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All Journal International Journal of Public Health Science (IJPHS) At-Taqaddum Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Bina Generasi Jurnal Analis Kesehatan Journal of Dedicators Community AL-HAYAT: Journal of Biology and Applied Biology Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Indonesian Journal of Global Health research Jurnal Sains dan Edukasi Sains Anakes: Jurnal Ilmiah Analis Kesehatan Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas MAHESA : Malahayati Health Student Journal Jurnal Abdi Masyarakat Indonesia Jurnal Farmasetis GEMBIRA (Pengabdian Kepada Masyarakat) BIOGENIC: Jurnal Ilmiah Biologi Jurnal Pengabdian dan Pemberdayaan Kesehatan Media Penelitian dan Pengembangan Kesehatan Jurnal Pengemas Kesehatan
Sayekti, Fitria Diniah Janah
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Hepatotoxicity effect of alcoholic beverages on histology and IL-6 gene detection in Rattus norvegicus using polymerase chain reaction Sayekti, Fitria Diniah Janah; Dewangga, Vector Stephen; Qurrohman, Muhammad Taufiq
International Journal of Public Health Science (IJPHS) Vol 15, No 1: March 2026
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v15i1.27001

Abstract

The liver is an organ that has an important role in metabolic and detoxification processes. Alcohol consumption will cause metabolic disorders in the liver and can cause changes in histological structures. Interleukin-6 has a profound role in liver pathology and plays an important role in the body's defense mechanism, and contributes to the manifestation of tissue damage. This study was an experimental study using 20 Rattus norvegicus treated with 25% alcohol, and a normal treatment only given distilled water. Histological observations were carried out on each preparation in 5 fields of view. The hepatotoxicity effect of alcohol was observed based on the presence of cell degeneration and necrosis in histological preparations stained with hematoxylin eosin. IL-6 detection with polymerase chain reaction was analyzed qualitatively using electrophoresis. Based on the statistical test results, the sig. (2 tailed) of 0.00 < 0.05 so that it can be seen that there is a significant difference in histological results between the normal control group without treatment and the group induced by alcohol. The administration of alcoholic beverages with 25% ethanol content in vivo for 21 days causes necrosis and bleeding around the hepatocyte cells. IL-6 overview appeared to be stronger in the group with alcohol-induced drinking, it could indicate that giving alcohol causes a higher inflammatory response. This study provides scientific evidence that alcohol consumption significantly damages the liver. Further research can be conducted to increase the sample size and quantitatively assess IL-6 expression.
Detection of the Murine Double Minute2 (MDM2) gen in formalin-fixed tissue samples using PCR Sanita, Hanifah Velda; Sayekti, Fitria Diniah Janah
Media Penelitian dan Pengembangan Kesehatan Vol. 36 No. 1 (2026): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v36i1.3605

Abstract

Background: Tissue preservation is a crucial procedure in pathological studies to maintain cellular and structural integrity, commonly performed through formalin fixation. However, formalin fixation may cause DNA degradation, posing challenges for molecular analysis. Detection of cancer biomarker genes, such as Murine Double Minute 2 (MDM2), can be performed using Polymerase Chain Reaction (PCR), a technique for amplifying specific DNA fragments. Objective: This study aimed to determine the presence of the MDM2 gene in formalin-preserved tissues stored for different periods using PCR. Methods: A descriptive qualitative design with random sampling was employed. The study was conducted at the Anatomical Pathology and Molecular Biology Laboratories of Sekolah Tinggi Ilmu Kesehatan Nasional. Samples included 10 hair and nail tissues as controls, and formalin-fixed tissues stored for 2 days, 1 month, 2 months, and 8 years. DNA isolation was performed using the Geneaid gSYNC™ DNA Extraction Kit. DNA quality was assessed by 1.5% agarose gel electrophoresis, while concentration and purity were measured using a UV-Vis spectrophotometer. DNA concentrations ranged from 320–420 ng/µL, with purity ratios between 0.8 and 1.125. Results: The results showed that the MDM2 gene (178 bp amplicon) was detectable in tissues stored for up to 2 months but was not detected in tissues stored for 8 years. Conclusion: These findings indicate that storage duration affects the success of gene detection in formalin-fixed tissues.
HISTOPATOLOGI HEPAR Rattus norvegicus YANG DIBERI MADU PASKA INDUKSI MINUMAN BERENERGI Arabella, Exyananda Clorinda; Sayekti, Fitria Diniah Janah
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Minuman suplemen yang beredar dimasyarakat mengandung senyawa yang dapat menimbulkan adiksi dan hepatotoksik. Honeybee atau madu adalah bahan yang secara empiris dimanfaatkan di masyarakat yang memiliki berbagai kandungan senyawa metabolit sekunder. Penelitian ini berguna untuk melihat gambaran histologi hepar tikus putih yang diberi madu dengan konsentrasi 20% setelah perlakuan minuman berenergi. Hewan uji yang digunakan dalam penelitian sebanyak 25 ekor. Hasil makroskopis memperlihatkan bahwa organ hepar berwarna merah kecoklatan, tekstur kenyal dan ukuran cukup bervariasi namun tidak ada perbedaan antara kelompok. Hasil pengamatan mikroskopis memperlihatkan perubahan sel berupa degenerasi dan nekrosis. Uji ANOVA didapatkan nilai sig yaitu 0,393 (≥ 0,05), yang berarti pemberian madu menunjukkan perbaikan histopatologi hepar tikus putih setelah diberi tambahan minuman berenergi
Co-Authors Ahmad, Tasrif Andriansyah, Maulana Fikri Arabella, Exyananda Clorinda Ardhiyani, Ardhiyani Aryanti, Selvi Azzahra, Salsa Krisma Banowati, Annisa Cahyaningtyas, Priska Putri Dahlan Sitohang Damairia, Ema Rochayani Destiana Septianingrum Devi, Alvindo Thiananda Dewangga, Vector Stephen Fadhilla, Nadyah Saffana Fani, Sehan Astri Hari Saktiningsih Heryanti, Nabiilah Nuur Ainii Khoirudin, Ridwan Kirana Putri , Pramesti Magdalena Budi Verena P Marfianto, Alfian Marfianto, Alfian Mastuti Widi Lestari Mastuti Widi Lestari, Mastuti Widi Mawarni, Bunga Meidevita, Clarisa Muhammad Taufiq Qurrohman Muhammad Taufiq Qurrohman, Muhammad Taufiq Mukharomah, Katrine Adiansyah Dwi Nindiya Putri Damayanti Noviana, Sindi Octavianus, Nika Oktavia, Nadya Putri Pamilih, Anindya Khansa Putri Priyandari, Damar Ajeng Purnomo, Rochmad Agung Puspanegari, Ni Luh Larasati Putri, Cahaya Anindya Putri, Sherrin Karina Ramadhani, Yayi Aullia Rofifah, Karina Winda Sanita, Hanifah Velda Santosa, Lingka Elliesia Putri Sitohang, Dahlan Srikandini, Chris Tasrif Ahmad Tiara Anggraini Tunabenany, Gerardiana Selyn Tutik, Khofifah Indah Widyas Widyawati, Fransisca Zidhan Hanafi