Claim Missing Document
Check
Articles

Found 34 Documents
Search

Analysis of Images of Neutrophil Lymphocyte Ratio and Bacteria Density Level of Helicobacter Pylori in Tissue in Gastritis Patients Fadia Rakhmalia; Anik Handayati; Evy Diah Woelansari; Mohd Nazil Salleh
International Journal of Advanced Health Science and Technology Vol. 2 No. 6 (2022): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v2i6.181

Abstract

Gastritis is an inflammatory disease that can last for a long time. Helicobacter pylori infection is known as the main cause of peptic ulcer and the most common cause of gastritis. Helicobacter Pylori infection will cause mononuclear (MN) and polymorphonuclear (PMN) cell infiltration and will increase the production of proinflammatory cytokines in the gastric mucosa compared to non-Helicobacter Pylori gastritis. Helicobacter Pylori with histopathological examination diagnosis. This type of research is descriptive observational with purposive sampling data collection technique at the Anatomical Pathology Laboratory Installation, RSPAL dr. Ramelan Surabaya. The data used are the results of a biopsy of 60 samples that were determined as gastritis with histopathological examination of the identification of Helicobacter Pylori bacteria in gastric tissue classified by the level of bacterial density using The Updated Sydney System, as well as haemogram data for complete blood examination to further calculate the neutrophil lymphocyte ratio (NLR). The data were processed using descriptive statistical tests to determine the average neutrophil lymphocyte ratio (NLR). Based on the statistics of the r-Spearman test, the density of Helicobacter Pylori, (Pν = 0.000) below < 0.005 indicates that there is a strong relationship between the neutrophil lymphocyte ratio (NLR) and the density of Helicobacter Pylori bacteria.
Design of Home Made Lyophilized Serum as a Control Material for Internal and External Quality Control in Clinical Laboratories Anik Handayati
Health Notions Vol 6, No 12 (2022): December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn61203

Abstract

Important control materials are used to achieve laboratory test quality. Control materials can be obtained from commercial serum or can be made independently in the form of pooled serum. Control materials commonly used in clinical laboratories are commercial control materials in lyophilized form. However, the price is quite expensive, so it is less efficient to use in laboratories where the average number of examinations is small. Homemade lyophilized control agents can be used as an alternative to expensive factory controls. This study aims to analyze the stability of homemade lyophilized serum as a control material for the parameters of glucose, uric acid, cholesterol, triglycerides, BUN, creatinine, SGOT and SGPT stored for 7 months. This research was an experimental study with a time series research design conducted at the Clinical Chemistry Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, in January 2022 to September 2022. Blood serum was processed using the freeze dry technique to obtain a lyophilized form. Lyophilized serum was stored at 2-8°C in the freezer, dissolved using aquabides and checked every month, for 7 months. The results of glucose, uric acid, SGOT, SGPT, creatinine, cholesterol, and triglyceride tests did not cross the CV line (CCV). The Levey Jennings graph shows that the results of glucose, uric acid, SGOT, SGPT, creatinine, BUN, cholesterol, and triglycerides were in the mean ± 2 SD and did not follow Westgard's law restrictions, meaning that the results of homemade lyophilized serum were well controlled (in control) for 7 months of storage. It can be concluded that the homemade lyophilized serum is stable over 7 months of storage. 
STABILITAS SERUM LYOPHILIZED HOMEMADE REKONSTITUSI TERHADAP KADAR KREATININ DAN BLOOD UREA NITROGEN (BUN) YANG DISIMPAN DALAM FREEZER Anggie Banowati Wibiseno; Anik Handayati; Syamsul Arifin
JURNAL MEDIA KESEHATAN Vol 15 No 2 (2022): Jurnal Media Kesehatan Poltekkes Kemenkes Bengkulu Volume 15 No 2 Desember 2022
Publisher : Politeknik Kesehatan Kemenkes Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33088/jmk.v15i2.837

