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All Journal Jurnal Kedokteran Syiah Kuala International Journal of Integrated Health Sciences Pharmaciana: Jurnal Kefarmasian Media Ilmu Keolahragaan Indonesia Buletin Farmatera Jurnal Biomedika dan Kesehatan Jurnal Keperawatan dan Fisioterapi (JKF) Jurnal Kesmas dan Gizi (JKG) IJOEP : International Journal of Ecophysiology Narra J
Yamamoto, Zulham
Department Of Histology, Faculty Of Medicine, Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Journal : Buletin Farmatera

 1 
Salivary Microflora and Its Diagnostic Importance Dina Rahmi Solihad Nasution; Zulham Yamamoto
Buletin Farmatera Vol 7, No 3 (2022)
Publisher : Universitas Muhammadiyah Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/bf.v7i3.12199

Abstract

Saliva as a source of DNA has several advantages over blood. These advantages include easier sample collection, the sampling process can be carried out independently by participants, and stability. The study of the normal microflora of human saliva is increasingly in demand because it is related to the oral health status of each individual. Several studies explain microflora associated with diabetes, cancer, rheumatic heart disease, and others. Other things that affect the composition of the microflora of each individual include living habits, hygiene, age, and smoking. The microflora in saliva are protozoa, bacteria, yeast, and viruses. In healthy people, the characteristics of normal oral microflora found are dominant Gram-positive cocci and rods such as Actinomycetes spp and Streptococcus spp. Protozoa commonly found in saliva are Entamoeba gingivalis and Trichomonas tenax, while from the yeast Candida albicans group, the viruses most commonly found in saliva are herpes and retrovirus. Identification of normal salivary microflora can be done through microbiological and biomolecular approaches. Identification of microflora using a biomolecular approach, currently the most commonly used markers are 16S rRNA (bacteria) and ITS rDNA (fungi).
DEOXYRIBONUCLEASE (DNAse) DAN INHIBITOR DNAse Rambe, Ismatul Fauziah; Yamamoto, Zulham
Buletin Farmatera Vol 7, No 1 (2022)
Publisher : Universitas Muhammadiyah Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/bf.v7i1.8984

Abstract

Deoxyribonuclease (DNAse) is an enzyme that plays an important role in cell apoptosis and has the function of hydrolyzing DNA. There are two types of DNAse, namely i) DNAse I which works well at neutral pH (6.5-8.0) and requires bivalent ions such as magnesium (Mg2+) and calcium (Ca2+) for activation, and ii) DNAse II which works by good at acidic pH and does not require bivalent ions. DNAse inhibitors are required to control the activity of DNAse. Both DNAse and its inhibitors have a role in the pathogenesis of various diseases, such as in the autoimmune disease systemic lupus erythematosus. DNase inhibitors can be obtained from natural, conventional, or chemical sources.
Co-Authors Amin Soebandrio Bambang Prayugo Bayu Dewanto Chatarina Umbul Wahyuni Evirosa Juliartha Simanjuntak Evita Mayasari Farhat Gembira Arkemo Situmorang Harahap, Desy Aryani Jelita Siregar Kevin Kevin Kusumawati, R. Lia M. Ichwan Muhammad Ichwan Muhammad Ichwan Novrina Situmorang Nuraiza Meutia Pandia, Pandiaman Rahmi Rahmi Rambe, Ismatul Fauziah Raudah Putri Syari Rina Yunita Ririe Fachrina Malisie Sembiring, Rosita J. Sinaga, Bintang YM. Sulviani, Nurul Syahfira A Syahna Wahyuni, Arlinda S. Widjaja, Sry Suryani Yetty Machrina