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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Journal of Food and Life Sciences Jurnal Teknologi Pertanian Proceeding International Conference on Global Resource Conservation AGRIVITA, Journal of Agricultural Science Indonesian Journal of Halal Research Biopendix: Jurnal Biologi, Pendidikan dan Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Medfarm: Jurnal Farmasi dan Kesehatan Indian Journal of Forensic Medicine & Toxicology Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science
Estri Laras Arumingtyas
Biology Study Program
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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OPTIMIZATION OF GREEN SYNTHESIS OF GOLD NANOPARTICLES USING CHILI FRUIT EXTRACTS (Capsicum Sp.) Oktavia, Anggraeni In; Mastuti, Retno; Rosyidah, A’liyatur; Arumingtyas, Estri Laras
MEDFARM: Jurnal Farmasi dan Kesehatan Vol 14 No 1 (2025): Medfarm: Jurnal Farmasi dan Kesehatan
Publisher : LPPM Akafarma Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48191/medfarm.v14i1.502

Abstract

The use of plant extracts as reductants in green synthesis of gold metal nanoparticles (AuNPs) requires optimal conditions of temperature, extract concentration and pH. The objective of this research is to identify the optimal synthesis conditions for producing gold nanoparticles (AuNPs). The reducing agents employed are extracts from three varieties of chili peppers: Katokkon, Cakra Hijau, and Paprika Merah. Variations of plant extract concentrations of 10%, 15%, and 20%, incubation temperatures of 25°C, 37.5°C, and 50°C, and pH levels of 5, 7, and 9. The optimization formula design was determined using Box-Behnken, Design Expert version 13.0. The formation of AuNPs was indicated by a change in the color of the solution from yellow to pink to purple. Surface plasmon resonance was observed in the range of 200-800 nm using a microplate reader. Chili, Katokkon, Cakra Hijau and Red Paprika extracts were able to synthesize Au into AuNPs. The optimal formula for synthesizing AuNPs using Katokkon and Red Paprika extracts at a concentration of 10% while Ckra Hijau at a concentration of 15%. The incubation temperature used was 37.5°C for Katokkon extract and 25°C for Cakra Hijau and Red Pepper extract. The pH level used for the synthesis of AuNPs was pH 5 for all chili pepper extracts.
Genetic Diversity of Porang (Amorphophallus muelleri Blume) in Malang, East Java based on Morphology and Molecular Markers (ITS2, trnL, and matK): Genetic Diversity of Amorphophallus muelleri Blume in Malang Nensy Marantika, Riza; Arumingtyas, Estri Laras; Azrianingsih , Rodiyati
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.07

Abstract

Porang (Amorphophallus muelleri) is an herbaceous plant belonging to the Araceae family that is distributed in Java, including the Malang region. This study aimed to determine the morphological and genetic variation of porang in the Malang region based on ITS2, trnL, and matK molecular markers. Porang samples were collected from seven populations in Malang district, i.e., Kasembon, Ngantang, Pujon, Dampit, Kalipare, Pagak, and Bantur. Observa-tions of porang included 27 morphological traits, and young leaves were ob-tained for DNA extraction. Then, ITS2, trnL, and matK were amplified using a specific primer pair. Morphological traits exhibited similarity in color, surface, shape, and petiole pattern, while differences were apparent in morphological size parameters. Porang petiole of all the populations in Malang is green with a white prismatic pattern and linear lines. The 438 bp ITS2 sequences con-tained 390 bp invariable sites and 48 bp variable sites, whereas the 555 bp trnL intron sequences consisted of 511 bp invariable sites and 44 bp variable sites, and the 738 bp matK sequences comprised 713 bp invariable sites and 25 bp variable sites. The ITS2 sequences of the seven porang populations had the highest number of variable sites compared with the trnL and matK sequences. The total number of haplotypes based on ITS2, trnL, and matK was 11, with a haplotype diversity value of 1.00. Haplotype 1–7 were A. muelleri, haplotype 8 was A. paeoniifolius, and haplotypes 9, 10, and 11 were A. bulbifer, A. konjac, and A. sumawongii, respectively (from Genbank). Fur-thermore, phylogenetic analysis using Maximum Likelihood, Neighbor Joining, and Maximum Parsimony based on the ITS2, trnL, and matK sequences showed consistent clades. Clade 1 comprises A. muelleri, which is divided into 3 subclades. Subclade 1 comprises Kasembon, Ngantang, Pagak, Dampit, subclade 2 comprises Kalipare and Pujon, and subclade 3 is Bantur. Clade 2 is A. bulbifer, and clade 3 consists of outgroups.
Pengayaan Materi Nanoteknologi dan Integrasi Artificial Intelligence dalam Penelitian Biologi kepada Guru SMA Arumingtyas, Estri Laras
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 5 No 1 (2025)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.01.68