Abstract

Quality control is an activity that can improve the quality of the laboratory which is carried out byconducting an examination using a control serum. Homemade lyophilized serum is an alternative control serumthat is relatively inexpensive and easy to obtain than commercial control serum. This study aimed to determinethe stability of reconstituted homemade lyophilized control serum stored for 8 weeks at a temperature of (-2)ºCto(-4)ºCand(-20)ºConcreatinineandBUNlevels. This research was conducted at the Clinical Laboratory of Poltekkes Kemenkes Surabaya in March-May 2022 with the type of experimental research and a one group pretest-posttest design. The reconstitutedhomemade lyophilized serum was stored at (-2)ºC to (-4)ºC and (-20)ºC, the serum was checked once a week for8weeks. The results of homemade lyophilized serum creatinine and BUN levels after reconstitution stored at (-2)ºC to (-4)ºC and (-20)ºC were not out of the control limit ±2SD so they could be declared stable with a CVcreatinine value 4-6% and BUN 7-8%. Based on the regression test analysis, it is known that storage time has aneffect of 51.3% on serumcreatinine levels stored at (-2)ºC to (-4)ºC and has an effect of 79.3% on serumcreatinine levels stored at (-20)ºC. Storage time has an effect of 76% on serum BUN levels stored at (-2)ºC to (-4)ºCand75.1%effectonserumcreatininelevels storedat(-20)ºC.
Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks Kadek Profit Hartani; Anik Handayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3278

Abstract

Improving health laboratory services is closely related to strengthening the quality of health laboratories. Serum control is part of the quality assurance of health laboratories. Lyophilized homemade serum is a type of homemade control serum. This research aims to determine the stability of lyophilized homemade control serum against Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels. This research used an experimental method with pooled sera as research material stored at -2°C to -4°C and -20°C for 8 weeks. Pooled sera were obtained from respondents who had no history of disease, were free from HIV, AIDS, and HBsAg. The research was conducted at the Reference Laboratory and Clinical Chemistry Laboratory Politeknik Kesehatan (Poltekkes) Kementerian Kesehatan, Surabaya. The results of the study were data analysis using linear regression test. The SGOT parameter at freezer temperature (-2 to -4°C) showed that the storage time had an effect of 90.97% with an error component of 9.03%. At freezer temperature (-20°C), the result of storage time has an effect of 78.71% with an error component of 21.29%. In the SGPT parameter with freezer temperature (-2 to -4°C), the result of storage time has an effect of 96.71% with an error component of 3.29%. At freezer temperature (-20°C), the result of storage time has an effect of 91.47% with an error component of 8.53%. The results of the SGOT and SGPT examinations did not exceed the limits of ±2SD and ±3SD The CV on the SGOT and SGPT examinations also did not exceed the CCV limits, which is to be stable.
Stabilitas Serum Kontrol Liofilisat Buatan Sendiri Setelah Rekonstitusi Terhadap Kadar Kolesterol dan Trigliserida yang Disimpan dalam Freezer Suhu (-2°C) sampai (-4°C) dan (-20°C) Nada Nabilah Wulandari; Anik Handayati; Lully Hanni Endarini
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 14, No 1 (2023): Januari 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14114

