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All Journal Jurnal Ilmu Pertanian Indonesia AGRIKA Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Pelita Perkebunan Jurnal Agroekoteknologi Agriprima : Journal of Applied Agricultural Sciences BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika JURNAL AGROHITA Jurnal Ilmiah RESPATI Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agroteknika Jurnal Agrium Jurnal Agrotropika Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi International Journal on Food, Agriculture and Natural Resources (IJFANRES) Batara Wisnu : Indonesian Journal of Community Services Journal of Farming and Agriculture Agrocentrum
Windriyanti, Wiwin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Efficacy of Endophytic Bacteria as Entomopathogens against Spodoptera frugiperda (Lepidotera: Noctuidae) on Corn (Zea mays L.) Kurniawati Kurniawati; Arika Purnawati; Wiwin Windriyanti
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.1040-1050

Abstract

The fall armyworm (Spodoptera frugiperda) poses a problem for corn plants (Zea mays L.). The use of endophytic bacteria Bacillus sp. as an entomopathogen is expected to control the S. frugiperda pest. This study aims to determine the effective concentration of the bacteria and the active compounds produced by Bacillus sp. strain Bth 22 in controlling S. frugiperda. The research was conducted from August to October 2024 at the Plant Health Laboratory of the Faculty of Agriculture, UPN "Veteran" East Java, and the Airlangga Research Hub in Surabaya. The study was designed using a completely randomized design (CRD) with bacterial concentration treatments of 0% (control), 10%, 15%, 20%, 25%, 30%, and 35%, repeated five times. Observational parameters included mortality rate, number of pupae and imago formed, as well as the mechanisms and compounds produced by Bacillus bacteria based on HPLC and FT-IR tests. The application of Bacillus sp. Bth-22 affected the mortality parameters, the number of pupae formed, and the number of imago formed. The Bacillus sp. Bth-22 bacteria produced metabolites in the form of hydrocarbon derivatives and amide group compounds, disrupting metabolism and digestion, leading to the mortality of S. frugiperda. Keywords: Entomopathogen, Metabolite compounds, Spodoptera frugiperda, Zea mays 
Diversity and Low Parasitism of Egg Parasitoids Associated with Maize (Zea mays) in Madura, Indonesia Panca Ayu Virgirl; Wiwin Windriyanti; Ramadhani Mahendra Kusuma
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.832-840

Abstract

This study aimed to explore and identify egg parasitoids associated with maize (Zea mays) in Keleyan Village, Bangkalan Regency, Madura. Field observations were conducted during both vegetative and generative growth stages using a direct survey method. Eggs of lepidopteran pests were collected and reared under laboratory conditions to observe the emergence of adult parasitoids. The results showed that only one genus of egg parasitoid was found, namely Trichogramma spp., which parasitized the eggs of Spodoptera frugiperda at a parasitism rate of 17.3%. This rate was low for a biological control program. These findings indicate that the presence of natural parasitoids in the study area was not sufficiently effective in suppressing pest populations under natural conditions. Nevertheless, Trichogramma spp. still shows potential as a biological control agent, particularly if supported by augmentative or conservation-based approaches. This results can be used as foundation for the development of ecological-based environmentally friendly, and sustainable pest control strategies in lowland maize cultivation systems, especially in the Madura region.
Microclimate Modification through Water Misting Increase Natural Enemy Insect Richness in a Cocoa Agroecosystem Annisa Damayanti; Wiwin Windriyanti; Ramadhani Mahendra Kusuma
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17799

Abstract

This study aimed to analyze the influence of abiotic factors on the diversity of natural enemies in cocoa fields. The study was conducted from January to March 2025. Various traps were used, including yellow traps, light traps, pitfall traps, and visual observations. Identification showed that cocoa fields with misting applications had a higher number of natural enemies (1,052 individuals) compared without misting fields (658 individuals). The application of misting resulted in higher diversity indices (H'), richness (R'), and dominance (C'), as well as lower evenness (E') compared to untreated fields. These findings suggest that the misting system creates a more favorable microhabitat for natural enemy diversity, thus potentially strengthening the IPM approach in cocoa cultivation. Interestingly, the analysis showed that temperature had a significance value (sig) > 0.05, indicating that temperature did not show a significant correlation with the increase in the abundance of natural enemies, while humidity has a significance value (sig) < 0.05, which shows that humidity has a significant correlation, although statistically relatively small, with the increase in the abundance of natural enemies.
Karakterisasi Senyawa Pestisida pada Nanopartikel Ekstrak Bawang Putih (Allium Sativum L.) dan Uji Mortalitas Terhadap Ulat Grayak (Spodoptera Litura F.) di Laboratorium Wahyu Ramadhani Putra Josua; Wiwin Windriyanti; Ramadhani Mahendra Kusuma
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18441

