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All Journal Jurnal Ilmu Pertanian Indonesia AGRIKA Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Pelita Perkebunan Jurnal Agroekoteknologi Agriprima : Journal of Applied Agricultural Sciences BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika JURNAL AGROHITA Jurnal Ilmiah RESPATI Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agroteknika Jurnal Agrium Jurnal Agrotropika Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi International Journal on Food, Agriculture and Natural Resources (IJFANRES) Batara Wisnu : Indonesian Journal of Community Services EduInovasi: Journal of Basic Educational Studies Journal of Farming and Agriculture Agrocentrum
Windriyanti, Wiwin
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Karakterisasi Senyawa Pestisida pada Nanopartikel Ekstrak Bawang Putih (Allium Sativum L.) dan Uji Mortalitas Terhadap Ulat Grayak (Spodoptera Litura F.) di Laboratorium Josua, Wahyu Ramadhani Putra; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18441

Abstract

This study aims to characterize the pesticide compounds in garlic (Allium sativum L.) extract nanoparticles and test their mortality effectiveness against armyworms (Spodoptera litura F.) in the laboratory. Nanoparticle synthesis was carried out using the ionic gelation method with chitosan-TPP, which was subsequently characterized using PSA, SEM, FT-IR, and GC-MS. The characterization results showed a particle size of 930.52 nm with a homogeneous distribution (PDI 0.147). GC-MS analysis successfully identified the main bioactive organosulfur compounds, namely diallyl disulfide (20.72%), diallyl trisulfide (14.16%), and diallyl tetrasulfide (5.79%). Bioactivity testing using the sandwich method at five concentration variations (0-1%) showed the highest mortality reaching 70% at a 1% concentration. Probit analysis yielded values of LC₅₀ 1.116% and LC₉₀ 1.720%, as well as LT₅₀ 5.117 days and LT₉₀ 9.893 days. Statistical test results indicated a significant difference (p<0.05) between all treatments and the control group. Thus, it can be concluded that the garlic extract nanoparticle formulation has the potential to be an effective nano-bioinsecticide, with a mode of action that is dose-dependent and time-dependent in controlling S. litura larvae.
The Potency of Streptomyces spp. from Shallot Land as Entomopathogen of Onion Caterpillar Pest Spodoptera exigua Pratista, Ariq Dies; Windriyanti, Wiwin; Suryaminarsih, Penta; Mujoko, Tri; Indrawan, Arga Dwi; Harsiyanti, Harsiyanti
International Journal on Food, Agriculture and Natural Resources Vol 6, No 4 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i4.569

Abstract

Spodoptera exigua is a major pest of shallots (Allium ascalonicum L.), capable of causing up to 100% yield loss if uncontrolled. Streptomyces sp. is a soil-dwelling Actinomycetes bacterium that can be used to control S. exigua. This study aimed to determine the potential of Streptomyces spp. isolates from shallot fields as an entomopathogen against S. exigua. The research employed a Factorial Complete Randomized Design (CRD). The first factor consisted of one Streptomyces spp. isolate obtained from the Pare-Kediri region (BMP) and three isolates from Sidera-Palu (BMS1, BMS2, and BMS3). The second factor was the concentration of the Streptomyces sp. suspension consist of 0%; 5%; 10%; and 15% with three replication per treatment (48 units total). Data on larval mortality and damage intensity were analyzed using RStudio via ANOVA and DMRT (α=5%). The BMP (15%) treatment demonstrated the highest efficacy, achieving 76.67% larval mortality while maintaining the lowest damage intensity of 0.20% on shallot plants. These results highlight BMP isolate at 15% concentration as the most promising biocontrol agent and support its further field evaluation as a sustainable alternative to chemical insecticides.
The Effect of Insect Flour on The Growth Media of Beauveria bassiana in Controlling the Pupa Stage of Bactrocera carambolae Choerunnisa, Agustya Salma; Rahmadhini, Noni; Windriyanti, Wiwin
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17713

