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All Journal Buletin Peternakan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Ternak Jurnal Sain Peternakan Indonesia JURNAL PETERNAKAN Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Medik Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Kumawula: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Halal Research Budimas : Jurnal Pengabdian Masyarakat Ovozoa: Journal of Animal Reproduction Journal of Tropical Animal Science and Technology Jurnal Peternakan Mahaputra Stock Peternakan Kutubkhanah Jurnal Penelitian Sosial Keagamaan Livestock and Animal Research Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Jurnal Inspirasi Peternakan IPTEKIN : Jurnal Kebijakan Pembangunan dan Inovasi Jurnal Agripet
Yendraliza -
Department Of Animal Science, Faculty Of Agriculture And Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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The Effectiveness of Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo (Bubalus bubalis): Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo Yendraliza Yendraliza; Allif Bukhori; Edi Erwan; Ria Sari Gail Sianturi; Diana Andrianita Kusumaningrum; Muhamad Rodiallah; Zumarni
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.17

Abstract

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets to determine the effectiveness of adding sucrose to tris-egg yolk diluent on the sperm quality of the swamp buffaloes produced by sexing with the egg albumin method. Semen was collected once a week for 12 consecutive weeks with an artificial vagina from three male buffaloes at the Indonesian Center for Animal Research and Development (ICARD) in April - June 2021. Semen was separated by sedimentation using 10% and 30% egg albumins. Semen from sexing was divided into four diluent treatments, namely 20% tris-egg yolk (TEY), TEY+0% sucrose, TEY+0.2% sucrose, TEY+0.3% sucrose, and TEY+0.4% sucrose. Parameters measured included: motility, viability, intact plasm membrane, and intact acrosome cap of buffalo sperm. The results showed the motility of X sperm: 43.75-47.5%, Y sperm: 40-45%, the viability of X sperm: 78.25-79.75% and Y sperm: 77.25-79.75%, intact plasm membrane of X sperm: 70.5-71.5% and Y sperm: 70.5-71%, and viable acrosome-intact for X sperm: 79-80% and Y sperm: 78.75-79.5%. The addition of sucrose up to 0.4% into tris-egg yolk diluent was proven can maintain motility, viability, and integrity of cell membrane and acrosome of spermatozoa of water buffalo that was sexed with egg albumin.
Co-Authors Ahmaddyan Sitorus Ali, Aryadi Allif Bukhori Amdes Amdes Anwar Efendi Harahap Anwar Efendi Harap Anwar Effendi Aprizal Aprizal Aprizal Aprizal Ariyanti, Ervina Arsyadi Ali B.P Zespin B.P. Zesfin Busro Busro, B. C. Arman Dewi Ananda Mucra Diana Andrianita Kusumaningrum Diana Andrianita Kusumaningrum, Diana Andrianita Edi Erwan Edi Erwan Eka Yuliana Elfawati Elfawati Elfawati Elfawati Elviriadi Elviriadi Febriyanti , Rahmi Fitra, Deni Gevisioner Guswanto Guswanto Heru Gunawan Husnul Abadi Irawati, Evi Irdha Mirdhayati Irdha Mirdhayati Irdon Irsyadi Siradjuddin Irwan Taslapratama Istiannah, Fadila Jaswandi . Jully Handoko July Handoko Khairuddin Kurniawan, Isal Larasati, Kania Lia Lia M Rodiallah M. Rodiallah Magfirah Magfirah Muahamad Rodiallah Muhamad Rodiallah Muhammad Arifsyah Muhammad Musyrifin Muhammad Ridho Muhammad Rodiallah Muhammad Rodiallah Muhammad Rodiallah Muhammad Rodiallah N. Darmagiri Oksana Oksana Priyandi, Ahmad R. Misrianti Rahmad Yusuf Rahmi Febriyanti Rahmi Febriyanti Restu Misrianti Ria Sari Gail Sianturi Rodiallah, Muhamad Romus, Mahendra Sadarman Samaruddin Siregar Sianturi, Ria Sari Gail Sugeng Santoso T. Adelina Tania Tania tania, tania Tri Astuti Umar Faruq Yudi Gusti Rahman Z Udin Z. Udin Zakia, Ismon Zumarni Zumarni Zumarni Zumarni Zumarni Zumarni Zumarni Zumarni Zumarni, Zumarni