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Evaluation of the Quality of Commercial Control Materials and Homemade Lyophilized on Clinical Chemistry Parameters with the Sigma Metric Method Maulida Azmi Wimasni Inah; Masdiva Putri Hidayah; Anik Handayati; Museyaroh; Lully Hanni Endarini; Edy Haryanto
Jurnal Teknokes Vol. 18 No. 3 (2025): September
Publisher : Jurusan Teknik Elektromedik, Politeknik Kesehatan Kemenkes Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Control materials are used to maintain the accuracy of testing procedures and the quality of laboratory results. However, commercial control materials are often considered less economical, so an alternative is needed, namely lyophilized human serum. Sigma metric helps improve laboratory operational costs efficiency through control settings and Westgard rule recommendations involving the Total Error Allowable (TEa) value, bias value (d%), and coefficient of variation (CV%). This study aims to determine the quality of commercial control materials and homemade lyophilized materials for blood glucose, uric acid, total cholesterol, and triglyceride parameters based on sigma values. This study used a comparative cross-sectional design. The samples included commercial control materials from one of the primary-level laboratories in Bangkalan District and homemade lyophilized samples from human serum collections. Data Analysis was conducted by statistically comparing the bias value and descriptively comparing the sigma value of commercial control material and homemade lyophilized. The results showed no significant difference in bias value between commercial control material and homemade lyophilized material for all parameters (significance value > α 0,05). The sigma values of commercial control material for blood glucose, uric acid, total cholesterol, and triglyceride parameters were 2.81 (poor), 2.41 (poor), 3.10 (marginal), and 2.14 (poor). The sigma values of the homemade lyophilized were 2.12 (poor), 1.76 (unacceptable), 3.36 (marginal), and 2.08 (poor). Based on the sigma values, the homemade lyophilized material was better for total cholesterol parameters, while the commercial control material was better for blood glucose, uric acid, and triglyceride parameters. It can be concluded that homemade lyophilized can be used as an alternative to commercial control materials, especially for total cholesterol parameters. These findings support the cost efficiency of laboratory operations through the development of more economical homemade lyophilized products that are suitable for use in laboratories.
Efforts to Control Blood Pressure for Prolanis Hypertension Members through the Utilization of Bay Leaf Decoction Handayati, Anik; Suhariyadi; Pestariati
Frontiers in Community Service and Empowerment Vol. 4 No. 3 (2025): September
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i3.104

Abstract

Bay leaves are a type of kitchen spice that is often added to dishes, inexpensive, and easily obtained. Research results have proven that the active compounds in bay leaves are quite effective and safer in lowering blood pressure. Most of the Sreseh residents work as fishermen who tend to consume salty foods, which causes a high incidence of hypertension. Therefore, it is necessary to increase knowledge about healthy lifestyles and the use of herbal medicine made from boiled bay leaves to lower blood pressure among Prolanis hypertension members in the Sreseh Health Center area. The methods used in this community service activity are lecture/presentation and demonstration methods. Lecture activities are carried out by delivering material on the prevention and natural treatment of hypertension using bay leaves, the benefits of bay leaves, and the processing of bay leaves for hypertension treatment. The demonstration method is carried out by showing how to process bay leaves. The target of the activity is Prolanis hypertension patients in Noreh Village, within the working area of the Sreseh Health Center, Sampang, Madura. Members of the Chronic Disease Management Program (PROLANIS), especially those with hypertension, were very enthusiastic and motivated to participate in every stage of the community service activity, from blood pressure checks, Prolanis exercises, to education on the benefits of bay leaves. After consuming boiled bay leaves, blood pressure checks on Prolanis members with hypertension showed a 30% decrease in the number of Prolanis members with hypertension, from the previous 76.67% to 46.67%.
Variance Index Score (Vis) Evaluation Of Homemade And Commercial Control Sera On Ast And Alt Assays Rahmania, Adelia Prisma; Handayati, Anik; Museyaroh, Museyaroh; Haryanto, Edy
Jurnal Riset Kesehatan Vol 13 No 2 (2024): NOVEMBER 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v13i2.11778

Abstract

Control materials are used as indicators of monitoring the performance of laboratory examinations so that the results issued are valid. Method of assessing the implementation of External Quality Assurance (EQAS) used Variance Index Score (VIS). This study aims to determine difference between pooled sera, homemade and commercial lyophilizates on AST and ALT parameters based on VIS. This research used comparative descriptive with quantitative approach in December 2023-May 2024. Homemade lyophilizates performed at UBAYA technobiology laboratory, determination of true value performed at 2 reference laboratories and clinical chemistry laboratory Poltekkes Surabaya, and determination of VIS performed at 10 primary level clinical laboratories in Surabaya Raya which determined by purposive sampling. The statistical analysis results showed no difference between homemade and commercial control materials based on VIS to the true value and all participating laboratories, so it was concluded that homemade control materials could be used as a substitute in implementing EQAS.
Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks Hartani, Kadek Profit; Handayati, Anik
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3278

Abstract

Improving health laboratory services is closely related to strengthening the quality of health laboratories. Serum control is part of the quality assurance of health laboratories. Lyophilized homemade serum is a type of homemade control serum. This research aims to determine the stability of lyophilized homemade control serum against Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels. This research used an experimental method with pooled sera as research material stored at -2°C to -4°C and -20°C for 8 weeks. Pooled sera were obtained from respondents who had no history of disease, were free from HIV, AIDS, and HBsAg. The research was conducted at the Reference Laboratory and Clinical Chemistry Laboratory Politeknik Kesehatan (Poltekkes) Kementerian Kesehatan, Surabaya. The results of the study were data analysis using linear regression test. The SGOT parameter at freezer temperature (-2 to -4°C) showed that the storage time had an effect of 90.97% with an error component of 9.03%. At freezer temperature (-20°C), the result of storage time has an effect of 78.71% with an error component of 21.29%. In the SGPT parameter with freezer temperature (-2 to -4°C), the result of storage time has an effect of 96.71% with an error component of 3.29%. At freezer temperature (-20°C), the result of storage time has an effect of 91.47% with an error component of 8.53%. The results of the SGOT and SGPT examinations did not exceed the limits of ±2SD and ±3SD The CV on the SGOT and SGPT examinations also did not exceed the CCV limits, which is to be stable.