Abstract

Lyophilisate control serum is a freeze-dried control serum. Control serum must be stable and can be examined for a long time in order to assess the performance of a laboratory, including the quality of tools and reagents. Cholesterol is a fat produced by the liver that causes heart disease in excess levels. Triglycerides are a form of fat storage in the body which functions as a source of energy. This study aimed to determine the stability of homemade lyophilisate control serum after reconstitution on cholesterol and triglyceride levels stored in the freezer at (-2°C) to (-4°C) and (-20°C). This study applied a one group post-test design. Samples were examined for 8 weeks with venous blood test material for lyophilisate control serum. The results of the analysis showed that cholesterol levels with storage for 8 weeks at temperatures (-2°C) to (-4°C) and (-20°C) were stable with an average within the range of ±2SD and CV between 1-6 %. Triglyceride levels with storage for 8 weeks at (-2°C) to (-4°C) were stable with an average within the range of ±2SD and a CV between 2-7%. R-square on the storage time of 8 weeks of lyophilisate control sera after reconstitution showed that storage time had a 16.35% effect on cholesterol stability at temperature (-2°C) to (-4°C), 0% on cholesterol stability at temperature (-20°C) ), 41.7% on triglyceride stability at temperature (-2°C) to (-4°C), and 32.52% on triglyceride stability at temperature (-20°C). The conclusion of this study is that the control sera of homemade lyophilisate after reconstitution with cholesterol and triglyceride parameters stored for 8 weeks is still stable and has a standard deviation within limits.Keywords: lyophilisate serum; reconstitution; cholesterol; triglycerides ABSTRAK Serum kontrol liofilisat merupakan serum kontrol bentuk beku kering. Serum kontrol harus stabil dan dapat diperiksa dalam jangka waktu yang lama agar dapat menilai kinerja suatu laboratorium, termasuk kualitas alat dan reagensia. Kolesterol merupakan lemak yang diproduksi oleh hati yang menyebabkan penyakit jantung dalam kadar berlebih. Trigliserida merupakan bentuk simpanan lemak dalam tubuh yang berfungsi sebagai sumber energi. Penelitian ini bertujuan untuk mengetahui stabilitas serum kontrol liofilisat buatan sendiri setelah rekonstitusi terhadap kadar kolesterol dan trigliserida yang disimpan dalam freezer suhu (-2°C) sampai (-4°C) dan (-20°C). Penelitian ini menerapkan one group post-test design. Sampel diperiksa selama 8 minggu dengan bahan uji darah vena untuk serum kontrol liofilisat. Hasil analisis menunjukkan bahwa kadar kolesterol dengan penyimpanan selama 8 minggu pada suhu (-2°C) sampai (-4°C) dan (-20°C) adalah stabil dengan rata-rata dalam batas rentang ±2SD dan CV antara 1-6%. Kadar trigliserida dengan penyimpanan selama 8 minggu pada suhu (-2°C) sampai (-4°C) adalah stabil dengan rata-rata dalam batas rentang ±2SD dan CV antara 2-7%. R-square pada lama penyimpanan 8 minggu serum kontrol liofilisat setelah rekonstitusi menunjukkan lama penyimpanan berpengaruh 16,35% terhadap stabilitas kolesterol suhu (-2°C) sampai (-4°C), 0% terhadap stabilitas kolesterol suhu (-20°C), 41,7% terhadap stabilitas trigliserida suhu (-2°C) sampai (-4°C), dan 32,52% terhadap stabilitas trigliserida suhu (-20°C). Kesimpulan dari penelitian ini adalah bahwa serum kontrol liofilisat buatan sendiri setelah rekonstitusi dengan parameter kolesterol dan trigliserida yang disimpan selama 8 minggu masih stabil dan mempunyai standar deviasi dalam batas.Kata kunci: serum liofilisat; rekontitusi; kolesterol; trigliserida
Uji Komparasi Hasil Pemeriksaan Hemoglobin Menggunakan Hematology Analyzer dan Hemoglobin Meter pada Pasien Kadar Normal dan Abnormal Rendah Fitria Yulfirda Arini; Anik Handayati; Sri Sulami Endah Astuti; Anita Dwi Anggraini
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 14, No 1 (2023): Januari 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14150

