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All Journal Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Prosiding Seminar Biologi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian STOMATOGNATIC- Jurnal Kedokteran Gigi Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia
Sismindari .
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Other

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AKTIVITAS KEMOPREVENSI EKSTRAK TEMU KUNCI (BOESENBERGIA PANDURATA) PADA KARSINOGENESIS KULIT MENCIT BALB/C TERINDUKSI RADIASI ULTRA VIOLET Listyawati, Shanti; Sismindari, Sismindari; Mubarika, Sofia; B. Murti, Yosi
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Temu kunci (B.pandurata) mengandung senyawa-senyawa yang berpotensi antikanker. Penelitian ini bertujuan untuk mengkaji efek kemoprevensi ekstrak etanolik rimpang temu kunci pada karsinogenesis kulit terinduksi ultra violet. Ekstraksi serbuk rimpang B. pandurata dilakukan dengan metode maserasi  menggunakan etanol. Hewan uji yang digunakan adalah mencit Balb/C betina umur 28 hari yang dicukur rambut punggungnya, dikelompokkan menjadi 5 kelompok perlakuan, yaitu kelompok kontrol dan empat  kelompok   perlakuan ekstrak etanolik temu kunci yang diberikan secara oral. Induksi karsinogenesis dengan paparan UV dosis 0,167 J/cm2/hari, sebanyak 60 paparan (5 kali paparan/minggu). Efek penghambatan karsinogenesis kulit  dipelajari pada tingkat insidensi dan multiplisitas kanker. Hasil uji menunjukkan bahwa ekstrak etanolik temu kunci mampu menurunkan angka insidensi dan multiplisitas kanker kulit pada mencit Balb/C terinduksi UV. Ekstrak tersebut berpotensi   dikembangkan sebagai agen kemoprevensi kanker.   Kata Kunci: Boesenbergia pandurata, kemoprevensi, karsinogenesis , ultra violet,
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Validasi Metode Real-Time Polymerase Chain Reaction untuk Deteksi DNA Babi (Sus Scrofa Domestica) dan Celeng (Sus Barbatus) pada Sosis Sapi Mariyani, Mariyani; Sismindari, Sismindari; Rumiyati, Rumiyati
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.369 KB) | DOI: 10.36418/syntax-literate.v6i8.3806

Abstract

Babi (Sus scrofa domestica) dan celeng (Sus barbatus) hewan satu spesies yang memiliki kemiripan sangat tinggi, perbedaan keduanya dilihat dari susunan basa DNA yang dimiliki. Metode analisis DNA antara babi dan celeng banyak yang belum berhasil membedakan kedua spesies tersebut seperti pada penelitian yang dilakukan Hikmah (2019) untuk membedakan babi dan celeng menggunakan primer Nk-ND1 dengan metode real time PCR namun berhasil mendapatkan perbedaan basa antara fragmen DNA babi dan celeng dari hasil sekuensing sehingga perbedaan urutan basa tersebut dijadikan sebagai primer spesifik babi untuk membedakan kedua spesies babi dan celeng dengan metode real time PCR. Lima spesies hewan yang digunakan yaitu babi, celeng, sapi, kambing dan ayam, primer babi forward 5’-GATGCCCTAAAACTATTCACC-3’ dari hasil sekuensing dan primer babi reverse yaitu 5’-TAGTGCTAGGGATAAGGCTAGG-3’ desain Hikmah (2019), SsoFast™ EvaGreen® Supermix (Bio-Rad), proteinase-K (Invitrogen), isolasi DNA menggunakan Geneaid Genomic DNA Mini Kit. Validasi metode real time PCR dengan uji spesifitas primer terhadap 5 isolat DNA jaringan segar, uji efisiensi dan sensitivitas pada 6 konsentrasi DNA (50; 5; 0,5; 0,05; 0,005 dan 0,0005 ng) dan sosis referensi campuran daging sapi:babi (100%; 50%; 25%; 10%; 5% dan 1%), uji keterulangan dilakukan sebanyak 5 kali replikasi. Hasil analisis menunjukkan sepasang primer spesifik terhadap DNA babi dan celeng. Efisiensi DNA babi yaitu 109,5% dan R2 = 0,967, efisiensi celeng 99,1% dan R2 = 0,999. Batas deteksi absolut DNA babi dan celeng yaitu 0,05 ng/µL dan 0,5 ng/µL, batas deteksi relatif sosis babi yaitu 5% dan sosis celeng 1%. Nilai RSD rata-rata pada analisis keterulangan DNA babi dan celeng yaitu 4,210% dan 3,611%. Pada sampel tidak terdteeksi adanya DNA babi dan celeng, secara kuantitatif metode ini tidak memenuhi persyaratan validasi.
Solubilization Inclusion Bodies from Synthetic Recombinant PGA Gene Expressed in E. coli BL21(DE3) by Denaturing and Non-denaturing Agents Purwanto, Purwanto; Sismindari, Sismindari; Purwantini, Indah; Rumiyati, Rumiyati; Rasyidah, Muthi'ah; Mulia, Muhammad Adi
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 11 No. 3 (2024): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v11i32024.325-334

