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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Journal of Food and Life Sciences Jurnal Teknologi Pertanian Proceeding International Conference on Global Resource Conservation AGRIVITA, Journal of Agricultural Science Indonesian Journal of Halal Research Biopendix: Jurnal Biologi, Pendidikan dan Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Medfarm: Jurnal Farmasi dan Kesehatan Indian Journal of Forensic Medicine & Toxicology Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science

Estri Laras Arumingtyas

Biology Study Program

Author-ID : 234183

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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MICROSATELLITE MARKER FOR GENETIC VARIATION ANALYSIS IN LOCAL CHILI PEPPER (CAPSICUM FRUTESCENS L.) INDUCED BY ETHYL METHANE SULFONATE (EMS) Juliandari, Ria Reinnata; Mastuti, Retno; Arumningtyas, Estri Laras; Arumningtyas, Estri Laras
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Mutation using Ethyl Methane Sulfonate (EMS) is a simple and quick method to produce genetic variation in chili pepper. In this study, a total of 3 genotypes of local chili pepper (Capsicum frutescens L.), i.e. Genotype 2 (G2), Genotype 7 (G7), and Genotype 11 (G11) were induced by EMS with concentrations of 0% (K0), 0.01% (K1), 0.02% (K2), and 0.04% (K3). Genetic variation analysis in mutant was performed based on 3 microsatellite markers CA 19, CA 27, CA 62. Those molecular markers successfully detected the genetic variation in chili pepper mutant based on the number and size of microsatellite alleles variation. The 3 genotypes of chili pepper mutant produced a total of 15 alleles with the average Polymorphism Information Content (PIC) value of 0.82. Compared to the control plant, genetic variations in genome level were observed in local chili pepper. Furthermore, the treatment of EMS with concentration of 0.04% produced the most notable genetic variation in 3 genotypes of local chili pepper.

EKSTRAKSI SENYAWA BIOAKTIF CABAI RAWIT (CAPSICUM FRUTESCENS L.) MENGGUNAKAN METODE EKSTRAKSI GELOMBANG ULTRASONIK Kusnadi, Joni; Andayani, Dian Wuri; Zubaidah, Elok; Arumingtyas, Estri Laras
Jurnal Teknologi Pertanian Vol 20, No 2 (2019)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

ABSTRAK Cabai rawit (Capsicum frutenses L.) memiliki kandungan senyawa bioaktif seperti capsaicinoid, fenol, flavonoid dan vitamin C yang tinggi, sehingga berpotensi sebagai sumber antioksidan. Tujuan penelitian ini untuk mengetahui pengaruh rasio bahan:pelarut dan lama ekstraksi dengan gelombang ultrasonik/Ultrasonic Assisted Extraction (UAE) terhadap kandungan fitokimia dan aktivitas antioksidan dari ekstrak cabai rawit. Faktor rasio bahan:pelarut (1:5; 1:10; dan 1:15) dan lama waktu ekstraksi (5, 10, dan 15 menit) diamati agar diperoleh karakteristik ekstrak cabai rawit terbaik. Hasil penelitian menunjukkan bahwa rasio bahan:pelarut dan lama waktu ekstraksi memberikan pengaruh nyata terhadap kandungan capsaicinoid, total fenol dan flavonoid. Perlakuan terbaik metode ekstraksi UAE diperoleh dari perbandingan bahan:pelarut = 1:10 dan lama waktu ekstraksi 15 menit dengan kandungan capsaisin 102,89 mg/g bk, total fenol 56,75 mg GAE/g bk, total flavonoid 208,89 mg QE/g bk dan kandungan vitamin C 27,57 mg/g bk. ABSTRACT Chilli pepper (Capsicum frutenses L.) has high content of bioactive compounds such as capsaicinoid, phenol, flavonoids and vitamin C, so that potentially as a source of antioxidants. The aim of this study to determine the effect of materials:solvent and extraction time of ultrasonic assisted extraction (UAE) to phytochemical content and antioxidant activity of the chilli pepper extract. The ratio of material:solvent (1:5; 1:10; and 1:15) and time of extraction (5, 10, dan 15 min) were observed to obtain the best characteristics of chilli pepper extract. The results showed that the ratio of material:solvent and time of extraction gave a significant effect on capsaicinoid content, total phenol and flavonoids. The best treatment was obtained from the material:solvent ratio of 1:10 and extraction time of 15 minutes with capsaisin content of 102.89 mg/g dw, total phenol of 56.75 mg GAE/g dw, total flavonoid of 208.89 mg QE/g dw and vitamin C of 27.57 mg/g dw.

