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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture BERITA BIOLOGI Jurnal Penelitian Pertanian Terapan Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal
Kustantinah Kustantinah
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Veterinary Other

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Journal : BERITA BIOLOGI

 1 
INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1729.405 KB) | DOI: 10.14203/bb.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
Co-Authors A. A. Sakti A. Agus A. Agus A. N. Kisworo Agung Purnomoadi Ali Agus Ali Agus Apriyani Nur Sariffudin Arif Nindyo Kisworo Arif Nur Wibowo B. Suhartanto B. Suwignyo Bambang Suhartanto Bambang Suwignyo Budi Prasetyo Widyobroto Cuk Tri Noviandi D. A. Priyadi D. Maharani Diahanvika Tri Sarvinda Djoko Soetrisno E. Dahono E. Indarto E.R. Orskov Edwin Indarto Endang Baliarti F. Holil H. Hartadi Hari Hartadi Hery Widijanto I G S. Budisatria, I G I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ismaya Ismaya Jamhari (Jamhari) Joko Daryatmo K Adhianto, K Kusuma Adhianto Kusuma Adhianto L. M. Yusiati Latifah Latifah Latifah, L. Lies Mira Yusiati M Socheh Maharani, D. Muhlisin Muhlisin N Ngadiyono, N N. D. Dono Nafiatul Umami Nimas Resmita Kawuriaji Nono Ngadiyono Novie Shintasari R. Utomo R. W. Nurcahyo Ratri Ratna Dewi Rendi Fathoni Hadi Retno Adiwiniarti, Retno Ristanto Utomo Rusman Rusman Setyono, W. Sigit Bintara Siti Muyasaroh Soemitro Padmowijoto Sudi Nurtini T. Hartatik T. Hartatik W. Nurcahyo Widya Kenshiana Putri Y. D. Artikarini Yusri Sapsuha Zulfa, I. H. Zuprizal (Zuprizal)