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Journal : METAMORFOSA Journal of Biological Sciences

Potensi Jamur Endofit pada Tumbuhan Mangrove Avicennia marina di Kuala Enok Indragiri Hilir sebagai Penghasil Antibiotika Kustiasih Lestari; Anthoni Agustien; Akmal Djamaan
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p13

Abstract

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.
Pengaruh pH dan Suhu terhadap Produksi Antibiotika dari Isolat Bakteri Endofitik pada Tumbuhan Andalas (Morus macroura Miq.) Riski Budi Yani; Anthoni Agustien; Feskaharny Alamsjah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p14

Abstract

The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
Kemampuan Jamur Akar Putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam Mendegradasi Limbah Selulosa Dianty Putri; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p16

Abstract

Cellulose is one of abundance natural biopolymer with limited usage. The common found waste cellulose are waste straw, sawdust, and pineapple pit. Rigidoporus microporus is a white root fungi groups that often attack the roots of plants that rot and die. This study was conducted to assess the ability of white root fungi (R. microporus) to degrade waste cellulose, according to cellulolitic activity index of potential R. microporus isolate. The medium used was Carboxy Methyl Cellulose (CMC) with dye Congo Red (0,1%). The results obtained that R. microporus has cellulotic index 3,23 which indicated as potential cellulose degrade.
Antagonis Pseudomonas fluorescens indegenous terhadap Ralstonia solanacearum pada Tanaman Tomat (Lycopersicum esculentum) Armaleni Armaleni; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p19

Abstract

Tomato (L. esculentum) is one of the most important vegetable crops produce in West Sumatra. In this province, tomato are cultivated at highland regions of Solok, Padang Panjang, Tanah Datar and Bukittinggi. Annually, the increasing demand of this crop comes from other provinces in Sumatra and Java. This opportunity stimulate tomato’s agribusiness activity in West Sumatra. However, the crop succumb to the most destroyed bacterial wilt disease caused by Ralstonia solanacearum. The aims of this study to select antagonist Pseudomonas fluorescent as a biological control agent against R.solanacearum. The candidates of antagonist P. fluorescetn were collected from diseased tomato with R. solanacearum’s symptom in several tomato’s plantations in West Sumatra. Study of the antagonist was conducted from March to July 2016 at the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences Andalas University. To find the antagonist work, each of the collected candidate was grown opposite R. solanacearum on Nutrient Agar medium. The result found that there were16 isolates of P. fluorescent occurred. The best antagonist ability amongst the candidates was from Pfi with diameter zone was 4,95 cm.Keywords: tomato, Ralstonia solanacearum, Pseudomonas fluorescens, biological control agent, Pfi