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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Journal of Food and Life Sciences Jurnal Teknologi Pertanian Proceeding International Conference on Global Resource Conservation AGRIVITA, Journal of Agricultural Science Indonesian Journal of Halal Research Biopendix: Jurnal Biologi, Pendidikan dan Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Medfarm: Jurnal Farmasi dan Kesehatan Indian Journal of Forensic Medicine & Toxicology Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science
Estri Laras Arumingtyas
Biology Study Program
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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GENETIC ANALYSIS AND MOLECULAR INTERACTION OF UGPase IN SOME TUBER PLANTS RELATED TO GLUCOMANNAN PRODUCTION Badiul Ulum; Estri Laras Arumingtyas; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : University of Brawijaya

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Abstract

UGPase is an enzyme that affect the conformation of polysaccharide with β 1-4 bond. There are some differences interaction of UGPase enzyme with UDP-Glucose substrate in some tuber plants. So, the aim of this research are to observe the interaction of UGPase and UDP-G in some tuber plants and to observe the relationship among tuber plant based on UGPase sequence. Result of molecular docking shows the catalytic domain interaction of UGPase and UDP-G in substrate binding loop, lower energy free obtain from A. konjac is -5.06 kcal/mol, following by I. batatas and S. tuberosum is -4,60 dan -3,73 kcal/mol respectively. Interaction was stabilize by some interaction type, there are electrostatic and hydrogen in A. konjac and I. batatas, and hydrophobic in S. tuberosum. Sequence alignment of UGPase shows I. batatas and S. tuberosum relatively close than to A. konjac.   Key words: interaction, UDP-Glucose, UGPase
Keragaman Morfologi Kenaf (Hibiscus cannabinus L.) KR 11 Mutan EMS (Ethyl Methanesulfonate) berdasarkan Panduan Karakterisasi Kenaf Rikza Hakin; Estri Laras Arumingtyas
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Abstract

Kenaf (Hibiscus cannabinus L.) merupakan salah satu sumber serat yang cukup potensial untuk dimanfaatkan secara optimal. Peningkatan pemanfaatan tanaman kenaf dapat dilakukan dengan menghasilkan varietas baru kenaf, salah satunya menggunakan EMS. Tujuan dari penelitian ini ialah mengetahui keragaman tanaman kenaf hasil mutasi EMS berdasarkan Panduan Karakterisasi Kenaf yang dikeluarkan oleh Departemen Pertanian. Penelitian dilakukan menggunakan 6 genotip berbeda, yaitu satu control (KR 11) dan lima genotip mutan EMS. Metode yang digunakan ialah melakukan pengamatan pada karakter morfologi yang meliputi karakter kualitatif dan kuantitatif dari karakter daun, bunga maupun karakter batang. Pengamatan karakter daun dilakukan pada saat tanaman berbunga pertama, karakter pembungaan diamati pada saat tanaman mengalami umur berbunga 50% serta karakter batang diamati pada saat panen. Hasil pengamatan kemudian disesuaikan dengan character state yang ditentukan dari Panduan Karakterisasi Kenaf dan dianalisis menggunakan software Clad97. Berdasarkan panduan tersebut, mutan yang memiliki keragaman paling rendah terhadap KR 11 ialah genotip mutan mutan 1 dengan nilai similaritas sebesar 94.4% sedangkan mutan dengan keragaman paling tinggi ditunjukkan oleh genotip mutan 4 dengan nilai similaritas sebesar 67.5%.
KERAGAMAN DAN KEKERABATAN KULTIVAR JAGUNG (Zea mays L.) LOKAL ASAL PULAU KISAR KABUPATEN MALUKU BERDASARKAN KARAKTER FENOTIP Hermalina Sinay; Estri Laras Arumingtyas; Nunung Harijati; Serafinah Indriyani
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page18-27