Abstract

Background: With the rise in green chemistry, the synthesis of antibiotic compounds through enzymatic processes is a preferred option. Penicillin-G acylase (PGA) is an important enzyme for producing important antibiotics, such as penicillin and its derivatives. Therefore, studies on PGA have been conducted worldwide. In the penicillin biosynthetic pathway, PGA catalyzes the conversion of penicillin G into 6-amino penicillanic acid (6-APA), a precursor for the enzymatic synthesis of penicillin derivatives. Unfortunately, bacteria naturally produce PGA in small quantities. Objective: One strategy for producing this enzyme in large quantities is DNA recombination, which is expressed in Escherichia coli. The formation of inclusion bodies (IBs) is a common obstacle to protein overexpression in Escherichia coli. In this study, we discuss IBs solubilization methods for recombinant PGA derived from E. coli (rPGAEc) expressed in E. coli BL21 (DE3). Recombinant E. coli BL21 (DE3) cells harboring rPGAEc were induced with IPTG for enzyme expression. Induction was performed at 16 °C for 4 h and 24 h. The PGA enzyme expressed in the IBs form was then incubated in two solutions containing 8 M urea and 0.2% sarcosine to obtain a soluble enzyme. Results: Based on protein analysis by SDS-PAGE, a solution containing 8 M urea solubilized PGA more abundantly than 0,2% sarcosine. Conclusion: The solubilization technique of PGA expressed by E. coli proposed in this study is an alternative solution that can be considered for this purpose.
EFEK ANTI PROLIFERATIF EKSTRAK ETANOL KULIT BATANG TANAMAN CANGKRING (Erythrina fusca Lour) TERHADAP SEL MYELOMA Meiyanto, Edy; Sismindari, Sismindari; Triastuti, Asih
Jurnal Ilmiah Farmasi Vol. 1 No. 1 (2004)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTErythrina fusca Lour has been traditionally used to cure hepatosis, malaria, hematuria, andcancer. The bark of this plant contains  carotene, polifenol, thiamin, saponin, and alkaloiderythralin and erythramin. The aim of this research was to know the underlying mechanism of itseffect as antiproliferative against Myeloma cells. The bark powder was extracted using ethanol(70%) and was used for the experiment after freezed drying. Citotoxicity test of this extractperformed LC50 of 0,367 mg/ml. The rate of proliferation was observed by doubling time effectagainst proliferating cells. The cells were exposed with ethanolic extract in RPMI 1640 mediumcontaining 1) 0,25 mg/ml 2) 6,25x10-2mg/ml, and 3) 1,56x10-2mg/ml and every 0, 6, 12, 24, 48,and 72 hours cell were counted. The result showed that extract treated cells delayed proliferation atall concentration with doubling time dose 2) of 161, 38 hours, and dose 3) of 93,91 hours, whereasdoubling time of control cells were 69,86 hours. Ethidium bromide staining of extract treated cellsshowed apoptosis like profile. These results indicated that ethanolic extract of the bark of Erythrinafusca Lour has an antiproliverative effect on Myeloma cell line. Several mechanisms might accountfor this effect, like inhibiting cell cycle progression, signal transduction, causing delayed andapoptosisKeywords: Erythrina fusca Lour, atiproliferative, Myeloma
Co-Authors A. Suratman Achmad Fudholi Aditya K. Karim Aditya Krishar Karim Aminatun Munawarti, Aminatun Arifin, Muhammad Fajar Asih Triastuti Edy Meiyanto Endang Astuti Endang Semiarti Fortunella Tjondro Indah Purwantini Istriyati ., Istriyati J. O. Ughude Lina Winarti Mariyani Mulia, Muhammad Adi Ni Putu Linda Laksmiani Nining Sugihartini Puji Astuti Purwanto Purwanto Putu Riana Suastari Rahayu, Putu Riana Suastari Rasyidah, Muthi'ah Retno Sunarminingsih Sudibyo Ritmaleni, Ritmaleni Ronny Martien Rumiyati, Rumiyati Shanti Listyawati Sofia Mubarika Haryana Sudjadi ., Sudjadi Suwidjiyo Pramono Taryono, Taryono Tri Ratnaningsih Umar Anggara Jenie Widya Asmara Yosi B. Murti Zulies Ikawati, Zulies