Genetic Diversity Study Among Six Genera of Amaranth Family Found in Malang Based on RAPD Marker Fatinah, Arik Arubil; Arumingtyas, Estri Laras; Mastuti, Retno
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Genera of amaranth family tend to have phenotypic variation partly caused by environmental factor. Phenotypic variation was the result of interaction between genetic and environmental factors. One of molecular markers that is widely used for detecting genetic variation is RAPD. RAPD is used for polymorphism detections and is now possible for identifiying a large number of loci and ascribes unambiguous taxonomic and genetic relationships among different taxa. Members of amaranth family found in Indonesia are Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, Gomphrena, Salsola, and Iresine. Six genera of which (Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, and Gomphrena) were observed in this study. DNA was extracted from fresh young leaves using Doyle and Doyles method with modification in the extraction buffer used. RAPD analyses were carried out with 20 decamer primers from Kit A of Operon Technology. DNA was amplified using master cycler gradient Eppendorf with 35 cycles. RAPD products were separated on 1,5 % agarose gels and detected by staining with ethidium bromide. There were 374 bands generated in 18 random primers. The number of monomorphic bands, polymorphic bands, and the percentage of polymorphism were 21 bands, 353 bands, and 94,38 % respectively. The high number and percentage of polymorphic bands revealed genomic DNA variation. This variation is in accordance with phenotypic variation detected in this experiment. Therefore, it can be concluded that, based on DNA polymorphism detected by RAPD, Amaranth family can be classified into two sub families namely Amaranthoideae and Gomphrenoideae.

Protein Expression of Soybean (Glycine Max L. Merr) Varieties In Drought Stress Savitri, Evika Sandi; Arumingtyas, Estri Laras
El-Hayah : Jurnal Biologi Vol 6, No 2 (2017): EL-HAYAH (VOL 6, NO 2,March 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i2.4822

Drought is one of the most severe limitations on the productivity of soybean. There are many genes and proteins involved in drought stress tolerance.Â Identification of proteins which could be used as the base for the development of molecular study is very important to understand drought tolerance thoroughly. The objective of the research was to investigate protein expression of soybean to drought stress. Changes in protein expression were analyzed using SDS PAGE and two dimensional gel electrophoresis. Image analysis of 2D protein was performed by using the PDQuest 8.0 software program (Bio-Rad). Tolerant variety, Dering-1, was subjected to drought stress using limitation of watering, while Detam-1, a sensitive variety, was used as comparator.Â The result showed that protein concentration have decreased in drought condition from 3,22 mg/ml to 0,77 mg/ml. The new protein band with the 24,95 kDa have been found in drought condition. This protein is osmotin like protein with the accession number NP915414 which may play a role in the mechanism of drought resistance. The identification of the protein based on sequence amino acid literature review

Conservation strategy of Anaphalis spp. in Bromo Tengger Semeru National Park, East Java Ade, Filza Yulina; Hakim, Luchman; Arumingtyas, Estri Laras; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 11, No 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/tls.11.01.10

The objective of the paper were to identify factors related to conservation strategy of Anaphalis in BTSNP. This study found internal factor related to strength includes the existence of species and value of Anaphalis spp., while the weaknesses include increase of habitat degradation and disturbance caused by tourism activities. The opportunities for conservation includes support of policy in biodiversity conservation and increase of ecotourism movement, while the threats aspect include less control of land uses changes and increase of mass tourism. The SWOT analyses recommends some significant strategy which are derived from combination of enhancing strength to optimizing opportunities, reducing weaknesses and optimizing opportunities, Enhancing strength to reduce threats and managing weaknesses to manage risk. Â

In Silico Analysis of rbcl Protein and D-Ribulose 1,5-bisphosphate Bond Ihwan, Ihwan; Sari, Dewi Ratih Tirto; Hakim, Luchman; Retnaningdyah, Catur; Arumingtyas, Estri Laras
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 2 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.02.65

The aim of this study was to determine the bond between the protein rbcL and D-Ribulose 1,5-bisphosphate by insilico. DNA sequences of the Mangrove Rhizophora mucronate rbcL gene DNA sequences from 7 different sources were obtained from NCBI for further alignment analysis using BioEdit software, phylogeny analysis using Mega6 software, molecular docking using PyRx software, preparation and visualization of docking results using Biovia Discovery Studio Visualizer software and analysis of the protein model quality based on the number of amino acid residues (Ramachandran plot analysis). The results of the docking molecular analysis showed interaction of 9 hydrogen bonds namely Asp203, Thr173, His294, Glu204, His327, Ser379, His298, Arg295, and Asn123 and 2 unfavorable bonds namely Lys177 and Lys175. This ligand and protein interaction complex was of good quality because the presence of amino acid residues in the most favored regions was greater than the amino acid residues in the disallowed regions outcomes

Constructing Phenetic and Phylogenetic Relationship Using Clad'97 Rahardi, Brian; Arumningtyas, Estri Laras; Mahmudi, Wayan Firdaus
Journal of Tropical Life Science Vol 2, No 1 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Relationship construction has a very important position in classification process for arranging taxonomy of organism. In the world of taxonomy, there are two the most familiar relationship diagram, cladogram and phenogram. In every construction activity, a researcher is always facing character state data from taxa that becomes components of the diagram. Calculation that is used for construction is often incorporate iterative or repetitive process that needs time and precision. The existence of calculating tools that produces both text and graphical output are hopefully decrease time and error during construction. Basic algorithm that is used in calculation is for phylogenetic construction by Kluge and Farris in 1969,for phenetic construction using cluster analysis with slight modification. Basic common algorithm used in the software is by calculating two dimensional arrays of taxa x characters matrix and creating distance or similarity matrix. In more detail the program creates one dimensional array of taxonomical object and each object has some other one dimensional array containing data commonly exist in a taxonomic unit. The relationship between one object and theother are regulated by an object that created by class representing taxonomic tree. Cladogram is constructed by calculating nearest distance between each taxon (OTU) and creating one HTU in every bifurcation. Phenogram is constructed agglomeratively by searching highest similarity between taxon then grouped into new taxon. Program calculates numerical data after we do character scoring. Final result for each user may be different; this may be due to decision by user during construction process. This paper hopefully attracts people from systematic computation to develop further into open source software and multi-platform feature.

