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All Journal JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biologi Tropis BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JURNAL PENDIDIKAN TAMBUSAI Jurnal Mangifera Edu Jurnal Review Pendidikan dan Pengajaran (JRPP) Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi SOSIOEDUKASI : JURNAL ILMIAH ILMU PENDIDIKAN DAN SOSIAL Spizaetus: Jurnal Biologi dan Pendidikan Biologi JURNAL BIOSHELL Bioscientist : Jurnal Ilmiah Biologi TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Bioed : Jurnal Pendidikan Biologi Jurnal Pijar MIPA Pedagogika: Jurnal Ilmu-Ilmu Kependidikan Jurnal Biologi
Manalu, Kartika
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Physics Public Health Social Sciences Other

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Journal : Jurnal Biogenerasi

 1 
Hubungan Kekerabatan Kepiting Rajungan (Portunus sp.) Berdasarkan DNA Barcoding di Perairan Percut Sei Tuan Kabupaten Deli Serdang Zuhra, Arini; Manalu, Kartika; Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4834

Abstract

Rajungan crab (Portunus sp.) is one type of crab that has a fairly high commodity value, this species has a fairly wide distribution covering the Indo-Pacific Ocean and also offshore, this crab has an important role in ecological stability. Portunus sp. has a more attractive appearance compared to other crabs. The purpose of this study is to look at the (dominant) crab species found in January (west wind season) in the waters of Percut Sei Tuan based on the genes CO1, and phylogenetics (kinship relationship) of Portunus sp. in the waters of Percut with Portunus sp. in NCBI or Genebank. The method used in this study is DNA isolation on specimens using the Favorgen kit locus of the CO1 gene in the genome of the Portunus sp. amplified by PCR technique with LCO1940 (forward) and HCO2198 (reverse) primers. The results of the research that have been carried out have found 3 species from the genus Portunus, namely P. sanguinolentus, P. trituberculatus and P. pelagicus. Of the three species found in the Percut Sei Tuan Waters, P. trituberculatus and P. pelagicus have a closer kinship relationship compared to P. sanguinolentus, based on the percentage of AT content and GC content, the AT content is higher at 61.03% which means that the three species have primitive properties. The genetic distance between P. sanguinolentus and P. trituberculatus is closer (19.32) compared to P. pelagicus. Based on the phylogenetic tree reconstruction, P. trituberculatus and P. sanguinolentus are closer to P. pelagicus with the Neighbor-Joining method and the Kimura-2-Parameter calculation model. Thus, it can be concluded that DNA barcoding using the CO1 gene locus can be used as a method in identifying Portunus sp. molecularly and kinship relationships with other species.
ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN Dwi Pratiwi, Anggrainy; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4835

Abstract

Anadara sp. clams are very popular clams among the community. Anadara sp. also usually live in waters with muddy and sandy surfaces. DNA barcoding is a barcode sequence to identify unknown species. CO1 is a molecular marker commonly used in molecular research on animals. The purpose of this study was to determine the species of Anadara sp. clams found in Percut Sei Tuan Waters using gene markers, the diversity of Anadara sp. clams using CO1 Gene Markers, and the kinship of Anadara sp. clams found in Percut Sei Tuan Waters. This study has 4 stages, namely DNA isolation using the Favorgen kit, PCR amplification, electrophoresis and sequencing. The DNA sequencing results were analyzed using the MEGA 11 application. The results showed that of the three samples that had been analyzed using the CO1 gene marker obtained using BLAST and NCBI, namely the Anadara granosa, Anadara antiquata, and Anadara gubernaculum samples. With the calculation results of higher AT content (28.9%) and lower GC content (20.12%) which states that the species is more primitive. In the pairwise distance matrix, the samples of Anadara antiquata (Percut Sei Tuan) and Anadara granosa (Percut Sei Tuan) which are in the same clade with different sub clades have a genetic distance ranging from 111.45 to 213.77. The phylogenetic tree also shows that the three samples have a close kinship relationship.
Co-Authors Adisty, Yuda Agusna, Sri Anas, Nirwana Andini, Pinkhan Aliffia Sekar Arbaiyah, Seri Aulya, Milda Ayundari, Sella Bintang, Safliah Rahmayani Dwi Pratiwi, Anggrainy Fatimah Azzahra Fermani, Nuritia Fitri Amalia Ginting, Monika Habibunnisa, Habibunnisa Hasibuan, Ira Aqrabi Br Hasra, Barratun Nisa Herni, Zulfiana Holila, Nur Hutasuhut, Melfa Aisyah Jayanti, Ummi Jayanti, Ummi Nur Afinni Dwi Julianty, Dwita Khadijah, Nur Khairuna Khairuna Kinanti, Aulia Andin Kirana, Fira Maha, Destri Br Najah, Nurun Nasution, Rizki Amelia Nst, Rizki Amelia Pasaribu, Nurul Alda Putri Lubis, Febrianti Putri, Amelisa Rafika Annisa, Rafika Rahmadina, Rahmadina Rambe, Riris Nurkholidah Raselia, Citra Rasyidah Rasyidah Raudhatul Jannah Rohani Rohani Sartika, Dea Shaharani, Adella Simarmata, Faried Raja Sinaga, Sabariah Siregar, Abdul Hasym Mazhurin Siregar, Khoirul Bahria Siti Fatimah Sucahyo, Ahmad Imam Tazkiah, Citra Utami, Mutiara Alya Widya Ulfa, Syarifah Zahratul Idami Zelila, Umma Selma Zuhra, Arini