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All Journal Pharmaciana: Jurnal Kefarmasian Althea Medical Journal Global Medical and Health Communication The Indonesian Biomedical Journal JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Jurnal Pengabdian Pada Masyarakat Health Information : Jurnal Penelitian Jurnal Aisyah : Jurnal Ilmu Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Astrid Feinisa Khairani
Department Of Biomedical Sciences Faculty Of Medicine Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Changes in The Aging Cytoskeleton and How Metformin Plays a Role in Delaying Aging Dini Dini; Achadiyani Achadiyani; Astrid Feinisa Khairani
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 9, No 1 (2024): March 2024
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30604/jika.v9i1.2672

Abstract

The cytoskeleton plays an important role in forming the cell framework and dynamics. The part that plays a role in supporting the spatial and mechanical functions of the cell is located in the cytoskeleton. Therefore, strategies to maintain the integrity and dynamics of the cytoskeleton have potential as therapies for age-related disorders. One thing that can be used to delay aging is metformin. Through AMPK activation, it can influence the cytoskeleton; namely, it can remodel the dynamics of the cytoskeleton by influencing structural changes in the actin cytoskeleton, which in turn will reduce the permeability of the filtration barrier in diabetic conditions and can increase insulin sensitivity, especially in old age, thereby delaying aging. In this review, we review the current understanding of the role played by the cytoskeleton in aging and review the opportunities and challenges for transitioning basic research into intervention development. Methods: Several major electronic databases, including PubMed, Scopus, and Cohcrane were used to select articles between April 2013 and April 2023. From the 8 existing literature, we present the activity of metformin as an aging delay. Cytoskeleton can change with age so it can affect the dynamics and structure of the cell. Metformin shows significant benefits, especially in delaying aging, through activation of the AMPK pathway so that it can increase the activity of mitochondria in producing ATP, which can affect the structure of the cytoskeleton. With this article on changes in the cytoskeleton with aging, it is hoped that insight into future research directions can be achieved.
Metode Antropometri Untuk Menilai Status Gizi : Sebuah Studi Literatur Ratumanan, Samuel Permana; Achadiyani, Achadiyani; Khairani, Astrid Feinisa
Health Information : Jurnal Penelitian Content Digitized
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthropometry is a science that quantitatively measures body composition. Anthropometry plays an important role in the health sector as a reference for diagnosis and clinical interventions, especially regarding nutritional status. Along with the times, anthropometric measurements are not only limited to conventional measurements (height, body mass index / BMI, etc.), but involve the latest advances in equipment and technology. Three-dimensional (3D) imaging is known to have precision and reliability comparable to conventional measurements, while DEXA is known to be the standard for bone density assessment. The writing method in this article is a literature review where the articles taken will be screened for relevance by reading the title and abstract. The results of the analysis of this study indicate that the use of the latest methods such as 3D imaging and DEXA is known to have similar results when compared to conventional measurements. The conclusion of this study is that 3D imaging is known to be precise and reliable so that it can be used for anthropometric examinations, while the DEXA method has been recognized as the gold standard in assessing bone density.
Soyghurt Supernatant on Mouse Embryonic Fibroblast (MEF) Cell Lantika, Uci Ary; Khairani, Astrid Feinisa
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.215 KB) | DOI: 10.29313/gmhc.v7i1.3245