Abstract

Examination of hemoglobin is a hematological parameter that is often carried out to determine the level of health problems such as anemia or polycythemia using a hematology analyzer and hemoglobin meter. The purpose of this study was to determine the differences in the results of hemoglobin examination using a hematology analyzer and hemoglobin meter in patients with normal and low abnormal hemoglobin levels and to determine the accuracy and precision of the hematology analyzer and hemoglobin meter. The type of research used was observational with a cross-sectional design. This research was conducted at the Galis Health Center, Galis, Pamekasan Regency in March 2022. The research subjects were 50 people with normal hemoglobin levels (12-16 g/dL) and 50 people with low abnormal hemoglobin levels (<12 g/dL). Data were analyzed using the Wilcoxon test. The results of the analysis showed that the mean values of hemoglobin examination results using a hematology analyzer and hemoglobin meter in patients with normal Hb levels were 13.49 g/dL and 13.0 g/dL; whereas in patients with abnormally low Hb levels were 10.32 g/dL and 9.88 g/dL. The results of evaluating the accuracy and precision of normal control blood levels using a hematology analyzer were 101.99% and 0.89%, while using a hemoglobin meter were 96.38% and 1.06%. The results of evaluating the accuracy and precision of low-level control blood using a hematology analyzer were 103.17% and 2.18%, while using a hemoglobin meter were 96.83% and 2.32%. Wilcoxon test showed p = 0.000. It was concluded that there were differences in hemoglobin levels in patients with normal and abnormally low Hb levels between those using a hematology analyzer and a hemoglobin meter.Keywords: hemoglobin; hematology analyzers; hemoglobin meters ABSTRAK Pemeriksaan hemoglobin merupakan parameter hematologi yang sering dilakukan untuk mengetahui tingkat gangguan kesehatan seperti anemia atau polisetemia menggunakan alat hematology analyzer dan hemoglobin meter. Tujuan penelitian ini adalah untuk mengetahui perbedaan hasil pemeriksaan hemoglobin menggunakan alat hematology analyzer dan hemoglobin meter pada pasien kadar hemoglobin normal dan abnormal rendah serta untuk mengetahui akurasi dan presisi dari alat hematology analyzer dan hemoglobin meter. Jenis penelitian yang digunakan adalah observasional dengan rancangan cross-sectional. Penelitian ini dilakukan di Puskesmas Galis, Kecamatan Galis, Kabupaten Pamekasan pada bulan Maret 2022. Subjek penelitian yaitu 50 orang dengan kadar hemoglobin normal (12-16 g/dL) dan 50 orang dengan kadar hemoglobin abnormal rendah (<12 g/dL). Data dianalisis menggunakan yaitu uji Wilcoxon. Hasil analisis menunjukkan bahwa nilai rerata hasil pemeriksaan hemoglobin menggunakan hematology analyzer dan hemoglobin meter pada pasien dengan kadar Hb normal adalah 13,49 g/dL dan 13,0 g/dL; sedangkan pada pasien dengan kadar Hb abnormal rendah adalah 10,32 g/dL dan 9,88 g/dL. Hasil evaluasi akurasi dan presisi dari darah kontrol level normal menggunakan hematology analyzer adalah 101,99% dan 0,89%, sedangkan dengan hemoglobin meter adalah 96,38% dan 1,06%. Hasil evaluasi akurasi dan presisi dari darah kontrol level rendah menggunakan hematology analyzer adalah 103,17% dan 2,18%, sedangkan dengan hemoglobin meter adalah 96,83% dan 2,32%. Uji Wilcoxon menunjukka p = 0,000. Disimpulkan bahwa terdapat perbedaan kadar hemoglobin pada pasien dengan kadar Hb normal dan abnormal rendah antara yang menggunakan hematology analyzer dan hemoglobin meter.Kata kunci: hemoglobin; hematology analyzer; hemoglobin meter
PERBANDINGAN HASIL LAJU ENDAP DARAH METODE WESTERGREN DENGAN MENGGUNAKAN ANTIKOAGULAN EDTA DAN NATRIUM SITRAT 3,8% PADA WANITA MENSTRUASI Puji Lestari Atsania Putri; Handayati Anik; Endah Astuti Sri Sulami
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.883 KB)

