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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agroland : Jurnal Ilmu-Ilmu Pertanian Berkala Ilmiah Pertanian Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Tanaman Industri Jurnal Ilmu-Ilmu Pertanian Indonesia JURNAL AGROTEKNOLOGI Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian Indonesian Journal of Agricultural Research Jurnal Agro Wiralodra Jurnal Penelitian Tanaman Industri (Littri) Agroland: Jurnal Ilmu-ilmu Pertanian
Triwidodo Arwiyanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary

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Sebaran Penyakit Hawar Daun Bakteri di Beberapa Sentra Produksi Bawang Merah di Indonesia Asrul Asrul; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 18, No 1 (2013): February 2013
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v18i1.261

Abstract

Penelitian ini bertujuan mengetahui daerah sebaran penyakit hawar daun bakteri di beberapa sentra pertanaman bawang merah di Indonesia dan kultivar bawang merah yang dapat diinfeksi, serta mengidentifikasi patogen penyebabnya. Penentuan lokasi pengamatan dan pengambilan sampel dilakukan secara stratified purpossive random sampling. Survei dilakukan dengan cara wawancara dan pengamatan di lapangan (observasi) terhadap kultivar bawang dan gejala penyakit yang terinfeksi oleh bakteri patogen. Sampel diidentifikasi melalui pengamatan morfologi koloni, uji postulat Koch, uji reaksi hipersensitif dan pengujian sifat-sifat biokimia dan fisiologi. Hasil penelitian menunjukkan bahwa penyakit hawar daun bakteri telah tersebar secara merata di seluruh daerah pertanaman bawang merah di Indonesia, yang meliputi Kabupaten Cirebon, Tegal, Nganjuk, Bantul, dan Sigi, dengan tingkat serangan mencapai 62,5–100%. Penyakit ini menginfeksi bawang merah kultivar Bima curut, Bauji, Biru-sawah, dan Palasa. Gejala hawar daun bakteri yang dijumpai berupa water soaking, terjadi lekukan daun, pengerutan daun,  klorosis, nekrosis, mati pucuk, pertumbuhan kerdil, dan kematian. Isolat bakteri yang ditemukan mempunyai bentuk koloni bulat, cembung, berlendir, dan berwarna kuning. Ciri morfologi koloni, gejala dan karakteristik isolat bakteri mirip dengan sifat-sifat bakteri Xanthomonas axonopodis pv. allii penyebab penyakit hawar daun pada bawang bombay.Kata kunci: Sebaran, bawang merah hawar daun bakteri, Xanthomonas axonopodis pv. allii
Isolasi Bakteri Inti Es pada Kentang Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4663.558 KB) | DOI: 10.22146/jpti.9360

Abstract

Frost injury is one of the limiting factors in potato production in Dieng (2000 m above sea level), Central Java. The damage caused by frost has been recorded since long time ago, however, there is no studies about this matter. The objective of the present study is to isolate ice nucleation active bacteria which reside epiphytically at the surface of potato leaves. Potato and several crop leaves were collected and the ice catalyst was detected from leaf-washing. The bacteria were isolated on Nutrien Agar and Nutrien Agar Glycerol medium. The result indicated that ice catalyst was detected from the leaf washing of potato (Solanum tuberosum L.) and broad bean (Vicia faba L.) but not from others.  The ice catalyst was not detected from the extract of surface soil, neither. The ice nucleation active bacteria were successfully isolated from potato leaf and were identical to Erwinia ananas in their bacteriological properties. 
Pengendalian Hayati Penyakit Layu Bakteri Tembakau: 2. Percobaan di Rumah Kaca Triwidodo Arwiyanto; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 1 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9967

Abstract

Tobacco bacterial wilt caused by Ralstonia solanacearum has been controlled biologically in the greenhouse by the use of rhizosphere microorganisms, i.e. fluorescent pseudomonads and Bacillus spp. Root dipping for 30 minutes in the antagonist suspension (108 cfu/ml) suppressed bacterial wilt development. The best results were obtained with fluorescent pseudomonads Pf-20 and Bacillus sp. ba-118 isolates but the repression was annulled with the use of its combination. Disease severity were higher when the plants were treated with the combination between fluorescent pseudomonads. Treatment combinations between Pf-28, Pf-31, Pf-33, and Ba-118 could reduce the disease index.
Penapisan PGPF untuk Pengendalian Penyakit Busuk Lunak Lidah Buaya (Aloe vera) di Tanah Gambut Supriyanto Supriyanto; Achmadi Priyatmojo; Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11768

