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All Journal Biosfer: Jurnal Tadris Biologi Asian Journal of Agriculture Best Journal (Biology Education, Sains and Technology) JURNAL BIOSHELL Bioscientist : Jurnal Ilmiah Biologi Continuous Education : Journal of Science and Research BIO-CONS: Jurnal Biologi dan Konservasi Riwayat: Educational Journal of History and Humanities Jurnal Esabi (Jurnal Edukasi dan Sains Biologi)
Daulae, Abdul Hakim
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Public Health Social Sciences Other

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In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal HARAHAP, FAUZIYAH; SIREGAR, ANITA RASUNA SARI; IDRAMSA, IDRAMSA; SURIANI, CICIK; EDI, SYAHMI; PANGGABEAN, NURUL HUDA; DAULAE, ABDUL HAKIM; POHAN, SELVIA DEWI; TANJUNG, AURA ALBIZIA; PERTIWI, SUFINA INTAN; KAIRANI, ANITA
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100130

Abstract

Abstract. Harahap F, Siregar ARS, Idramsa, Suriani C, Edi S, Panggabean NH, Daulae AH, Pohan SD, Tanjung AA, Pertiwi SI, Kairani A. 2026. In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal. Asian J Agric 10 (1): g100130. https://doi.org/10.13057/asianjagric/g100130. Cattleya sp. is an ornamental orchid whose seeds lack of endosperm, making natural germination and propagation difficult. In vitro culture using Protocorm-Like Bodies (PLBs) offers an alternative for mass propagation. This study aimed to evaluate the effects of coconut water and activated charcoal on the in vitro regeneration of Cattleya sp. PLBs. A completely randomized design was employed with varying concentrations of coconut water (0, 5, and 10%) and activated charcoal (0, 0.1, 0.2, and 0.3 g/L). Morphological parameters observed included time to shoot emergence, number of leaves, shoots, and roots, as well as shoot color. Data were analyzed using two-way ANOVA. The results showed that coconut water significantly accelerated shoot emergence with 10% of coconut water producing the fastest response (12 days after treatment). Activated charcoal enhanced shoot and leaf formation with 0.3 g/L, producing the highest number of shoot and leaf. Root formation was optimal at lower charcoal concentrations. Overall, the combination of coconut water and activated charcoal significantly influenced PLBS growth and regeneration. These findings indicate that appropriate combinations of organic additives can optimize in vitro regeneration of Cattleya sp. and support efficient orchid propagation.
Co-Authors Afifah, Fadilah Arda, Gladys Mutia Ashar Hasairin Asri, Elita CICIK SURIANI Dawolo, Alfandi Putra Jaya Fauziyah Harahap Harahap, Salamah Hasanah, Dwi Hasruddin Idramsa Idramsa, Idramsa Kairani, Anita Melva Silitonga Mufti Sudibyo, Mufti Muhammad Fariz Nadhilla, Annisa Nursamsu Nursamsu Nusyirwan Nusyirwan Panggabean, Nurul Huda Panggabean, Syakilah Kurnia Fahmi PERTIWI, SUFINA INTAN Puji Prastowo, Puji Putri, Dita Aisyah Selvia Dewi Pohan Sembiring, Gres Anjelia Sihombing, Josephine Gloria Margareth Simbolon, Tirini Gabriella Veronica Siregar, Anita Rasuna Sari Syahmi Edi Tanjung, Aura Albizia