Genetic variation of Dacrycarpus imbricatus in Bromo Tengger Semeru National Park, East Java Based on trnL (UAA) Intron Region Rahadiantoro, Apriyono; Hakim, Luchman; Arumingtyas, Estri Laras
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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The conservation of Jamuju Dacrycarpus imbricatus (Blume) de Laub. in Java Island has been considered important. One of the the limitation of such program is related to the viability data on the genetic diversity of species target. The aim of study was to determine genetic variation of D. imbricatus in Bromo Tengger Semeru Park, East Java based on trnL (UAA) intron region. DNA sample was collected from several D. imbricatus seedling population in Bromo Tengger Semeru National Park (BTSNP) in East Java. DNA was isolated and amplified using PCR. Genetic variation was estimated using trnL (UAA) intron sequences. This study confirm that D. imbricatus in Bromo Tengger Semeru has low genetic diversity. Based on the phylogenetic tree, D. imbricatus population from Bromo Tengger Semeru Park is closely related to D. imbricatus from Sabah-Malaysia and Hainan-China with 100 % similarity value. These data implies that population and habitat management of D. imbricatus in Bromo Tengger Semeru should be designed to enhance the population survival in the future.

Ethnobotanical Study of Postnatal Medicinal Plants in the Ethnic Madurese in Bangkalan Regency, East Java, Indonesia Siti Muslichah; Rodiyati Azrianingsih; Serafinah Indriyani; Estri Laras Arumingtyas
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15960

Postnatal period is a time for women to recover and take on new roles. Plants have an important role in the traditional posnatal care in many cultures around the world especially in rural areas. The use of medicinal plants is common among Madurese women especially to aid postpartum care. This research aimed to record the use of plants for postnatal care among the Madurese in Bangkalan Regency, East Java, Indonesia. Data were collected from local informants consisting of traditional birth attendants, herbal medicine makers, and herbal medicine sellers through open and semi-structured interviews. The data were analyzed quantitatively using the Use Value (UV), Family Use Value (FUV), and Index of Cultural Significance (ICS) parameters. There were 143 species of medicinal plants from 55 families identified as plants used for recovery during the postnatal period. Ginger is a plant that has the highest use value (1.84), followed by turmeric (1.81). There are five plant species which have very high ICS value. Ginger has the highest ICS value (168), followed by turmeric (132). The family with the highest FUV value was Zingiberaceae (0.79). Among the Madurese, medicinal plants are mostly used in multiherbal forms consisting of 2 to 30 plants. There are 13 kinds of concoctions used in postnatal care consisting of 8 multiherbal potions used orally and 5 herbs for external uses. Madurese women believe that the use of these herbs will speed up the postpartum recovery process and reduce postnatal diseases. This valuable knowledge should be continued to be applied by the young generation in order to maintain its existence and to conserve the plants used in this region.

Co-Authors A.A. Ketut Agung Cahyawan W Andayani, Dian Wuri Andy Soegianto Andy Soegianto Anggraeni In Oktavia Apriyono Rahadiantoro Apriyono Rahadiantoro, Apriyono Arik Arubil Fatinah Azrianingsih , Rodiyati Badiul Ulum Badiul Ulum Brian Rahardi Catur Retnaningdyah Dedi Dedi Dian Siswanto Dr.Ir. Yunianta, DEA Dwinianti, Edia Fitri Edi Priyo Utomo Elok Zubaidah Evika Sandi Savitri Fatinah, Arik Arubil Filza Yulina Ade Hakiki, Hilda Maghfirotu Hermalina Sinay Hernandi, Kevin Hohn Ihwan Ihwan Joni Kusnadi Juliandari, Ria Reinnata Khotibul Umam Al-Awwaly Luchman Hakim Mahmudi, Wayan Firdaus Mochammad Roviq Mochammad Roviq Monica, Rosina Nensy Marantika, Riza Nia Kurniawan Noor Hidayat, Noor Nunung Harijati Nur Basuki Nur Basuki Nur Istianah Retno Mastuti Ria Reinnata Juliandari Rifa'i, Muhaimin Rikza Hakin Rodiyati Azrianingsih Rodiyati Azrianingsih Rosyidah, A’liyatur Sari, Dewi Ratih Tirto Serafinah Indriyani Siti Muslichah Sri Widyarti Sundari Sundari Tatik Wardiyati Tatik Wardiyati Wahyu Widoretno Wayan Firdaus Mahmudi Zakiyah, 'Ainun Sayyidah

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