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Journal : Planta Tropika

Phylogenetic Relationships of Nine Cultivars of Strawberries (Fragaria spp.) Based on Anatomical and Morphological Characters Kasiamdari, Rina Sri; Aristya, Ganies Riza; Inayati, Evi
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 5, No 2 (2017)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2017.072.116-126

Abstract

Strawberry cultivation in Indonesia is centred at Citrus and Subtropical Fruit Research Institute, which collects different cultivars of Fragaria spp. Information on classification based on phenotypic characters of Fragaria spp. has not been studied as a whole. The purpose of the present study was to identify and determine phylogenic relationships of nine cultivars of strawberries based on the anatomical and morphological characters. The present study identified morphologically nine strawberry cultivars by referring to the IPGRI (1986) and the UPOV (2012) and Hofer et al. (2012). Anatomical preparations of roots, stems and leaves were made by the embedding method. Phylogenetic relationships were determined using MVSP software with UPGMA algorithm through the Gower General Similarity Coefficient method. Subsequently, the principal components were analyzed using the Euclidian Biplots algorithm depicted in a Scatter plot. Results showed that there were 37 anatomical and morphological characters that play a role in the characterization of nine cultivars (25 synthetic characters and 12 diagnostics characters) where the shape of the fruit and type of attachment of the flowers were the main characters distinguishing among the nine cultivars studied. The phylogenetic relationship produced two clusters with 79% similarity index. The closely related cultivars were Festival and Rosa Linda with a similarity index of 94%, and Earlibrite and Aerut with a similarity index of 86%
DETECTION AND IDENTIFICATION OF POLYMORPHISM IN MUTANT STRAWBERRY (FRAGARIA SPP.) PLANTS BASED ON CLEAVED AMPLIFIED POLYMORPHIC SEQUENCES MOLECULAR MARKERS Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Detection and Identification of Polymorphism in Mutant Strawberry (Fragaria spp.) Plants Based on Cleaved Amplified Polymorphic Sequences Molecular Markers Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Co-Authors Abdul Razaq Chasani, Abdul Razaq Abiyyi, Muhammad Hawi Qabus Afifah, Nasywa Kamila Agus Setiawan Akbar, Ananda Malik Almira Syafataya Amadeus, Sovann Ana Susianti Ani Widiastuti, Ani Aries Bagus Sasongko Aurantika, Rindu Ayundai, Melin Ayundai, Melin Azzahira, Faiha Azka Bartolomius Renaldy Bening Larasati Budi S Daryono BUDI SETIADI DARYONO Chalvia Zuyyina Christophorus Ardhito Haryo Dwinanda Dea Febiansi Dewi, Umi Rosita Dian Astriana Dimas, Abimanyu Dita Yulistiani Dyanita, Anissa Cahyadevi Elva Maulida Khozana Fadhlurrohman, Galih Dzaky Fajar Sofyantoro Fauzana Putri Febri Yuda Kurniawan Galang Riswi Dyatama Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hevi Wihadmadyatami Hidayat, Madyan Akmal Himawan Masyhuri Inayati, Evi Indra Lesmana Ishana, Aurelia Araya Karmilah, Karmilah Kusnah Dian Prasiwi Latifah, Vida Rahma Lisna Hidayati Mif Ardianata Pratama Putra Muhammad Mufid Al-Dayyan Muhammada, Khusnus Syifa Muliawati, Adina Nainggolan, Ananto Puradi Nasywa Kamila Afifah Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nugroho, Rifat Rizki Adi Nugroho Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putri, Fauzana Putri, Fauzana Ramadhani, Gilang Afif Setya Rezika Alyza Rifa Ayuningsih Rina Sri Kasiamdari Rispaini Nur Aziza Risydah, Fiqiha Rosyidatul Khoiroh Safitri, Della Thasya Liona Salsabila, Sophia Sholihatun Nissa Sigit Dwi Maryanto Sofyantoro, Fajar Sritanjung, Opi Sutikno S Syafei, Dio Rachmaputra Tasyarofa, Alya Teguh Budipitojo Triska Ayu Nurwijayanti Tuty Arisuryanti, Tuty Ulfa, Siti Zakia Uswatun Mujahidah Utami, Mia Widiastuti, Ani Widyasari, Adristi Shafa Wulandari, Mutiara Tri Yuanita Rachmawati Yuda, Khalid Abdillah Abdurahman Yusuf, Adib Fakhruddin