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All Journal Jurnal Ilmu Lingkungan Jurnal Riset Kimia JURNAL INTEGRASI PROSES Jurnal SOLMA Jurnal Kimia Terapan Indonesia Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Akta Kimia Indonesia Jurnal Saintek Lahan Kering Indonesia Journal of Halal Agroprimatech Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Stannum : Jurnal Sains dan Terapan Kimia BIO-EDU: Jurnal Pendidikan Biologi Molluca Journal of Chemistry Education (MJoCE) BERNAS: Jurnal Pengabdian Kepada Masyarakat Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology International Journal Of Science, Technology & Management (IJSTM) Hydrogen: Jurnal Kependidikan Kimia Jurnal Inovasi Penelitian dan Pengabdian Masyarakat Livestock and Animal Research Journal of Chemical Science and Application
Kolo, Maria Magdalena
Program Studi Kimia, Fakultas Pertanian, Sains Dan Kesehatan, Universitas Timor, NTT, Indonesia
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Journal : Journal of Tropical Animal Science and Technology

 1 
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

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Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
Co-Authors Adolvina Kono Agustina Rosalia Saka Agustinus Akoit Alfred Nubatonis Azor Y Tefa Azor Yulianus Tefa Batu, Matius S Batu, Matius Stafenus Bira, Gerson Frans Bria, Deseriana Bria, Emilia Juliyanti Cornelis Astridino Nitbani Deni Yonathan Lulu Edi, Eduardus Eduardus Yosef Neonbeni Efieno Maria Paulo Soares Eka Maria Sanit Elisa M. Thaal Emerensiana Naes Febrya Christin Handayani Buan Fransiska Rika Fredy Kurniawan Funan, Selviana Ermalinda Gelyaman, Gebhardus Djugian Gerson Frans Bira Gradiana Yosefa Nana Hilarius Y Sikone Jefry Presson jefry Presson Kia, Kristoforus W Korbafo, Elisabeth Lukas Pardosi Mading, Ridwan Mahardika Putra Purba Manek, Seprianus Septian Maria Fridolina Taek Maria Imaculata Mali Maria Rosanti Bani Mata, Meri Halsiana Matius Stefanus Batu Mere, Jenrigo Klaumegio Metriana Matilda Taus N. M. Obenu Naisau, Mikhael Rifantus Noviana Getrudis Abi Nugraha K. F. Dethan Oktovianus R Nahak Paulus Klau Tahuk Pieter Jackson Lopez Dethan Regina Seran Risna Erni Yati Adu Saka, Agustina Rosalia Sefrinus Maria Dolfi Kolo Sio Stefanus, Sio Sipayung, Boanerges Putra Taus, Jonisius Tea, Marselina Theresia Djue Tse, Anastasia Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Ivana Kedang Yuni Sine Zofar Agluis Banunaek