Abstract

The Erythrocyte sedimentation rate is a routine blood test performed to monitor the course of the disease where high values ​​are likely to be associated with the presence of inflammation. ICSH (International Council for Standardization in Hematology) recommends the Westergren method as a method for examining LED with several developments in terms of the anticoagulants and diluents used. Anticoagulants that can be used are Sodium Citrate 3,8% anticoagulant and EDTA (Ethylene Diamine Tetra-Acetid-Acid) anticoagulant. This study aims to determine the differences in the erythrocyte sedimentation rate of the westergren method using EDTA anticoagulants and sodium citrate 3.8%.This study uses primary data collection techniques with comparative research type. The research was conducted at the Health Analyst Laboratory of Poltekkes Kemenkes Surabaya, in January-April 2021. The sample of the study was 50 menstruating women who had met several criteria, who would be examined for LED values ​​using the Westergren method using EDTA anticoagulants in purple vacuum tubes and sodium citrate 3,8 % on a black vacuum tube.The results showed that the mean of sedimentation rate with EDTA anticoagulant was 27.66 mm/hour and 22.04 mm/hour for sodium citrate 3,8% anticoagulant. The statistical test was carried out by using the paired sample t-test which showed the sig (p) value = 0.000 0.05, so it could be concluded that there was a difference between the EDTA anticoagulant LED and the sodium citrate anticoagulant LED. Thus the EDTA anticoagulant can still be used as an anticoagulant in examining the erythrocyte sedimentation rate
HUBUNGAN JUMLAH SEL NEUTROFIL DENGAN KADAR TROPONIN I PADA PENDERITA INFARK MIOKARD AKUT Candra Triana Hikma; Handayati Anik; Endah Astuti Sri Sulami
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656 KB)

Abstract

Acute Myocardial Infarction (AMI) is a heart disease condition due to heart muscle cells not getting enough blood flow and oxygen due to atherosclerosis of the coronary arteries. When the condition of atherosclerosis gets worse, it will cause an inflammatory reaction, causing an increase in neutrophils. Inflammation due to atherosclerosis will worsen blood flow to the heart, resulting in heart muscle necrosis which causes an increase in biochemical markers, namely cardiac troponin. The aim of this research is to determine the relationship between the number of neutrophil cells and troponin I levels in patients with Acute Myocardial Infarction.This type of research is a cross-sectional analytic observational with a retrospective approach. This research was conducted on patients with Acute Myocardial Infarction at the RSPAL dr. Ramelan Surabaya who performed a complete blood count and troponin I level with a period of January 1st 2020 – May 3rd 2021, taken by purposive sampling. The results showed that male respondents were 29 patients (63%) while female respondents were 17 patients (37%). Respondents aged 65 years amounted to 28 (61%) people, while respondents aged 65 years were 18 (39%) people. The results of the hypothesis test have a sig value of 0.000 < (0.05), so there is a relationship between the number of Neutrophil cells and Troponin I level in patients with Acute Myocardial Infarction. In hypothesis testing, the correlation coefficient value is 0.594, which means that there is a moderate relationship with a positive direction.
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
The Effectiveness of Moringa Leaves in Controlling Blood Sugar Levels in Patients With Type 2 Diabetes Mellitus in Prolanis, Sampang District, Madura Anik Handayati; Pestariati Pestariati; Suhariyadi Suhariyadi
Frontiers in Community Service and Empowerment Vol. 1 No. 2 (2022): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v1i2.6

Abstract

Diabetes mellitus (DM) is a disease that leads to an increase in blood glucose levels, which in turn can lead to chronic diseases. The potential group to be socialized is the Prolanis group at Sreseh Health Centre, Sampang Madura. Moringa is a tree that grows very commonly in the Madura region, including in Sreseh sub-district. Moringa contains active ingredients that can lower blood glucose levels. People do not avail of the benefits of Moringa leaves  (Moringa aleivera), which are widely available in the region. The purpose of this community service activity is so that the community can be more optimal in controlling blood sugar levels, especially the use of Moringa leaf extract in lowering blood sugar levels. The method of conducting the activities is a group-based method that is carried out comprehensively, namely conducting regular and continuous counselling and consultations for Prolanis members. The activities of the community service teams are carried out in a measurable way and a monitoring and evaluation process is carried out to measure the achievement of the objectives and results. The results of the PKM activities have been identified: Community service activities (PKM) that have been well implemented, starting with survey and coordination activities, initial assessment activities, PKM implementation activities, and evaluation of the results achieved. The community, in this case, the Prolanis members with DM type 2, participated with great enthusiasm in each meeting/phase of the activities. The evaluation of the results of the activities showed good results: The number of subjects with type 2 diabetes whose blood glucose levels were uncontrolled decreased by 61.54%.