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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Prosiding Seminar Biologi The Journal of Experimental Life Sciences (JELS) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Jurnal Biodjati PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Proceeding Biology Education Conference ZOOTEC
Tri Ardyati
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Kimbal, Angie Via Resty; Jatmiko, Yoga Dwi; Ardyati, Tri
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

Abstract

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.
Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Hasanah, Uswatun; Ardyati, Tri; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Abstract

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.
Co-Authors AGUSTIN KRISNA WARDANI Anggraini, Ayu Arsyi Arie Srihardyastutie Baital Izzatul Badriyah Bambang Poerwadi Chintya Corin Citra Pradani P Dewi Permatasari, Dewi Dian Siswanto Dliyauddin, Moh Dwi Jatmiko, Yoga ekwan nofa wiratno Elok Zubaidah Faizah, Mamluatul Harmoko, Deni Hasria Alang Helena Daten Indah Nur Fitria Irfan Mustafa Ishak, Ismat Jamhari Jamhari Joni Kusnadi Jusup Subagja, Jusup Kimbal, Angie Via Resty LANGKAH SEMBIRING Lisa Lisdiana Luqman Qurata Aini Maulidiyah, Nuris Maulidiyah, Nuris Shobah Mora, Giral Baines Mulyawati, Alifia Issabella Munawaroh Munawaroh Osfar Sjofjan P Purwanto Pratiwi, Esti Rizkiana Purwanto, Agung Putra Ramadhan, Andi Muhammad Faiz Ramadhani, Sulistya Ika Ratna Fadhilah Israwan Restu Nugraha Sri Winarsih Suharjono, Suharjono SULISTIYANI SULISTIYANI Syamsul Arifin Uswatun Hasanah Wahyudi, Aleyda Nur Halizah Wulan Sari Eko Putri YOGA DWI JATMIKO