Abstract

Peningkatan kompetensi guru mutlak dilaksanakan untuk menjamin kualitas pendidikan. Pendidik harus mengikuti pengetahuan sesuai dengan perkembangan jaman dan teknologi terkini sehingga ilmu yang ditransfer kepada siswa adalah ilmu terkini. Nanoteknologi dan Artificial Intelligence (AI) adalah ilmu yang saat ini sedang berkembang pesat dan sangat diperlukan dalam pembelajaran. Tujuan kegiatan pengabdian kepada masyarakat yang dilaksanakan adalah untuk untuk meningkatkan pemahaman dan keterampilan guru tentang pemanfaatan AI dalam pembelajaran biologi dan pengetahuan tentang nanoteknologi bidang biologi. Metode pengabdian kepada masyarakat adalah Participatory Action Research yang melibatkan peserta secara aktif dalam kegiatan. Tahap kegiatan adalah identifikasi kebutuhan, perencanaan, pelaksanaan, dan evaluasi kegiatan. Peserta pelatihan sebanyak 22 orang guru SMA yang tergabung dalam Musyawarah Guru Mata Pelajaran Biologi Kota Malang. Materi yang disampaikan adalah materi nanoteknologi dan pemanfaatan AI dalam bidang biologi. Peserta mendapatkan materi AI tentang Chatbot yang dapat membantu dalam manajemen pembelajaran dan persiapan materi pembelajaran. Peserta melaksanakan praktik pemanfaatan AI menggunakan aplikasi Pl@ntNet. Pada akhir sesi pelatihan, peserta mendapatkan tugas untuk menguatkan pemahaman aplikasi AI dalam bidang biologi. Hasil pengabdian kepada masyarakat adalah terdapat peningkatan pengetahuan peserta tentang nanoteknologi dan AI sebesar 26% berdasarkan hasil kuesioner pre-test dan post-test. Peserta merasa sangat puas terhadap kinerja panitia dan fasilitas yang diberikan.
Employing SecA Recombinant Protein to Generate Polyclonal Antibodies for the Rapid Identification of Phytoplasma Fatinah, Arik Arubil; Rifa'i, Muhaimin; Arumingtyas, Estri Laras; Widyarti, Sri
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Phytoplasma, a plant pathogen that threatens plant health, can induce diseases such as yellow wilt, virescence, phyllody, and witches’ broom. Although early detection methods using polyclonal and monoclonal antibodies have been developed, synthetic peptides offer advantages such as higher titers, predictable antigenic characteristics and superior sensitivity and specificity. Epitope mapping plays a key role in designing peptide antigens and includes gene synthesis, transformation, expression assessment, large-scale production, and purification. A study using New Zealand male White rabbits was conducted to generate antibodies against phytoplasma. The SecA (395–470) sequence, obtained from the preprotein translocase meets the criteria of being non-homologous, nonallergic, and antigenic. The three-dimensional structure of SecA (395–470) has a sequential similarity of 77–97% with certain phytoplasma types, and the protein purity exceeds 90%. The production of polyclonal antibodies was successful, achieving titers greater than 1:512,000. The SWISS model was used to predict the 3D structure of SecA in phytoplasma species, revealing structural homology with other phytoplasma species. The recombinant protein antigen SecA was able to induce high-titer antibody formation (>1:512.000). The developed lateral flow immunoassay (LFA) detects phytoplasma in plants using purified and conjugated antibodies. The peptide design, derived from Aster yellows witches-broom (SrI), effectively detects phytoplasma from various groups, especially Cactus witches’ broom phytoplasma (SrII-C) and Peanut witches’ broom phytoplasma (SrII-A). The SrI group phytoplasma was chosen as a reference sequence due to its` ability to infect plants across the broadest host range. LFIA was tested on samples from both phytoplasma-infected and healthy plants using nested PCR. The SecA sequence was successfully produced and used as an immunogen candidate against phytoplasmas.
Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Chilli Pepper Cultivar: Physiology and Molecular Approach: Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Cultivar Arumingtyas, Estri Laras; Zakiyah, 'Ainun Sayyidah; Monica, Rosina; Siswanto, Dian
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.13

