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Stabilitas Bakteri Endofit Pseudomonas aeruginosa 2RWB2 Dan Pseudomonas aeruginosa 5BRB3 Dalam Formulasi Tepung Dengan Berbagai Bahan Tambahan Putri, Novita Pramahsari; Khaeruni, Andi; Ningsi Umar, Marnia; Santiaji, La Ode; Taufik, Muhammad; Satrah, Vit Neru; Sutariati, Gusti Ayu Kade; Wijayanto, Teguh
Agrium Vol 21 No 2 (2024)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v21i2.16782

Abstract

Bakteri endofit Pseudomonas aeruginosa 2RWB2 dan P. aeruginosa 5BRB3 terbukti efektif mengendalikan Phytophthora palmivora pada persemaian. Agar aplikasi agens hayati  dapat bertahan lama diperlukan suatu formula yang mampu mempertahankan viabilitas dan daya hambat serta kemudahkan penggunaannya. Penelitian bertujuan untuk mengetahui jenis formulasi tepung dan bahan tambahan yang memberikan pengaruh terbaik terhadap viabilitas dan daya hambat bakteri endofit Pseudomonas aeruginosa 2RWB2 dan P. aeruginosa 5BRB3 terhadap P. palmivora. Penelitian dilaksanakan di Laboratorium Proteksi Tanaman Unit Fitopatologi, Fakultas Pertanian Universitas Halu Oleo Kendari. Penelitian disusun menggunakan Rancangan acak lengkap dengan 6 perlakuan formula tepung dan bahan tambahan yang berbeda. Setiap perlakuan diulang sebanyak 3 kali dan dilaksanakan secara paralel dengan dua jenis bakteri endofit yaitu: P. aeruginosa 2RWB2 dan P. aeruginosa 5BRB3. Data dianalisis dengan sidik ragam dan apabila terdapat pengaruh nyata, maka dilanjutkan dengan Uji Jarak Berganda Duncan. Hasil penelitian menunjukkan bahwa tepung talk dan tepung tapioka dapat berfungsi sebagai bahan dasar dalam formulasi agens hayati bakteri endofit P. aeruginosa 2RWB2 dan P. aeruginosa 5BRB3. Formulasi tepung tapioka P6 dengan penambahan pepton memiliki kemampuan terbaik dalam meningkatkan viabilitas bakteri endofit P. aeruginosa 2RWB2 yaitu sebesar 8,34 x 108 CFU/g pada 8 MSI, formulasi P3 memiliki kemampuan daya hambat terbaik yaitu sebesar 87,78% dengan masa simpan formulasi 10 MSI. Sedangkan tepung tapioka P5 memiliki kemampuan terbaik dalam meningkatkan viabilitas bakteri endofit P. aeruginosa 5BRB3 yaitu sebesar 8,14 x 108 CFU/g pada 8 MSI, formulasi P6 memiliki kemampuan daya hambat terbaik yaitu sebesar 88,89% dengan masa simpan formulasi 12 MSI.
ANALISIS JARINGAN KOMUNIKSI PADA PENGEMBANGAN PERTANIAN ORGANIK DI KOTA KENDARI Purwanti, Rahayu Endah; Dima, Darsilan; Satrah, Vit Neru; Yusriadin, Yusriadin; Syarni, Pertiwi; Padangaran, Normal Bivariant
Jurnal Ilmiah Inovasi dan Komunikasi Pembangunan Pertanian Vol. 4 No. 2 (2025)
Publisher : Department of Agricultural Extension, Faculty of Agriculture, Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/jiikpp.v4i2.56

Abstract

The issue of sustainability is currently a very important issue in agricultural development. Organic farming is an application of a sustainable agricultural system. The back to nature lifestyle has become a trend for some people. However, the phenomenon encountered in the field is that there are still many organic farmers who have not fully implemented organic farming cultivation techniques in accordance with the Indonesian National Standard (SNI) issued by the Indonesian National Standardization Agency (BSN) and the adoption of innovations also seems slow. This study was conducted to describe the tendency of communication network patterns in farmer women's groups so as to accelerate the process of diffusion of innovations related to the development of organic farming in Kambu District, Kendari City. The specific objectives were: (1) analyzing the role of each key informant; (2) knowing the pattern of communication networks formed in organic farming development activities. This research is a survey research using quantitative analysis. The research location is in Kambu Sub-district, Kendari City, Southeast Sulawesi Province. Data collection was conducted through surveys, in-depth interviews, focused discussions and observations. Sample determination in this study is by census. Data analysis was descriptive. The results obtained in this study are that in the KWT Manggarai communication network there are three cliques in the form of fingers centered on one individual and spread to members. The specific functions found in the KWT Manggarai communication network are star, opinion leader, liaison officer, bridge, and no isolate. The average degree of connectedness in the KWT Manggarai communication network system is 21.33 and is classified as very good.