Abstract

Background: The relationship of plant kinship can be done through the identification of the phenotype character that is the character that appears on the individual or plant organism that can be both quantitative and qualitative. The purpose of this research is to know the diversity and kinship of corn cultivar from Kisar Island Regency of Maluku Southwest of Maluku Province based on phenotype character. Methods: The genetic material used was six local corn cultivars and one comparative variety. The six local corn cultivars are Red ruby with a brown cob, Blood red corn, Sticky, Ripe yellow, Deep yellow, and White, while the varieties are Srikandi varieties. To know the effect of cultivars and corn varieties on phenotypic diversity, variant analysis with Statistica Analytical System (SAS version 9.0) was used. To know the character that gives the greatest contribution to the diversity of cultivar phenotype and corn varieties, the main component analysis is done. To know the kinship among corn cultivars based on phenotypic character, gross analysis (cluster analysis) with hierarchy method is performed. The main component analysis and gameplay analysis was performed using SPSS version 18.0 computer program. Results: The results of variance analysis showed a significant effect of maize cultivar on phenotypic diversity. Characters that contribute to the diversity of the corn cultivar phenotype are the proline level, the weight of cobs without harvesting at harvest, the weight of cobs without dryness of the oven, the weight of the cobs at 12% moisture content, the unleaded cobs at 12%, the diameter of the ear, the number of seeds per ear, the number of rows of beans per ear, the number of leaves, and the length of the leaf. The results of Euclidean and Euclidean girder analysis show that the local yellow cultivars are in one group alone and have a low similarity with other local corn cultivars as well as the Srikandi-based varieties based on their phenotypic characters. Conclusion: There is a variety of phenotypes of local corn cultivars and varieties of Srikandi compounds grown on Kisar Island of Southwest Maluku Maluku Province.
Constructing Phenetic and Phylogenetic Relationship Using Clad'97 Brian Rahardi; Estri Laras Arumningtyas; Wayan Firdaus Mahmudi
Journal of Tropical Life Science Vol. 2 No. 1 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Relationship construction has a very important position in classification process for arranging taxonomy of organism. In the world of taxonomy, there are two the most familiar relationship diagram, cladogram and phenogram. In every construction activity, a researcher is always facing character state data from taxa that becomes components of the diagram. Calculation that is used for construction is often incorporate iterative or repetitive process that needs time and precision. The existence of calculating tools that produces both text and graphical output are hopefully decrease time and error during construction. Basic algorithm that is used in calculation is for phylogenetic construction by Kluge and Farris in 1969,for phenetic construction using cluster analysis with slight modification. Basic common algorithm used in the software is by calculating two dimensional arrays of taxa x characters matrix and creating distance or similarity matrix. In more detail the program creates one dimensional array of taxonomical object and each object has some other one dimensional array containing data commonly exist in a taxonomic unit. The relationship between one object and theother are regulated by an object that created by class representing taxonomic tree. Cladogram is constructed by calculating nearest distance between each taxon (OTU) and creating one HTU in every bifurcation. Phenogram is constructed agglomeratively by searching highest similarity between taxon then grouped into new taxon. Program calculates numerical data after we do character scoring. Final result for each user may be different; this may be due to decision by user during construction process. This paper hopefully attracts people from systematic computation to develop further into open source software and multi-platform feature.
Genetic Diversity Study Among Six Genera of Amaranth Family Found in Malang Based on RAPD Marker Arik Arubil Fatinah; Estri Laras Arumingtyas; Retno Mastuti
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Genera of amaranth family tend to have phenotypic variation partly caused by environmental factor. Phenotypic variation was the result of interaction between genetic and environmental factors. One of molecular markers that is widely used for detecting genetic variation is RAPD. RAPD is used for polymorphism detections and is now possible for identifiying a large number of loci and ascribes unambiguous taxonomic and genetic relationships among different taxa. Members of amaranth family found in Indonesia are Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, Gomphrena, Salsola, and Iresine. Six genera of which (Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, and Gomphrena) were observed in this study. DNA was extracted from fresh young leaves using Doyle and Doyles method with modification in the extraction buffer used. RAPD analyses were carried out with 20 decamer primers from Kit A of Operon Technology. DNA was amplified using master cycler gradient Eppendorf with 35 cycles. RAPD products were separated on 1,5 % agarose gels and detected by staining with ethidium bromide. There were 374 bands generated in 18 random primers. The number of monomorphic bands, polymorphic bands, and the percentage of polymorphism were 21 bands, 353 bands, and 94,38 % respectively. The high number and percentage of polymorphic bands revealed genomic DNA variation. This variation is in accordance with phenotypic variation detected in this experiment. Therefore, it can be concluded that, based on DNA polymorphism detected by RAPD, Amaranth family can be classified into two sub families namely Amaranthoideae and Gomphrenoideae.
Genetic variation of Dacrycarpus imbricatus in Bromo Tengger Semeru National Park, East Java Based on trnL (UAA) Intron Region Apriyono Rahadiantoro; Luchman Hakim; Estri Laras Arumingtyas
Journal of Tropical Life Science Vol. 3 No. 2 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The conservation of Jamuju Dacrycarpus imbricatus (Blume) de Laub. in Java Island has been considered important. One of the the limitation of such program is related to the viability data on the genetic diversity of species target. The aim of study was to determine genetic variation of D. imbricatus in Bromo Tengger Semeru Park, East Java based on trnL (UAA) intron region. DNA sample was collected from several D. imbricatus seedling population in Bromo Tengger Semeru National Park (BTSNP) in East Java. DNA was isolated and amplified using PCR. Genetic variation was estimated using trnL (UAA) intron sequences. This study confirm that D. imbricatus in Bromo Tengger Semeru has low genetic diversity. Based on the phylogenetic tree, D. imbricatus population from Bromo Tengger Semeru Park is closely related to D. imbricatus from Sabah-Malaysia and Hainan-China with 100 % similarity value. These data implies that population and habitat management of D. imbricatus in Bromo Tengger Semeru should be designed to enhance the population survival in the future.
Microsatellite Marker for Genetic Variation Analysis in Local Chili Pepper (Capsicum frutescens L.) Induced by Ethyl Methane Sulfonate (EMS) Ria Reinnata Juliandari; Retno Mastuti; Estri Laras Arumningtyas
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.08