Abstract

Aloe (Aloe vera) planted in West Borneo peat soil is well known as having the best product quality in Indonesia. Soft rot disease is one of the constraints of Aloe cultivation on peat soil. Many methods have no significant result for controlling this disease. This research objectives are to obtain Plant Growth Promoting Fungi (PGPF) for controlling Aloe bacterial soft rot in peat soil. The research was conducted in Clinical Laboratory of Plant Pathology and glass house of Faculty of Agriculture, Gadjah Mada University, Yogyakarta from October 2008 to September 2009. The methods included fungal isolation from peat land, hypovirulent and PGPF ability test, and biological control test in the glass house. Among 42 peat soil fungi tested, 28 isolates were hypovirulent and only 2 isolates i.e. SNTH001 (Penicillium sp.) and SNTH003 (Aspergillus sp.) showed the PGPF ability. Glass house trial showed that single application of SNTH001 and SNTH003 isolates were able to increase the growth of Aloe. In the biological control of Aloe soft rot disease test showed that the lowest intensity (25%) might be obtained by using SNTH001 isolate.
Penekanan Penyakit Lincat Tembakau Temanggung dengan Streptomyces spp. Triwidodo Arwiyanto; Kharis Haryono; Achmadi Priyatmojo; Toekidjo Martoredjo; Gembong Dalmadiyo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11777

Abstract

Streptomyces is one of a soil microorganisms which is rarely used by Indonesian researcher for biological control of planth pathogent. Local isolates of Streptomyces spp. were used in this study to control "lincat disease" of Temanggung tobacco. Ninety four isolates of Streptomyces were tested directly in the field to suppress the development of lincat disease in the field. Six isolates, i.e. Stre-4, Stre-7,Stre-48, Stre-61, Stre-66 and Stre-67 suppressed the disease development with various degree. It was likely that decreasing the concentration of bacterial antagonist gave better protection against the disease. In vitro experiments indicated that there was no correlation between inhibition pathogen in vitro and ability to suppress disease in the field. The result showed that selection of antagonist in vitro based on antibiosis will eliminate the chance to get other potential antagonist.
Karakterisasi Parsial Streptomyces spp., Agens Pengendali Hayati Peyakit Lincat Tembakau Triwidodo Arwiyanto; Ari Astuti; Y. M. S. Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11854

Abstract

Local isolates of Streptomyces spp. were proven could suppress "lincat disease" of tobacco in the field. Six isolates were chosen for partial characterization of their bacteriological properties as based for the next experiments purposes. The results indicated that the isolates produce miselium with spore chains, gram positive, aerob, catalase and oxidase positive. The isolates also hydrolize starch, gelatine and esculine; produce lecithinase enzyme, reduce nitrate to nitrite, do not produce melanine pigment, did not produce hydrogen sulfide. The isolates were sensitive against streptomycine and rifampicin; able to use several carbon and nitrogen sources tested. Capable to grow on several medium pH, from 4,3 to 8,0. The isolates were able to grow from 5° C to 45° C; able to grow on medium containing 4% to 7% NaCl and ion the medium containing 0,1% of phenol. Plant pathogenicity test result showed negative responses which indicated that the used isolates were non plant pathogenic. The ability in suppressing lincat pathogen (Ralstonia solanacearum and Meloidogyne incognita) in vitro was vary between isolates.
Daya Tahan Hidup Pseudomonas putida Strain Pf-20 dalam Beberapa Macam Inokulum Yenny Wuryandari; Triwidodo Arwiyanto; Bambang Hadisutrisno; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12211

