for amplifying specific DNA fragments. Objective: This study aimed to determine the presence of the MDM2 gene in formalin-preserved tissues stored for different periods using PCR. Methods: A descriptive qualitative design with random sampling was employed. The study was conducted at the Anatomical Pathology and Molecular Biology Laboratories of Sekolah Tinggi Ilmu Kesehatan Nasional. Samples included 10 hair and nail tissues as controls, and formalin-fixed tissues stored for 2 days, 1 month, 2 months, and 8 years. DNA isolation was performed using the Geneaid gSYNC™ DNA Extraction Kit. DNA quality was assessed by 1.5% agarose gel electrophoresis, while concentration and purity were measured using a UV-Vis spectrophotometer. DNA concentrations ranged from 320–420 ng/µL, with purity ratios between 0.8 and 1.125. Results: The results showed that the MDM2 gene (178 bp amplicon) was detectable in tissues stored for up to 2 months but was not detected in tissues stored for 8 years. Conclusion: These findings indicate that storage duration affects the success of gene detection in formalin-fixed tissues.
Co-Authors Ahmad, Tasrif Andriansyah, Maulana Fikri Arabella, Exyananda Clorinda Ardhiyani, Ardhiyani Aryanti, Selvi Azzahra, Salsa Krisma Banowati, Annisa Cahyaningtyas, Priska Putri Dahlan Sitohang Damairia, Ema Rochayani Destiana Septianingrum Devi, Alvindo Thiananda Dewangga, Vector Stephen Fadhilla, Nadyah Saffana Fani, Sehan Astri Hari Saktiningsih Heryanti, Nabiilah Nuur Ainii Khoirudin, Ridwan Kirana Putri , Pramesti Magdalena Budi Verena P Marfianto, Alfian Marfianto, Alfian Mastuti Widi Lestari Mastuti Widi Lestari, Mastuti Widi Mawarni, Bunga Meidevita, Clarisa Muhammad Taufiq Qurrohman Muhammad Taufiq Qurrohman, Muhammad Taufiq Mukharomah, Katrine Adiansyah Dwi Nindiya Putri Damayanti Noviana, Sindi Octavianus, Nika Oktavia, Nadya Putri Pamilih, Anindya Khansa Putri Priyandari, Damar Ajeng Purnomo, Rochmad Agung Puspanegari, Ni Luh Larasati Putri, Cahaya Anindya Putri, Sherrin Karina Ramadhani, Yayi Aullia Rofifah, Karina Winda Sanita, Hanifah Velda Santosa, Lingka Elliesia Putri Sitohang, Dahlan Srikandini, Chris Tasrif Ahmad Tiara Anggraini Tunabenany, Gerardiana Selyn Tutik, Khofifah Indah Widyas Widyawati, Fransisca Wijayanti, Anastasia Elma Zidhan Hanafi