Abstract

Mutation using Ethyl Methane Sulfonate (EMS) is a simple and quick method to produce genetic variation in chili pepper. In this study, a total of 3 genotypes of local chili pepper (Capsicum frutescens L.), i.e. Genotype 2 (G2), Genotype 7 (G7), and Genotype 11 (G11) were induced by EMS with concentrations of 0% (K0), 0.01% (K1), 0.02% (K2), and 0.04% (K3). Genetic variation analysis in mutant was performed based on 3 microsatellite markers CA 19, CA 27, CA 62. Those molecular markers successfully detected the genetic variation in chili pepper mutant based on the number and size of microsatellite alleles variation. The 3 genotypes of chili pepper mutant produced a total of 15 alleles with the average Polymorphism Information Content (PIC) value of 0.82. Compared to the control plant, genetic variations in genome level were observed in local chili pepper. Furthermore, the treatment of EMS with concentration of 0.04% produced the most notable genetic variation in 3 genotypes of local chili pepper. 
Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Chilli Pepper Cultivar: Physiology and Molecular Approach: Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Cultivar Arumingtyas, Estri Laras; Zakiyah, 'Ainun Sayyidah; Monica, Rosina; Siswanto, Dian
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.13

Abstract

The fruit color of chili pepper is an important characteristic in identification and classification and is often used as the basis for determining consumer preferences. Information on the relationship between chili fruit color and its molecular profile is very important in supporting selection activities in plant breeding. This study aims to identify genetic diversity associated with the fruit color of three genotypes of chili (Capsicum frutescens L.): Cakra Hijau, HV-149 and G1/M8, using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR). Morphological confirmation was carried out according to Capsicum descriptors. Nineteen RAPD markers and six SSR markers were used for genetic variability assessment. Genetic variation was analyzed using the unweighted pair group method with the arithmetic mean and the Jaccard similarity index. The three chili genotypes had different fruit colors at each maturation stage. The immature Cakra Hijau fruit is dark green and turns dark red as it ripens. The immature fruits of the G1/M8 line are light green and turn red when ripe. Finally, immature HV-149 fruits are dark green and yellow when ripe. The SSR markers used in this study were unable to show polymorphism. On the other hand, the RAPD marker successfully detected genetic variation in the three chili genotypes and resulted in a total of 49 alleles. The average value of polymorphic information content of the RAPD primers used ranged from 0 to 0.296, with the highest index indicated by OPA-1. The dendrogram shows the separation of the three genotypes into two main clusters, with the first cluster consisting of the HV-149 variety and the second cluster consisting of Cakra Hijau and G1/M8 lines. This study revealed that there are genetic variations based on the morphological characteristics of fruit color at each ripening stage and RAPD band profile. The RAPD marker was more effective than the SSR marker for identifying the genetic diversity of fruit color in the three chilies studied.
Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification Kusnadi, Joni; Hernandi, Kevin Hohn; Al-Awwaly, Khotibul Umam; Arumingtyas, Estri Laras; Hakiki, Hilda Maghfirotu; Istianah, Nur
Indonesian Journal of Halal Research Vol. 4 No. 2 (2022): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v4i2.15573