Abstract

For any crop-protection agent, an efficient formulation is a necessity to translate laboratory activity into adequate field performance. There are particular challenges to be faced in formulation of biological control agents, because the active ingredient is a living organism that must be kept relatively immobile and inactive while in storage, but quickly resume its  normal metabolic processes once applied to the target site. The objective of the research was  to study survival of Pseudomonas putida strain Pf-20 in various formulations at the storage  time and germination. The twelve formulations include carriers, additives and concentration  of Pf-20. The efficacy of various formulation in maintaining the population of Pf-20 in storage was assessed. The research result showed that population of Pf 20  in the formulation  number seven was the highest, with the combination peat+talc, CMC+arginin and  concentration of Pf[20 10^10 CFU/ml. In peat+talc, CMC+arginin, Pf-20 10^10  CFU/ml based formulation the bacteria survived even up to 84 days of storage although the population declined. In all formulations, population of Pf-20 increased at the time of seed germination. At the time of seed germination, formulation number seven was the highest too.
Deteksi Strain Pseudomonas solanacearum Penghasil Bakteriosin Triwidodo Arwiyanto; Sudarmadi Sudarmadi; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 2 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12890

Abstract

The capability of Pseudornonas solanacearum to produce bacteriocin was investigated with theuse oj Casamino-acid Peptone Glucose Agar. All strains tested produce bacteriocm withvarious spectra. Strain 6, 36 and ./9 inhibited almost all of the other strains. The avirulenttype of those three strains. i.e. 6-op. 36-op and -19-op, still produced bacteriocln on the samemedium. The.concentratlon of carbon source and acidity of the medium did not have any effecton the bacteriocin production. The bacterioctn produced by strains 6-op, 36-op and 49-opwere inactivated at 70°C for 30 minutes.
Pengendalian Hayati Penyakit Layu Bakteri Tembakau: 1. Isolasi Bakteri Antagonis Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 1 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12910

Abstract

Three hundred strains of fluorescent pseudomonad and 120 strains of Bacillus spp. were isolated from the rhizosphere of Mimosa invisa L. Fluorescent pseudomonad was isolated on King s B while Bacillus spp. were isolated on Tryptic Soy Agar medium. Each media were supplemented with 100 ppm cycloheximide to suppress fungal growth. All isolated strains were tested for their capability to suppress the growth of Pseudomonas solanacearum on appropiate media. Most of fluorescent pseudomonad inhibited the growth of the pathogen with an inhibition zone from 1 mm to 16 mm. The mechanism of growth-inhibition was bacteriostatic and some of them were bactericidal. Sixty six out of 120 strains of Bacillus spp. produced defined inhibition zones on the media. The zone of growth-inhibition varied from 2 to 14 mm and the mechanism of inhibition was bactericidal. Several strains of those two bacterial marga exhibited non-antagonistic activity toward each other.
Dinamika Populasi Pseudomonas solanacearum pada Rizosfer Tanaman Bukan Inang Triwidodo Arwiyanto; Elizabeth Handini; Toekidjo Martoredjo
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 2 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12962

Abstract

Bacterial wilt caused by Pseudomonas solanacearum is a main limiting factor in the production of tobacco. The objective of this study is to investigate the survival of P. solanacearum in the rhizosphere of presumed nonhost plants. The results indicated that in the sugarcane rhizosphere, the pathogen population decreased along with the time course. The bacteria could not infect the root of sugarcane. On the other hand, the pathogen could infect the root of Mimosa invisa, although the population also decreased along with the time course.
Co-Authors . Supriyanto Achmadi Priyatmojo Alifia Nur Ayusma Anggita Rahmawati Ani Widiastuti, Ani Ari Astuti Arif Wibowo Asrul Asrul Asrul Asrul Asrul Azizah Ridha Ulilalbab Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hendro Sunarminto Bambang Trianom CHRISNAWATI CHRISNAWATI CHRISTANTI CHRISTANTI Christanti Sumardiyono Didik Indradewa Didik Indradewa Djati Widhityarini Dwimartina, Fina Elizabeth Handini Gembong Dalmadiyo Heru Adi Djatmiko I. Hartana I. Hartana IKA MARISKA Irpawa, Dea Musytari Intan Isna Maulida JAKA WIDADA Jaka Widada Jaka Widada Kharis Haryono Lisa Navitasari Murti, Rudi Hari NASRUN NASRUN NASRUN NASRUN Nur Prihatiningsih Putri Laeshita Riska Awalia Putri Rudi Hari Murti Saputra, Rachmad SEDYO HARTONO Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sudarmadi Sudarmadi Suhartiningsih Dwi Nurcahyanti Tati Suharti Tati Suharti Toekidjo Martoredjo Toekidjo Martoredjo Tri Joko Tri Joko Tri Joko Tri Joko Tri Retno Widyastuti Wulandari, Rani Agustina Y. M. S. Maryudani Yenny WURYANDARI