Abstract

Background: One effective method for controlling Bactrocera carambolae is the use of Beauveria bassiana. However, a common challenge is the decline in the conidial quality and virulence due to the lack of chitin and protein sources. The insect Tenebrio mollitor and the pupal casings of Hermetia illucens are rich in chitin and have the potential to enhance the growth medium. Methods: The study used RAKF consisting of 3 factors and 3 replications. The first factor was the Type of Flour (T) consisting of T1: T. mollitor and T2: H. illucens. The second factor was Flour Concentration (K), which consisted of K1: 0.5% K2: 1% and K3: 1.5%. The third factor was Drying Temperature (S), of S1: 1000C S2: 1100C and S3: 1200C. Results: The results of the study show that the treatment caused symptoms of infection in the form of mycelium growth on the pupal cuticle and abnormalities in the emergence imago. The best combination is T2K2S2 (1% H. illucens flour at 1100C) which gives a mortality rate up to 76.7%, pupal duration of 9.3 days, and viability rate of 92.2%. Conclusions: The combination of H. illucens flour at a concentration of 1% and a drying temperature of 1100C is quite effective in increasing the ability of B. bassiana in controlling B. carambolae pupae in vitro.
Predasi dan Respons Fungsional Lima Spesies Coccinellidae sebagai Predator Potensial Wereng Batang Coklat (Nilaparvata lugens) Karim, Fatikhul; Windriyanti, Wiwin; Nirwanto, Hery
EduInovasi:  Journal of Basic Educational Studies Vol. 6 No. 1 (2026): EduInovasi:  Journal of Basic Educational Studies
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Brown planthopper (Nilaparvata lugens) is a major rice pest that can significantly reduce yields, even leading to crop failure, therefore a biological control strategy is needed by utilizing natural predators.This study tested the predation ability and functional responsse of five Coccinellidae species (Micraspis lineata, Micraspis discolor, Cheilomenes sexmaculata, Coccinella transversalis, and Coelophora inaequalis) against brown planthopper imago at five levels of prey density using a factorial Completely Randomized Design with 48 hour observation. This study analyzed the ability to prey, predation rate, attack rate, handling time, and the type of functional responsse through regression based on Holling Disc Equation modeling. The results showed significant differences between species (p < 0.05) with C. transversalis being the most efficient predator with the highest attack rate (0.6861) and the lowest handling time (0.0311), followed by M. lineata (a = 0.6615; Th = 0.0798), while C. inaequalis was the predator with the lowest effectiveness. All species showed a type III functional responsse with the quadratic model having the highest R². These results indicate that C. transversalis and M. lineata are potential predators as biological control agents for brown planthopper in rice agroecosystems.
Efficacy of Endophytic Bacteria as Entomopathogens against Spodoptera frugiperda (Lepidotera: Noctuidae) on Corn (Zea mays L.) Kurniawati Kurniawati; Arika Purnawati; Wiwin Windriyanti
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.1040-1050

Abstract

The fall armyworm (Spodoptera frugiperda) poses a problem for corn plants (Zea mays L.). The use of endophytic bacteria Bacillus sp. as an entomopathogen is expected to control the S. frugiperda pest. This study aims to determine the effective concentration of the bacteria and the active compounds produced by Bacillus sp. strain Bth 22 in controlling S. frugiperda. The research was conducted from August to October 2024 at the Plant Health Laboratory of the Faculty of Agriculture, UPN "Veteran" East Java, and the Airlangga Research Hub in Surabaya. The study was designed using a completely randomized design (CRD) with bacterial concentration treatments of 0% (control), 10%, 15%, 20%, 25%, 30%, and 35%, repeated five times. Observational parameters included mortality rate, number of pupae and imago formed, as well as the mechanisms and compounds produced by Bacillus bacteria based on HPLC and FT-IR tests. The application of Bacillus sp. Bth-22 affected the mortality parameters, the number of pupae formed, and the number of imago formed. The Bacillus sp. Bth-22 bacteria produced metabolites in the form of hydrocarbon derivatives and amide group compounds, disrupting metabolism and digestion, leading to the mortality of S. frugiperda. Keywords: Entomopathogen, Metabolite compounds, Spodoptera frugiperda, Zea mays 
Diversity and Low Parasitism of Egg Parasitoids Associated with Maize (Zea mays) in Madura, Indonesia Panca Ayu Virgirl; Wiwin Windriyanti; Ramadhani Mahendra Kusuma
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.832-840