Abstract

Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species. A pair of species-specific primers encoding the CO1 gene were designed to amplify bovine DNA, tested for specificity, and applied in multiplex polymerase chain reaction (PCR) technique with D-loop primers for pigs, Cyt-b for rats, and 12S rRNA for dogs. The CO1 primers, along with D-loop primers for porcine, Cyt-b primers for rats, and 12S rRNA primers for dogs can be used to detect specific bovine DNA with a size of 279 bp and sequence similarity of 96%. In addition, dog, rat, and porcine DNA were amplified at 101, 603, and 951 bp, respectively. These four primers are specific and can amplify the target DNA to detect non-halal meat component contamination in a single reaction process.
Pengayaan Materi Nanoteknologi dan Integrasi Artificial Intelligence dalam Penelitian Biologi kepada Guru SMA Arumingtyas, Estri Laras
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 5, No 1 (2025)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.01.68

Abstract

Peningkatan kompetensi guru mutlak dilaksanakan untuk menjamin kualitas pendidikan. Pendidik harus mengikuti pengetahuan sesuai dengan perkembangan jaman dan teknologi terkini sehingga ilmu yang ditransfer kepada siswa adalah ilmu terkini. Nanoteknologi dan Artificial Intelligence (AI) adalah ilmu yang saat ini sedang berkembang pesat dan sangat diperlukan dalam pembelajaran. Tujuan kegiatan pengabdian kepada masyarakat yang dilaksanakan adalah untuk untuk meningkatkan pemahaman dan keterampilan guru tentang pemanfaatan AI dalam pembelajaran biologi dan pengetahuan tentang nanoteknologi bidang biologi. Metode pengabdian kepada masyarakat adalah Participatory Action Research yang melibatkan peserta secara aktif dalam kegiatan. Tahap kegiatan adalah identifikasi kebutuhan, perencanaan, pelaksanaan, dan evaluasi kegiatan. Peserta pelatihan sebanyak 22 orang guru SMA yang tergabung dalam Musyawarah Guru Mata Pelajaran Biologi Kota Malang. Materi yang disampaikan adalah materi nanoteknologi dan pemanfaatan AI dalam bidang biologi. Peserta mendapatkan materi AI tentang Chatbot yang dapat membantu dalam manajemen pembelajaran dan persiapan materi pembelajaran. Peserta melaksanakan praktik pemanfaatan AI menggunakan aplikasi Pl@ntNet. Pada akhir sesi pelatihan, peserta mendapatkan tugas untuk menguatkan pemahaman aplikasi AI dalam bidang biologi. Hasil pengabdian kepada masyarakat adalah terdapat peningkatan pengetahuan peserta tentang nanoteknologi dan AI sebesar 26% berdasarkan hasil kuesioner pre-test dan post-test. Peserta merasa sangat puas terhadap kinerja panitia dan fasilitas yang diberikan.
Co-Authors A.A. Ketut Agung Cahyawan W Andayani, Dian Wuri Andy Soegianto Andy Soegianto Anggraeni In Oktavia Apriyono Rahadiantoro Apriyono Rahadiantoro, Apriyono Arik Arubil Fatinah Azrianingsih , Rodiyati Badiul Ulum Badiul Ulum Brian Rahardi Catur Retnaningdyah Dedi Dedi Dian Siswanto Dr.Ir. Yunianta, DEA Dwinianti, Edia Fitri Edi Priyo Utomo Elok Zubaidah Evika Sandi Savitri Fatinah, Arik Arubil Filza Yulina Ade Hakiki, Hilda Maghfirotu Hermalina Sinay Hernandi, Kevin Hohn Ihwan Ihwan Joni Kusnadi Juliandari, Ria Reinnata Khotibul Umam Al-Awwaly Luchman Hakim Mahmudi, Wayan Firdaus Mochammad Roviq Mochammad Roviq Monica, Rosina Nensy Marantika, Riza Nia Kurniawan Noor Hidayat, Noor Nunung Harijati Nur Basuki Nur Basuki Nur Istianah Retno Mastuti Ria Reinnata Juliandari Rifa'i, Muhaimin Rikza Hakin Rodiyati Azrianingsih Rodiyati Azrianingsih Rosyidah, A’liyatur Sari, Dewi Ratih Tirto Serafinah Indriyani Siti Muslichah Sri Widyarti Sundari Sundari Tatik Wardiyati Tatik Wardiyati Wahyu Widoretno Wayan Firdaus Mahmudi Zakiyah, 'Ainun Sayyidah