Abstract

The fruit color of chili pepper is an important characteristic in identification and classification and is often used as the basis for determining consumer preferences. Information on the relationship between chili fruit color and its molecular profile is very important in supporting selection activities in plant breeding. This study aims to identify genetic diversity associated with the fruit color of three genotypes of chili (Capsicum frutescens L.): Cakra Hijau, HV-149 and G1/M8, using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR). Morphological confirmation was carried out according to Capsicum descriptors. Nineteen RAPD markers and six SSR markers were used for genetic variability assessment. Genetic variation was analyzed using the unweighted pair group method with the arithmetic mean and the Jaccard similarity index. The three chili genotypes had different fruit colors at each maturation stage. The immature Cakra Hijau fruit is dark green and turns dark red as it ripens. The immature fruits of the G1/M8 line are light green and turn red when ripe. Finally, immature HV-149 fruits are dark green and yellow when ripe. The SSR markers used in this study were unable to show polymorphism. On the other hand, the RAPD marker successfully detected genetic variation in the three chili genotypes and resulted in a total of 49 alleles. The average value of polymorphic information content of the RAPD primers used ranged from 0 to 0.296, with the highest index indicated by OPA-1. The dendrogram shows the separation of the three genotypes into two main clusters, with the first cluster consisting of the HV-149 variety and the second cluster consisting of Cakra Hijau and G1/M8 lines. This study revealed that there are genetic variations based on the morphological characteristics of fruit color at each ripening stage and RAPD band profile. The RAPD marker was more effective than the SSR marker for identifying the genetic diversity of fruit color in the three chilies studied.
Co-Authors A.A. Ketut Agung Cahyawan W Andayani, Dian Wuri Andy Soegianto Andy Soegianto Anggraeni In Oktavia Apriyono Rahadiantoro Apriyono Rahadiantoro, Apriyono Arik Arubil Fatinah Azrianingsih , Rodiyati Badiul Ulum Badiul Ulum Brian Rahardi Catur Retnaningdyah Dedi Dedi Dian Siswanto Dr.Ir. Yunianta, DEA Dwinianti, Edia Fitri Edi Priyo Utomo Elok Zubaidah Evika Sandi Savitri Fatinah, Arik Arubil Filza Yulina Ade Hakiki, Hilda Maghfirotu Hermalina Sinay Hernandi, Kevin Hohn Ihwan Ihwan Joni Kusnadi Juliandari, Ria Reinnata Khotibul Umam Al-Awwaly Luchman Hakim Mahmudi, Wayan Firdaus Mochammad Roviq Mochammad Roviq Monica, Rosina Nensy Marantika, Riza Nia Kurniawan Noor Hidayat, Noor Nunung Harijati Nur Basuki Nur Basuki Nur Istianah Retno Mastuti Ria Reinnata Juliandari Rifa'i, Muhaimin Rikza Hakin Rodiyati Azrianingsih Rodiyati Azrianingsih Rosyidah, A’liyatur Sari, Dewi Ratih Tirto Serafinah Indriyani Siti Muslichah Sri Widyarti Sundari Sundari Tatik Wardiyati Tatik Wardiyati Wahyu Widoretno Wayan Firdaus Mahmudi Zakiyah, 'Ainun Sayyidah