Abstract

Yogurt is a functional food developed with various modifications in the fermentation process. Replacing animal milk into soymilk as raw material is one approach. Yogurt has a good effect on human health. Probiotic and bioactive compounds in yogurt can inhibit cell proliferation and stimulate apoptosis on the cancer cell line. However, there is no report about the effect of yogurt on a normal cell. This research was conducted to examine the impact of soyghurt supernatant intervention toward the viability of mouse embryonic fibroblast (MEF) cell. The is an in vitro study using MEF cell isolated from 10th days gestational age mice embryo conducted at Microbiology Laboratorium and Cell Culture and Cytogenetic Laboratory, Universitas Padjadjaran, Bandung in November 2018–January 2019. Soyghurt made from soymilk fermented by Lactobacillus bulgaricus ATCC 11842. The number of the bacterial colony calculated by total plate count (TPC) method and pH calculated by pH meter. Soyghurt supernatant was made from soyghurt and then intervened into MEF cells by 1–20% concentration. The cell viability showed in the 50% inhibitory concentration (IC50) analysis. The intervention of soyghurt supernatant at 1–20% concentration showed there was no proliferation inhibition until 50% population (IC50). However, from the morphology analysis, there was MEF cell morphology alteration on the group given soyghurt supernatant with >12.5% concentration. Counter mechanism effect from soymilk fermentation by probiotic could be the driver for this result. In conclusion, soyghurt supernatant intervention at 1–20% concentration did not have a cytotoxic effect on MEF cell, but enhancement of soyghurt supernatant concentration can increase cytotoxic potential. SUPERNATAN SOYGHURT PADA SEL MOUSE EMBRYONIC FIBROBLAST (MEF)Yoghurt merupakan functional food yang dikembangkan dengan berbagai modifikasi dalam proses pembuatannya. Mengganti susu hewan dengan susu kedelai sebagai bahan baku adalah salah satunya. Yoghurt memiliki efek yang baik bagi kesehatan manusia. Senyawa probiotik dan bioaktif pada yoghurt dapat menginhibisi proliferasi sel dan menstimulasi apoptosis pada sel lini kanker. Akan tetapi, tidak terdapat laporan mengenai efek yoghurt pada sel normal. Penelitian ini dilakukan untuk menguji pengaruh intervensi supernatan soyghurt terhadap viabilitas sel mouse embryonic fibroblast (MEF). Ini adalah penelitian in vitro menggunakan sel MEF yang diisolasi dari embrio tikus hari ke-10 usia kebuntingan yang dilakukan di Laboratorium Mikrobiologi dan Laboratorium Kultur Sel dan Sitogenetika, Universitas Padjadjaran, Bandung pada November 2018–Januari 2019. Soyghurt dibuat dari susu kedelai yang difermentasi oleh Lactobacillus bulgaricus ATCC 11842. Jumlah koloni bakteri dihitung dengan metode total plate count (TPC) dan pH diukur dengan pH meter. Supernatan soyghurt dibuat dari soyghurt dan kemudian diintervensi ke dalam sel MEF dengan konsentrasi 1–20%. Viabilitas sel ditunjukkan dalam analisis penghambatan 50% (IC50). Pemberian supernatan soyghurt konsentrasi 1–20% menunjukkan tidak terdapat inhibisi proliferasi 50% (IC50). Namun, dari analisis morfologi, terdapat perubahan morfologi sel MEF pada kelompok yang diberi supernatan soyghurt dengan konsentrasi >12,5%. Efek mekanisme yang saling meniadakan dari fermentasi susu kedelai dengan probiotik diduga menjadi mekanisme hasil dari penelitian ini. Simpulan, intervensi supernatan soyghurt pada konsentrasi 1–20% tidak memiliki efek sitotoksik pada sel MEF, namun peningkatan konsentrasi supernatan soyghurt dapat meningkatkan potensi sitotoksik.
Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay Alfarafisa, Nayla Majeda; Khairani, Astrid Feinisa; Achadiyani, Achadiyani; Chou, Yoan; Aulia, Fitri; Firdaus, Muhammad Bintang
Althea Medical Journal Vol 12, No 2 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v12n2.3954

Abstract

Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research.Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay.Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness.Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications.
Co-Authors Achadiyani Achadiyani Achadiyani Alfarafisa, Nayla Majeda Anjani, Gebby Reviana Apriliani, Ardhia Rizki Arima Kurnia Sari Dewi Nurcahyani Atika Hana Ilyasa Bashari, Muhammad Hasan Chou, Yoan Cynthia Noviyanti Defi Ardia Pramesti Dika Widia Nur Azizah Dimas Erlangga Luftimas Dimas Erlangga Luftimas Dimpuulina Erna Mariati Dini Dini Dwi Andini Enny Rohmawaty Febriana Kurniasari Firdaus, Muhammad Bintang Fitri Aulia Fitrianti Darusman Hanna Goenawan Hanna Goenawan Hermin Aminah Usman Irawati Irfani Jose Arimathea Julia Windi Gunadi Khafifah, Dinda Salsa Kuheinderan Radha Krishnan Labibah, Salma Nur Lala Yuliani Lantika, Uci Ary Laraswita, Sarah Meinarni, Retno Nadiah, Zahra Nadifah Diana Zalia Naura Mardhiyah Nugraha, Marwan Agung Nur Atik Nurhasanah Nurhasanah Putri Halleyana Putri Halleyana Adrikni Rahman Rahman, Putri Halleyana A Ramadanti, Sarah Nurul Ramdhani, Muhammad Farid Rani Septriana Rani Septrina Ratumanan, Samuel Permana Resti Santika Rika Mustika Risqi Dwi Putra Ronny Lesmana Sarah Nurul Ramadanti Setiawan Silvana Anggreini Rosa Siti Aminah Sobana Sudigdo Adi Sunarjati Sudigdo Adi Sunarjati Sudigdoadi Titing Nurhayati Uci Ary Lantika Umar Islami Unang Supratman Vita Murniati Tarawan Widad Aghnia Shalannandia Widad Aghnia Shalannandia