Abstract

This study aims to characterize the pesticide compounds in garlic (Allium sativum L.) extract nanoparticles and test their mortality effectiveness against armyworms (Spodoptera litura F.) in the laboratory. Nanoparticle synthesis was carried out using the ionic gelation method with chitosan-TPP, which was subsequently characterized using PSA, SEM, FT-IR, and GC-MS. The characterization results showed a particle size of 930.52 nm with a homogeneous distribution (PDI 0.147). GC-MS analysis successfully identified the main bioactive organosulfur compounds, namely diallyl disulfide (20.72%), diallyl trisulfide (14.16%), and diallyl tetrasulfide (5.79%). Bioactivity testing using the sandwich method at five concentration variations (0-1%) showed the highest mortality reaching 70% at a 1% concentration. Probit analysis yielded values of LC₅₀ 1.116% and LC₉₀ 1.720%, as well as LT₅₀ 5.117 days and LT₉₀ 9.893 days. Statistical test results indicated a significant difference (p<0.05) between all treatments and the control group. Thus, it can be concluded that the garlic extract nanoparticle formulation has the potential to be an effective nano-bioinsecticide, with a mode of action that is dose-dependent and time-dependent in controlling S. litura larvae.
Co-Authors Aldi Kurniansyah Ali, Haidar Alifta Wiranthi Alifta Wiranthi Annisa Damayanti Ardiansyah, Yoyong Ari Wijayanti Arika Purnawati Barna Deta Cristin Naftaly Choerunnisa, Agustya Salma Dita Megasari Dwi Afrian Dwi Afrian Dwi Afrian Dwi Afrian Dwi Suci Rahayu Fadila Suryandika Ferdianto Eko Wicaksono Fitri Wijayanti Fitria Yuliasmara Fitriyani, Novi Nur Haidar Fari Aditya Harsiyanti, Harsiyanti Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto I GEDE SWIBAWA, I GEDE Iis Purnamawati Indah Anita Sari Indah Nathasya, Rachmawaty Indrawan, Arga Dwi Iwan Kresna Setiadi Khafi, Achmad Muhaimin Nur Khasanah, Nurdiah Kurniawati Kurniawati Mahendra Kusuma, Ramadhani Maroeto Maroeto Mira Eka Nursandi Mochamad Arifin Muhammad Arif Wahyudin Muhammad Fatir Ma&#039;ruf Musarofa, Musarofa Ningrum, Linda Wige Nisa, Isnaini Lailatun Noni Rahmadhini Novi Nafisah Najmi Novita Endah Ariani Novita Endah Ariani Nur Supriyadi, Yudi Panca Ayu Virgirl Pangesti Nugrahani Penta Suryaminarsih Penta SURYAMINARSIH Pratista, Ariq Dies Puji Lestari Tarigan Purnomo Edi Sasongko Putri, Fanny Etrisya Putri, Vadila Mulia Ramadhan, Panji Bagus Ramadhani Mahendra Kusuma Ramadhani Mahendra Kusuma Ramadhani Mahendra Kusuma Ratri, Tesha Lutfika Reksiana, Catur Putri Enggit Safira Rizka Lestari Salfi Mauludhiea Firli Shavira Dwi Livita Reza Sholikha, Annida Nur Rifatus Sisko Budianto Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Titik Nur Aeny Tri MUJOKO Ukhrowi, Syerlina Titis Muawanah Wahyu Ramadhani Putra Josua Wahyu Santoso Wanti Mindari Widajati, Wiludjeng Widayati, Wiludjeng Widayati, Wilujeng Wijayanto, Mohamad Adi Wiludjeng Widajati Wiludjeng Widayati Yenny WURYANDARI YUDI NUR SUPRIADI