Article Per Year (5 Year)
p-Index From 2021 - 2026
1.295
P-Index
This Author published in this journals
All Journal Bioeksperimen: Jurnal Penelitian Biologi BIOEDUSCIENCE Jurnal Pembelajaran dan Biologi Nukleus Community Empowerment Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi
Tyastuti, Erma Musbita
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Physics Public Health Social Sciences

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : BIOEDUSCIENCE

 1 
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Syarifah, Siti Nur; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
Co-Authors Adityaradja, Bagas Aurelia, Fanisha Febriyanti, Vika Guntur Nurcahyanto Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Ima Aryani Kun Harismah Maimun, Muhammad Halim Mandiri, Listy Hasti Okid Parama Astirin Putri Agustina Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Santhyami Santhyami Sari, Siti Kartika Siddiq, Yasir Sunarto Sunarto Syarifah, Siti Nur Titik Suryani Triastuti Rahayu Yasir Sidiq