Biodiesel is an environmentally friendly renewable fuel, but it has lower energy efficiency and can increase nitrogen oxide emissions. This study examines the effect of adding aluminum oxide nanoparticles (Al₂O₃) on the performance of diesel engines fueled with biodiesel. Five fuel combinations were tested: B30 and B30 with Al₂O₃ at concentrations of 30, 50, 70, and 90 ppm. The results show that the addition of Al₂O₃ increases the engine's torque and brake power, with the highest increases of 4.14% and 6.22% respectively in the B30+Al₂O₃ 70 ppm mixture under high load conditions. This effect is associated with better atomization, optimal air-fuel mixing, and the catalytic properties of the nanoparticles. However, at a concentration of 90 ppm, there is a slight decrease in performance due to nanoparticle aggregation. The optimal concentration of 70 ppm shows potential for improving biodiesel efficiency. Further studies are needed to evaluate the long-term stability and its impact on engine lifespan.