Abstract

This study aimed to explore and identify egg parasitoids associated with maize (Zea mays) in Keleyan Village, Bangkalan Regency, Madura. Field observations were conducted during both vegetative and generative growth stages using a direct survey method. Eggs of lepidopteran pests were collected and reared under laboratory conditions to observe the emergence of adult parasitoids. The results showed that only one genus of egg parasitoid was found, namely Trichogramma spp., which parasitized the eggs of Spodoptera frugiperda at a parasitism rate of 17.3%. This rate was low for a biological control program. These findings indicate that the presence of natural parasitoids in the study area was not sufficiently effective in suppressing pest populations under natural conditions. Nevertheless, Trichogramma spp. still shows potential as a biological control agent, particularly if supported by augmentative or conservation-based approaches. This results can be used as foundation for the development of ecological-based environmentally friendly, and sustainable pest control strategies in lowland maize cultivation systems, especially in the Madura region.
Co-Authors Aldi Kurniansyah Ali, Haidar Alifta Wiranthi Alifta Wiranthi Anestesia Hayyu Palupi, Mandayu Ardiansyah, Yoyong Ari Wijayanti Arika Purnawati Barna Deta Cristin Naftaly Choerunnisa, Agustya Salma Damayanti, Annisa Dita Megasari Dwi Afrian Dwi Afrian Dwi Afrian Dwi Afrian Dwi Elvira, Nolla Dwi Suci Rahayu Fadila Suryandika Ferdianto Eko Wicaksono Fitri Wijayanti Fitria Yuliasmara Fitriyani, Novi Nur Haidar Fari Aditya Harsiyanti, Harsiyanti Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto, Hery I GEDE SWIBAWA, I GEDE Iis Purnamawati Indah Anita Sari Indah Nathasya, Rachmawaty Indrawan, Arga Dwi Iwan Kresna Setiadi Josua, Wahyu Ramadhani Putra Karim, Fatikhul Khafi, Achmad Muhaimin Nur Khasanah, Nurdiah Kurniawati Kurniawati Mahendra Kusuma, Ramadhani Maroeto Maroeto Mira Eka Nursandi Mochamad Arifin Muhammad Arif Wahyudin Muhammad Fatir Ma&#039;ruf Musarofa, Musarofa Ningrum, Linda Wige Nisa, Isnaini Lailatun Noni Rahmadhini Novi Nafisah Najmi Novita Endah Ariani Novita Endah Ariani Nur Supriyadi, Yudi Panca Ayu Virgirl Pangesti Nugrahani Penta Suryaminarsih Penta SURYAMINARSIH Pratista, Ariq Dies Puji Lestari Tarigan Purnomo Edi Sasongko Putri, Fanny Etrisya Putri, Vadila Mulia Qurrotu Aini, Anisah Ramadhan, Panji Bagus Ramadhani Mahendra Kusuma Ramadhani Mahendra Kusuma Ratri, Tesha Lutfika Reksiana, Catur Putri Enggit Safira Rizka Lestari Salfi Mauludhiea Firli Shavira Dwi Livita Reza Sholikha, Annida Nur Rifatus Sisko Budianto Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Syaiful Khoiri Titik Nur Aeny Tri MUJOKO Ukhrowi, Syerlina Titis Muawanah Wahyu Santoso Wanti Mindari Widajati, Wiludjeng Widayati, Wiludjeng Widayati, Wilujeng Wijayanto, Mohamad Adi Wiludjeng Widajati Wiludjeng Widayati Yenny WURYANDARI YUDI NUR SUPRIADI