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All Journal HAYATI Journal of Biosciences Journal of Tropical Biodiversity and Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Canrea Journal: Food Technology, Nutritions, and Culinary Journal Berkala Penelitian Hayati Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Eko Agus Suyono
Faculty Of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Kampus UGM, Yogyakarta 55281, Indonesia; Center Of Excellence For Microalgae Biorefinery, Universitas Gadjah Mada, Sekip K1A, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Exploration of Lipid Profile and Wax Ester Content from Local Strain Euglena sp. IDN33 Cultivated in Mixotrophic Condition Combined with Molasses Supplementation Astiti, Adam; Erfianti, Tia; Maghfiroh, Khusnul Qonita; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.805-818

Abstract

The transition from fossil fuels to sustainable renewable energy is currently growing rapidly. Euglena sp. can be the source of biofuel. Molasses, in addition to Euglena sp., can increase the levels of paramylon and wax esters produced. Therefore, this study aims to analyze cell growth, lipid profile, and wax ester content of Euglena sp. cultivated under mixotroph conditions with molasses. Euglena was cultivated under mixotrophic conditions with the addition of various concentrations of molasses (0, 10, 15, and 20 g/L) until the final log phase. Then, measurements were made on cell density, levels of lipids, proteins, and paramylon to measure the growth and metabolites profile of Euglena sp., along with lipid profile and wax ester analysis using GC-MS. The results of the study showed that 10 g/L molasses increased Euglena growth (SGR 0.1736± 0.0213 mg/ml) and carbohydrate (0.426 mg/ml) but reduced lipid accumulation (2.29 mg/ml). This treatment provides the best result before control. Molasses does not increase carbohydrates and protein but increases paramylon accumulation. The GC-MS assay detected more lipid profiles and concentrations of each lipid type in non-molasses-treated Euglena sp. compared to molasses-treated. Euglena was cultivated on molasses, which was dominated by palmitic acid and myristyl myristate with the absence of PUFA-type fatty acids in the cells, while the untreated ones were dominated by stearic acid and myristyl myristate. Thus, 10 g/L supplementation of molasses is the most effective treatment compared to others.
The effect of lignosulfonate chemical stimulator to the metabolites of Euglena sp. Luthfiana, Dwi Hardianti; Karilanata, Khalid Erlangga; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Amelia, Ria; Erfianti, Tia; Larasati, Ersi; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.30804

Abstract

Euglena sp. contains several metabolites that play a role in various sectors, likely biofuels, food, feed, and pharmaceuticals. Multiple treatments are required to increase the content of Euglena sp.to support its use. In this study, a chemical stimulant in the form of lignosulfonate was added to Euglena sp. in order to increase its natural potency as measured by lipid, carbohydrate, protein, paramylon content, and biomass productivity. Lignosulfonate is a macromolecular polyelectrolyte and anionic surfactant that is unique and multifunctional. This lignosulfonate can act as an emulsifier, dispersing agent, binder, and stabilizer of lipids in Euglena sp. cells. In addition to the lignosulfonate chemical stimulator, it can affect the growth process of Euglena sp. so that it can produce good-quality cells. The method used was a Completely Randomized Factorial Design (CRD) with three treatments and three replications. The results obtained indicated that the addition of lignosulfonate was significantly changed the cell and protein density tests. Therefore, the optimal concentration of lignosulfonate can be used as an agent to increase the growth rate and metabolites of Euglena sp. significantly.Keywords:BiofuelEuglena sp.Growth rateslignosulfonateMetabolites
The effect of lignosulfonate chemical stimulator to the metabolites of Euglena sp. Luthfiana, Dwi Hardianti; Karilanata, Khalid Erlangga; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Amelia, Ria; Erfianti, Tia; Larasati, Ersi; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.30804

Abstract

Euglena sp. contains several metabolites that play a role in various sectors, likely biofuels, food, feed, and pharmaceuticals. Multiple treatments are required to increase the content of Euglena sp.to support its use. In this study, a chemical stimulant in the form of lignosulfonate was added to Euglena sp. in order to increase its natural potency as measured by lipid, carbohydrate, protein, paramylon content, and biomass productivity. Lignosulfonate is a macromolecular polyelectrolyte and anionic surfactant that is unique and multifunctional. This lignosulfonate can act as an emulsifier, dispersing agent, binder, and stabilizer of lipids in Euglena sp. cells. In addition to the lignosulfonate chemical stimulator, it can affect the growth process of Euglena sp. so that it can produce good-quality cells. The method used was a Completely Randomized Factorial Design (CRD) with three treatments and three replications. The results obtained indicated that the addition of lignosulfonate was significantly changed the cell and protein density tests. Therefore, the optimal concentration of lignosulfonate can be used as an agent to increase the growth rate and metabolites of Euglena sp. significantly.Keywords:BiofuelEuglena sp.Growth rateslignosulfonateMetabolites
Co-Authors Achmad Rodiansyah Ainul Fitria Mahmudah Amalia Rahmawati Amelia, Ria Ardipeni, Witia Arief Budiman Arief Budiman Asmi Citra Malina Astiti, Adam Baihaqi, Yahya BUDI SETIADI DARYONO Dwi Listyorini Dwi Umi Siswanti Eko Nurcahya Dewi Erfianti, Tia Hardianto, Muhammad Andhi Himawan Tri Bayu Murti Petrus Irma Rohmawati Jamaluddin Fitrah Alam Karilanata, Khalid Erlangga Kurnianto, Dedy Laksmindra Fitria Larasati, Ersi Lucia Tri Suwanti, Lucia Tri Lukita Purnamayati Luthfiana, Dwi Hardianti M Iqbal Maulana Ginting Maghfiroh, Khusnul Qonita Mastika Marisahani Ulfah Mochammad Donny Koerniawan Mochammad Yuwono Mufida, Na’imatul Mulyati Sarto Nugroho Dewayanto, Nugroho Nurafifah, Istini Putri, Renata Adaranyssa Egistha Rahadian Yudo Rohmawati, Uun Sadewo, Brilian Ryan Satriayudistira, Jody Ashrib Sitoresmi Prabaningtyas Slamet Widiyanto Solly Aryza Tri Wahyu Setyaningrum, Tri Wahyu Ulfah Juniarti Siregar